Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco
Tuberculosis is a major public health threat, annually affecting new individuals worldwide, especially those in developing countries. Rapid detection of the agent and effective treatment are two important factors in controlling this disease. The present study aimed to evaluate polymerase chain react...
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| Veröffentlicht in: | Journal of infection in developing countries 2012-01, Vol.6 (1), p.40-45 |
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| creator | Zakham, Fathiah Bazoui, Halima Akrim, Mohammed Lemrabet, Sanae Lahlou, Ouafae Elmzibri, Mohamed Benjouad, Abdelaziz Ennaji, My Mustapha Elaouad, Rajae |
| description | Tuberculosis is a major public health threat, annually affecting new individuals worldwide, especially those in developing countries. Rapid detection of the agent and effective treatment are two important factors in controlling this disease.
The present study aimed to evaluate polymerase chain reaction (PCR) as a rapid and direct molecular method for the diagnosis of Mycobacterium tuberculosis (MTB) in 70 clinical specimens (62 sputum samples, six cerebrospinal fluids, and two biopsies) using heat shock protein (hsp65) as the gene target. Automated sequencing of the same gene was used for the identification of MTB to the species level.
The sensitivity of PCR was 81.13%, with specificity of 88.24%; the positive and negative predictive values were 95.56% and 60%, respectively.
Based on these results, the hsp65 gene sequence can be used to differentiate the members of MTB complex from non-tuberculosis mycobacteria (NTM). |
| doi_str_mv | 10.3855/jidc.1857 |
| format | Article |
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The present study aimed to evaluate polymerase chain reaction (PCR) as a rapid and direct molecular method for the diagnosis of Mycobacterium tuberculosis (MTB) in 70 clinical specimens (62 sputum samples, six cerebrospinal fluids, and two biopsies) using heat shock protein (hsp65) as the gene target. Automated sequencing of the same gene was used for the identification of MTB to the species level.
The sensitivity of PCR was 81.13%, with specificity of 88.24%; the positive and negative predictive values were 95.56% and 60%, respectively.
Based on these results, the hsp65 gene sequence can be used to differentiate the members of MTB complex from non-tuberculosis mycobacteria (NTM).</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.1857</identifier><identifier>PMID: 22240427</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Base Sequence ; Cerebrospinal Fluid - microbiology ; Chaperonin 60 - chemistry ; Chaperonin 60 - genetics ; Humans ; Molecular Sequence Data ; Morocco ; Mycobacterium tuberculosis - classification ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Polymerase Chain Reaction - methods ; Predictive Value of Tests ; Sensitivity and Specificity ; Sequence Analysis, DNA ; Sputum - microbiology ; Tuberculosis ; Tuberculosis, Pulmonary - diagnosis ; Tuberculosis, Pulmonary - microbiology ; Tuberculosis, Spinal - diagnosis ; Tuberculosis, Spinal - microbiology</subject><ispartof>Journal of infection in developing countries, 2012-01, Vol.6 (1), p.40-45</ispartof><rights>2012. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c312t-7520ee2d86ac9c185bb6c1e81e318329b4d278341d89aa1f323745aeac9aaae3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22240427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zakham, Fathiah</creatorcontrib><creatorcontrib>Bazoui, Halima</creatorcontrib><creatorcontrib>Akrim, Mohammed</creatorcontrib><creatorcontrib>Lemrabet, Sanae</creatorcontrib><creatorcontrib>Lahlou, Ouafae</creatorcontrib><creatorcontrib>Elmzibri, Mohamed</creatorcontrib><creatorcontrib>Benjouad, Abdelaziz</creatorcontrib><creatorcontrib>Ennaji, My Mustapha</creatorcontrib><creatorcontrib>Elaouad, Rajae</creatorcontrib><title>Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco</title><title>Journal of infection in developing countries</title><addtitle>J Infect Dev Ctries</addtitle><description>Tuberculosis is a major public health threat, annually affecting new individuals worldwide, especially those in developing countries. Rapid detection of the agent and effective treatment are two important factors in controlling this disease.
The present study aimed to evaluate polymerase chain reaction (PCR) as a rapid and direct molecular method for the diagnosis of Mycobacterium tuberculosis (MTB) in 70 clinical specimens (62 sputum samples, six cerebrospinal fluids, and two biopsies) using heat shock protein (hsp65) as the gene target. Automated sequencing of the same gene was used for the identification of MTB to the species level.
The sensitivity of PCR was 81.13%, with specificity of 88.24%; the positive and negative predictive values were 95.56% and 60%, respectively.
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Rapid detection of the agent and effective treatment are two important factors in controlling this disease.
The present study aimed to evaluate polymerase chain reaction (PCR) as a rapid and direct molecular method for the diagnosis of Mycobacterium tuberculosis (MTB) in 70 clinical specimens (62 sputum samples, six cerebrospinal fluids, and two biopsies) using heat shock protein (hsp65) as the gene target. Automated sequencing of the same gene was used for the identification of MTB to the species level.
The sensitivity of PCR was 81.13%, with specificity of 88.24%; the positive and negative predictive values were 95.56% and 60%, respectively.
Based on these results, the hsp65 gene sequence can be used to differentiate the members of MTB complex from non-tuberculosis mycobacteria (NTM).</abstract><cop>Italy</cop><pub>Journal of Infection in Developing Countries</pub><pmid>22240427</pmid><doi>10.3855/jidc.1857</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of infection in developing countries, 2012-01, Vol.6 (1), p.40-45 |
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| subjects | Bacterial Proteins - chemistry Bacterial Proteins - genetics Base Sequence Cerebrospinal Fluid - microbiology Chaperonin 60 - chemistry Chaperonin 60 - genetics Humans Molecular Sequence Data Morocco Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Polymerase Chain Reaction - methods Predictive Value of Tests Sensitivity and Specificity Sequence Analysis, DNA Sputum - microbiology Tuberculosis Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - microbiology Tuberculosis, Spinal - diagnosis Tuberculosis, Spinal - microbiology |
| title | Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco |
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