Immobilization of fluorous oligosaccharide recognized by influenza virus on polytetrafluoroethylene filter

The lactoside with PEG-fluorous tag was introduced to BHK-21(C-13) cells to generate a GM3-type oligosaccharide (Siaα2-3Galβ1-4Glc). The GM3-type oligosaccharide obtained was easily immobilized by spotting onto commercially available polytetrafluoroethylene (PTFE) filter through non-covalent fluorou...

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Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2012-01, Vol.22 (2), p.1251-1254
Hauptverfasser:	Tojino, Mami, Mori, Masako, Kasuya, Maria Carmelita Z., Hatanaka, Kenichi, Kawaguchi, Atsushi, Nagata, Kyosuke, Shirai, Takashi, Mizuno, Mamoru
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Line Cricetinae Fluorous Influenza A virus - chemistry Influenza virus Medical sciences membrane proteins Membranes, Artificial Oligosaccharide oligosaccharides Oligosaccharides - chemistry Orthomyxoviridae Pharmacology. Drug treatments Polyethylene Glycols - chemistry polytetrafluoroethylene Polytetrafluoroethylene - chemistry Polytetrafluoroethylene filter Saccharide primer sialic acids viruses
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Beschreibung
Zusammenfassung:	The lactoside with PEG-fluorous tag was introduced to BHK-21(C-13) cells to generate a GM3-type oligosaccharide (Siaα2-3Galβ1-4Glc). The GM3-type oligosaccharide obtained was easily immobilized by spotting onto commercially available polytetrafluoroethylene (PTFE) filter through non-covalent fluorous affinity and simply assessed by dot blot method using the interaction of carbohydrate- with proteins which recognize sialic acid such as virus membrane proteins.
ISSN:	0960-894X 1464-3405
DOI:	10.1016/j.bmcl.2011.11.057