LC‐MS/MS quantification of short‐chain acyl‐CoA’s in Escherichia coli demonstrates versatile propionyl‐CoA synthetase substrate specificity

Aims: This paper utilized quantitative LC‐MS/MS to profile the short‐chain acyl‐CoA levels of several strains of Escherichia coli engineered for heterologous polyketide production. To further compare and potentially expand the levels of available acyl‐CoA molecules, a propionyl‐CoA synthetase gene f...

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