Molecular cloning and characterization of the STAT gene in Hyphantria cunea haemocytes
A new insect member of the signal transducer and activator of transcription (STAT) family of transcription factors, Hyphantria cunea STAT (HcSTAT), was cloned from the lepidopteran H. cunea. The domain involved in DNA interaction and the Src homology 2 (SH2) domain were well conserved. During all de...
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| Veröffentlicht in: | Insect molecular biology 2011-12, Vol.20 (6), p.723-732 |
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| creator | Kim, H. J Kwon, Y. M Kim, Y. I Lee, I. H Jin, B. R Han, Y. S Cheon, H. M Kang, Y. J Seo, S. J |
| description | A new insect member of the signal transducer and activator of transcription (STAT) family of transcription factors, Hyphantria cunea STAT (HcSTAT), was cloned from the lepidopteran H. cunea. The domain involved in DNA interaction and the Src homology 2 (SH2) domain were well conserved. During all developmental stages, the gene was expressed at a low level in the haemocytes, fat body cells, midgut, epidermis and Malpighian tubules. The haemocytes and Malpighian tubules showed transcriptional activation of HcSTAT upon Gram‐negative and Gram‐positive bacterial challenges. These challenges increased the induction and nuclear translocation of the HcSTAT protein that recognizes a STAT target site in H. cunea haemocytes. In vivo treatment with sodium orthovanadate translocated HcSTAT to the haemocyte nucleus. This study shows the involvement of the haemocyte Janus kinase/STAT pathway after microbial infection in lepidopteran insects. |
| doi_str_mv | 10.1111/j.1365-2583.2011.01102.x |
| format | Article |
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J ; Kwon, Y. M ; Kim, Y. I ; Lee, I. H ; Jin, B. R ; Han, Y. S ; Cheon, H. M ; Kang, Y. J ; Seo, S. J</creator><creatorcontrib>Kim, H. J ; Kwon, Y. M ; Kim, Y. I ; Lee, I. H ; Jin, B. R ; Han, Y. S ; Cheon, H. M ; Kang, Y. J ; Seo, S. J</creatorcontrib><description>A new insect member of the signal transducer and activator of transcription (STAT) family of transcription factors, Hyphantria cunea STAT (HcSTAT), was cloned from the lepidopteran H. cunea. The domain involved in DNA interaction and the Src homology 2 (SH2) domain were well conserved. During all developmental stages, the gene was expressed at a low level in the haemocytes, fat body cells, midgut, epidermis and Malpighian tubules. The haemocytes and Malpighian tubules showed transcriptional activation of HcSTAT upon Gram‐negative and Gram‐positive bacterial challenges. These challenges increased the induction and nuclear translocation of the HcSTAT protein that recognizes a STAT target site in H. cunea haemocytes. In vivo treatment with sodium orthovanadate translocated HcSTAT to the haemocyte nucleus. This study shows the involvement of the haemocyte Janus kinase/STAT pathway after microbial infection in lepidopteran insects.</description><identifier>ISSN: 0962-1075</identifier><identifier>EISSN: 1365-2583</identifier><identifier>DOI: 10.1111/j.1365-2583.2011.01102.x</identifier><identifier>PMID: 21958414</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Candida albicans - immunology ; Cloning, Molecular ; Developmental stages ; DNA ; Epidermis ; Escherichia coli - immunology ; Fat body ; genes ; Genes, Insect ; haemocyte ; Hemocytes ; Hemocytes - metabolism ; Hemocytes - microbiology ; Homology ; Hyphantria cunea ; Infection ; Janus kinase ; Larva - genetics ; Larva - immunology ; Larva - microbiology ; Lepidoptera ; Malpighian tubules ; Micrococcus luteus - immunology ; Midgut ; molecular cloning ; Moths - genetics ; Moths - immunology ; Moths - microbiology ; non-specific protein-tyrosine kinase ; Nuclear transport ; Pupa - genetics ; Pupa - immunology ; Pupa - microbiology ; RNA, Messenger - metabolism ; Sequence Analysis, DNA ; SH2 domain ; signal transducers and activators of transcription (STATs) ; sodium ; sodium orthovanadate ; Src protein ; STAT Transcription Factors - genetics ; STAT Transcription Factors - isolation & purification ; STAT Transcription Factors - metabolism ; Transcription activation ; Transcription factors ; transcriptional activation</subject><ispartof>Insect molecular biology, 2011-12, Vol.20 (6), p.723-732</ispartof><rights>2011 The Authors. Insect Molecular Biology © 2011 The Royal Entomological Society</rights><rights>2011 The Authors. Insect Molecular Biology © 2011 The Royal Entomological Society.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5132-558c13362122022dab024408c9fdbe0270fd2fac5d11620b31f87be790ebea663</citedby><cites>FETCH-LOGICAL-c5132-558c13362122022dab024408c9fdbe0270fd2fac5d11620b31f87be790ebea663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2583.2011.01102.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2583.2011.01102.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21958414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, H. J</creatorcontrib><creatorcontrib>Kwon, Y. M</creatorcontrib><creatorcontrib>Kim, Y. I</creatorcontrib><creatorcontrib>Lee, I. H</creatorcontrib><creatorcontrib>Jin, B. R</creatorcontrib><creatorcontrib>Han, Y. S</creatorcontrib><creatorcontrib>Cheon, H. M</creatorcontrib><creatorcontrib>Kang, Y. J</creatorcontrib><creatorcontrib>Seo, S. J</creatorcontrib><title>Molecular cloning and characterization of the STAT gene in Hyphantria cunea haemocytes</title><title>Insect molecular biology</title><addtitle>Insect Mol Biol</addtitle><description>A new insect member of the signal transducer and activator of transcription (STAT) family of transcription factors, Hyphantria cunea STAT (HcSTAT), was cloned from the lepidopteran H. cunea. The domain involved in DNA interaction and the Src homology 2 (SH2) domain were well conserved. During all developmental stages, the gene was expressed at a low level in the haemocytes, fat body cells, midgut, epidermis and Malpighian tubules. The haemocytes and Malpighian tubules showed transcriptional activation of HcSTAT upon Gram‐negative and Gram‐positive bacterial challenges. These challenges increased the induction and nuclear translocation of the HcSTAT protein that recognizes a STAT target site in H. cunea haemocytes. In vivo treatment with sodium orthovanadate translocated HcSTAT to the haemocyte nucleus. This study shows the involvement of the haemocyte Janus kinase/STAT pathway after microbial infection in lepidopteran insects.</description><subject>Animals</subject><subject>Candida albicans - immunology</subject><subject>Cloning, Molecular</subject><subject>Developmental stages</subject><subject>DNA</subject><subject>Epidermis</subject><subject>Escherichia coli - immunology</subject><subject>Fat body</subject><subject>genes</subject><subject>Genes, Insect</subject><subject>haemocyte</subject><subject>Hemocytes</subject><subject>Hemocytes - metabolism</subject><subject>Hemocytes - microbiology</subject><subject>Homology</subject><subject>Hyphantria cunea</subject><subject>Infection</subject><subject>Janus kinase</subject><subject>Larva - genetics</subject><subject>Larva - immunology</subject><subject>Larva - microbiology</subject><subject>Lepidoptera</subject><subject>Malpighian tubules</subject><subject>Micrococcus luteus - immunology</subject><subject>Midgut</subject><subject>molecular cloning</subject><subject>Moths - genetics</subject><subject>Moths - immunology</subject><subject>Moths - microbiology</subject><subject>non-specific protein-tyrosine kinase</subject><subject>Nuclear transport</subject><subject>Pupa - genetics</subject><subject>Pupa - immunology</subject><subject>Pupa - microbiology</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>SH2 domain</subject><subject>signal transducers and activators of transcription (STATs)</subject><subject>sodium</subject><subject>sodium orthovanadate</subject><subject>Src protein</subject><subject>STAT Transcription Factors - genetics</subject><subject>STAT Transcription Factors - isolation & purification</subject><subject>STAT Transcription Factors - metabolism</subject><subject>Transcription activation</subject><subject>Transcription factors</subject><subject>transcriptional activation</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkkFv0zAYhi0EYmXwF8DiwinB_uI48YHDNtFtYgPBOjhajuO0LqlT7ERr9-txyOiBA5oly5b8vK_06TFCmJKUxvV-ndKM5wnkZZYCoTSNm0C6e4Jmh4enaEYEh4SSIj9CL0JYE0JKwcVzdARU5CWjbIa-X3et0UOrPNZt56xbYuVqrFfKK90bb-9VbzuHuwb3K4NvFicLvDTOYOvwxX67Uq73VmE9OKPwSplNp_e9CS_Rs0a1wbx6OI_R7fzj4uwiufpyfnl2cpXonGaQ5HmpaZZxoAAEoFYVAcZIqUVTV4ZAQZoaGqXzmlIOpMpoUxaVKQQxlVGcZ8fo3dS79d2vwYRebmzQpm2VM90QpKA5E8A4fQRJBRScQSTf_kOuu8G7OIYUBDhwkZEIlROkfReCN43certRfi8pkaMjuZajCjmqkKMj-ceR3MXo64f-odqY-hD8KyUCHybgzrZm_-hieXl9Ot5iPpnyNvRmd8gr_1PyIity-ePzufz6qSBszr7JeeTfTHyjOqmW3gZ5exObWfwwjHPG_ktAyQjLfgN9Xr7K</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Kim, H. J</creator><creator>Kwon, Y. M</creator><creator>Kim, Y. I</creator><creator>Lee, I. H</creator><creator>Jin, B. R</creator><creator>Han, Y. S</creator><creator>Cheon, H. M</creator><creator>Kang, Y. J</creator><creator>Seo, S. J</creator><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201112</creationdate><title>Molecular cloning and characterization of the STAT gene in Hyphantria cunea haemocytes</title><author>Kim, H. J ; Kwon, Y. M ; Kim, Y. I ; Lee, I. H ; Jin, B. R ; Han, Y. S ; Cheon, H. M ; Kang, Y. J ; Seo, S. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5132-558c13362122022dab024408c9fdbe0270fd2fac5d11620b31f87be790ebea663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Candida albicans - immunology</topic><topic>Cloning, Molecular</topic><topic>Developmental stages</topic><topic>DNA</topic><topic>Epidermis</topic><topic>Escherichia coli - immunology</topic><topic>Fat body</topic><topic>genes</topic><topic>Genes, Insect</topic><topic>haemocyte</topic><topic>Hemocytes</topic><topic>Hemocytes - metabolism</topic><topic>Hemocytes - microbiology</topic><topic>Homology</topic><topic>Hyphantria cunea</topic><topic>Infection</topic><topic>Janus kinase</topic><topic>Larva - genetics</topic><topic>Larva - immunology</topic><topic>Larva - microbiology</topic><topic>Lepidoptera</topic><topic>Malpighian tubules</topic><topic>Micrococcus luteus - immunology</topic><topic>Midgut</topic><topic>molecular cloning</topic><topic>Moths - genetics</topic><topic>Moths - immunology</topic><topic>Moths - microbiology</topic><topic>non-specific protein-tyrosine kinase</topic><topic>Nuclear transport</topic><topic>Pupa - genetics</topic><topic>Pupa - immunology</topic><topic>Pupa - microbiology</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>SH2 domain</topic><topic>signal transducers and activators of transcription (STATs)</topic><topic>sodium</topic><topic>sodium orthovanadate</topic><topic>Src protein</topic><topic>STAT Transcription Factors - genetics</topic><topic>STAT Transcription Factors - isolation & purification</topic><topic>STAT Transcription Factors - metabolism</topic><topic>Transcription activation</topic><topic>Transcription factors</topic><topic>transcriptional activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, H. J</creatorcontrib><creatorcontrib>Kwon, Y. M</creatorcontrib><creatorcontrib>Kim, Y. I</creatorcontrib><creatorcontrib>Lee, I. H</creatorcontrib><creatorcontrib>Jin, B. R</creatorcontrib><creatorcontrib>Han, Y. S</creatorcontrib><creatorcontrib>Cheon, H. M</creatorcontrib><creatorcontrib>Kang, Y. J</creatorcontrib><creatorcontrib>Seo, S. J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, H. J</au><au>Kwon, Y. M</au><au>Kim, Y. I</au><au>Lee, I. H</au><au>Jin, B. R</au><au>Han, Y. S</au><au>Cheon, H. M</au><au>Kang, Y. J</au><au>Seo, S. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and characterization of the STAT gene in Hyphantria cunea haemocytes</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2011-12</date><risdate>2011</risdate><volume>20</volume><issue>6</issue><spage>723</spage><epage>732</epage><pages>723-732</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>A new insect member of the signal transducer and activator of transcription (STAT) family of transcription factors, Hyphantria cunea STAT (HcSTAT), was cloned from the lepidopteran H. cunea. The domain involved in DNA interaction and the Src homology 2 (SH2) domain were well conserved. During all developmental stages, the gene was expressed at a low level in the haemocytes, fat body cells, midgut, epidermis and Malpighian tubules. The haemocytes and Malpighian tubules showed transcriptional activation of HcSTAT upon Gram‐negative and Gram‐positive bacterial challenges. These challenges increased the induction and nuclear translocation of the HcSTAT protein that recognizes a STAT target site in H. cunea haemocytes. In vivo treatment with sodium orthovanadate translocated HcSTAT to the haemocyte nucleus. This study shows the involvement of the haemocyte Janus kinase/STAT pathway after microbial infection in lepidopteran insects.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21958414</pmid><doi>10.1111/j.1365-2583.2011.01102.x</doi><tpages>10</tpages></addata></record> |
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| ispartof | Insect molecular biology, 2011-12, Vol.20 (6), p.723-732 |
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| subjects | Animals Candida albicans - immunology Cloning, Molecular Developmental stages DNA Epidermis Escherichia coli - immunology Fat body genes Genes, Insect haemocyte Hemocytes Hemocytes - metabolism Hemocytes - microbiology Homology Hyphantria cunea Infection Janus kinase Larva - genetics Larva - immunology Larva - microbiology Lepidoptera Malpighian tubules Micrococcus luteus - immunology Midgut molecular cloning Moths - genetics Moths - immunology Moths - microbiology non-specific protein-tyrosine kinase Nuclear transport Pupa - genetics Pupa - immunology Pupa - microbiology RNA, Messenger - metabolism Sequence Analysis, DNA SH2 domain signal transducers and activators of transcription (STATs) sodium sodium orthovanadate Src protein STAT Transcription Factors - genetics STAT Transcription Factors - isolation & purification STAT Transcription Factors - metabolism Transcription activation Transcription factors transcriptional activation |
| title | Molecular cloning and characterization of the STAT gene in Hyphantria cunea haemocytes |
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