AFLP Marking and Polymorphism among Progenies of Gymnema sylvestre: an Important Medicinal Plant of India
The level of polymorphism among twelve selected progenies of Gymnema sylvestre was investigated through AFLP markers by multiplexing PCR reactions using 64 (8×8) primer combinations. Fourteen primer combinations were selected as the most suitable combination for G. sylvestre. Analysis of the 12 prog...
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| Veröffentlicht in: | Natural product communications 2011-11, Vol.6 (11), p.1679-1682 |
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| creator | Osman, Magda Abbaker Dhawan, Sunita Singh Bahl, Janak Raj Darokar, Mahendra P Khanuja, Suman P S |
| description | The level of polymorphism among twelve selected progenies of Gymnema sylvestre was investigated through AFLP markers by multiplexing PCR reactions using 64 (8×8) primer combinations. Fourteen primer combinations were selected as the most suitable combination for G. sylvestre. Analysis of the 12 progenies with these 14 primer pairs produced 1689 fragments of which 972 (57.5%) were polymorphic and 485 (28.7%) were unique to a particular genotype. The number of fragments produced by individual primer pairs was in the range of 55 to 225. Out of these, polymorphic fragments were in the range of 34 (E-ACC/M-CAC) to 157 (E-AGG/M-CAG) and unique bands observed were 8 (E-ACC / M-CAC) to 69 (E-AGG/M-CAC). Different primer combinations detected different levels of polymorphism, ranging from 33% (E-AGG/ M-CAC) to 69.8% (E-AGG/ M-CAC). From the observations, it appears that the primer combinations E-AGG/M-CAC, E-AGG/CTG, E-AGG/CAG and E-ACA / CAT were the most informative for the detection of polymorphism among the progenies compared with others, since they produced a high number of unique fragments. The similarity coefficient ranged from 0.212 to 0.731. High similarity was observed between progeny S8 and S9 (73%) and high divergence between progenies S3 and S11. Among the selected progeny, S9 was found to be the most similar to the parent (63%), while genotype S11 was the most distant (36.9%). |
| doi_str_mv | 10.1177/1934578X1100601129 |
| format | Article |
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Fourteen primer combinations were selected as the most suitable combination for G. sylvestre. Analysis of the 12 progenies with these 14 primer pairs produced 1689 fragments of which 972 (57.5%) were polymorphic and 485 (28.7%) were unique to a particular genotype. The number of fragments produced by individual primer pairs was in the range of 55 to 225. Out of these, polymorphic fragments were in the range of 34 (E-ACC/M-CAC) to 157 (E-AGG/M-CAG) and unique bands observed were 8 (E-ACC / M-CAC) to 69 (E-AGG/M-CAC). Different primer combinations detected different levels of polymorphism, ranging from 33% (E-AGG/ M-CAC) to 69.8% (E-AGG/ M-CAC). From the observations, it appears that the primer combinations E-AGG/M-CAC, E-AGG/CTG, E-AGG/CAG and E-ACA / CAT were the most informative for the detection of polymorphism among the progenies compared with others, since they produced a high number of unique fragments. The similarity coefficient ranged from 0.212 to 0.731. High similarity was observed between progeny S8 and S9 (73%) and high divergence between progenies S3 and S11. Among the selected progeny, S9 was found to be the most similar to the parent (63%), while genotype S11 was the most distant (36.9%).</description><identifier>ISSN: 1934-578X</identifier><identifier>EISSN: 1555-9475</identifier><identifier>DOI: 10.1177/1934578X1100601129</identifier><identifier>PMID: 22224288</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Amplified Fragment Length Polymorphism Analysis ; Cluster Analysis ; DNA Primers ; Gymnema sylvestre - genetics ; India ; Plants, Medicinal - genetics ; Polymorphism, Genetic</subject><ispartof>Natural product communications, 2011-11, Vol.6 (11), p.1679-1682</ispartof><rights>2011 SAGE Publications Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-ad72d26ef54077e0815f6704c7c01d6978a3243e2295f984983f962ce8079d213</citedby><cites>FETCH-LOGICAL-c386t-ad72d26ef54077e0815f6704c7c01d6978a3243e2295f984983f962ce8079d213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1934578X1100601129$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1934578X1100601129$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21966,27853,27924,27925,44945,45333</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/1934578X1100601129?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22224288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Osman, Magda Abbaker</creatorcontrib><creatorcontrib>Dhawan, Sunita Singh</creatorcontrib><creatorcontrib>Bahl, Janak Raj</creatorcontrib><creatorcontrib>Darokar, Mahendra P</creatorcontrib><creatorcontrib>Khanuja, Suman P S</creatorcontrib><title>AFLP Marking and Polymorphism among Progenies of Gymnema sylvestre: an Important Medicinal Plant of India</title><title>Natural product communications</title><addtitle>Nat Prod Commun</addtitle><description>The level of polymorphism among twelve selected progenies of Gymnema sylvestre was investigated through AFLP markers by multiplexing PCR reactions using 64 (8×8) primer combinations. Fourteen primer combinations were selected as the most suitable combination for G. sylvestre. Analysis of the 12 progenies with these 14 primer pairs produced 1689 fragments of which 972 (57.5%) were polymorphic and 485 (28.7%) were unique to a particular genotype. The number of fragments produced by individual primer pairs was in the range of 55 to 225. Out of these, polymorphic fragments were in the range of 34 (E-ACC/M-CAC) to 157 (E-AGG/M-CAG) and unique bands observed were 8 (E-ACC / M-CAC) to 69 (E-AGG/M-CAC). Different primer combinations detected different levels of polymorphism, ranging from 33% (E-AGG/ M-CAC) to 69.8% (E-AGG/ M-CAC). From the observations, it appears that the primer combinations E-AGG/M-CAC, E-AGG/CTG, E-AGG/CAG and E-ACA / CAT were the most informative for the detection of polymorphism among the progenies compared with others, since they produced a high number of unique fragments. The similarity coefficient ranged from 0.212 to 0.731. High similarity was observed between progeny S8 and S9 (73%) and high divergence between progenies S3 and S11. Among the selected progeny, S9 was found to be the most similar to the parent (63%), while genotype S11 was the most distant (36.9%).</description><subject>Amplified Fragment Length Polymorphism Analysis</subject><subject>Cluster Analysis</subject><subject>DNA Primers</subject><subject>Gymnema sylvestre - genetics</subject><subject>India</subject><subject>Plants, Medicinal - genetics</subject><subject>Polymorphism, Genetic</subject><issn>1934-578X</issn><issn>1555-9475</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LwzAYxoMobsx9AQ-Sm6e6JE2axNsYbg427EHBW4ltOjObZiar0G9vxqYXwffy_uH3PLw8AFxjdIcx5xMsU8q4eMUYoQxhTOQZGGLGWCIpZ-dxjkByIAZgHMIWxRKCIiovwYDEokSIITDT-SqHa-U_TLuBqq1g7preOr97N8FCZV08595tdGt0gK6Gi9622ioY-uZLh73X91EGl3bn_F61e7jWlSlNqxqYN4c9SpZtZdQVuKhVE_T41EfgZf7wPHtMVk-L5Wy6SspUZPtEVZxUJNM1o4hzjQRmdcYRLXmJcJVJLlRKaKoJkayWgkqR1jIjpRaIy4rgdARuj7477z67-GFhTSh1E5_RrguFxAxRhLMDSY5k6V0IXtfFzhurfF9gVBxCLv6GHEU3J_vuzerqV_ITaQQmRyCojS62rvMxi_Cf5TemdYNX</recordid><startdate>201111</startdate><enddate>201111</enddate><creator>Osman, Magda Abbaker</creator><creator>Dhawan, Sunita Singh</creator><creator>Bahl, Janak Raj</creator><creator>Darokar, Mahendra P</creator><creator>Khanuja, Suman P S</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201111</creationdate><title>AFLP Marking and Polymorphism among Progenies of Gymnema sylvestre: an Important Medicinal Plant of India</title><author>Osman, Magda Abbaker ; Dhawan, Sunita Singh ; Bahl, Janak Raj ; Darokar, Mahendra P ; Khanuja, Suman P S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-ad72d26ef54077e0815f6704c7c01d6978a3243e2295f984983f962ce8079d213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amplified Fragment Length Polymorphism Analysis</topic><topic>Cluster Analysis</topic><topic>DNA Primers</topic><topic>Gymnema sylvestre - genetics</topic><topic>India</topic><topic>Plants, Medicinal - genetics</topic><topic>Polymorphism, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osman, Magda Abbaker</creatorcontrib><creatorcontrib>Dhawan, Sunita Singh</creatorcontrib><creatorcontrib>Bahl, Janak Raj</creatorcontrib><creatorcontrib>Darokar, Mahendra P</creatorcontrib><creatorcontrib>Khanuja, Suman P S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Natural product communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Osman, Magda Abbaker</au><au>Dhawan, Sunita Singh</au><au>Bahl, Janak Raj</au><au>Darokar, Mahendra P</au><au>Khanuja, Suman P S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AFLP Marking and Polymorphism among Progenies of Gymnema sylvestre: an Important Medicinal Plant of India</atitle><jtitle>Natural product communications</jtitle><addtitle>Nat Prod Commun</addtitle><date>2011-11</date><risdate>2011</risdate><volume>6</volume><issue>11</issue><spage>1679</spage><epage>1682</epage><pages>1679-1682</pages><issn>1934-578X</issn><eissn>1555-9475</eissn><abstract>The level of polymorphism among twelve selected progenies of Gymnema sylvestre was investigated through AFLP markers by multiplexing PCR reactions using 64 (8×8) primer combinations. Fourteen primer combinations were selected as the most suitable combination for G. sylvestre. Analysis of the 12 progenies with these 14 primer pairs produced 1689 fragments of which 972 (57.5%) were polymorphic and 485 (28.7%) were unique to a particular genotype. The number of fragments produced by individual primer pairs was in the range of 55 to 225. Out of these, polymorphic fragments were in the range of 34 (E-ACC/M-CAC) to 157 (E-AGG/M-CAG) and unique bands observed were 8 (E-ACC / M-CAC) to 69 (E-AGG/M-CAC). Different primer combinations detected different levels of polymorphism, ranging from 33% (E-AGG/ M-CAC) to 69.8% (E-AGG/ M-CAC). From the observations, it appears that the primer combinations E-AGG/M-CAC, E-AGG/CTG, E-AGG/CAG and E-ACA / CAT were the most informative for the detection of polymorphism among the progenies compared with others, since they produced a high number of unique fragments. The similarity coefficient ranged from 0.212 to 0.731. High similarity was observed between progeny S8 and S9 (73%) and high divergence between progenies S3 and S11. Among the selected progeny, S9 was found to be the most similar to the parent (63%), while genotype S11 was the most distant (36.9%).</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>22224288</pmid><doi>10.1177/1934578X1100601129</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amplified Fragment Length Polymorphism Analysis Cluster Analysis DNA Primers Gymnema sylvestre - genetics India Plants, Medicinal - genetics Polymorphism, Genetic |
| title | AFLP Marking and Polymorphism among Progenies of Gymnema sylvestre: an Important Medicinal Plant of India |
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