Multiclass analysis of 23 veterinary drugs in milk by ultraperformance liquid chromatography–electrospray tandem mass spectrometry
► UPLC–MS/MS method for the simultaneous detection and confirmation veterinary drugs in milk. ► Rapid tool for confirming the presence of 23 veterinary drug residues in milk. ► Extracting more than 30 samples in less than 1 h by using the proposed method. An ultraperformance liquid chromatography–el...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2012-01, Vol.881 (15), p.12-19 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Tang, Yu-Yun Lu, Hsin-Fang Lin, Hsu-Yang Shih, Yang-Chih Hwang, Deng-Fwu |
| description | ► UPLC–MS/MS method for the simultaneous detection and confirmation veterinary drugs in milk. ► Rapid tool for confirming the presence of 23 veterinary drug residues in milk. ► Extracting more than 30 samples in less than 1
h by using the proposed method.
An ultraperformance liquid chromatography–electrospray tandem mass spectrometry (UPLC–MS/MS) method for the simultaneous detection and confirmation of 23 veterinary (multiclass) drugs in milk was developed and validated. The analytes were extracted by acetonitrile, evaporated and injected into the UPLC–MS/MS system on a Waters UPLC HSS T3 column in gradient mode. Data acquisition under MS/MS was achieved by applying multiple reaction monitoring (MRM) of two ion transitions per compound to provide a high degree of specificity. Results showed good repeatability, and recoveries for the 12 macrolide, 7 β-lactam and 2 lincosamide antibiotics and 2 other veterinary drugs (morantel, orbifloxacin) used in milk averaged 51.8–139.0%, 51.5–100.6%, 82.4–102.5% and 87.5–99.4%, respectively. The coefficients of variation (C.V.) of the recoveries were less than 15% for intraday and interday precisions. The limits of quantification (LOQs) were all lower than 5
ng/ml. This method was applied to 17 fresh milk samples and only lincomycin was found in milk samples under allowable levels. Overall, this method is a suitable and rapid tool to confirm the presence of 23 veterinary drug residues in milk. |
| doi_str_mv | 10.1016/j.jchromb.2011.11.005 |
| format | Article |
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h by using the proposed method.
An ultraperformance liquid chromatography–electrospray tandem mass spectrometry (UPLC–MS/MS) method for the simultaneous detection and confirmation of 23 veterinary (multiclass) drugs in milk was developed and validated. The analytes were extracted by acetonitrile, evaporated and injected into the UPLC–MS/MS system on a Waters UPLC HSS T3 column in gradient mode. Data acquisition under MS/MS was achieved by applying multiple reaction monitoring (MRM) of two ion transitions per compound to provide a high degree of specificity. Results showed good repeatability, and recoveries for the 12 macrolide, 7 β-lactam and 2 lincosamide antibiotics and 2 other veterinary drugs (morantel, orbifloxacin) used in milk averaged 51.8–139.0%, 51.5–100.6%, 82.4–102.5% and 87.5–99.4%, respectively. The coefficients of variation (C.V.) of the recoveries were less than 15% for intraday and interday precisions. The limits of quantification (LOQs) were all lower than 5
ng/ml. This method was applied to 17 fresh milk samples and only lincomycin was found in milk samples under allowable levels. Overall, this method is a suitable and rapid tool to confirm the presence of 23 veterinary drug residues in milk.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2011.11.005</identifier><identifier>PMID: 22204873</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>acetonitrile ; Analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Anti-Infective Agents - analysis ; Antibiotics ; beta-lactams ; Biological and medical sciences ; Chromatography ; Chromatography, High Pressure Liquid - methods ; drug residues ; Drug Residues - analysis ; Drugs ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; lincomycin ; Liquids ; macrolides ; Mass spectrometry ; Medical sciences ; Milk ; Milk - chemistry ; Monitoring ; morantel ; Multiclass analysis ; Pharmacology. Drug treatments ; Recovery ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrometry, Mass, Electrospray Ionization - methods ; Tandem mass spectrometry ; Tandem Mass Spectrometry - methods ; Veterinary ; veterinary drugs ; Veterinary Drugs - analysis</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2012-01, Vol.881 (15), p.12-19</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-a3c1cf60d46565b2a804bf56f2ad02314d02149c9a71e17ded164910e3882b8e3</citedby><cites>FETCH-LOGICAL-c451t-a3c1cf60d46565b2a804bf56f2ad02314d02149c9a71e17ded164910e3882b8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2011.11.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25395451$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22204873$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Yu-Yun</creatorcontrib><creatorcontrib>Lu, Hsin-Fang</creatorcontrib><creatorcontrib>Lin, Hsu-Yang</creatorcontrib><creatorcontrib>Shih, Yang-Chih</creatorcontrib><creatorcontrib>Hwang, Deng-Fwu</creatorcontrib><title>Multiclass analysis of 23 veterinary drugs in milk by ultraperformance liquid chromatography–electrospray tandem mass spectrometry</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>► UPLC–MS/MS method for the simultaneous detection and confirmation veterinary drugs in milk. ► Rapid tool for confirming the presence of 23 veterinary drug residues in milk. ► Extracting more than 30 samples in less than 1
h by using the proposed method.
An ultraperformance liquid chromatography–electrospray tandem mass spectrometry (UPLC–MS/MS) method for the simultaneous detection and confirmation of 23 veterinary (multiclass) drugs in milk was developed and validated. The analytes were extracted by acetonitrile, evaporated and injected into the UPLC–MS/MS system on a Waters UPLC HSS T3 column in gradient mode. Data acquisition under MS/MS was achieved by applying multiple reaction monitoring (MRM) of two ion transitions per compound to provide a high degree of specificity. Results showed good repeatability, and recoveries for the 12 macrolide, 7 β-lactam and 2 lincosamide antibiotics and 2 other veterinary drugs (morantel, orbifloxacin) used in milk averaged 51.8–139.0%, 51.5–100.6%, 82.4–102.5% and 87.5–99.4%, respectively. The coefficients of variation (C.V.) of the recoveries were less than 15% for intraday and interday precisions. The limits of quantification (LOQs) were all lower than 5
ng/ml. This method was applied to 17 fresh milk samples and only lincomycin was found in milk samples under allowable levels. Overall, this method is a suitable and rapid tool to confirm the presence of 23 veterinary drug residues in milk.</description><subject>acetonitrile</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Anti-Infective Agents - analysis</subject><subject>Antibiotics</subject><subject>beta-lactams</subject><subject>Biological and medical sciences</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>drug residues</subject><subject>Drug Residues - analysis</subject><subject>Drugs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>lincomycin</subject><subject>Liquids</subject><subject>macrolides</subject><subject>Mass spectrometry</subject><subject>Medical sciences</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>Monitoring</subject><subject>morantel</subject><subject>Multiclass analysis</subject><subject>Pharmacology. Drug treatments</subject><subject>Recovery</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Tandem mass spectrometry</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Veterinary</subject><subject>veterinary drugs</subject><subject>Veterinary Drugs - analysis</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhiMEoqXwCIA3CDYZfE-yqlDFTSpiAZXYWY59MvXgJFM7qZQdi75B35An4UxngB1Ilm3pfOf2_0XxlNEVo0y_3qw27jKNfbvilLEVHkrVveKY1ZUoRaW_3ce_qmhJueBHxaOcN5SyilbiYXHEOacSwePi5tMcp-CizZnYwcYlh0zGjnBBrmGCFAabFuLTvM4kDKQP8TtpF4JJyW4hdWPq7eCAxHA1B0_uRrLTuMbo5fLzxy1EcFMa8zbZhUx28NCTftcsb-8CPUxpeVw86GzM8OTwnhQX795-PftQnn9-__HszXnppGJTaYVjrtPUS620armtqWw7pTtuPW7JJN5MNq6xFQNWefBMy4ZREHXN2xrESfFyX3ebxqsZ8mT6kB3EaAcY52wapqholBJIvvoniRbQupFNJRFVe9ThmjlBZ7Yp9KgaQjtOm405WGV2Vhk8aBXmPTu0mNse_J-s394g8OIA2Oxs7BIKHfJfTuGoqAtyz_dcZ0dj1wmZiy_YSVNKdcXlrtLpngAU9zpAMtkFQNt8SOiC8WP4z7C_AC_twVI</recordid><startdate>20120115</startdate><enddate>20120115</enddate><creator>Tang, Yu-Yun</creator><creator>Lu, Hsin-Fang</creator><creator>Lin, Hsu-Yang</creator><creator>Shih, Yang-Chih</creator><creator>Hwang, Deng-Fwu</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>7X8</scope></search><sort><creationdate>20120115</creationdate><title>Multiclass analysis of 23 veterinary drugs in milk by ultraperformance liquid chromatography–electrospray tandem mass spectrometry</title><author>Tang, Yu-Yun ; Lu, Hsin-Fang ; Lin, Hsu-Yang ; Shih, Yang-Chih ; Hwang, Deng-Fwu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-a3c1cf60d46565b2a804bf56f2ad02314d02149c9a71e17ded164910e3882b8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>acetonitrile</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Anti-Infective Agents - analysis</topic><topic>Antibiotics</topic><topic>beta-lactams</topic><topic>Biological and medical sciences</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>drug residues</topic><topic>Drug Residues - analysis</topic><topic>Drugs</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>lincomycin</topic><topic>Liquids</topic><topic>macrolides</topic><topic>Mass spectrometry</topic><topic>Medical sciences</topic><topic>Milk</topic><topic>Milk - chemistry</topic><topic>Monitoring</topic><topic>morantel</topic><topic>Multiclass analysis</topic><topic>Pharmacology. Drug treatments</topic><topic>Recovery</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Tandem mass spectrometry</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Veterinary</topic><topic>veterinary drugs</topic><topic>Veterinary Drugs - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Yu-Yun</creatorcontrib><creatorcontrib>Lu, Hsin-Fang</creatorcontrib><creatorcontrib>Lin, Hsu-Yang</creatorcontrib><creatorcontrib>Shih, Yang-Chih</creatorcontrib><creatorcontrib>Hwang, Deng-Fwu</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Yu-Yun</au><au>Lu, Hsin-Fang</au><au>Lin, Hsu-Yang</au><au>Shih, Yang-Chih</au><au>Hwang, Deng-Fwu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiclass analysis of 23 veterinary drugs in milk by ultraperformance liquid chromatography–electrospray tandem mass spectrometry</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2012-01-15</date><risdate>2012</risdate><volume>881</volume><issue>15</issue><spage>12</spage><epage>19</epage><pages>12-19</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>► UPLC–MS/MS method for the simultaneous detection and confirmation veterinary drugs in milk. ► Rapid tool for confirming the presence of 23 veterinary drug residues in milk. ► Extracting more than 30 samples in less than 1
h by using the proposed method.
An ultraperformance liquid chromatography–electrospray tandem mass spectrometry (UPLC–MS/MS) method for the simultaneous detection and confirmation of 23 veterinary (multiclass) drugs in milk was developed and validated. The analytes were extracted by acetonitrile, evaporated and injected into the UPLC–MS/MS system on a Waters UPLC HSS T3 column in gradient mode. Data acquisition under MS/MS was achieved by applying multiple reaction monitoring (MRM) of two ion transitions per compound to provide a high degree of specificity. Results showed good repeatability, and recoveries for the 12 macrolide, 7 β-lactam and 2 lincosamide antibiotics and 2 other veterinary drugs (morantel, orbifloxacin) used in milk averaged 51.8–139.0%, 51.5–100.6%, 82.4–102.5% and 87.5–99.4%, respectively. The coefficients of variation (C.V.) of the recoveries were less than 15% for intraday and interday precisions. The limits of quantification (LOQs) were all lower than 5
ng/ml. This method was applied to 17 fresh milk samples and only lincomycin was found in milk samples under allowable levels. Overall, this method is a suitable and rapid tool to confirm the presence of 23 veterinary drug residues in milk.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22204873</pmid><doi>10.1016/j.jchromb.2011.11.005</doi><tpages>8</tpages></addata></record> |
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| subjects | acetonitrile Analysis Analytical, structural and metabolic biochemistry Animals Anti-Infective Agents - analysis Antibiotics beta-lactams Biological and medical sciences Chromatography Chromatography, High Pressure Liquid - methods drug residues Drug Residues - analysis Drugs Fundamental and applied biological sciences. Psychology General pharmacology lincomycin Liquids macrolides Mass spectrometry Medical sciences Milk Milk - chemistry Monitoring morantel Multiclass analysis Pharmacology. Drug treatments Recovery Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods Tandem mass spectrometry Tandem Mass Spectrometry - methods Veterinary veterinary drugs Veterinary Drugs - analysis |
| title | Multiclass analysis of 23 veterinary drugs in milk by ultraperformance liquid chromatography–electrospray tandem mass spectrometry |
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