Effects of discontinuous Percoll gradient centrifugation on the quality of bovine spermatozoa evaluated with computer-assisted semen analysis and fluorescent probes association

Summary The objective of this study was to evaluate the quality of bovine frozen‐thawed sperm cells after Percoll gradient centrifugation. Frozen semen doses were obtained from six bulls of different breeds, including three taurine and three Zebu animals. Four ejaculates per bull were evaluated befo...

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Veröffentlicht in:	Andrologia 2012-02, Vol.44 (1), p.9-15
Hauptverfasser:	Oliveira, L. Z., Arruda, R. P., Celeghini, E. C. C., de Andrade, A. F. C., Perini, A. P., Resende, M. V., Miguel, M. C. V., Lucio, A. C., Hossepian de Lima, V. F. M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrosome integrity Animals Cattle Cell Separation - veterinary Centrifugation, Density Gradient Computers Cryopreservation - veterinary cryopreserved semen Fluorescent Dyes Male Povidone Semen Analysis - veterinary Semen Preservation - veterinary Silicon Dioxide Sperm Motility sperm selection Spermatozoa - physiology
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