In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn
ABSTRACT A very simple and direct method has been established for the determination of polygalic acid and its metabolites in rat urine based on HPLC coupled with electrospray ionization multi‐stage tandem mass spectrometry (HPLC‐ESI‐MSn). The rats were administered a single dose (100 mg/kg) of polyg...
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| Veröffentlicht in: | Biomedical chromatography 2012-02, Vol.26 (2), p.220-224 |
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| creator | Ling, Yun Wu, Bin Wang, Ke Liu, Huihui Chen, Luying Fan, Mingsong Sun, Zhaolin Huang, Chenggang |
| description | ABSTRACT
A very simple and direct method has been established for the determination of polygalic acid and its metabolites in rat urine based on HPLC coupled with electrospray ionization multi‐stage tandem mass spectrometry (HPLC‐ESI‐MSn). The rats were administered a single dose (100 mg/kg) of polygalic acid by oral gavage. The urine samples were collected and purified through a C18 solid‐phase extraction cartridge, and then these pretreated samples were injected into a reversed‐phase C18 column with a gradient elution program, whereas acetonitrile–0.5% aqueous formic acid was used as mobile phase and detected by an on‐line MS/MS system. As a result, the parent drug and its four metabolites were identified and characterized in rat urine for the first time by comparing their changes in molecular mass (ΔM), retention times and full‐scan MSn spectra with those of the parent drug. A possible metabolic pathway of polygalic acid was investigated and proposed. More importantly, the results demonstrated that the newly developed method (HPLC‐ESI‐MSn) was sensitive, simple and suitable for the determination of polygalic acid and its metabolites in biological samples. Copyright © 2011 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/bmc.1650 |
| format | Article |
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A very simple and direct method has been established for the determination of polygalic acid and its metabolites in rat urine based on HPLC coupled with electrospray ionization multi‐stage tandem mass spectrometry (HPLC‐ESI‐MSn). The rats were administered a single dose (100 mg/kg) of polygalic acid by oral gavage. The urine samples were collected and purified through a C18 solid‐phase extraction cartridge, and then these pretreated samples were injected into a reversed‐phase C18 column with a gradient elution program, whereas acetonitrile–0.5% aqueous formic acid was used as mobile phase and detected by an on‐line MS/MS system. As a result, the parent drug and its four metabolites were identified and characterized in rat urine for the first time by comparing their changes in molecular mass (ΔM), retention times and full‐scan MSn spectra with those of the parent drug. A possible metabolic pathway of polygalic acid was investigated and proposed. More importantly, the results demonstrated that the newly developed method (HPLC‐ESI‐MSn) was sensitive, simple and suitable for the determination of polygalic acid and its metabolites in biological samples. Copyright © 2011 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.1650</identifier><identifier>PMID: 21618563</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Chromatography, High Pressure Liquid - methods ; Gallic Acid - metabolism ; Gallic Acid - urine ; Glucuronic Acid - metabolism ; LC-ESI-MSn ; metabolite ; Plant Extracts - chemistry ; Plant Extracts - metabolism ; Plant Extracts - urine ; Polygala - chemistry ; polygalic acid ; rat ; Rats ; Rats, Sprague-Dawley ; Spectrometry, Mass, Electrospray Ionization - methods ; Tandem Mass Spectrometry - methods ; urine</subject><ispartof>Biomedical chromatography, 2012-02, Vol.26 (2), p.220-224</ispartof><rights>Copyright © 2011 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.1650$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.1650$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21618563$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ling, Yun</creatorcontrib><creatorcontrib>Wu, Bin</creatorcontrib><creatorcontrib>Wang, Ke</creatorcontrib><creatorcontrib>Liu, Huihui</creatorcontrib><creatorcontrib>Chen, Luying</creatorcontrib><creatorcontrib>Fan, Mingsong</creatorcontrib><creatorcontrib>Sun, Zhaolin</creatorcontrib><creatorcontrib>Huang, Chenggang</creatorcontrib><title>In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>ABSTRACT
A very simple and direct method has been established for the determination of polygalic acid and its metabolites in rat urine based on HPLC coupled with electrospray ionization multi‐stage tandem mass spectrometry (HPLC‐ESI‐MSn). The rats were administered a single dose (100 mg/kg) of polygalic acid by oral gavage. The urine samples were collected and purified through a C18 solid‐phase extraction cartridge, and then these pretreated samples were injected into a reversed‐phase C18 column with a gradient elution program, whereas acetonitrile–0.5% aqueous formic acid was used as mobile phase and detected by an on‐line MS/MS system. As a result, the parent drug and its four metabolites were identified and characterized in rat urine for the first time by comparing their changes in molecular mass (ΔM), retention times and full‐scan MSn spectra with those of the parent drug. A possible metabolic pathway of polygalic acid was investigated and proposed. More importantly, the results demonstrated that the newly developed method (HPLC‐ESI‐MSn) was sensitive, simple and suitable for the determination of polygalic acid and its metabolites in biological samples. Copyright © 2011 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Gallic Acid - metabolism</subject><subject>Gallic Acid - urine</subject><subject>Glucuronic Acid - metabolism</subject><subject>LC-ESI-MSn</subject><subject>metabolite</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - metabolism</subject><subject>Plant Extracts - urine</subject><subject>Polygala - chemistry</subject><subject>polygalic acid</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>urine</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtOwzAQRS0EoqUg8QXIO1Yp4zi2kyWE0hZaQBQEO8tJHGTIo8RJIX9Pqpayuos5ujNzEDolMCQA7kWUx0PCGeyhPoEgcMAHso_64PLAob4IeujI2g8ACLgrDlHPJZz4jNM-up4WeGVWJc51raIyMzbHtm6SFpcpXpZZ-64yE2MVmwSbAleqxo01xTuePM5CZ7SYOvNFcYwOUpVZfbLNAXq5GT2HE2f2MJ6GlzPHdAvBETxNwXdd8EBzlzEWcUFioFGimCCMRlzTJKYei7qTA6EipYSfJIRoIZSnYzpA55veZVV-NdrWMjc21lmmCl02VgbE45z7nHXk2ZZsolwnclmZXFWt_Hu8A5wN8G0y3e7mBORaqOyEyrVQeTUP1_nPG1vrnx2vqk_JBRVMvt6PJbzdPnEx9-Ud_QWlHXQN</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Ling, Yun</creator><creator>Wu, Bin</creator><creator>Wang, Ke</creator><creator>Liu, Huihui</creator><creator>Chen, Luying</creator><creator>Fan, Mingsong</creator><creator>Sun, Zhaolin</creator><creator>Huang, Chenggang</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201202</creationdate><title>In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn</title><author>Ling, Yun ; Wu, Bin ; Wang, Ke ; Liu, Huihui ; Chen, Luying ; Fan, Mingsong ; Sun, Zhaolin ; Huang, Chenggang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2160-76ff0822040e62555b671c03bda57153b6e3dc345b87997abaa78dd11e77a4ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Gallic Acid - metabolism</topic><topic>Gallic Acid - urine</topic><topic>Glucuronic Acid - metabolism</topic><topic>LC-ESI-MSn</topic><topic>metabolite</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - metabolism</topic><topic>Plant Extracts - urine</topic><topic>Polygala - chemistry</topic><topic>polygalic acid</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ling, Yun</creatorcontrib><creatorcontrib>Wu, Bin</creatorcontrib><creatorcontrib>Wang, Ke</creatorcontrib><creatorcontrib>Liu, Huihui</creatorcontrib><creatorcontrib>Chen, Luying</creatorcontrib><creatorcontrib>Fan, Mingsong</creatorcontrib><creatorcontrib>Sun, Zhaolin</creatorcontrib><creatorcontrib>Huang, Chenggang</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ling, Yun</au><au>Wu, Bin</au><au>Wang, Ke</au><au>Liu, Huihui</au><au>Chen, Luying</au><au>Fan, Mingsong</au><au>Sun, Zhaolin</au><au>Huang, Chenggang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>2012-02</date><risdate>2012</risdate><volume>26</volume><issue>2</issue><spage>220</spage><epage>224</epage><pages>220-224</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>ABSTRACT
A very simple and direct method has been established for the determination of polygalic acid and its metabolites in rat urine based on HPLC coupled with electrospray ionization multi‐stage tandem mass spectrometry (HPLC‐ESI‐MSn). The rats were administered a single dose (100 mg/kg) of polygalic acid by oral gavage. The urine samples were collected and purified through a C18 solid‐phase extraction cartridge, and then these pretreated samples were injected into a reversed‐phase C18 column with a gradient elution program, whereas acetonitrile–0.5% aqueous formic acid was used as mobile phase and detected by an on‐line MS/MS system. As a result, the parent drug and its four metabolites were identified and characterized in rat urine for the first time by comparing their changes in molecular mass (ΔM), retention times and full‐scan MSn spectra with those of the parent drug. A possible metabolic pathway of polygalic acid was investigated and proposed. More importantly, the results demonstrated that the newly developed method (HPLC‐ESI‐MSn) was sensitive, simple and suitable for the determination of polygalic acid and its metabolites in biological samples. Copyright © 2011 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>21618563</pmid><doi>10.1002/bmc.1650</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Chromatography, High Pressure Liquid - methods Gallic Acid - metabolism Gallic Acid - urine Glucuronic Acid - metabolism LC-ESI-MSn metabolite Plant Extracts - chemistry Plant Extracts - metabolism Plant Extracts - urine Polygala - chemistry polygalic acid rat Rats Rats, Sprague-Dawley Spectrometry, Mass, Electrospray Ionization - methods Tandem Mass Spectrometry - methods urine |
| title | In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn |
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