Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles
This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by...
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Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2011-09, Vol.157 (1), p.189-194 |
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creator | Li, Li-Dong Zhao, Hong-Tao Chen, Zheng-Bo Mu, Xiao-Jiao Guo, Lin |
description | This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by using 1,6-Hexanedithiol as a medium. To quantify the amount of thrombin, changes in the interfacial electron transfer resistance (
R
et) of the aptasensor were monitored using the redox couple of an [Fe(CN)
6]
3−/4− probe. The plot of
(
R
e
t
i
−
R
e
t
0
)
/
R
e
t
0
against the logarithm of thrombin concentration is linear with over the range from 0.1
nM to 30
nM with a detection limit of 0.013
nM. Meanwhile, the packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH
3)
6]
3+) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode. The aptasensor also showed good selectivity for thrombin without being affected by the presence of other proteins. |
doi_str_mv | 10.1016/j.snb.2011.03.048 |
format | Article |
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R
et) of the aptasensor were monitored using the redox couple of an [Fe(CN)
6]
3−/4− probe. The plot of
(
R
e
t
i
−
R
e
t
0
)
/
R
e
t
0
against the logarithm of thrombin concentration is linear with over the range from 0.1
nM to 30
nM with a detection limit of 0.013
nM. Meanwhile, the packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH
3)
6]
3+) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode. The aptasensor also showed good selectivity for thrombin without being affected by the presence of other proteins.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2011.03.048</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>ammonia ; Aptamer ; biosensors ; cations ; Cyclic voltammetry (CV) ; detection limit ; dielectric spectroscopy ; DNA ; Electrochemical impedance spectroscopy (EIS) ; electrodes ; electron transfer ; Gold nanoparticles ; iron ; nanogold ; oligonucleotides ; Thrombin</subject><ispartof>Sensors and actuators. B, Chemical, 2011-09, Vol.157 (1), p.189-194</ispartof><rights>2011 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-984890806f416d1b747987c14278eb127855daeed1e3d414cb7942b39b7159ee3</citedby><cites>FETCH-LOGICAL-c353t-984890806f416d1b747987c14278eb127855daeed1e3d414cb7942b39b7159ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.snb.2011.03.048$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Li, Li-Dong</creatorcontrib><creatorcontrib>Zhao, Hong-Tao</creatorcontrib><creatorcontrib>Chen, Zheng-Bo</creatorcontrib><creatorcontrib>Mu, Xiao-Jiao</creatorcontrib><creatorcontrib>Guo, Lin</creatorcontrib><title>Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles</title><title>Sensors and actuators. B, Chemical</title><description>This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by using 1,6-Hexanedithiol as a medium. To quantify the amount of thrombin, changes in the interfacial electron transfer resistance (
R
et) of the aptasensor were monitored using the redox couple of an [Fe(CN)
6]
3−/4− probe. The plot of
(
R
e
t
i
−
R
e
t
0
)
/
R
e
t
0
against the logarithm of thrombin concentration is linear with over the range from 0.1
nM to 30
nM with a detection limit of 0.013
nM. Meanwhile, the packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH
3)
6]
3+) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode. The aptasensor also showed good selectivity for thrombin without being affected by the presence of other proteins.</description><subject>ammonia</subject><subject>Aptamer</subject><subject>biosensors</subject><subject>cations</subject><subject>Cyclic voltammetry (CV)</subject><subject>detection limit</subject><subject>dielectric spectroscopy</subject><subject>DNA</subject><subject>Electrochemical impedance spectroscopy (EIS)</subject><subject>electrodes</subject><subject>electron transfer</subject><subject>Gold nanoparticles</subject><subject>iron</subject><subject>nanogold</subject><subject>oligonucleotides</subject><subject>Thrombin</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kM9r3TAMgM3oYG_d_oCd6ttOSaVnJ47ZqZS2GxR62Ho2_qF0fiRxaqeF_vfz4-3cgyQkPgn0MfYNoUXA_vLQlsW1e0BsQbQghw9sh4MSjQClztgO9L5rJED3iX0u5QAAUvSwY_Fq3exMmbuYCi0lZT7WmKyjqRkzEY_zSsEunnhZyW85FZ_WNx5oq11MC08j3_7mNLu4cGcLBV6HT2kKfLFLWm3eop-ofGEfRzsV-vq_nrPH25s_1z-b-4e7X9dX940XndgaPchBwwD9KLEP6JRUelAe5V4N5LDmrguWKCCJIFF6p7TcO6Gdwk4TiXP2_XR3zen5hcpm5lg8TZNdKL0Uo1H2OHQoK4kn0tenSqbRrDnONr8ZBHO0ag6mWjVHqwaEqVbrzsVpZ7TJ2Kcci3n8XYEOKqS0PhI_TgTVJ18jZVN8pOovxFyNmZDiO_f_Aez5igc</recordid><startdate>20110920</startdate><enddate>20110920</enddate><creator>Li, Li-Dong</creator><creator>Zhao, Hong-Tao</creator><creator>Chen, Zheng-Bo</creator><creator>Mu, Xiao-Jiao</creator><creator>Guo, Lin</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110920</creationdate><title>Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles</title><author>Li, Li-Dong ; Zhao, Hong-Tao ; Chen, Zheng-Bo ; Mu, Xiao-Jiao ; Guo, Lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-984890806f416d1b747987c14278eb127855daeed1e3d414cb7942b39b7159ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>ammonia</topic><topic>Aptamer</topic><topic>biosensors</topic><topic>cations</topic><topic>Cyclic voltammetry (CV)</topic><topic>detection limit</topic><topic>dielectric spectroscopy</topic><topic>DNA</topic><topic>Electrochemical impedance spectroscopy (EIS)</topic><topic>electrodes</topic><topic>electron transfer</topic><topic>Gold nanoparticles</topic><topic>iron</topic><topic>nanogold</topic><topic>oligonucleotides</topic><topic>Thrombin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Li-Dong</creatorcontrib><creatorcontrib>Zhao, Hong-Tao</creatorcontrib><creatorcontrib>Chen, Zheng-Bo</creatorcontrib><creatorcontrib>Mu, Xiao-Jiao</creatorcontrib><creatorcontrib>Guo, Lin</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Li-Dong</au><au>Zhao, Hong-Tao</au><au>Chen, Zheng-Bo</au><au>Mu, Xiao-Jiao</au><au>Guo, Lin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2011-09-20</date><risdate>2011</risdate><volume>157</volume><issue>1</issue><spage>189</spage><epage>194</epage><pages>189-194</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by using 1,6-Hexanedithiol as a medium. To quantify the amount of thrombin, changes in the interfacial electron transfer resistance (
R
et) of the aptasensor were monitored using the redox couple of an [Fe(CN)
6]
3−/4− probe. The plot of
(
R
e
t
i
−
R
e
t
0
)
/
R
e
t
0
against the logarithm of thrombin concentration is linear with over the range from 0.1
nM to 30
nM with a detection limit of 0.013
nM. Meanwhile, the packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH
3)
6]
3+) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode. The aptasensor also showed good selectivity for thrombin without being affected by the presence of other proteins.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2011.03.048</doi><tpages>6</tpages></addata></record> |
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language | eng |
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source | ScienceDirect Journals (5 years ago - present) |
subjects | ammonia Aptamer biosensors cations Cyclic voltammetry (CV) detection limit dielectric spectroscopy DNA Electrochemical impedance spectroscopy (EIS) electrodes electron transfer Gold nanoparticles iron nanogold oligonucleotides Thrombin |
title | Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles |
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