Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles

This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Sensors and actuators. B, Chemical Chemical, 2011-09, Vol.157 (1), p.189-194
Hauptverfasser: Li, Li-Dong, Zhao, Hong-Tao, Chen, Zheng-Bo, Mu, Xiao-Jiao, Guo, Lin
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 194
container_issue 1
container_start_page 189
container_title Sensors and actuators. B, Chemical
container_volume 157
creator Li, Li-Dong
Zhao, Hong-Tao
Chen, Zheng-Bo
Mu, Xiao-Jiao
Guo, Lin
description This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by using 1,6-Hexanedithiol as a medium. To quantify the amount of thrombin, changes in the interfacial electron transfer resistance ( R et) of the aptasensor were monitored using the redox couple of an [Fe(CN) 6] 3−/4− probe. The plot of ( R e t i − R e t 0 ) / R e t 0 against the logarithm of thrombin concentration is linear with over the range from 0.1 nM to 30 nM with a detection limit of 0.013 nM. Meanwhile, the packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH 3) 6] 3+) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode. The aptasensor also showed good selectivity for thrombin without being affected by the presence of other proteins.
doi_str_mv 10.1016/j.snb.2011.03.048
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_914618514</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0925400511002449</els_id><sourcerecordid>914618514</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-984890806f416d1b747987c14278eb127855daeed1e3d414cb7942b39b7159ee3</originalsourceid><addsrcrecordid>eNp9kM9r3TAMgM3oYG_d_oCd6ttOSaVnJ47ZqZS2GxR62Ho2_qF0fiRxaqeF_vfz4-3cgyQkPgn0MfYNoUXA_vLQlsW1e0BsQbQghw9sh4MSjQClztgO9L5rJED3iX0u5QAAUvSwY_Fq3exMmbuYCi0lZT7WmKyjqRkzEY_zSsEunnhZyW85FZ_WNx5oq11MC08j3_7mNLu4cGcLBV6HT2kKfLFLWm3eop-ofGEfRzsV-vq_nrPH25s_1z-b-4e7X9dX940XndgaPchBwwD9KLEP6JRUelAe5V4N5LDmrguWKCCJIFF6p7TcO6Gdwk4TiXP2_XR3zen5hcpm5lg8TZNdKL0Uo1H2OHQoK4kn0tenSqbRrDnONr8ZBHO0ag6mWjVHqwaEqVbrzsVpZ7TJ2Kcci3n8XYEOKqS0PhI_TgTVJ18jZVN8pOovxFyNmZDiO_f_Aez5igc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>914618514</pqid></control><display><type>article</type><title>Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles</title><source>ScienceDirect Journals (5 years ago - present)</source><creator>Li, Li-Dong ; Zhao, Hong-Tao ; Chen, Zheng-Bo ; Mu, Xiao-Jiao ; Guo, Lin</creator><creatorcontrib>Li, Li-Dong ; Zhao, Hong-Tao ; Chen, Zheng-Bo ; Mu, Xiao-Jiao ; Guo, Lin</creatorcontrib><description>This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by using 1,6-Hexanedithiol as a medium. To quantify the amount of thrombin, changes in the interfacial electron transfer resistance ( R et) of the aptasensor were monitored using the redox couple of an [Fe(CN) 6] 3−/4− probe. The plot of ( R e t i − R e t 0 ) / R e t 0 against the logarithm of thrombin concentration is linear with over the range from 0.1 nM to 30 nM with a detection limit of 0.013 nM. Meanwhile, the packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH 3) 6] 3+) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode. The aptasensor also showed good selectivity for thrombin without being affected by the presence of other proteins.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2011.03.048</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>ammonia ; Aptamer ; biosensors ; cations ; Cyclic voltammetry (CV) ; detection limit ; dielectric spectroscopy ; DNA ; Electrochemical impedance spectroscopy (EIS) ; electrodes ; electron transfer ; Gold nanoparticles ; iron ; nanogold ; oligonucleotides ; Thrombin</subject><ispartof>Sensors and actuators. B, Chemical, 2011-09, Vol.157 (1), p.189-194</ispartof><rights>2011 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-984890806f416d1b747987c14278eb127855daeed1e3d414cb7942b39b7159ee3</citedby><cites>FETCH-LOGICAL-c353t-984890806f416d1b747987c14278eb127855daeed1e3d414cb7942b39b7159ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.snb.2011.03.048$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Li, Li-Dong</creatorcontrib><creatorcontrib>Zhao, Hong-Tao</creatorcontrib><creatorcontrib>Chen, Zheng-Bo</creatorcontrib><creatorcontrib>Mu, Xiao-Jiao</creatorcontrib><creatorcontrib>Guo, Lin</creatorcontrib><title>Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles</title><title>Sensors and actuators. B, Chemical</title><description>This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by using 1,6-Hexanedithiol as a medium. To quantify the amount of thrombin, changes in the interfacial electron transfer resistance ( R et) of the aptasensor were monitored using the redox couple of an [Fe(CN) 6] 3−/4− probe. The plot of ( R e t i − R e t 0 ) / R e t 0 against the logarithm of thrombin concentration is linear with over the range from 0.1 nM to 30 nM with a detection limit of 0.013 nM. Meanwhile, the packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH 3) 6] 3+) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode. The aptasensor also showed good selectivity for thrombin without being affected by the presence of other proteins.</description><subject>ammonia</subject><subject>Aptamer</subject><subject>biosensors</subject><subject>cations</subject><subject>Cyclic voltammetry (CV)</subject><subject>detection limit</subject><subject>dielectric spectroscopy</subject><subject>DNA</subject><subject>Electrochemical impedance spectroscopy (EIS)</subject><subject>electrodes</subject><subject>electron transfer</subject><subject>Gold nanoparticles</subject><subject>iron</subject><subject>nanogold</subject><subject>oligonucleotides</subject><subject>Thrombin</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kM9r3TAMgM3oYG_d_oCd6ttOSaVnJ47ZqZS2GxR62Ho2_qF0fiRxaqeF_vfz4-3cgyQkPgn0MfYNoUXA_vLQlsW1e0BsQbQghw9sh4MSjQClztgO9L5rJED3iX0u5QAAUvSwY_Fq3exMmbuYCi0lZT7WmKyjqRkzEY_zSsEunnhZyW85FZ_WNx5oq11MC08j3_7mNLu4cGcLBV6HT2kKfLFLWm3eop-ofGEfRzsV-vq_nrPH25s_1z-b-4e7X9dX940XndgaPchBwwD9KLEP6JRUelAe5V4N5LDmrguWKCCJIFF6p7TcO6Gdwk4TiXP2_XR3zen5hcpm5lg8TZNdKL0Uo1H2OHQoK4kn0tenSqbRrDnONr8ZBHO0ag6mWjVHqwaEqVbrzsVpZ7TJ2Kcci3n8XYEOKqS0PhI_TgTVJ18jZVN8pOovxFyNmZDiO_f_Aez5igc</recordid><startdate>20110920</startdate><enddate>20110920</enddate><creator>Li, Li-Dong</creator><creator>Zhao, Hong-Tao</creator><creator>Chen, Zheng-Bo</creator><creator>Mu, Xiao-Jiao</creator><creator>Guo, Lin</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110920</creationdate><title>Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles</title><author>Li, Li-Dong ; Zhao, Hong-Tao ; Chen, Zheng-Bo ; Mu, Xiao-Jiao ; Guo, Lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-984890806f416d1b747987c14278eb127855daeed1e3d414cb7942b39b7159ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>ammonia</topic><topic>Aptamer</topic><topic>biosensors</topic><topic>cations</topic><topic>Cyclic voltammetry (CV)</topic><topic>detection limit</topic><topic>dielectric spectroscopy</topic><topic>DNA</topic><topic>Electrochemical impedance spectroscopy (EIS)</topic><topic>electrodes</topic><topic>electron transfer</topic><topic>Gold nanoparticles</topic><topic>iron</topic><topic>nanogold</topic><topic>oligonucleotides</topic><topic>Thrombin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Li-Dong</creatorcontrib><creatorcontrib>Zhao, Hong-Tao</creatorcontrib><creatorcontrib>Chen, Zheng-Bo</creatorcontrib><creatorcontrib>Mu, Xiao-Jiao</creatorcontrib><creatorcontrib>Guo, Lin</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Li-Dong</au><au>Zhao, Hong-Tao</au><au>Chen, Zheng-Bo</au><au>Mu, Xiao-Jiao</au><au>Guo, Lin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2011-09-20</date><risdate>2011</risdate><volume>157</volume><issue>1</issue><spage>189</spage><epage>194</epage><pages>189-194</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by using 1,6-Hexanedithiol as a medium. To quantify the amount of thrombin, changes in the interfacial electron transfer resistance ( R et) of the aptasensor were monitored using the redox couple of an [Fe(CN) 6] 3−/4− probe. The plot of ( R e t i − R e t 0 ) / R e t 0 against the logarithm of thrombin concentration is linear with over the range from 0.1 nM to 30 nM with a detection limit of 0.013 nM. Meanwhile, the packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH 3) 6] 3+) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode. The aptasensor also showed good selectivity for thrombin without being affected by the presence of other proteins.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2011.03.048</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0925-4005
ispartof Sensors and actuators. B, Chemical, 2011-09, Vol.157 (1), p.189-194
issn 0925-4005
1873-3077
language eng
recordid cdi_proquest_miscellaneous_914618514
source ScienceDirect Journals (5 years ago - present)
subjects ammonia
Aptamer
biosensors
cations
Cyclic voltammetry (CV)
detection limit
dielectric spectroscopy
DNA
Electrochemical impedance spectroscopy (EIS)
electrodes
electron transfer
Gold nanoparticles
iron
nanogold
oligonucleotides
Thrombin
title Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T18%3A54%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Aptamer%20biosensor%20for%20label-free%20impedance%20spectroscopy%20detection%20of%20thrombin%20based%20on%20gold%20nanoparticles&rft.jtitle=Sensors%20and%20actuators.%20B,%20Chemical&rft.au=Li,%20Li-Dong&rft.date=2011-09-20&rft.volume=157&rft.issue=1&rft.spage=189&rft.epage=194&rft.pages=189-194&rft.issn=0925-4005&rft.eissn=1873-3077&rft_id=info:doi/10.1016/j.snb.2011.03.048&rft_dat=%3Cproquest_cross%3E914618514%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=914618514&rft_id=info:pmid/&rft_els_id=S0925400511002449&rfr_iscdi=true