Association of SCD1 and DGAT1 SNPs with the intramuscular fat traits in Chinese Simmental cattle and their distribution in eight Chinese cattle breeds

Intramuscular fat (IMF) is a key parameter for evaluation of nutritional quality of beef, with its endogenous synthesis regulated by stearoyl CoA desaturase ( SCD1 ) and diacylglycerol-acyl transferase 1 ( DGAT1 ) genes in cattle. The object of this research was to evaluate the effect of SCD1 and DG...

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Veröffentlicht in:Molecular biology reports 2012-02, Vol.39 (2), p.1065-1071
Hauptverfasser: Wu, X. X., Yang, Z. P., Shi, X. K., Li, J. Y., Ji, D. J., Mao, Y. J., Chang, L. L., Gao, H. J.
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container_title Molecular biology reports
container_volume 39
creator Wu, X. X.
Yang, Z. P.
Shi, X. K.
Li, J. Y.
Ji, D. J.
Mao, Y. J.
Chang, L. L.
Gao, H. J.
description Intramuscular fat (IMF) is a key parameter for evaluation of nutritional quality of beef, with its endogenous synthesis regulated by stearoyl CoA desaturase ( SCD1 ) and diacylglycerol-acyl transferase 1 ( DGAT1 ) genes in cattle. The object of this research was to evaluate the effect of SCD1 and DGAT1 polymorphisms on IMF trait in beef cattle and to estimate the frequency distribution of SNPs in the two genes in Chinese cattle populations. The SCD1 and DGAT1 polymorphisms were detected by PCR-single strand conformation polymorphism (PCR-SSCP) method in Chinese Simmental cattle and their associations with IMF traits were analyzed using the general linear model (GLM). The frequency distribution of SNPs in SCD1 and DGAT1 genes were detected by PCR-SSCP method and analyzed in seven other cattle populations. The results showed significant associations of SNPs SCD1 -878, SCD1 -762, and DGAT1 10433 and 10434 with IMF (%) and shearing force values (SFV; kg) in Chinese Simmental cattle. A haplotype combining SCD1 -878C, SCD1 -762T, and DGAT1 10433 and 10434-GC had the highest IMF, marbling score and shearing force. The polymorphic investigation indicated that the frequency of SCD1 -878C or SCD1 -762T was significantly higher in Chinese southern cattle (Leiqiong, Yunnan High pump, BMY or Minnan Cattle) than in Chinese northern cattle (Chinese Simmental, Luxi Cattle, Bohai Black or Chinese Holstein), while the frequency of DGAT1 10433 and 10434-GC in Chinese indigenous breed (Leiqiong, Yunnan High pump, BMY, Luxi Cattle, Bohai Black, or Minnan Cattle) was significantly lower than breeds with imported blood (Chinese Simmental or Chinese Holstein). These findings demonstrated that both the SCD1 and DGAT1 SNPs were prospect genetic markers for IMF traits, and the SCD1 SNPs could be used as a genetic marker for southern or northern blood in Chinese cattle.
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X. ; Yang, Z. P. ; Shi, X. K. ; Li, J. Y. ; Ji, D. J. ; Mao, Y. J. ; Chang, L. L. ; Gao, H. J.</creator><creatorcontrib>Wu, X. X. ; Yang, Z. P. ; Shi, X. K. ; Li, J. Y. ; Ji, D. J. ; Mao, Y. J. ; Chang, L. L. ; Gao, H. J.</creatorcontrib><description>Intramuscular fat (IMF) is a key parameter for evaluation of nutritional quality of beef, with its endogenous synthesis regulated by stearoyl CoA desaturase ( SCD1 ) and diacylglycerol-acyl transferase 1 ( DGAT1 ) genes in cattle. The object of this research was to evaluate the effect of SCD1 and DGAT1 polymorphisms on IMF trait in beef cattle and to estimate the frequency distribution of SNPs in the two genes in Chinese cattle populations. The SCD1 and DGAT1 polymorphisms were detected by PCR-single strand conformation polymorphism (PCR-SSCP) method in Chinese Simmental cattle and their associations with IMF traits were analyzed using the general linear model (GLM). The frequency distribution of SNPs in SCD1 and DGAT1 genes were detected by PCR-SSCP method and analyzed in seven other cattle populations. The results showed significant associations of SNPs SCD1 -878, SCD1 -762, and DGAT1 10433 and 10434 with IMF (%) and shearing force values (SFV; kg) in Chinese Simmental cattle. A haplotype combining SCD1 -878C, SCD1 -762T, and DGAT1 10433 and 10434-GC had the highest IMF, marbling score and shearing force. The polymorphic investigation indicated that the frequency of SCD1 -878C or SCD1 -762T was significantly higher in Chinese southern cattle (Leiqiong, Yunnan High pump, BMY or Minnan Cattle) than in Chinese northern cattle (Chinese Simmental, Luxi Cattle, Bohai Black or Chinese Holstein), while the frequency of DGAT1 10433 and 10434-GC in Chinese indigenous breed (Leiqiong, Yunnan High pump, BMY, Luxi Cattle, Bohai Black, or Minnan Cattle) was significantly lower than breeds with imported blood (Chinese Simmental or Chinese Holstein). 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X.</creatorcontrib><creatorcontrib>Yang, Z. P.</creatorcontrib><creatorcontrib>Shi, X. K.</creatorcontrib><creatorcontrib>Li, J. Y.</creatorcontrib><creatorcontrib>Ji, D. J.</creatorcontrib><creatorcontrib>Mao, Y. J.</creatorcontrib><creatorcontrib>Chang, L. L.</creatorcontrib><creatorcontrib>Gao, H. J.</creatorcontrib><title>Association of SCD1 and DGAT1 SNPs with the intramuscular fat traits in Chinese Simmental cattle and their distribution in eight Chinese cattle breeds</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Intramuscular fat (IMF) is a key parameter for evaluation of nutritional quality of beef, with its endogenous synthesis regulated by stearoyl CoA desaturase ( SCD1 ) and diacylglycerol-acyl transferase 1 ( DGAT1 ) genes in cattle. The object of this research was to evaluate the effect of SCD1 and DGAT1 polymorphisms on IMF trait in beef cattle and to estimate the frequency distribution of SNPs in the two genes in Chinese cattle populations. The SCD1 and DGAT1 polymorphisms were detected by PCR-single strand conformation polymorphism (PCR-SSCP) method in Chinese Simmental cattle and their associations with IMF traits were analyzed using the general linear model (GLM). The frequency distribution of SNPs in SCD1 and DGAT1 genes were detected by PCR-SSCP method and analyzed in seven other cattle populations. The results showed significant associations of SNPs SCD1 -878, SCD1 -762, and DGAT1 10433 and 10434 with IMF (%) and shearing force values (SFV; kg) in Chinese Simmental cattle. A haplotype combining SCD1 -878C, SCD1 -762T, and DGAT1 10433 and 10434-GC had the highest IMF, marbling score and shearing force. The polymorphic investigation indicated that the frequency of SCD1 -878C or SCD1 -762T was significantly higher in Chinese southern cattle (Leiqiong, Yunnan High pump, BMY or Minnan Cattle) than in Chinese northern cattle (Chinese Simmental, Luxi Cattle, Bohai Black or Chinese Holstein), while the frequency of DGAT1 10433 and 10434-GC in Chinese indigenous breed (Leiqiong, Yunnan High pump, BMY, Luxi Cattle, Bohai Black, or Minnan Cattle) was significantly lower than breeds with imported blood (Chinese Simmental or Chinese Holstein). 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X.</au><au>Yang, Z. P.</au><au>Shi, X. K.</au><au>Li, J. Y.</au><au>Ji, D. J.</au><au>Mao, Y. J.</au><au>Chang, L. L.</au><au>Gao, H. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of SCD1 and DGAT1 SNPs with the intramuscular fat traits in Chinese Simmental cattle and their distribution in eight Chinese cattle breeds</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>39</volume><issue>2</issue><spage>1065</spage><epage>1071</epage><pages>1065-1071</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Intramuscular fat (IMF) is a key parameter for evaluation of nutritional quality of beef, with its endogenous synthesis regulated by stearoyl CoA desaturase ( SCD1 ) and diacylglycerol-acyl transferase 1 ( DGAT1 ) genes in cattle. The object of this research was to evaluate the effect of SCD1 and DGAT1 polymorphisms on IMF trait in beef cattle and to estimate the frequency distribution of SNPs in the two genes in Chinese cattle populations. The SCD1 and DGAT1 polymorphisms were detected by PCR-single strand conformation polymorphism (PCR-SSCP) method in Chinese Simmental cattle and their associations with IMF traits were analyzed using the general linear model (GLM). The frequency distribution of SNPs in SCD1 and DGAT1 genes were detected by PCR-SSCP method and analyzed in seven other cattle populations. The results showed significant associations of SNPs SCD1 -878, SCD1 -762, and DGAT1 10433 and 10434 with IMF (%) and shearing force values (SFV; kg) in Chinese Simmental cattle. A haplotype combining SCD1 -878C, SCD1 -762T, and DGAT1 10433 and 10434-GC had the highest IMF, marbling score and shearing force. The polymorphic investigation indicated that the frequency of SCD1 -878C or SCD1 -762T was significantly higher in Chinese southern cattle (Leiqiong, Yunnan High pump, BMY or Minnan Cattle) than in Chinese northern cattle (Chinese Simmental, Luxi Cattle, Bohai Black or Chinese Holstein), while the frequency of DGAT1 10433 and 10434-GC in Chinese indigenous breed (Leiqiong, Yunnan High pump, BMY, Luxi Cattle, Bohai Black, or Minnan Cattle) was significantly lower than breeds with imported blood (Chinese Simmental or Chinese Holstein). These findings demonstrated that both the SCD1 and DGAT1 SNPs were prospect genetic markers for IMF traits, and the SCD1 SNPs could be used as a genetic marker for southern or northern blood in Chinese cattle.</abstract><cop>Dordrecht</cop><pub>Kluwer Academic Publishers</pub><pmid>21607624</pmid><doi>10.1007/s11033-011-0832-0</doi><tpages>7</tpages></addata></record>
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subjects Adipose Tissue, White - physiology
Animal Anatomy
Animal Biochemistry
Animals
Beef
Biomedical and Life Sciences
Body Composition - genetics
Breeding - methods
Cattle
Cattle - genetics
China
Diacylglycerol O-Acyltransferase - genetics
DNA Primers - genetics
Gene Frequency
Genes
Genetic Association Studies
Genetic Markers - genetics
Genotype
Histology
Life Sciences
Linear Models
Meat
Meat quality
Molecular biology
Morphology
Muscle, Skeletal - physiology
Polymerase Chain Reaction
Polymorphism
Polymorphism, Single Nucleotide - genetics
Polymorphism, Single-Stranded Conformational
Species Specificity
Stearoyl-CoA Desaturase - genetics
title Association of SCD1 and DGAT1 SNPs with the intramuscular fat traits in Chinese Simmental cattle and their distribution in eight Chinese cattle breeds
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