Comparative study of the interaction of synthetic methionine-enkephalin and its amidated derivate with monolayers of zwitterionic and negatively charged phospholipids
Using Langmuir’s monolayer technique, the surface behavior and the interaction of the synthetic neuropeptide methionine-enkephalin (Met-enk) and its amidated derivate (Met-enk-NH 2 ) with monolayers of the zwitterionic dimyristoylphosphatidylcholine (DMPC) and the negatively charged dimyristoylphosp...
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| Veröffentlicht in: | Amino acids 2012, Vol.42 (1), p.253-260 |
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| creator | Tsanova, Asya Jordanova, A. Georgiev, G. As Pajpanova, T. Golovinsky, E. Lalchev, Z. |
| description | Using Langmuir’s monolayer technique, the surface behavior and the interaction of the synthetic neuropeptide methionine-enkephalin (Met-enk) and its amidated derivate (Met-enk-NH
2
) with monolayers of the zwitterionic dimyristoylphosphatidylcholine (DMPC) and the negatively charged dimyristoylphosphatidylglycerol (DMPG) were studied. The surface tension (
γ
, mN/m) of DMPG and DMPC monolayers as a function of time (after injection of the peptide under the interface) was detected. The decrease in
γ
values showed that there was a strong penetration effect of both types of Met-enk molecules into the monolayers, being significantly stronger for the amidated derivate, Met-enk-NH
2
. We suggest that the interaction between the neuropeptides and DMPC was predominantly determined by peptides amphiphilicity, while the electrostatic forces play significant role for the insertion of the cationic Met-enk-NH
2
in DMPG monolayers, especially at high packing densities. Our results demonstrate the potential of lipid monolayers formed in Langmuir’s trough to be successfully used as an elegant and simple membrane models to study lipid–peptide interactions at the air/water interface. |
| doi_str_mv | 10.1007/s00726-010-0803-0 |
| format | Article |
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2
) with monolayers of the zwitterionic dimyristoylphosphatidylcholine (DMPC) and the negatively charged dimyristoylphosphatidylglycerol (DMPG) were studied. The surface tension (
γ
, mN/m) of DMPG and DMPC monolayers as a function of time (after injection of the peptide under the interface) was detected. The decrease in
γ
values showed that there was a strong penetration effect of both types of Met-enk molecules into the monolayers, being significantly stronger for the amidated derivate, Met-enk-NH
2
. We suggest that the interaction between the neuropeptides and DMPC was predominantly determined by peptides amphiphilicity, while the electrostatic forces play significant role for the insertion of the cationic Met-enk-NH
2
in DMPG monolayers, especially at high packing densities. Our results demonstrate the potential of lipid monolayers formed in Langmuir’s trough to be successfully used as an elegant and simple membrane models to study lipid–peptide interactions at the air/water interface.</description><identifier>ISSN: 0939-4451</identifier><identifier>EISSN: 1438-2199</identifier><identifier>DOI: 10.1007/s00726-010-0803-0</identifier><identifier>PMID: 21080013</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Amides - chemical synthesis ; Amides - chemistry ; Amino acids ; Analytical Chemistry ; Biochemical Engineering ; Biochemistry ; Biomedical and Life Sciences ; Cationic ; Charging ; Dimyristoylphosphatidylcholine - chemistry ; Enkephalins - chemical synthesis ; Enkephalins - chemistry ; Insertion ; Life Sciences ; Lipids ; Membranes, Artificial ; Methionine - chemical synthesis ; Methionine - chemistry ; Monolayers ; Neurobiology ; Original Article ; Packing density ; Peptides ; Phosphatidylglycerols - chemistry ; Proteomics ; Surface Tension</subject><ispartof>Amino acids, 2012, Vol.42 (1), p.253-260</ispartof><rights>Springer-Verlag 2010</rights><rights>Springer-Verlag 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-276a2c2c8b6800c9ccb2bf3ca43a8028e2b17fa9e1067dd39b34b09f06dc9ade3</citedby><cites>FETCH-LOGICAL-c404t-276a2c2c8b6800c9ccb2bf3ca43a8028e2b17fa9e1067dd39b34b09f06dc9ade3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00726-010-0803-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00726-010-0803-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21080013$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsanova, Asya</creatorcontrib><creatorcontrib>Jordanova, A.</creatorcontrib><creatorcontrib>Georgiev, G. As</creatorcontrib><creatorcontrib>Pajpanova, T.</creatorcontrib><creatorcontrib>Golovinsky, E.</creatorcontrib><creatorcontrib>Lalchev, Z.</creatorcontrib><title>Comparative study of the interaction of synthetic methionine-enkephalin and its amidated derivate with monolayers of zwitterionic and negatively charged phospholipids</title><title>Amino acids</title><addtitle>Amino Acids</addtitle><addtitle>Amino Acids</addtitle><description>Using Langmuir’s monolayer technique, the surface behavior and the interaction of the synthetic neuropeptide methionine-enkephalin (Met-enk) and its amidated derivate (Met-enk-NH
2
) with monolayers of the zwitterionic dimyristoylphosphatidylcholine (DMPC) and the negatively charged dimyristoylphosphatidylglycerol (DMPG) were studied. The surface tension (
γ
, mN/m) of DMPG and DMPC monolayers as a function of time (after injection of the peptide under the interface) was detected. The decrease in
γ
values showed that there was a strong penetration effect of both types of Met-enk molecules into the monolayers, being significantly stronger for the amidated derivate, Met-enk-NH
2
. We suggest that the interaction between the neuropeptides and DMPC was predominantly determined by peptides amphiphilicity, while the electrostatic forces play significant role for the insertion of the cationic Met-enk-NH
2
in DMPG monolayers, especially at high packing densities. Our results demonstrate the potential of lipid monolayers formed in Langmuir’s trough to be successfully used as an elegant and simple membrane models to study lipid–peptide interactions at the air/water interface.</description><subject>Amides - chemical synthesis</subject><subject>Amides - chemistry</subject><subject>Amino acids</subject><subject>Analytical Chemistry</subject><subject>Biochemical Engineering</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cationic</subject><subject>Charging</subject><subject>Dimyristoylphosphatidylcholine - chemistry</subject><subject>Enkephalins - chemical synthesis</subject><subject>Enkephalins - chemistry</subject><subject>Insertion</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Membranes, Artificial</subject><subject>Methionine - chemical synthesis</subject><subject>Methionine - chemistry</subject><subject>Monolayers</subject><subject>Neurobiology</subject><subject>Original Article</subject><subject>Packing density</subject><subject>Peptides</subject><subject>Phosphatidylglycerols - chemistry</subject><subject>Proteomics</subject><subject>Surface Tension</subject><issn>0939-4451</issn><issn>1438-2199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc2OFSEQhYnROHdGH8CNIXHhbFoL6D-W5sa_ZBI3uu7QUH2bsRta4M6kfSCfU3ruaIyJLoDK4asDlUPIMwavGEDzOuaN1wUwKKAFUcADsmOlaAvOpHxIdiCFLMqyYmfkPMZrAMZbVj8mZ5xlHpjYkR97Py8qqGRvkMZ0NCv1A00jUusSBqWT9W6T4uqymqymM6Yxi9Zhge4rLqOarKPKGWpTpGq2RiU01GCwN7mitzaNdPbOT2rFEDez71nL7puLvut0eLj7wrRSPapwyP3L6GNek12siU_Io0FNEZ_enxfky7u3n_cfiqtP7z_u31wVuoQyFbypFddct32d59NS6573g9CqFKoF3iLvWTMoiQzqxhghe1H2IAeojZbKoLggL0--S_DfjhhTN9uocZqUQ3-MnWSiLKGSTSYv_0uyumGiYrWEjL74C732x-DyHB0DWVWiZbzOFDtROvgYAw7dEuyswpqhbou7O8Xd5bi7Le5uc35-73zsZzS_O37lmwF-AmK-cgcMfz79L9ef7oC5mA</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Tsanova, Asya</creator><creator>Jordanova, A.</creator><creator>Georgiev, G. 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As ; Pajpanova, T. ; Golovinsky, E. ; Lalchev, Z.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-276a2c2c8b6800c9ccb2bf3ca43a8028e2b17fa9e1067dd39b34b09f06dc9ade3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amides - chemical synthesis</topic><topic>Amides - chemistry</topic><topic>Amino acids</topic><topic>Analytical Chemistry</topic><topic>Biochemical Engineering</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cationic</topic><topic>Charging</topic><topic>Dimyristoylphosphatidylcholine - chemistry</topic><topic>Enkephalins - chemical synthesis</topic><topic>Enkephalins - chemistry</topic><topic>Insertion</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Membranes, Artificial</topic><topic>Methionine - chemical synthesis</topic><topic>Methionine - chemistry</topic><topic>Monolayers</topic><topic>Neurobiology</topic><topic>Original Article</topic><topic>Packing density</topic><topic>Peptides</topic><topic>Phosphatidylglycerols - chemistry</topic><topic>Proteomics</topic><topic>Surface Tension</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsanova, Asya</creatorcontrib><creatorcontrib>Jordanova, A.</creatorcontrib><creatorcontrib>Georgiev, G. 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As</au><au>Pajpanova, T.</au><au>Golovinsky, E.</au><au>Lalchev, Z.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative study of the interaction of synthetic methionine-enkephalin and its amidated derivate with monolayers of zwitterionic and negatively charged phospholipids</atitle><jtitle>Amino acids</jtitle><stitle>Amino Acids</stitle><addtitle>Amino Acids</addtitle><date>2012</date><risdate>2012</risdate><volume>42</volume><issue>1</issue><spage>253</spage><epage>260</epage><pages>253-260</pages><issn>0939-4451</issn><eissn>1438-2199</eissn><abstract>Using Langmuir’s monolayer technique, the surface behavior and the interaction of the synthetic neuropeptide methionine-enkephalin (Met-enk) and its amidated derivate (Met-enk-NH
2
) with monolayers of the zwitterionic dimyristoylphosphatidylcholine (DMPC) and the negatively charged dimyristoylphosphatidylglycerol (DMPG) were studied. The surface tension (
γ
, mN/m) of DMPG and DMPC monolayers as a function of time (after injection of the peptide under the interface) was detected. The decrease in
γ
values showed that there was a strong penetration effect of both types of Met-enk molecules into the monolayers, being significantly stronger for the amidated derivate, Met-enk-NH
2
. We suggest that the interaction between the neuropeptides and DMPC was predominantly determined by peptides amphiphilicity, while the electrostatic forces play significant role for the insertion of the cationic Met-enk-NH
2
in DMPG monolayers, especially at high packing densities. Our results demonstrate the potential of lipid monolayers formed in Langmuir’s trough to be successfully used as an elegant and simple membrane models to study lipid–peptide interactions at the air/water interface.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>21080013</pmid><doi>10.1007/s00726-010-0803-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Amino acids, 2012, Vol.42 (1), p.253-260 |
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| language | eng |
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| subjects | Amides - chemical synthesis Amides - chemistry Amino acids Analytical Chemistry Biochemical Engineering Biochemistry Biomedical and Life Sciences Cationic Charging Dimyristoylphosphatidylcholine - chemistry Enkephalins - chemical synthesis Enkephalins - chemistry Insertion Life Sciences Lipids Membranes, Artificial Methionine - chemical synthesis Methionine - chemistry Monolayers Neurobiology Original Article Packing density Peptides Phosphatidylglycerols - chemistry Proteomics Surface Tension |
| title | Comparative study of the interaction of synthetic methionine-enkephalin and its amidated derivate with monolayers of zwitterionic and negatively charged phospholipids |
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