Gold nanoparticle-based fluorescence immunoassay for malaria antigen detection

The development of rapid detection assays for malaria diagnostics is an area of intensive research, as the traditional microscopic analysis of blood smears is cumbersome and requires skilled personnel. Here, we describe a simple and sensitive immunoassay that successfully detects malaria antigens in...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2012-01, Vol.402 (3), p.1019-1027
Hauptverfasser:	Guirgis, Bassem S. S., Sá e Cunha, Cláudia, Gomes, Inês, Cavadas, Miguel, Silva, Isabel, Doria, Gonçalo, Blatch, Gregory L., Baptista, Pedro V., Pereira, Eulália, Azzazy, Hassan M. E., Mota, Maria M., Prudêncio, Miguel, Franco, Ricardo
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Analysis Analytical Chemistry Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Antigens Antigens, Protozoan - analysis Antigens, Protozoan - immunology Biochemistry Blood Carbocyanines - chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Culture Fluorescence Fluorescent Antibody Technique - methods Fluorescent Dyes - chemistry Food Science Gold - chemistry Heat shock proteins Hostages HSP70 Heat-Shock Proteins - analysis HSP70 Heat-Shock Proteins - immunology Humans Immunoassay Laboratory Medicine Malaria Malaria - blood Malaria - diagnosis Malaria - immunology Monitoring/Environmental Analysis Monoclonal antibodies Nanoparticles - chemistry Nanostructure Original Paper Plasmodium falciparum Plasmodium falciparum - immunology Plasmodium falciparum - isolation & purification Protein binding Protozoan Proteins - analysis Protozoan Proteins - immunology Recombinant Proteins - analysis Recombinant Proteins - immunology Sensitivity and Specificity Surface chemistry
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