Altered expression pattern of testican-1 mRNA after brain injury
Testican, a chondroitin/heparan sulfate proteoglycan, is primarily expressed in neurons of the adult and embryonic mouse brain, suggesting its role in normal and/or proliferation and differentiation processes of neurons. However, the role of testican in injured brain remains unclear. In the present...
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| Veröffentlicht in: | Biomedical Research 2011, Vol.32(6), pp.373-378 |
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| creator | Iseki, Ken Hagino, Seita Zhang, Yuxiang Mori, Tetsuji Sato, Nobuko Yokoya, Sachihiko Hozumi, Yasukazu Goto, Kaoru Tase, Choichiro |
| description | Testican, a chondroitin/heparan sulfate proteoglycan, is primarily expressed in neurons of the adult and embryonic mouse brain, suggesting its role in normal and/or proliferation and differentiation processes of neurons. However, the role of testican in injured brain remains unclear. In the present study we investigated testican-1 mRNA expression pattern after cryo-injury of the brain. In situ hybridization histochemistry revealed that testican-1 mRNA is induced in the region surrounding the necrotic tissue. Time course study of testican-1 mRNA showed the highest level of signal intensity at 7 days after the injury. To determine which cell types express testican-1 mRNA, we performed in situ hybridization histochemistry combined with immunohistochemistry of several cell markers. Testican-1 mRNA signals were detected in the proximal reactive astrocytes, whereas the distribution pattern of testican-1 mRNA positive cells was different from those of mature oligodendrocytes and activated microglia. In addition, signals for testican-1 mRNA overlapped with those of FGF-2 mRNA, showing that these molecules are coexpressed in reactive astrocytes. These results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury. |
| doi_str_mv | 10.2220/biomedres.32.373 |
| format | Article |
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However, the role of testican in injured brain remains unclear. In the present study we investigated testican-1 mRNA expression pattern after cryo-injury of the brain. In situ hybridization histochemistry revealed that testican-1 mRNA is induced in the region surrounding the necrotic tissue. Time course study of testican-1 mRNA showed the highest level of signal intensity at 7 days after the injury. To determine which cell types express testican-1 mRNA, we performed in situ hybridization histochemistry combined with immunohistochemistry of several cell markers. Testican-1 mRNA signals were detected in the proximal reactive astrocytes, whereas the distribution pattern of testican-1 mRNA positive cells was different from those of mature oligodendrocytes and activated microglia. In addition, signals for testican-1 mRNA overlapped with those of FGF-2 mRNA, showing that these molecules are coexpressed in reactive astrocytes. These results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury.</description><identifier>ISSN: 0388-6107</identifier><identifier>EISSN: 1880-313X</identifier><identifier>DOI: 10.2220/biomedres.32.373</identifier><identifier>PMID: 22199127</identifier><language>eng</language><publisher>Japan: Biomedical Research Press</publisher><subject>Animals ; Astrocytes ; Axons ; Brain Injuries - genetics ; Brain injury ; Differentiation ; Embryos ; Fibroblast growth factor 2 ; Gene expression ; Heparan sulfate proteoglycans ; Histochemistry ; Immunohistochemistry ; In Situ Hybridization ; Male ; Mice ; Microglia ; Microscopy, Confocal ; Neurons ; Oligodendrocytes ; Proteoglycans - genetics ; RNA, Messenger - genetics</subject><ispartof>Biomedical Research, 2011, Vol.32(6), pp.373-378</ispartof><rights>2011 Biomedical Research Press</rights><rights>Copyright Japan Science and Technology Agency 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c654t-9cfd99fdfb4d04711506610c2a0b418d13ccfb0fcba5d716208d68917c5153993</citedby><cites>FETCH-LOGICAL-c654t-9cfd99fdfb4d04711506610c2a0b418d13ccfb0fcba5d716208d68917c5153993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22199127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iseki, Ken</creatorcontrib><creatorcontrib>Hagino, Seita</creatorcontrib><creatorcontrib>Zhang, Yuxiang</creatorcontrib><creatorcontrib>Mori, Tetsuji</creatorcontrib><creatorcontrib>Sato, Nobuko</creatorcontrib><creatorcontrib>Yokoya, Sachihiko</creatorcontrib><creatorcontrib>Hozumi, Yasukazu</creatorcontrib><creatorcontrib>Goto, Kaoru</creatorcontrib><creatorcontrib>Tase, Choichiro</creatorcontrib><title>Altered expression pattern of testican-1 mRNA after brain injury</title><title>Biomedical Research</title><addtitle>Biomed. Res.</addtitle><description>Testican, a chondroitin/heparan sulfate proteoglycan, is primarily expressed in neurons of the adult and embryonic mouse brain, suggesting its role in normal and/or proliferation and differentiation processes of neurons. However, the role of testican in injured brain remains unclear. In the present study we investigated testican-1 mRNA expression pattern after cryo-injury of the brain. In situ hybridization histochemistry revealed that testican-1 mRNA is induced in the region surrounding the necrotic tissue. Time course study of testican-1 mRNA showed the highest level of signal intensity at 7 days after the injury. To determine which cell types express testican-1 mRNA, we performed in situ hybridization histochemistry combined with immunohistochemistry of several cell markers. Testican-1 mRNA signals were detected in the proximal reactive astrocytes, whereas the distribution pattern of testican-1 mRNA positive cells was different from those of mature oligodendrocytes and activated microglia. In addition, signals for testican-1 mRNA overlapped with those of FGF-2 mRNA, showing that these molecules are coexpressed in reactive astrocytes. These results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury.</description><subject>Animals</subject><subject>Astrocytes</subject><subject>Axons</subject><subject>Brain Injuries - genetics</subject><subject>Brain injury</subject><subject>Differentiation</subject><subject>Embryos</subject><subject>Fibroblast growth factor 2</subject><subject>Gene expression</subject><subject>Heparan sulfate proteoglycans</subject><subject>Histochemistry</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Mice</subject><subject>Microglia</subject><subject>Microscopy, Confocal</subject><subject>Neurons</subject><subject>Oligodendrocytes</subject><subject>Proteoglycans - genetics</subject><subject>RNA, Messenger - genetics</subject><issn>0388-6107</issn><issn>1880-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1LAzEQxYMoWqt3T7LgQS9bM8luPm6WUj-gKIiCt5DNJrplu1uTXbD_vSmtBT14SUjebx4z8xA6AzwihODromoXtvQ2jCgZUU730ACEwCkF-raPBpgKkTLA_AgdhzDH8Q2CHqIjQkBKIHyAbsZ1Z70tE_u1jD6haptkqbv41yStSzobusroJoVk8fw4TrSLSlJ4XTVJ1cx7vzpBB07XwZ5u7yF6vZ2-TO7T2dPdw2Q8Sw3Lsy6VxpVSutIVWYkzDpBjFjszROMiA1ECNcYV2JlC5yUHRrAomZDATQ45lZIO0eXGd-nbzz62pRZVMLaudWPbPqj1ODIngkXy6l8SsKRZnkuBI3rxB523vW_iHAoyxjnLSEYjhTeU8W0I3jq19NVC-1W0Uusc1C4HRYmKOcSS861xX0RlV_Cz-AhMN8A8dPrd7gDt475r-9uRbQ9Od7r50F7Zhn4DBxSdSw</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Iseki, Ken</creator><creator>Hagino, Seita</creator><creator>Zhang, Yuxiang</creator><creator>Mori, Tetsuji</creator><creator>Sato, Nobuko</creator><creator>Yokoya, Sachihiko</creator><creator>Hozumi, Yasukazu</creator><creator>Goto, Kaoru</creator><creator>Tase, Choichiro</creator><general>Biomedical Research Press</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20110101</creationdate><title>Altered expression pattern of testican-1 mRNA after brain injury</title><author>Iseki, Ken ; Hagino, Seita ; Zhang, Yuxiang ; Mori, Tetsuji ; Sato, Nobuko ; Yokoya, Sachihiko ; Hozumi, Yasukazu ; Goto, Kaoru ; Tase, Choichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c654t-9cfd99fdfb4d04711506610c2a0b418d13ccfb0fcba5d716208d68917c5153993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Astrocytes</topic><topic>Axons</topic><topic>Brain Injuries - genetics</topic><topic>Brain injury</topic><topic>Differentiation</topic><topic>Embryos</topic><topic>Fibroblast growth factor 2</topic><topic>Gene expression</topic><topic>Heparan sulfate proteoglycans</topic><topic>Histochemistry</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Mice</topic><topic>Microglia</topic><topic>Microscopy, Confocal</topic><topic>Neurons</topic><topic>Oligodendrocytes</topic><topic>Proteoglycans - genetics</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iseki, Ken</creatorcontrib><creatorcontrib>Hagino, Seita</creatorcontrib><creatorcontrib>Zhang, Yuxiang</creatorcontrib><creatorcontrib>Mori, Tetsuji</creatorcontrib><creatorcontrib>Sato, Nobuko</creatorcontrib><creatorcontrib>Yokoya, Sachihiko</creatorcontrib><creatorcontrib>Hozumi, Yasukazu</creatorcontrib><creatorcontrib>Goto, Kaoru</creatorcontrib><creatorcontrib>Tase, Choichiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iseki, Ken</au><au>Hagino, Seita</au><au>Zhang, Yuxiang</au><au>Mori, Tetsuji</au><au>Sato, Nobuko</au><au>Yokoya, Sachihiko</au><au>Hozumi, Yasukazu</au><au>Goto, Kaoru</au><au>Tase, Choichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered expression pattern of testican-1 mRNA after brain injury</atitle><jtitle>Biomedical Research</jtitle><addtitle>Biomed. Res.</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>32</volume><issue>6</issue><spage>373</spage><epage>378</epage><pages>373-378</pages><issn>0388-6107</issn><eissn>1880-313X</eissn><abstract>Testican, a chondroitin/heparan sulfate proteoglycan, is primarily expressed in neurons of the adult and embryonic mouse brain, suggesting its role in normal and/or proliferation and differentiation processes of neurons. However, the role of testican in injured brain remains unclear. In the present study we investigated testican-1 mRNA expression pattern after cryo-injury of the brain. In situ hybridization histochemistry revealed that testican-1 mRNA is induced in the region surrounding the necrotic tissue. Time course study of testican-1 mRNA showed the highest level of signal intensity at 7 days after the injury. To determine which cell types express testican-1 mRNA, we performed in situ hybridization histochemistry combined with immunohistochemistry of several cell markers. 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| subjects | Animals Astrocytes Axons Brain Injuries - genetics Brain injury Differentiation Embryos Fibroblast growth factor 2 Gene expression Heparan sulfate proteoglycans Histochemistry Immunohistochemistry In Situ Hybridization Male Mice Microglia Microscopy, Confocal Neurons Oligodendrocytes Proteoglycans - genetics RNA, Messenger - genetics |
| title | Altered expression pattern of testican-1 mRNA after brain injury |
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