Detection of Estrogen- and Dioxin-like Activity in Pulp and Paper Mill Black Liquor and Effluent Using in vitro Recombinant Receptor/Reporter Gene Assays
Methanol extracts of pulp and paper mill effluent (MF) and black liquor, a recovered by-product, were examined by bioassays for dioxin-like, estrogenic and anti-estrogenic activities. Black liquor was estimated as containing 4 ppb estrogen equivalents using MCF-7 cells transiently transfected with a...
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| Veröffentlicht in: | Environmental science & technology 1995-08, Vol.29 (8), p.2140-2146 |
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| creator | Zacharewski, Timothy R Berhane, Kiflu Gillesby, Bradley E Burnison, B. Kent |
| description | Methanol extracts of pulp and paper mill effluent (MF) and black liquor, a recovered by-product, were examined by bioassays for dioxin-like, estrogenic and anti-estrogenic activities. Black liquor was estimated as containing 4 ppb estrogen equivalents using MCF-7 cells transiently transfected with a Gal4-estrogen receptor guchimeri construct and a Gal4-regulated luciferase reporter gene. MF had negligible estrogenic activity. A dioxin response element-regulated luciferase reporter gene (pGudLuc 1.1) transiently transfected into Hepa1c1c7 wild-type cells showed a dose-dependent increase in luciferase activity following treatment with MF, black liquor and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Black liquor and MF contained 10 and 20 ppb TCDD equivalents. MF also had significant Ah receptor-mediated anti-estrogenic activity. The effects of MF on fish could be due to Ah receptor and ER ligands not detected by chemical analysis. There are 78 references. |
| doi_str_mv | 10.1021/es00008a037 |
| format | Article |
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Kent</creator><creatorcontrib>Zacharewski, Timothy R ; Berhane, Kiflu ; Gillesby, Bradley E ; Burnison, B. Kent</creatorcontrib><description>Methanol extracts of pulp and paper mill effluent (MF) and black liquor, a recovered by-product, were examined by bioassays for dioxin-like, estrogenic and anti-estrogenic activities. Black liquor was estimated as containing 4 ppb estrogen equivalents using MCF-7 cells transiently transfected with a Gal4-estrogen receptor guchimeri construct and a Gal4-regulated luciferase reporter gene. MF had negligible estrogenic activity. A dioxin response element-regulated luciferase reporter gene (pGudLuc 1.1) transiently transfected into Hepa1c1c7 wild-type cells showed a dose-dependent increase in luciferase activity following treatment with MF, black liquor and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Black liquor and MF contained 10 and 20 ppb TCDD equivalents. MF also had significant Ah receptor-mediated anti-estrogenic activity. The effects of MF on fish could be due to Ah receptor and ER ligands not detected by chemical analysis. 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Kent</creatorcontrib><title>Detection of Estrogen- and Dioxin-like Activity in Pulp and Paper Mill Black Liquor and Effluent Using in vitro Recombinant Receptor/Reporter Gene Assays</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>Methanol extracts of pulp and paper mill effluent (MF) and black liquor, a recovered by-product, were examined by bioassays for dioxin-like, estrogenic and anti-estrogenic activities. Black liquor was estimated as containing 4 ppb estrogen equivalents using MCF-7 cells transiently transfected with a Gal4-estrogen receptor guchimeri construct and a Gal4-regulated luciferase reporter gene. MF had negligible estrogenic activity. A dioxin response element-regulated luciferase reporter gene (pGudLuc 1.1) transiently transfected into Hepa1c1c7 wild-type cells showed a dose-dependent increase in luciferase activity following treatment with MF, black liquor and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Black liquor and MF contained 10 and 20 ppb TCDD equivalents. MF also had significant Ah receptor-mediated anti-estrogenic activity. The effects of MF on fish could be due to Ah receptor and ER ligands not detected by chemical analysis. 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Kent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Estrogen- and Dioxin-like Activity in Pulp and Paper Mill Black Liquor and Effluent Using in vitro Recombinant Receptor/Reporter Gene Assays</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>1995-08-01</date><risdate>1995</risdate><volume>29</volume><issue>8</issue><spage>2140</spage><epage>2146</epage><pages>2140-2146</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><coden>ESTHAG</coden><abstract>Methanol extracts of pulp and paper mill effluent (MF) and black liquor, a recovered by-product, were examined by bioassays for dioxin-like, estrogenic and anti-estrogenic activities. Black liquor was estimated as containing 4 ppb estrogen equivalents using MCF-7 cells transiently transfected with a Gal4-estrogen receptor guchimeri construct and a Gal4-regulated luciferase reporter gene. MF had negligible estrogenic activity. 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| source | ACS Publications |
| subjects | Applied sciences Chemistry Dioxins Effluents Exact sciences and technology Freshwater Industrial wastewaters Pollution Pulp & paper mills Wastewaters Water treatment and pollution |
| title | Detection of Estrogen- and Dioxin-like Activity in Pulp and Paper Mill Black Liquor and Effluent Using in vitro Recombinant Receptor/Reporter Gene Assays |
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