The genetic and structural basis of two distinct terminal side branch residues in stewartan and amylovoran exopolysaccharides and their potential role in host adaptation
Summary Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set of homologou...
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Veröffentlicht in: | Molecular microbiology 2012-01, Vol.83 (1), p.195-207 |
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Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set of homologous operons that have been partially characterized, although some annotations are solely on the basis of sequence homology. The major distinguishing features of these two EPS forms are the presence of a terminal pyruvate in amylovoran and glucose in stewartan, even though the gene systems to account for both are conserved and present in each bacterium. This study explores the genetic, structural and functional differences of amylovoran and stewartan, and their potential role in host adaptation. We report that the pyruvyl transferase gene in P. stewartii is non‐functional, while the terminal glucosyl transferase is catalytically active. Conversely, in E. amylovora, the homologous glucosyl transferase activity appears to be relatively ineffective, while the pyruvyl transferase function predominates. We also show that the terminally pyruvylated versus glucosylated EPS require specific repeating unit translocases (Wzx). We discuss the evolutionary, functional and biological implications of the terminally pyruvylated and glucosylated polymers and their potential contribution to plant and insect host adaptation. |
doi_str_mv | 10.1111/j.1365-2958.2011.07926.x |
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Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set of homologous operons that have been partially characterized, although some annotations are solely on the basis of sequence homology. The major distinguishing features of these two EPS forms are the presence of a terminal pyruvate in amylovoran and glucose in stewartan, even though the gene systems to account for both are conserved and present in each bacterium. This study explores the genetic, structural and functional differences of amylovoran and stewartan, and their potential role in host adaptation. We report that the pyruvyl transferase gene in P. stewartii is non‐functional, while the terminal glucosyl transferase is catalytically active. Conversely, in E. amylovora, the homologous glucosyl transferase activity appears to be relatively ineffective, while the pyruvyl transferase function predominates. We also show that the terminally pyruvylated versus glucosylated EPS require specific repeating unit translocases (Wzx). We discuss the evolutionary, functional and biological implications of the terminally pyruvylated and glucosylated polymers and their potential contribution to plant and insect host adaptation.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2011.07926.x</identifier><identifier>PMID: 22111898</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biological and medical sciences ; Biosynthesis ; Carbohydrate Sequence ; Erwinia - genetics ; Erwinia - metabolism ; Erwinia - pathogenicity ; Erwinia amylovora ; Evolutionary biology ; Flowers & plants ; Fundamental and applied biological sciences. Psychology ; Genetics ; Gram-negative bacteria ; Malus - microbiology ; Microbiology ; Molecular Sequence Data ; Operon ; Pantoea - genetics ; Pantoea - metabolism ; Pantoea - pathogenicity ; Pantoea stewartii ; Plant Diseases - microbiology ; Polysaccharides, Bacterial - biosynthesis ; Polysaccharides, Bacterial - chemistry ; Sequence Alignment ; Virulence ; Zea mays - microbiology</subject><ispartof>Molecular microbiology, 2012-01, Vol.83 (1), p.195-207</ispartof><rights>2011 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2011 Blackwell Publishing Ltd.</rights><rights>Copyright Blackwell Publishing Ltd. Jan 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5466-ae9ba3f8fc7b87589d7f39cc8593e6637bbefc1c3bee26bcb4a495c01ce5847d3</citedby><cites>FETCH-LOGICAL-c5466-ae9ba3f8fc7b87589d7f39cc8593e6637bbefc1c3bee26bcb4a495c01ce5847d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.2011.07926.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.2011.07926.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,4024,27923,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25343993$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22111898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xiaolei</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>von Bodman, Susanne B.</creatorcontrib><title>The genetic and structural basis of two distinct terminal side branch residues in stewartan and amylovoran exopolysaccharides and their potential role in host adaptation</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set of homologous operons that have been partially characterized, although some annotations are solely on the basis of sequence homology. The major distinguishing features of these two EPS forms are the presence of a terminal pyruvate in amylovoran and glucose in stewartan, even though the gene systems to account for both are conserved and present in each bacterium. This study explores the genetic, structural and functional differences of amylovoran and stewartan, and their potential role in host adaptation. We report that the pyruvyl transferase gene in P. stewartii is non‐functional, while the terminal glucosyl transferase is catalytically active. Conversely, in E. amylovora, the homologous glucosyl transferase activity appears to be relatively ineffective, while the pyruvyl transferase function predominates. We also show that the terminally pyruvylated versus glucosylated EPS require specific repeating unit translocases (Wzx). We discuss the evolutionary, functional and biological implications of the terminally pyruvylated and glucosylated polymers and their potential contribution to plant and insect host adaptation.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Carbohydrate Sequence</subject><subject>Erwinia - genetics</subject><subject>Erwinia - metabolism</subject><subject>Erwinia - pathogenicity</subject><subject>Erwinia amylovora</subject><subject>Evolutionary biology</subject><subject>Flowers & plants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Gram-negative bacteria</subject><subject>Malus - microbiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Operon</subject><subject>Pantoea - genetics</subject><subject>Pantoea - metabolism</subject><subject>Pantoea - pathogenicity</subject><subject>Pantoea stewartii</subject><subject>Plant Diseases - microbiology</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Polysaccharides, Bacterial - chemistry</subject><subject>Sequence Alignment</subject><subject>Virulence</subject><subject>Zea mays - microbiology</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkkGP1CAYhhujcWdX_4IhJsa9dKTQUjh4WEddN9nVyxiNF0LpV8vYlhGoM_OT_JfSmXFMPBi5FMLzPgHeJgnK8DyL48VqnlFWpEQUfE5wls1xKQibb-8ls9PG_WSGRYFTysnns-Tc-xXGGcWMPkzOCIkWLvgs-blsAX2FAYLRSA018sGNOoxOdahS3nhkGxQ2FtXGBzPogAK43gxx25saUOXUoFvkIK5G8MgM0QAb5YIa9j7V7zr7w0YMwdaubbfzSutWuZj2eyK0YBxa2wBDMNHrbAeTp7U-IFWrdVDB2OFR8qBRnYfHx-9F8vHtm-XiXXr74fpmcXWb6iJnLFUgKkUb3uiy4mXBRV02VGjNC0GBMVpWFTQ607QCIKzSVa5yUWicaSh4Xtb0Inl-8K6d_R6vFGRvvIauUwPY0UuREZbjkpNIXv6TzDDhsZic5RF9-he6sqOLr7j38VwQgiPED5B21nsHjVw70yu3iyY59S5XcqpXTvXKqXe5711uY_TJ0T9WPdSn4O-iI_DsCCivVddMtRn_hytoToWgkXt54Damg91_H0De3d1Ms5hPD_n4u8D2lFfum2QlLQv56f21pItX7DXGX-SS_gIj_NxS</recordid><startdate>201201</startdate><enddate>201201</enddate><creator>Wang, Xiaolei</creator><creator>Yang, Fan</creator><creator>von Bodman, Susanne B.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201201</creationdate><title>The genetic and structural basis of two distinct terminal side branch residues in stewartan and amylovoran exopolysaccharides and their potential role in host adaptation</title><author>Wang, Xiaolei ; Yang, Fan ; von Bodman, Susanne B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5466-ae9ba3f8fc7b87589d7f39cc8593e6637bbefc1c3bee26bcb4a495c01ce5847d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Carbohydrate Sequence</topic><topic>Erwinia - genetics</topic><topic>Erwinia - metabolism</topic><topic>Erwinia - pathogenicity</topic><topic>Erwinia amylovora</topic><topic>Evolutionary biology</topic><topic>Flowers & plants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Gram-negative bacteria</topic><topic>Malus - microbiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Operon</topic><topic>Pantoea - genetics</topic><topic>Pantoea - metabolism</topic><topic>Pantoea - pathogenicity</topic><topic>Pantoea stewartii</topic><topic>Plant Diseases - microbiology</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Polysaccharides, Bacterial - chemistry</topic><topic>Sequence Alignment</topic><topic>Virulence</topic><topic>Zea mays - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xiaolei</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>von Bodman, Susanne B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiaolei</au><au>Yang, Fan</au><au>von Bodman, Susanne B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The genetic and structural basis of two distinct terminal side branch residues in stewartan and amylovoran exopolysaccharides and their potential role in host adaptation</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2012-01</date><risdate>2012</risdate><volume>83</volume><issue>1</issue><spage>195</spage><epage>207</epage><pages>195-207</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set of homologous operons that have been partially characterized, although some annotations are solely on the basis of sequence homology. The major distinguishing features of these two EPS forms are the presence of a terminal pyruvate in amylovoran and glucose in stewartan, even though the gene systems to account for both are conserved and present in each bacterium. This study explores the genetic, structural and functional differences of amylovoran and stewartan, and their potential role in host adaptation. We report that the pyruvyl transferase gene in P. stewartii is non‐functional, while the terminal glucosyl transferase is catalytically active. Conversely, in E. amylovora, the homologous glucosyl transferase activity appears to be relatively ineffective, while the pyruvyl transferase function predominates. We also show that the terminally pyruvylated versus glucosylated EPS require specific repeating unit translocases (Wzx). We discuss the evolutionary, functional and biological implications of the terminally pyruvylated and glucosylated polymers and their potential contribution to plant and insect host adaptation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22111898</pmid><doi>10.1111/j.1365-2958.2011.07926.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological and medical sciences Biosynthesis Carbohydrate Sequence Erwinia - genetics Erwinia - metabolism Erwinia - pathogenicity Erwinia amylovora Evolutionary biology Flowers & plants Fundamental and applied biological sciences. Psychology Genetics Gram-negative bacteria Malus - microbiology Microbiology Molecular Sequence Data Operon Pantoea - genetics Pantoea - metabolism Pantoea - pathogenicity Pantoea stewartii Plant Diseases - microbiology Polysaccharides, Bacterial - biosynthesis Polysaccharides, Bacterial - chemistry Sequence Alignment Virulence Zea mays - microbiology |
title | The genetic and structural basis of two distinct terminal side branch residues in stewartan and amylovoran exopolysaccharides and their potential role in host adaptation |
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