Circulating and tumor-infiltrating myeloid cell subsets in patients with bladder cancer
Both cancer‐related inflammation and tumor‐induced immune suppression are associated with expansion of myeloid cell subsets including myeloid‐derived suppressor cells. However, little known regarding characteristics of myeloid cells in patients with bladder cancer. In this study, we analyzed myeloid...
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| Veröffentlicht in: | International journal of cancer 2012-03, Vol.130 (5), p.1109-1119 |
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| container_title | International journal of cancer |
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| creator | Eruslanov, Evgeniy Neuberger, Molly Daurkin, Irina Perrin, George Q. Algood, Chester Dahm, Philipp Rosser, Charles Vieweg, Johannes Gilbert, Scott M. Kusmartsev, Sergei |
| description | Both cancer‐related inflammation and tumor‐induced immune suppression are associated with expansion of myeloid cell subsets including myeloid‐derived suppressor cells. However, little known regarding characteristics of myeloid cells in patients with bladder cancer. In this study, we analyzed myeloid cells from peripheral blood (PBMC) and tumor tissue that were collected from patients with superficial noninvasive and invasive urothelial carcinomas. Our results demonstrate that PBMC from bladder cancer patients contain two major CD11b myeloid cell subsets: granulocyte‐type CD15high CD33low cells and monocyte‐type CD15low CD33high cells. The number of circulating granulocytic but not monocytic myeloid cells in cancer patients was markedly increased when compared to healthy individuals. Both myeloid cell subsets from cancer patients were highly activated and produced substantial amounts of proinflammatory chemokines/cytokines including CCL2, CCL3, CCL4, G‐CSF, IL‐8 and IL‐6. Granulocytic myeloid cells were able to inhibit in vitro T cell proliferation through induction of CD4+Foxp3+ T regulatory cells. Analysis of bladder cancer tissues revealed that tumors were infiltrated with monocyte–macrophage CD11b+HLA‐DR+ and granulocytic CD11b+CD15+HLA‐DR‐ myeloid cells. Collectively, this study identifies myeloid cell subsets in patients with bladder cancer. We demonstrate that these highly activated inflammatory myeloid cells represent a source of multiple chemokines/cytokines and may contribute to inflammation and immune dysfunction in bladder cancer. |
| doi_str_mv | 10.1002/ijc.26123 |
| format | Article |
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However, little known regarding characteristics of myeloid cells in patients with bladder cancer. In this study, we analyzed myeloid cells from peripheral blood (PBMC) and tumor tissue that were collected from patients with superficial noninvasive and invasive urothelial carcinomas. Our results demonstrate that PBMC from bladder cancer patients contain two major CD11b myeloid cell subsets: granulocyte‐type CD15high CD33low cells and monocyte‐type CD15low CD33high cells. The number of circulating granulocytic but not monocytic myeloid cells in cancer patients was markedly increased when compared to healthy individuals. Both myeloid cell subsets from cancer patients were highly activated and produced substantial amounts of proinflammatory chemokines/cytokines including CCL2, CCL3, CCL4, G‐CSF, IL‐8 and IL‐6. Granulocytic myeloid cells were able to inhibit in vitro T cell proliferation through induction of CD4+Foxp3+ T regulatory cells. Analysis of bladder cancer tissues revealed that tumors were infiltrated with monocyte–macrophage CD11b+HLA‐DR+ and granulocytic CD11b+CD15+HLA‐DR‐ myeloid cells. Collectively, this study identifies myeloid cell subsets in patients with bladder cancer. We demonstrate that these highly activated inflammatory myeloid cells represent a source of multiple chemokines/cytokines and may contribute to inflammation and immune dysfunction in bladder cancer.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.26123</identifier><identifier>PMID: 21480223</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Antigens, CD - metabolism ; Antigens, Differentiation, Myelomonocytic - metabolism ; Biological and medical sciences ; Bladder cancer ; Bone marrow ; Cancer ; CD11 Antigens - metabolism ; Chemokines ; Cytokines ; Cytokines - metabolism ; Granulocytes - immunology ; Humans ; immune suppresision ; Immune Tolerance ; inflammation ; Lewis X Antigen - metabolism ; Lymphocyte Activation ; Medical research ; Medical sciences ; Monocytes - immunology ; myeloid cells ; Myeloid Cells - immunology ; Nephrology. Urinary tract diseases ; Sialic Acid Binding Ig-like Lectin 3 ; tumor-infiltrating myeloid cells ; Tumors ; Tumors of the urinary system ; Urinary Bladder Neoplasms - immunology ; Urinary tract. Prostate gland</subject><ispartof>International journal of cancer, 2012-03, Vol.130 (5), p.1109-1119</ispartof><rights>Copyright © 2011 UICC</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 UICC.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4883-c88f1583c63af4ed64edd86efa67c26635bbbf630d9f21e460c566451a386dd83</citedby><cites>FETCH-LOGICAL-c4883-c88f1583c63af4ed64edd86efa67c26635bbbf630d9f21e460c566451a386dd83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.26123$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.26123$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25533487$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21480223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eruslanov, Evgeniy</creatorcontrib><creatorcontrib>Neuberger, Molly</creatorcontrib><creatorcontrib>Daurkin, Irina</creatorcontrib><creatorcontrib>Perrin, George Q.</creatorcontrib><creatorcontrib>Algood, Chester</creatorcontrib><creatorcontrib>Dahm, Philipp</creatorcontrib><creatorcontrib>Rosser, Charles</creatorcontrib><creatorcontrib>Vieweg, Johannes</creatorcontrib><creatorcontrib>Gilbert, Scott M.</creatorcontrib><creatorcontrib>Kusmartsev, Sergei</creatorcontrib><title>Circulating and tumor-infiltrating myeloid cell subsets in patients with bladder cancer</title><title>International journal of cancer</title><addtitle>Int. J. Cancer</addtitle><description>Both cancer‐related inflammation and tumor‐induced immune suppression are associated with expansion of myeloid cell subsets including myeloid‐derived suppressor cells. However, little known regarding characteristics of myeloid cells in patients with bladder cancer. In this study, we analyzed myeloid cells from peripheral blood (PBMC) and tumor tissue that were collected from patients with superficial noninvasive and invasive urothelial carcinomas. Our results demonstrate that PBMC from bladder cancer patients contain two major CD11b myeloid cell subsets: granulocyte‐type CD15high CD33low cells and monocyte‐type CD15low CD33high cells. The number of circulating granulocytic but not monocytic myeloid cells in cancer patients was markedly increased when compared to healthy individuals. Both myeloid cell subsets from cancer patients were highly activated and produced substantial amounts of proinflammatory chemokines/cytokines including CCL2, CCL3, CCL4, G‐CSF, IL‐8 and IL‐6. Granulocytic myeloid cells were able to inhibit in vitro T cell proliferation through induction of CD4+Foxp3+ T regulatory cells. Analysis of bladder cancer tissues revealed that tumors were infiltrated with monocyte–macrophage CD11b+HLA‐DR+ and granulocytic CD11b+CD15+HLA‐DR‐ myeloid cells. Collectively, this study identifies myeloid cell subsets in patients with bladder cancer. We demonstrate that these highly activated inflammatory myeloid cells represent a source of multiple chemokines/cytokines and may contribute to inflammation and immune dysfunction in bladder cancer.</description><subject>Antigens, CD - metabolism</subject><subject>Antigens, Differentiation, Myelomonocytic - metabolism</subject><subject>Biological and medical sciences</subject><subject>Bladder cancer</subject><subject>Bone marrow</subject><subject>Cancer</subject><subject>CD11 Antigens - metabolism</subject><subject>Chemokines</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Granulocytes - immunology</subject><subject>Humans</subject><subject>immune suppresision</subject><subject>Immune Tolerance</subject><subject>inflammation</subject><subject>Lewis X Antigen - metabolism</subject><subject>Lymphocyte Activation</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Monocytes - immunology</subject><subject>myeloid cells</subject><subject>Myeloid Cells - immunology</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Sialic Acid Binding Ig-like Lectin 3</subject><subject>tumor-infiltrating myeloid cells</subject><subject>Tumors</subject><subject>Tumors of the urinary system</subject><subject>Urinary Bladder Neoplasms - immunology</subject><subject>Urinary tract. Prostate gland</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90c1rFDEUAPAgil2rB_8BGRCxHqbN55vMURbtB4t6qBT2EjKZRLNmZrbJDHX_e9POtgVBDyEh-eW9lzyEXhN8TDCmJ35jjikQyp6gBcF1VWJKxFO0yGe4rAiDA_QipQ3GhAjMn6MDSrjElLIFulr6aKagR9__KHTfFuPUDbH0vfNhjPN2t7Nh8G1hbAhFmppkx1T4vtjmY9vn9Y0ffxZN0G1rY2F0b2x8iZ45HZJ9tZ8P0ffPny6XZ-Xq6-n58uOqNFxKVhopHRGSGWDacdtCHq0E6zRUhgIw0TSNA4bb2lFiOWAjALggmknIkh2i93PcbRyuJ5tG1fl0W6ju7TAlVRMKrCZQZ3n0X0lyYMmgxizTt3_RzTDFPr8jK85rwLmErD7MysQhpWid2kbf6bhTBKvbvqjcF3XXl2zf7CNOTWfbB3nfiAze7YFORgcX8y_69OiEYIzLKruT2d34YHf_zqjOL5b3qcv5hk-j_f1wQ8dfCipWCXX15VTR9bfL9ao6U2v2B5c1skY</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Eruslanov, Evgeniy</creator><creator>Neuberger, Molly</creator><creator>Daurkin, Irina</creator><creator>Perrin, George Q.</creator><creator>Algood, Chester</creator><creator>Dahm, Philipp</creator><creator>Rosser, Charles</creator><creator>Vieweg, Johannes</creator><creator>Gilbert, Scott M.</creator><creator>Kusmartsev, Sergei</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20120301</creationdate><title>Circulating and tumor-infiltrating myeloid cell subsets in patients with bladder cancer</title><author>Eruslanov, Evgeniy ; Neuberger, Molly ; Daurkin, Irina ; Perrin, George Q. ; Algood, Chester ; Dahm, Philipp ; Rosser, Charles ; Vieweg, Johannes ; Gilbert, Scott M. ; Kusmartsev, Sergei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4883-c88f1583c63af4ed64edd86efa67c26635bbbf630d9f21e460c566451a386dd83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antigens, CD - metabolism</topic><topic>Antigens, Differentiation, Myelomonocytic - metabolism</topic><topic>Biological and medical sciences</topic><topic>Bladder cancer</topic><topic>Bone marrow</topic><topic>Cancer</topic><topic>CD11 Antigens - metabolism</topic><topic>Chemokines</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Granulocytes - immunology</topic><topic>Humans</topic><topic>immune suppresision</topic><topic>Immune Tolerance</topic><topic>inflammation</topic><topic>Lewis X Antigen - metabolism</topic><topic>Lymphocyte Activation</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Monocytes - immunology</topic><topic>myeloid cells</topic><topic>Myeloid Cells - immunology</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Sialic Acid Binding Ig-like Lectin 3</topic><topic>tumor-infiltrating myeloid cells</topic><topic>Tumors</topic><topic>Tumors of the urinary system</topic><topic>Urinary Bladder Neoplasms - immunology</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eruslanov, Evgeniy</creatorcontrib><creatorcontrib>Neuberger, Molly</creatorcontrib><creatorcontrib>Daurkin, Irina</creatorcontrib><creatorcontrib>Perrin, George Q.</creatorcontrib><creatorcontrib>Algood, Chester</creatorcontrib><creatorcontrib>Dahm, Philipp</creatorcontrib><creatorcontrib>Rosser, Charles</creatorcontrib><creatorcontrib>Vieweg, Johannes</creatorcontrib><creatorcontrib>Gilbert, Scott M.</creatorcontrib><creatorcontrib>Kusmartsev, Sergei</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eruslanov, Evgeniy</au><au>Neuberger, Molly</au><au>Daurkin, Irina</au><au>Perrin, George Q.</au><au>Algood, Chester</au><au>Dahm, Philipp</au><au>Rosser, Charles</au><au>Vieweg, Johannes</au><au>Gilbert, Scott M.</au><au>Kusmartsev, Sergei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating and tumor-infiltrating myeloid cell subsets in patients with bladder cancer</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int. J. Cancer</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>130</volume><issue>5</issue><spage>1109</spage><epage>1119</epage><pages>1109-1119</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>Both cancer‐related inflammation and tumor‐induced immune suppression are associated with expansion of myeloid cell subsets including myeloid‐derived suppressor cells. However, little known regarding characteristics of myeloid cells in patients with bladder cancer. In this study, we analyzed myeloid cells from peripheral blood (PBMC) and tumor tissue that were collected from patients with superficial noninvasive and invasive urothelial carcinomas. Our results demonstrate that PBMC from bladder cancer patients contain two major CD11b myeloid cell subsets: granulocyte‐type CD15high CD33low cells and monocyte‐type CD15low CD33high cells. The number of circulating granulocytic but not monocytic myeloid cells in cancer patients was markedly increased when compared to healthy individuals. Both myeloid cell subsets from cancer patients were highly activated and produced substantial amounts of proinflammatory chemokines/cytokines including CCL2, CCL3, CCL4, G‐CSF, IL‐8 and IL‐6. Granulocytic myeloid cells were able to inhibit in vitro T cell proliferation through induction of CD4+Foxp3+ T regulatory cells. Analysis of bladder cancer tissues revealed that tumors were infiltrated with monocyte–macrophage CD11b+HLA‐DR+ and granulocytic CD11b+CD15+HLA‐DR‐ myeloid cells. Collectively, this study identifies myeloid cell subsets in patients with bladder cancer. We demonstrate that these highly activated inflammatory myeloid cells represent a source of multiple chemokines/cytokines and may contribute to inflammation and immune dysfunction in bladder cancer.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>21480223</pmid><doi>10.1002/ijc.26123</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens, CD - metabolism Antigens, Differentiation, Myelomonocytic - metabolism Biological and medical sciences Bladder cancer Bone marrow Cancer CD11 Antigens - metabolism Chemokines Cytokines Cytokines - metabolism Granulocytes - immunology Humans immune suppresision Immune Tolerance inflammation Lewis X Antigen - metabolism Lymphocyte Activation Medical research Medical sciences Monocytes - immunology myeloid cells Myeloid Cells - immunology Nephrology. Urinary tract diseases Sialic Acid Binding Ig-like Lectin 3 tumor-infiltrating myeloid cells Tumors Tumors of the urinary system Urinary Bladder Neoplasms - immunology Urinary tract. Prostate gland |
| title | Circulating and tumor-infiltrating myeloid cell subsets in patients with bladder cancer |
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