Hyperbaric oxygen therapy in a mouse model of implant-associated osteomyelitis
Implant associated osteomyelitis (OM) is difficult to treat with antibiotics, and outcomes remain poor. Some reports suggest that hyperbaric oxygen treatment is a safe and effective means of treating OM. We tested this hypothesis in a murine model. Clinical isolates of methicillin‐resistant Staphylo...
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| Veröffentlicht in: | Journal of orthopaedic research 2012-02, Vol.30 (2), p.203-208 |
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| creator | Shandley, Sabrina Matthews, Krista Prato Cox, Jennifer Romano, Desiree Abplanalp, Allison Kalns, John |
| description | Implant associated osteomyelitis (OM) is difficult to treat with antibiotics, and outcomes remain poor. Some reports suggest that hyperbaric oxygen treatment is a safe and effective means of treating OM. We tested this hypothesis in a murine model. Clinical isolates of methicillin‐resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Klebsiella pneumoniae were used. The mice were infected with each of the three pathogens, treated with 100% oxygen at high pressure, hyperbaric oxygen (HBO), and monitored for the ability of HBO to prevent and/or clear the OM infection. Assessments included bacterial burden of the tibias and lesion scores, as well as receptor activator of NF‐κB ligand (RANKL) and myeloperoxidase (MPO) concentrations. HBO resulted in more severe lesion scores and higher RANKL and MPO concentrations for MRSA. A significant positive correlation was found between RANKL concentration and lesion score. No significant difference was found with HBO in P. aeruginosa infections and K. pneumoniae seems to either not infect bone well or get cleared before establishing an infection. The model is useful for studying OM infections caused by MRSA and P. aeruginosa, but HBO does not appear to be an efficacious treatment of an implant‐associated OM infection. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:203–208, 2012 |
| doi_str_mv | 10.1002/jor.21522 |
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Some reports suggest that hyperbaric oxygen treatment is a safe and effective means of treating OM. We tested this hypothesis in a murine model. Clinical isolates of methicillin‐resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Klebsiella pneumoniae were used. The mice were infected with each of the three pathogens, treated with 100% oxygen at high pressure, hyperbaric oxygen (HBO), and monitored for the ability of HBO to prevent and/or clear the OM infection. Assessments included bacterial burden of the tibias and lesion scores, as well as receptor activator of NF‐κB ligand (RANKL) and myeloperoxidase (MPO) concentrations. HBO resulted in more severe lesion scores and higher RANKL and MPO concentrations for MRSA. A significant positive correlation was found between RANKL concentration and lesion score. No significant difference was found with HBO in P. aeruginosa infections and K. pneumoniae seems to either not infect bone well or get cleared before establishing an infection. The model is useful for studying OM infections caused by MRSA and P. aeruginosa, but HBO does not appear to be an efficacious treatment of an implant‐associated OM infection. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:203–208, 2012</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1002/jor.21522</identifier><identifier>PMID: 21815206</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Disease Models, Animal ; hyperbaric oxygen ; Hyperbaric Oxygenation ; Klebsiella Infections - therapy ; Male ; Methicillin-Resistant Staphylococcus aureus ; Mice ; Mice, Inbred C57BL ; myeloperoxidase ; osteomyelitis ; Osteomyelitis - therapy ; Peroxidase - physiology ; Prostheses and Implants - adverse effects ; Pseudomonas Infections - therapy ; RANK Ligand - physiology ; RANKL ; Staphylococcal Infections - therapy ; Tibia - surgery</subject><ispartof>Journal of orthopaedic research, 2012-02, Vol.30 (2), p.203-208</ispartof><rights>Published 2011 Orthopaedic Research Society. 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Orthop. Res</addtitle><description>Implant associated osteomyelitis (OM) is difficult to treat with antibiotics, and outcomes remain poor. Some reports suggest that hyperbaric oxygen treatment is a safe and effective means of treating OM. We tested this hypothesis in a murine model. Clinical isolates of methicillin‐resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Klebsiella pneumoniae were used. The mice were infected with each of the three pathogens, treated with 100% oxygen at high pressure, hyperbaric oxygen (HBO), and monitored for the ability of HBO to prevent and/or clear the OM infection. Assessments included bacterial burden of the tibias and lesion scores, as well as receptor activator of NF‐κB ligand (RANKL) and myeloperoxidase (MPO) concentrations. HBO resulted in more severe lesion scores and higher RANKL and MPO concentrations for MRSA. A significant positive correlation was found between RANKL concentration and lesion score. No significant difference was found with HBO in P. aeruginosa infections and K. pneumoniae seems to either not infect bone well or get cleared before establishing an infection. The model is useful for studying OM infections caused by MRSA and P. aeruginosa, but HBO does not appear to be an efficacious treatment of an implant‐associated OM infection. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:203–208, 2012</description><subject>Animals</subject><subject>Disease Models, Animal</subject><subject>hyperbaric oxygen</subject><subject>Hyperbaric Oxygenation</subject><subject>Klebsiella Infections - therapy</subject><subject>Male</subject><subject>Methicillin-Resistant Staphylococcus aureus</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>myeloperoxidase</subject><subject>osteomyelitis</subject><subject>Osteomyelitis - therapy</subject><subject>Peroxidase - physiology</subject><subject>Prostheses and Implants - adverse effects</subject><subject>Pseudomonas Infections - therapy</subject><subject>RANK Ligand - physiology</subject><subject>RANKL</subject><subject>Staphylococcal Infections - therapy</subject><subject>Tibia - surgery</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtOwzAQRS0EgvJY8AMoO8QidOzEcbJEFbSgqkg8CjvLTSZgSOpgp4L8PYaU7tjMbM69ujqEHFM4pwBs-GbsOaOcsS0yoJzHIWfieZsMQERJCCxJ9si-c28AIChLd8keo6nHIRmQ2aRr0C6U1XlgvroXXAbtK1rVdIFeBiqozcqhvwVWgSkDXTeVWrahcs7kWrVYBMa1aOoOK91qd0h2SlU5PFr_A_J4dfkwmoTT2_H16GIa5lEmWEjzLFdIEVUmRAqF4EWiRCYKUbDUrwbOkUdccMEA4jxbCKC8jKNFosqiFFl0QE773saajxW6Vtba5Vj5cegXy4wyCmkccU-e9WRujXMWS9lYXSvbSQryx5709uSvPc-erFtXixqLDfmnywPDHvjUFXb_N8mb27u_yrBPaK_pa5NQ9l0mIhJcPs3Gks6n85v70VxC9A2Tk4hr</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Shandley, Sabrina</creator><creator>Matthews, Krista Prato</creator><creator>Cox, Jennifer</creator><creator>Romano, Desiree</creator><creator>Abplanalp, Allison</creator><creator>Kalns, John</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201202</creationdate><title>Hyperbaric oxygen therapy in a mouse model of implant-associated osteomyelitis</title><author>Shandley, Sabrina ; Matthews, Krista Prato ; Cox, Jennifer ; Romano, Desiree ; Abplanalp, Allison ; Kalns, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3972-1c9cae1eea97780d75d6a797d7d28073055e5357572004c9b7015f43b6afdf793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Disease Models, Animal</topic><topic>hyperbaric oxygen</topic><topic>Hyperbaric Oxygenation</topic><topic>Klebsiella Infections - therapy</topic><topic>Male</topic><topic>Methicillin-Resistant Staphylococcus aureus</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>myeloperoxidase</topic><topic>osteomyelitis</topic><topic>Osteomyelitis - therapy</topic><topic>Peroxidase - physiology</topic><topic>Prostheses and Implants - adverse effects</topic><topic>Pseudomonas Infections - therapy</topic><topic>RANK Ligand - physiology</topic><topic>RANKL</topic><topic>Staphylococcal Infections - therapy</topic><topic>Tibia - surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shandley, Sabrina</creatorcontrib><creatorcontrib>Matthews, Krista Prato</creatorcontrib><creatorcontrib>Cox, Jennifer</creatorcontrib><creatorcontrib>Romano, Desiree</creatorcontrib><creatorcontrib>Abplanalp, Allison</creatorcontrib><creatorcontrib>Kalns, John</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shandley, Sabrina</au><au>Matthews, Krista Prato</au><au>Cox, Jennifer</au><au>Romano, Desiree</au><au>Abplanalp, Allison</au><au>Kalns, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hyperbaric oxygen therapy in a mouse model of implant-associated osteomyelitis</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J. Orthop. Res</addtitle><date>2012-02</date><risdate>2012</risdate><volume>30</volume><issue>2</issue><spage>203</spage><epage>208</epage><pages>203-208</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><abstract>Implant associated osteomyelitis (OM) is difficult to treat with antibiotics, and outcomes remain poor. Some reports suggest that hyperbaric oxygen treatment is a safe and effective means of treating OM. We tested this hypothesis in a murine model. Clinical isolates of methicillin‐resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Klebsiella pneumoniae were used. The mice were infected with each of the three pathogens, treated with 100% oxygen at high pressure, hyperbaric oxygen (HBO), and monitored for the ability of HBO to prevent and/or clear the OM infection. Assessments included bacterial burden of the tibias and lesion scores, as well as receptor activator of NF‐κB ligand (RANKL) and myeloperoxidase (MPO) concentrations. 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| subjects | Animals Disease Models, Animal hyperbaric oxygen Hyperbaric Oxygenation Klebsiella Infections - therapy Male Methicillin-Resistant Staphylococcus aureus Mice Mice, Inbred C57BL myeloperoxidase osteomyelitis Osteomyelitis - therapy Peroxidase - physiology Prostheses and Implants - adverse effects Pseudomonas Infections - therapy RANK Ligand - physiology RANKL Staphylococcal Infections - therapy Tibia - surgery |
| title | Hyperbaric oxygen therapy in a mouse model of implant-associated osteomyelitis |
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