Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco
Extended-spectrum beta-lactamase- (ESBL)-producing Escherichia coli are an increasingly significant cause of community-acquired infection worldwide. The aim of this study was to assess the prevalence of ESBL-producing E. coli in a community, to analyze the relationship between strains studied, and t...
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| Veröffentlicht in: | Journal of infection in developing countries 2011-12, Vol.5 (12), p.850-855 |
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| creator | Bourjilat, Fatna Bouchrif, Brahim Dersi, Noureddine Claude, Jean David Perrier Gros Amarouch, Hamid Timinouni, Mohammed |
| description | Extended-spectrum beta-lactamase- (ESBL)-producing Escherichia coli are an increasingly significant cause of community-acquired infection worldwide. The aim of this study was to assess the prevalence of ESBL-producing E. coli in a community, to analyze the relationship between strains studied, and to characterize the ESBL genes involved in this resistance.
ESBL production was detected by the double disk synergy test. Genes encoding ESBLs (blaTEM, blaCTM, blaSHV) were identified by PCR and DNA sequencing. Conjugation experiments were performed to check the transferability of antibiotic resistance genes. Strain inter-relationships were studied by pulsed field gel electrophoresis.
Seven ESBL-producing E. coli were identified among the 535 E. coli isolates. Most of them expressed a CTX-M enzyme (6/7) with a predominance of CTX-M-15 (6/6). Two strains possessed TEM in combination with CTX-M-15 or SHV-5. Plasmid content and gene transfer analysis showed that resistance genes were carried by high molecular weight conjugative plasmids. PFGE analysis showed that the strains were not clonal.
ESBL-producing E. coli from urinary tract infections in Casablanca belong to different clones and carry mobile beta-lactamase genes. It is therefore essential to monitor the epidemiology of ESBLs in E. coli and related organisms locally to effectively combat resistance. |
| doi_str_mv | 10.3855/jidc.1490 |
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ESBL production was detected by the double disk synergy test. Genes encoding ESBLs (blaTEM, blaCTM, blaSHV) were identified by PCR and DNA sequencing. Conjugation experiments were performed to check the transferability of antibiotic resistance genes. Strain inter-relationships were studied by pulsed field gel electrophoresis.
Seven ESBL-producing E. coli were identified among the 535 E. coli isolates. Most of them expressed a CTX-M enzyme (6/7) with a predominance of CTX-M-15 (6/6). Two strains possessed TEM in combination with CTX-M-15 or SHV-5. Plasmid content and gene transfer analysis showed that resistance genes were carried by high molecular weight conjugative plasmids. PFGE analysis showed that the strains were not clonal.
ESBL-producing E. coli from urinary tract infections in Casablanca belong to different clones and carry mobile beta-lactamase genes. It is therefore essential to monitor the epidemiology of ESBLs in E. coli and related organisms locally to effectively combat resistance.</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.1490</identifier><identifier>PMID: 22169783</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>beta-Lactamases - secretion ; Cluster Analysis ; Community-Acquired Infections - epidemiology ; Community-Acquired Infections - microbiology ; Conjugation, Genetic ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; E coli ; Electrophoresis, Gel, Pulsed-Field ; Escherichia coli - enzymology ; Escherichia coli - isolation & purification ; Escherichia coli Infections - epidemiology ; Escherichia coli Infections - microbiology ; Escherichia coli Proteins - genetics ; Gene Transfer, Horizontal ; Genotype ; Humans ; Molecular Epidemiology ; Molecular Typing ; Morocco - epidemiology ; Plasmids - analysis ; Polymerase Chain Reaction ; Prevalence ; Sequence Analysis, DNA ; Urinary tract infections</subject><ispartof>Journal of infection in developing countries, 2011-12, Vol.5 (12), p.850-855</ispartof><rights>2011. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1573-c380da3078cdd5e900a5bfb25c6c83399b1e1221e824e9ddca6e646a11da948c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22169783$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bourjilat, Fatna</creatorcontrib><creatorcontrib>Bouchrif, Brahim</creatorcontrib><creatorcontrib>Dersi, Noureddine</creatorcontrib><creatorcontrib>Claude, Jean David Perrier Gros</creatorcontrib><creatorcontrib>Amarouch, Hamid</creatorcontrib><creatorcontrib>Timinouni, Mohammed</creatorcontrib><title>Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco</title><title>Journal of infection in developing countries</title><addtitle>J Infect Dev Ctries</addtitle><description>Extended-spectrum beta-lactamase- (ESBL)-producing Escherichia coli are an increasingly significant cause of community-acquired infection worldwide. The aim of this study was to assess the prevalence of ESBL-producing E. coli in a community, to analyze the relationship between strains studied, and to characterize the ESBL genes involved in this resistance.
ESBL production was detected by the double disk synergy test. Genes encoding ESBLs (blaTEM, blaCTM, blaSHV) were identified by PCR and DNA sequencing. Conjugation experiments were performed to check the transferability of antibiotic resistance genes. Strain inter-relationships were studied by pulsed field gel electrophoresis.
Seven ESBL-producing E. coli were identified among the 535 E. coli isolates. Most of them expressed a CTX-M enzyme (6/7) with a predominance of CTX-M-15 (6/6). Two strains possessed TEM in combination with CTX-M-15 or SHV-5. Plasmid content and gene transfer analysis showed that resistance genes were carried by high molecular weight conjugative plasmids. PFGE analysis showed that the strains were not clonal.
ESBL-producing E. coli from urinary tract infections in Casablanca belong to different clones and carry mobile beta-lactamase genes. It is therefore essential to monitor the epidemiology of ESBLs in E. coli and related organisms locally to effectively combat resistance.</description><subject>beta-Lactamases - secretion</subject><subject>Cluster Analysis</subject><subject>Community-Acquired Infections - epidemiology</subject><subject>Community-Acquired Infections - microbiology</subject><subject>Conjugation, Genetic</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>E coli</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli Infections - epidemiology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Gene Transfer, Horizontal</subject><subject>Genotype</subject><subject>Humans</subject><subject>Molecular Epidemiology</subject><subject>Molecular Typing</subject><subject>Morocco - epidemiology</subject><subject>Plasmids - analysis</subject><subject>Polymerase Chain Reaction</subject><subject>Prevalence</subject><subject>Sequence Analysis, DNA</subject><subject>Urinary tract infections</subject><issn>1972-2680</issn><issn>2036-6590</issn><issn>1972-2680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkU9rFTEUxYNYbK0u_AIy4EIEp-bPZF6ylMdTCy3d6Hq4c3OnzWMmeU0mYD-C39o8WkVcnQv3x-EcDmNvBL9QRutPe-_wQnSWP2Nnwm5kK3vDn_9zn7KXOe8511Zp8YKdSil6uzHqjP3aLZRuKSA1cWro50rBkWvzgXBNZWlGWqGdAVdYIFNuDym6gj7cNruMd5Q83nloMM6-8aHqspTg14cW8L74RK4pyQdID_U7VUsfQz6CW8gwzhAQPjbXMUXE-IqdTDBnev2k5-zHl9337bf26ubr5fbzVYtCb1SLynAHim8MOqfJcg56nEapsUejlLWjIFH7kZEdWecQeuq7HoRwYDuD6py9f_StVe4L5XVYfEaaaxqKJQ9WCNvpjstKvvuP3MeSQg03SN1zaUynRKU-PFKYYs6JpuGQ_FIrD4IPx3mG4zzDcZ7Kvn1yLONC7i_5Zw_1G8jgjYY</recordid><startdate>20111213</startdate><enddate>20111213</enddate><creator>Bourjilat, Fatna</creator><creator>Bouchrif, Brahim</creator><creator>Dersi, Noureddine</creator><creator>Claude, Jean David Perrier Gros</creator><creator>Amarouch, Hamid</creator><creator>Timinouni, Mohammed</creator><general>Journal of Infection in Developing Countries</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8C1</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20111213</creationdate><title>Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco</title><author>Bourjilat, Fatna ; 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The aim of this study was to assess the prevalence of ESBL-producing E. coli in a community, to analyze the relationship between strains studied, and to characterize the ESBL genes involved in this resistance.
ESBL production was detected by the double disk synergy test. Genes encoding ESBLs (blaTEM, blaCTM, blaSHV) were identified by PCR and DNA sequencing. Conjugation experiments were performed to check the transferability of antibiotic resistance genes. Strain inter-relationships were studied by pulsed field gel electrophoresis.
Seven ESBL-producing E. coli were identified among the 535 E. coli isolates. Most of them expressed a CTX-M enzyme (6/7) with a predominance of CTX-M-15 (6/6). Two strains possessed TEM in combination with CTX-M-15 or SHV-5. Plasmid content and gene transfer analysis showed that resistance genes were carried by high molecular weight conjugative plasmids. PFGE analysis showed that the strains were not clonal.
ESBL-producing E. coli from urinary tract infections in Casablanca belong to different clones and carry mobile beta-lactamase genes. It is therefore essential to monitor the epidemiology of ESBLs in E. coli and related organisms locally to effectively combat resistance.</abstract><cop>Italy</cop><pub>Journal of Infection in Developing Countries</pub><pmid>22169783</pmid><doi>10.3855/jidc.1490</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1972-2680 |
| ispartof | Journal of infection in developing countries, 2011-12, Vol.5 (12), p.850-855 |
| issn | 1972-2680 2036-6590 1972-2680 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | beta-Lactamases - secretion Cluster Analysis Community-Acquired Infections - epidemiology Community-Acquired Infections - microbiology Conjugation, Genetic DNA, Bacterial - chemistry DNA, Bacterial - genetics E coli Electrophoresis, Gel, Pulsed-Field Escherichia coli - enzymology Escherichia coli - isolation & purification Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics Gene Transfer, Horizontal Genotype Humans Molecular Epidemiology Molecular Typing Morocco - epidemiology Plasmids - analysis Polymerase Chain Reaction Prevalence Sequence Analysis, DNA Urinary tract infections |
| title | Emergence of extended-spectrum beta-lactamases-producing Escherichia coli in community-acquired urinary infections in Casablanca, Morocco |
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