Specific delivery of AtBT1 to mitochondria complements the aberrant growth and sterility phenotype of homozygous Atbt1 Arabidopsis mutants
Summary It has been shown that homozygous AtBT1::T‐DNA Arabidopsis mutants display an aberrant growth and sterility phenotype, and that AtBT1 is a carrier that is exclusively localized to the inner plastidial envelope and is required for export of newly synthesized adenylates into the cytosol. Howev...
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| creator | Bahaji, Abdellatif Muñoz, Francisco José Ovecka, Miroslav Baroja‐Fernández, Edurne Montero, Manuel Li, Jun Hidalgo, Maite Almagro, Goizeder Sesma, María Teresa Ezquer, Ignacio Pozueta‐Romero, Javier |
| description | Summary
It has been shown that homozygous AtBT1::T‐DNA Arabidopsis mutants display an aberrant growth and sterility phenotype, and that AtBT1 is a carrier that is exclusively localized to the inner plastidial envelope and is required for export of newly synthesized adenylates into the cytosol. However, a recent demonstration that AtBT1 is localized to both plastids and mitochondria suggested that plastidic AtBT1 is not necessary for normal growth and fertility of Arabidopsis. To test this hypothesis, we produced and characterized homozygous AtBT1::T‐DNA mutants stably expressing either dually localized AtBT1 or AtBT1 specifically localized to the mitochondrial compartment. These analyses revealed that the aberrant growth and sterility phenotype of homozygous AtBT1::T‐DNA mutants was complemented when expressing both the dual‐targeted AtBT1 and AtBT1 specifically delivered to mitochondria. These data confirm that (i) plastidic AtBT1 is not strictly required for normal growth and fertility of the plant, and (ii) specific delivery of AtBT1 to mitochondria is enough to complement the aberrant growth and sterility phenotype of homozygous AtBT1::T‐DNA mutants. Furthermore, data presented here question the idea that the requirement for AtBT1 is due to its involvement in transport of newly synthesized adenylates from the plastid to the cytosol, and suggest that the protein may play as yet unidentified functions in plastids and mitochondria. |
| doi_str_mv | 10.1111/j.1365-313X.2011.04767.x |
| format | Article |
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It has been shown that homozygous AtBT1::T‐DNA Arabidopsis mutants display an aberrant growth and sterility phenotype, and that AtBT1 is a carrier that is exclusively localized to the inner plastidial envelope and is required for export of newly synthesized adenylates into the cytosol. However, a recent demonstration that AtBT1 is localized to both plastids and mitochondria suggested that plastidic AtBT1 is not necessary for normal growth and fertility of Arabidopsis. To test this hypothesis, we produced and characterized homozygous AtBT1::T‐DNA mutants stably expressing either dually localized AtBT1 or AtBT1 specifically localized to the mitochondrial compartment. These analyses revealed that the aberrant growth and sterility phenotype of homozygous AtBT1::T‐DNA mutants was complemented when expressing both the dual‐targeted AtBT1 and AtBT1 specifically delivered to mitochondria. These data confirm that (i) plastidic AtBT1 is not strictly required for normal growth and fertility of the plant, and (ii) specific delivery of AtBT1 to mitochondria is enough to complement the aberrant growth and sterility phenotype of homozygous AtBT1::T‐DNA mutants. Furthermore, data presented here question the idea that the requirement for AtBT1 is due to its involvement in transport of newly synthesized adenylates from the plastid to the cytosol, and suggest that the protein may play as yet unidentified functions in plastids and mitochondria.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313X.2011.04767.x</identifier><identifier>PMID: 21883554</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Biological and medical sciences ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Cytosol - metabolism ; DNA, Bacterial ; dual targeting ; fertility ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; Genes, Plant ; Genotype & phenotype ; Homozygote ; Mitochondria ; Mitochondria - genetics ; Mitochondria - metabolism ; mitochondrial carrier family ; Molecular and cellular biology ; Mutation ; Phenotype ; Plant biology ; plant development ; Plant Infertility - genetics ; Plant physiology and development ; Plastids ; Plastids - genetics ; Plastids - metabolism ; protein function ; starch</subject><ispartof>The Plant journal : for cell and molecular biology, 2011-12, Vol.68 (6), p.1115-1121</ispartof><rights>2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4257-7e731c344ef45785e6994a75039d8954364d3d8d731fa7fad326defef31bdf753</citedby><cites>FETCH-LOGICAL-c4257-7e731c344ef45785e6994a75039d8954364d3d8d731fa7fad326defef31bdf753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2011.04767.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2011.04767.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25292479$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21883554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bahaji, Abdellatif</creatorcontrib><creatorcontrib>Muñoz, Francisco José</creatorcontrib><creatorcontrib>Ovecka, Miroslav</creatorcontrib><creatorcontrib>Baroja‐Fernández, Edurne</creatorcontrib><creatorcontrib>Montero, Manuel</creatorcontrib><creatorcontrib>Li, Jun</creatorcontrib><creatorcontrib>Hidalgo, Maite</creatorcontrib><creatorcontrib>Almagro, Goizeder</creatorcontrib><creatorcontrib>Sesma, María Teresa</creatorcontrib><creatorcontrib>Ezquer, Ignacio</creatorcontrib><creatorcontrib>Pozueta‐Romero, Javier</creatorcontrib><title>Specific delivery of AtBT1 to mitochondria complements the aberrant growth and sterility phenotype of homozygous Atbt1 Arabidopsis mutants</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Summary
It has been shown that homozygous AtBT1::T‐DNA Arabidopsis mutants display an aberrant growth and sterility phenotype, and that AtBT1 is a carrier that is exclusively localized to the inner plastidial envelope and is required for export of newly synthesized adenylates into the cytosol. However, a recent demonstration that AtBT1 is localized to both plastids and mitochondria suggested that plastidic AtBT1 is not necessary for normal growth and fertility of Arabidopsis. To test this hypothesis, we produced and characterized homozygous AtBT1::T‐DNA mutants stably expressing either dually localized AtBT1 or AtBT1 specifically localized to the mitochondrial compartment. These analyses revealed that the aberrant growth and sterility phenotype of homozygous AtBT1::T‐DNA mutants was complemented when expressing both the dual‐targeted AtBT1 and AtBT1 specifically delivered to mitochondria. These data confirm that (i) plastidic AtBT1 is not strictly required for normal growth and fertility of the plant, and (ii) specific delivery of AtBT1 to mitochondria is enough to complement the aberrant growth and sterility phenotype of homozygous AtBT1::T‐DNA mutants. Furthermore, data presented here question the idea that the requirement for AtBT1 is due to its involvement in transport of newly synthesized adenylates from the plastid to the cytosol, and suggest that the protein may play as yet unidentified functions in plastids and mitochondria.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Cytosol - metabolism</subject><subject>DNA, Bacterial</subject><subject>dual targeting</subject><subject>fertility</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant</subject><subject>Genotype & phenotype</subject><subject>Homozygote</subject><subject>Mitochondria</subject><subject>Mitochondria - genetics</subject><subject>Mitochondria - metabolism</subject><subject>mitochondrial carrier family</subject><subject>Molecular and cellular biology</subject><subject>Mutation</subject><subject>Phenotype</subject><subject>Plant biology</subject><subject>plant development</subject><subject>Plant Infertility - genetics</subject><subject>Plant physiology and development</subject><subject>Plastids</subject><subject>Plastids - genetics</subject><subject>Plastids - metabolism</subject><subject>protein function</subject><subject>starch</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd-O1CAUhxujccfVVzDExHjVCgVKe-HF7Ma_2UQTx8Q7Qsthh0lbKlB36yP41FJnXBOv5AYSvvM7B74sQwQXJK2Xh4LQiueU0K9FiQkpMBOVKG7vZZu7i_vZBjcVzgUj5Vn2KIQDxkTQij3MzkpS15Rztsl-fp6gs8Z2SENvv4NfkDNoGy92BEWHBhtdt3ej9lahzg1TDwOMMaC4B6Ra8F6NEV17dxP3SI0ahQje9jYuaNrD6OIywRq4d4P7sVy7OaTsNhK09aq12k3BBjTMMaWEx9kDo_oAT077efblzevd5bv86uPb95fbq7xjJRe5AEFJRxkDw7ioOVRNw5TgmDa6bjhLL9RU1zpRRgmjNC0rDQYMJa02gtPz7MUxd_Lu2wwhysGGDvpejZAGlA0hTepEVvLZP-TBzX5MwyUIM8YZEwmqj1DnXQgejJy8HZRfJMFytSUPcpUiVylytSV_25K3qfTpKX9uB9B3hX_0JOD5CVChU71Jv93Z8JfjZVMy0STu1ZG7sT0s_z2A3H36sJ7oL1fuspc</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Bahaji, Abdellatif</creator><creator>Muñoz, Francisco José</creator><creator>Ovecka, Miroslav</creator><creator>Baroja‐Fernández, Edurne</creator><creator>Montero, Manuel</creator><creator>Li, Jun</creator><creator>Hidalgo, Maite</creator><creator>Almagro, Goizeder</creator><creator>Sesma, María Teresa</creator><creator>Ezquer, Ignacio</creator><creator>Pozueta‐Romero, Javier</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201112</creationdate><title>Specific delivery of AtBT1 to mitochondria complements the aberrant growth and sterility phenotype of homozygous Atbt1 Arabidopsis mutants</title><author>Bahaji, Abdellatif ; Muñoz, Francisco José ; Ovecka, Miroslav ; Baroja‐Fernández, Edurne ; Montero, Manuel ; Li, Jun ; Hidalgo, Maite ; Almagro, Goizeder ; Sesma, María Teresa ; Ezquer, Ignacio ; Pozueta‐Romero, Javier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4257-7e731c344ef45785e6994a75039d8954364d3d8d731fa7fad326defef31bdf753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Cytosol - metabolism</topic><topic>DNA, Bacterial</topic><topic>dual targeting</topic><topic>fertility</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes, Plant</topic><topic>Genotype & phenotype</topic><topic>Homozygote</topic><topic>Mitochondria</topic><topic>Mitochondria - genetics</topic><topic>Mitochondria - metabolism</topic><topic>mitochondrial carrier family</topic><topic>Molecular and cellular biology</topic><topic>Mutation</topic><topic>Phenotype</topic><topic>Plant biology</topic><topic>plant development</topic><topic>Plant Infertility - genetics</topic><topic>Plant physiology and development</topic><topic>Plastids</topic><topic>Plastids - genetics</topic><topic>Plastids - metabolism</topic><topic>protein function</topic><topic>starch</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bahaji, Abdellatif</creatorcontrib><creatorcontrib>Muñoz, Francisco José</creatorcontrib><creatorcontrib>Ovecka, Miroslav</creatorcontrib><creatorcontrib>Baroja‐Fernández, Edurne</creatorcontrib><creatorcontrib>Montero, Manuel</creatorcontrib><creatorcontrib>Li, Jun</creatorcontrib><creatorcontrib>Hidalgo, Maite</creatorcontrib><creatorcontrib>Almagro, Goizeder</creatorcontrib><creatorcontrib>Sesma, María Teresa</creatorcontrib><creatorcontrib>Ezquer, Ignacio</creatorcontrib><creatorcontrib>Pozueta‐Romero, Javier</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bahaji, Abdellatif</au><au>Muñoz, Francisco José</au><au>Ovecka, Miroslav</au><au>Baroja‐Fernández, Edurne</au><au>Montero, Manuel</au><au>Li, Jun</au><au>Hidalgo, Maite</au><au>Almagro, Goizeder</au><au>Sesma, María Teresa</au><au>Ezquer, Ignacio</au><au>Pozueta‐Romero, Javier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific delivery of AtBT1 to mitochondria complements the aberrant growth and sterility phenotype of homozygous Atbt1 Arabidopsis mutants</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2011-12</date><risdate>2011</risdate><volume>68</volume><issue>6</issue><spage>1115</spage><epage>1121</epage><pages>1115-1121</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Summary
It has been shown that homozygous AtBT1::T‐DNA Arabidopsis mutants display an aberrant growth and sterility phenotype, and that AtBT1 is a carrier that is exclusively localized to the inner plastidial envelope and is required for export of newly synthesized adenylates into the cytosol. However, a recent demonstration that AtBT1 is localized to both plastids and mitochondria suggested that plastidic AtBT1 is not necessary for normal growth and fertility of Arabidopsis. To test this hypothesis, we produced and characterized homozygous AtBT1::T‐DNA mutants stably expressing either dually localized AtBT1 or AtBT1 specifically localized to the mitochondrial compartment. These analyses revealed that the aberrant growth and sterility phenotype of homozygous AtBT1::T‐DNA mutants was complemented when expressing both the dual‐targeted AtBT1 and AtBT1 specifically delivered to mitochondria. These data confirm that (i) plastidic AtBT1 is not strictly required for normal growth and fertility of the plant, and (ii) specific delivery of AtBT1 to mitochondria is enough to complement the aberrant growth and sterility phenotype of homozygous AtBT1::T‐DNA mutants. Furthermore, data presented here question the idea that the requirement for AtBT1 is due to its involvement in transport of newly synthesized adenylates from the plastid to the cytosol, and suggest that the protein may play as yet unidentified functions in plastids and mitochondria.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21883554</pmid><doi>10.1111/j.1365-313X.2011.04767.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Biological and medical sciences Cell differentiation, maturation, development, hematopoiesis Cell physiology Cytosol - metabolism DNA, Bacterial dual targeting fertility Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Genes, Plant Genotype & phenotype Homozygote Mitochondria Mitochondria - genetics Mitochondria - metabolism mitochondrial carrier family Molecular and cellular biology Mutation Phenotype Plant biology plant development Plant Infertility - genetics Plant physiology and development Plastids Plastids - genetics Plastids - metabolism protein function starch |
| title | Specific delivery of AtBT1 to mitochondria complements the aberrant growth and sterility phenotype of homozygous Atbt1 Arabidopsis mutants |
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