An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis
In plants, osmotic stress-responsive transcriptional regulation depends mainly on two major classes of cis-acting elements found in the promoter regions of stress-inducible genes: ABA-responsive elements (ABREs) and dehydration-responsive elements (DREs). ABRE has been shown to perceive ABA-mediated...
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| creator | Kim, June-Sik Mizoi, Junya Yoshida, Takuya Fujita, Yasunari Nakajima, Jun Ohori, Teppei Todaka, Daisuke Nakashima, Kazuo Hirayama, Takashi Shinozaki, Kazuo Yamaguchi-Shinozaki, Kazuko |
| description | In plants, osmotic stress-responsive transcriptional regulation depends mainly on two major classes of cis-acting elements found in the promoter regions of stress-inducible genes: ABA-responsive elements (ABREs) and dehydration-responsive elements (DREs). ABRE has been shown to perceive ABA-mediated osmotic stress signals, whereas DRE is known to be involved in an ABA-independent pathway. Previously, we reported that the transcription factor DRE-BINDING PROTEIN 2A (DREB2A) regulates DRE-mediated transcription of target genes under osmotic stress conditions in Arabidopsis (Arabidopsis thaliana). However, the transcriptional regulation of DREB2A itself remains largely uncharacterized. To elucidate the transcriptional mechanism associated with the DREB2A gene under osmotic stress conditions, we generated a series of truncated and base-substituted variants of the DREB2A promoter and evaluated their transcriptional activities individually. We found that both ABRE and coupling element 3 (CE3)-like sequences located approximately −100 bp from the transcriptional initiation site are necessary for the dehydration-responsive expression of DREB2A. Coupling our transient expression analyses with yeast one-hybrid and chromatin immunoprecipitation (ChIP) assays indicated that the ABRE-BINDING PROTEIN 1 (AREB1), AREB2 and ABRE-BINDING FACTOR 3 (ABF3) bZIP transcription factors can bind to and activate the DREB2A promoter in an ABRE-dependent manner. Exogenous ABA application induced only a modest accumulation of the DREB2A transcript when compared with the osmotic stress treatment. However, the osmotic stress-induced DREB2A expression was found to be markedly impaired in several ABA-deficient and ABA-insensitive mutants. These results suggest that in addition to an ABA-independent pathway, the ABA-dependent pathway plays a positive role in the osmotic stress-responsive expression of DREB2A. |
| doi_str_mv | 10.1093/pcp/pcr143 |
| format | Article |
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ABRE has been shown to perceive ABA-mediated osmotic stress signals, whereas DRE is known to be involved in an ABA-independent pathway. Previously, we reported that the transcription factor DRE-BINDING PROTEIN 2A (DREB2A) regulates DRE-mediated transcription of target genes under osmotic stress conditions in Arabidopsis (Arabidopsis thaliana). However, the transcriptional regulation of DREB2A itself remains largely uncharacterized. To elucidate the transcriptional mechanism associated with the DREB2A gene under osmotic stress conditions, we generated a series of truncated and base-substituted variants of the DREB2A promoter and evaluated their transcriptional activities individually. We found that both ABRE and coupling element 3 (CE3)-like sequences located approximately −100 bp from the transcriptional initiation site are necessary for the dehydration-responsive expression of DREB2A. Coupling our transient expression analyses with yeast one-hybrid and chromatin immunoprecipitation (ChIP) assays indicated that the ABRE-BINDING PROTEIN 1 (AREB1), AREB2 and ABRE-BINDING FACTOR 3 (ABF3) bZIP transcription factors can bind to and activate the DREB2A promoter in an ABRE-dependent manner. Exogenous ABA application induced only a modest accumulation of the DREB2A transcript when compared with the osmotic stress treatment. However, the osmotic stress-induced DREB2A expression was found to be markedly impaired in several ABA-deficient and ABA-insensitive mutants. These results suggest that in addition to an ABA-independent pathway, the ABA-dependent pathway plays a positive role in the osmotic stress-responsive expression of DREB2A.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcr143</identifier><identifier>PMID: 22025559</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>Abscisic Acid - pharmacology ; Arabidopsis - genetics ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Base Sequence ; Droughts ; Gene Expression Regulation, Plant - drug effects ; Genes, Plant - genetics ; Models, Biological ; Molecular Sequence Data ; Mutation - genetics ; Osmosis - drug effects ; Protein Binding - drug effects ; Response Elements - genetics ; Sequence Deletion - genetics ; Stress, Physiological - drug effects ; Stress, Physiological - genetics ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcriptional Activation - drug effects ; Transcriptional Activation - genetics</subject><ispartof>Plant and cell physiology, 2011-12, Vol.52 (12), p.2136-2146</ispartof><rights>The Author 2011. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-1186a03dcb5c94fdb3b9ddc1a749152b691746af9541e5aaf9e5eff4b78a294a3</citedby><cites>FETCH-LOGICAL-c478t-1186a03dcb5c94fdb3b9ddc1a749152b691746af9541e5aaf9e5eff4b78a294a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22025559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, June-Sik</creatorcontrib><creatorcontrib>Mizoi, Junya</creatorcontrib><creatorcontrib>Yoshida, Takuya</creatorcontrib><creatorcontrib>Fujita, Yasunari</creatorcontrib><creatorcontrib>Nakajima, Jun</creatorcontrib><creatorcontrib>Ohori, Teppei</creatorcontrib><creatorcontrib>Todaka, Daisuke</creatorcontrib><creatorcontrib>Nakashima, Kazuo</creatorcontrib><creatorcontrib>Hirayama, Takashi</creatorcontrib><creatorcontrib>Shinozaki, Kazuo</creatorcontrib><creatorcontrib>Yamaguchi-Shinozaki, Kazuko</creatorcontrib><title>An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>In plants, osmotic stress-responsive transcriptional regulation depends mainly on two major classes of cis-acting elements found in the promoter regions of stress-inducible genes: ABA-responsive elements (ABREs) and dehydration-responsive elements (DREs). ABRE has been shown to perceive ABA-mediated osmotic stress signals, whereas DRE is known to be involved in an ABA-independent pathway. Previously, we reported that the transcription factor DRE-BINDING PROTEIN 2A (DREB2A) regulates DRE-mediated transcription of target genes under osmotic stress conditions in Arabidopsis (Arabidopsis thaliana). However, the transcriptional regulation of DREB2A itself remains largely uncharacterized. To elucidate the transcriptional mechanism associated with the DREB2A gene under osmotic stress conditions, we generated a series of truncated and base-substituted variants of the DREB2A promoter and evaluated their transcriptional activities individually. We found that both ABRE and coupling element 3 (CE3)-like sequences located approximately −100 bp from the transcriptional initiation site are necessary for the dehydration-responsive expression of DREB2A. Coupling our transient expression analyses with yeast one-hybrid and chromatin immunoprecipitation (ChIP) assays indicated that the ABRE-BINDING PROTEIN 1 (AREB1), AREB2 and ABRE-BINDING FACTOR 3 (ABF3) bZIP transcription factors can bind to and activate the DREB2A promoter in an ABRE-dependent manner. Exogenous ABA application induced only a modest accumulation of the DREB2A transcript when compared with the osmotic stress treatment. However, the osmotic stress-induced DREB2A expression was found to be markedly impaired in several ABA-deficient and ABA-insensitive mutants. These results suggest that in addition to an ABA-independent pathway, the ABA-dependent pathway plays a positive role in the osmotic stress-responsive expression of DREB2A.</description><subject>Abscisic Acid - pharmacology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Base Sequence</subject><subject>Droughts</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Genes, Plant - genetics</subject><subject>Models, Biological</subject><subject>Molecular Sequence Data</subject><subject>Mutation - genetics</subject><subject>Osmosis - drug effects</subject><subject>Protein Binding - drug effects</subject><subject>Response Elements - genetics</subject><subject>Sequence Deletion - genetics</subject><subject>Stress, Physiological - drug effects</subject><subject>Stress, Physiological - genetics</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcriptional Activation - drug effects</subject><subject>Transcriptional Activation - genetics</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EokvhwgOguSAQImDH8SY-pm1aVqpUtC3iGDnOZNcoa7t2soI34_HwsoVjD6MZzXzzazQ_Ia8Z_cSo5J-99ikCK_gTsmBFyTJJBX9KFpTyPKNlxU7Iixh_UJpqTp-TkzynuRBCLsjv2kJ9tm7ga3A7N2GAW7yf0WoEE2Fl927cYw_Gwk1Mc6PhdgoYY7bG6J2NZo_Q_PSHlnEW3ADTFuFi3ZzlNVyhxY_wfWv0FhqrXY8RFNwFZaMOxk-HjUulJxdgjZt5VJOxG7gIbt5sp2xl-1mbbsS_OvFwQx1UZ3rno4kvybNBjRFfPeRT8u2yuTv_kl3fXK3O6-tMF2U1ZYxVS0V5rzuhZTH0He9k32umykIykXdLycpiqQYpCoZCpQIFDkPRlZXKZaH4KXl31PXBpcfEqd2ZqHEclUU3x1YyJnmVC57I94-SjLJSLnNZyYR-OKI6uBgDDq0PZqfCrwS1B0_b5Gl79DTBbx50526H_X_0n4kJeHsE3OwfE_oDWSysfQ</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Kim, June-Sik</creator><creator>Mizoi, Junya</creator><creator>Yoshida, Takuya</creator><creator>Fujita, Yasunari</creator><creator>Nakajima, Jun</creator><creator>Ohori, Teppei</creator><creator>Todaka, Daisuke</creator><creator>Nakashima, Kazuo</creator><creator>Hirayama, Takashi</creator><creator>Shinozaki, Kazuo</creator><creator>Yamaguchi-Shinozaki, Kazuko</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20111201</creationdate><title>An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis</title><author>Kim, June-Sik ; Mizoi, Junya ; Yoshida, Takuya ; Fujita, Yasunari ; Nakajima, Jun ; Ohori, Teppei ; Todaka, Daisuke ; Nakashima, Kazuo ; Hirayama, Takashi ; Shinozaki, Kazuo ; Yamaguchi-Shinozaki, Kazuko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-1186a03dcb5c94fdb3b9ddc1a749152b691746af9541e5aaf9e5eff4b78a294a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abscisic Acid - pharmacology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Base Sequence</topic><topic>Droughts</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Genes, Plant - genetics</topic><topic>Models, Biological</topic><topic>Molecular Sequence Data</topic><topic>Mutation - genetics</topic><topic>Osmosis - drug effects</topic><topic>Protein Binding - drug effects</topic><topic>Response Elements - genetics</topic><topic>Sequence Deletion - genetics</topic><topic>Stress, Physiological - drug effects</topic><topic>Stress, Physiological - genetics</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcriptional Activation - drug effects</topic><topic>Transcriptional Activation - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, June-Sik</creatorcontrib><creatorcontrib>Mizoi, Junya</creatorcontrib><creatorcontrib>Yoshida, Takuya</creatorcontrib><creatorcontrib>Fujita, Yasunari</creatorcontrib><creatorcontrib>Nakajima, Jun</creatorcontrib><creatorcontrib>Ohori, Teppei</creatorcontrib><creatorcontrib>Todaka, Daisuke</creatorcontrib><creatorcontrib>Nakashima, Kazuo</creatorcontrib><creatorcontrib>Hirayama, Takashi</creatorcontrib><creatorcontrib>Shinozaki, Kazuo</creatorcontrib><creatorcontrib>Yamaguchi-Shinozaki, Kazuko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, June-Sik</au><au>Mizoi, Junya</au><au>Yoshida, Takuya</au><au>Fujita, Yasunari</au><au>Nakajima, Jun</au><au>Ohori, Teppei</au><au>Todaka, Daisuke</au><au>Nakashima, Kazuo</au><au>Hirayama, Takashi</au><au>Shinozaki, Kazuo</au><au>Yamaguchi-Shinozaki, Kazuko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>52</volume><issue>12</issue><spage>2136</spage><epage>2146</epage><pages>2136-2146</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>In plants, osmotic stress-responsive transcriptional regulation depends mainly on two major classes of cis-acting elements found in the promoter regions of stress-inducible genes: ABA-responsive elements (ABREs) and dehydration-responsive elements (DREs). ABRE has been shown to perceive ABA-mediated osmotic stress signals, whereas DRE is known to be involved in an ABA-independent pathway. Previously, we reported that the transcription factor DRE-BINDING PROTEIN 2A (DREB2A) regulates DRE-mediated transcription of target genes under osmotic stress conditions in Arabidopsis (Arabidopsis thaliana). However, the transcriptional regulation of DREB2A itself remains largely uncharacterized. To elucidate the transcriptional mechanism associated with the DREB2A gene under osmotic stress conditions, we generated a series of truncated and base-substituted variants of the DREB2A promoter and evaluated their transcriptional activities individually. We found that both ABRE and coupling element 3 (CE3)-like sequences located approximately −100 bp from the transcriptional initiation site are necessary for the dehydration-responsive expression of DREB2A. Coupling our transient expression analyses with yeast one-hybrid and chromatin immunoprecipitation (ChIP) assays indicated that the ABRE-BINDING PROTEIN 1 (AREB1), AREB2 and ABRE-BINDING FACTOR 3 (ABF3) bZIP transcription factors can bind to and activate the DREB2A promoter in an ABRE-dependent manner. Exogenous ABA application induced only a modest accumulation of the DREB2A transcript when compared with the osmotic stress treatment. However, the osmotic stress-induced DREB2A expression was found to be markedly impaired in several ABA-deficient and ABA-insensitive mutants. These results suggest that in addition to an ABA-independent pathway, the ABA-dependent pathway plays a positive role in the osmotic stress-responsive expression of DREB2A.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>22025559</pmid><doi>10.1093/pcp/pcr143</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abscisic Acid - pharmacology Arabidopsis - genetics Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Base Sequence Droughts Gene Expression Regulation, Plant - drug effects Genes, Plant - genetics Models, Biological Molecular Sequence Data Mutation - genetics Osmosis - drug effects Protein Binding - drug effects Response Elements - genetics Sequence Deletion - genetics Stress, Physiological - drug effects Stress, Physiological - genetics Transcription Factors - genetics Transcription Factors - metabolism Transcriptional Activation - drug effects Transcriptional Activation - genetics |
| title | An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis |
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