Nanocarriers for Cytoplasmic Delivery: Cellular Uptake and Intracellular Fate of Chitosan and Hyaluronic Acid-Coated Chitosan Nanoparticles in a Phagocytic Cell Model
The cellular uptake of hyaluronic‐acid‐coated, negatively charged chitosan/triphosphate nanoparticles and that of uncoated, positively charged ones is investigated by studying cellular localization, uptake kinetics and mechanism of internalization in J774.2 macrophages, using non‐phagocytic L929 fib...
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| Veröffentlicht in: | Macromolecular bioscience 2011-12, Vol.11 (12), p.1747-1760 |
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| creator | Zaki, Noha M. Nasti, Alessandro Tirelli, Nicola |
| description | The cellular uptake of hyaluronic‐acid‐coated, negatively charged chitosan/triphosphate nanoparticles and that of uncoated, positively charged ones is investigated by studying cellular localization, uptake kinetics and mechanism of internalization in J774.2 macrophages, using non‐phagocytic L929 fibroblasts as a control for uncoated nanoparticles. Both kinds of nanoparticles undergo endosomal escape and adopt a similar clathrin‐based endocytic mechanism. The surface decoration with HA profoundly influences the kinetics of cellular uptake, with an at least two orders of magnitude slower kinetics, but also the nature of the binding on the cellular surface.
The endocytosis of chitosan nanoparticles (green) with and without hyaluronic acid‐coating is studied in murine macrophages. Both coated and uncoated nanoparticles evade endolysosomal compartments (red), but the hyaluronic acid coating controls stability in media, cellular interactions and uptake kinetics. |
| doi_str_mv | 10.1002/mabi.201100156 |
| format | Article |
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The endocytosis of chitosan nanoparticles (green) with and without hyaluronic acid‐coating is studied in murine macrophages. Both coated and uncoated nanoparticles evade endolysosomal compartments (red), but the hyaluronic acid coating controls stability in media, cellular interactions and uptake kinetics.</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Cell Line</subject><subject>chitosan</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - metabolism</subject><subject>clathrin</subject><subject>Coated Materials, Biocompatible - chemical synthesis</subject><subject>Coated Materials, Biocompatible - metabolism</subject><subject>Coated Materials, Biocompatible - pharmacology</subject><subject>Drug Carriers - chemical synthesis</subject><subject>Drug Carriers - metabolism</subject><subject>Drug Carriers - pharmacology</subject><subject>endocytosis</subject><subject>Endocytosis - drug effects</subject><subject>Endosomes - metabolism</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>hyaluronic acid</subject><subject>Hyaluronic Acid - chemistry</subject><subject>Hyaluronic Acid - metabolism</subject><subject>Macrophages - cytology</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Microscopy, Fluorescence</subject><subject>Nanoparticles</subject><subject>Particle Size</subject><subject>Static Electricity</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EoqWwZYm8Y5XBThwnZjeEdjpSp-1iCkvrxnGoqRMHOynkhXhOPJ12YIe88M_9zrnyPQi9pWRBCUk_dFCbRUpovNCcP0PHlFOe5FTkzw_nsjhCr0L4HpGiFOlLdJTGOqMFPUa_L6F3Crw32gfcOo-reXSDhdAZhT9ra-61nz_iSls7WfD4ZhjhTmPoG7zuRw_qqXAGo8auxdWtGV2A_gE5n8FO3vXRa6lMk1QuUs1fZtd9AD8aZXXAJorw9S18c2qOTw9N8cY12r5GL1qwQb953E_QzdnptjpPLq5W62p5kaisTHnCGdQsJS3UoKkgpWYkL6DJBDDO44g4ExnkTOlWlTWPS1EGKRDGBMsYJdkJer_3Hbz7Mekwys6E3Reh124KUlAqsjhEHsnFnlTeheB1KwdvOvCzpETuopG7aOQhmih492g91Z1uDvhTFhEQe-CnsXr-j53cLD-t_zVP9loTRv3roAV_J3mRFbn8ermS6Xa72vDtF7nJ_gDSWqwH</recordid><startdate>20111208</startdate><enddate>20111208</enddate><creator>Zaki, Noha M.</creator><creator>Nasti, Alessandro</creator><creator>Tirelli, Nicola</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111208</creationdate><title>Nanocarriers for Cytoplasmic Delivery: Cellular Uptake and Intracellular Fate of Chitosan and Hyaluronic Acid-Coated Chitosan Nanoparticles in a Phagocytic Cell Model</title><author>Zaki, Noha M. ; Nasti, Alessandro ; Tirelli, Nicola</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3826-64ab420fabae1908e4057ad39a4661006493a54cefc8b6b6bc14a2a0449434103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>Cell Line</topic><topic>chitosan</topic><topic>Chitosan - chemistry</topic><topic>Chitosan - metabolism</topic><topic>clathrin</topic><topic>Coated Materials, Biocompatible - chemical synthesis</topic><topic>Coated Materials, Biocompatible - metabolism</topic><topic>Coated Materials, Biocompatible - pharmacology</topic><topic>Drug Carriers - chemical synthesis</topic><topic>Drug Carriers - metabolism</topic><topic>Drug Carriers - pharmacology</topic><topic>endocytosis</topic><topic>Endocytosis - drug effects</topic><topic>Endosomes - metabolism</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - metabolism</topic><topic>hyaluronic acid</topic><topic>Hyaluronic Acid - chemistry</topic><topic>Hyaluronic Acid - metabolism</topic><topic>Macrophages - cytology</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>Microscopy, Fluorescence</topic><topic>Nanoparticles</topic><topic>Particle Size</topic><topic>Static Electricity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zaki, Noha M.</creatorcontrib><creatorcontrib>Nasti, Alessandro</creatorcontrib><creatorcontrib>Tirelli, Nicola</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zaki, Noha M.</au><au>Nasti, Alessandro</au><au>Tirelli, Nicola</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanocarriers for Cytoplasmic Delivery: Cellular Uptake and Intracellular Fate of Chitosan and Hyaluronic Acid-Coated Chitosan Nanoparticles in a Phagocytic Cell Model</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2011-12-08</date><risdate>2011</risdate><volume>11</volume><issue>12</issue><spage>1747</spage><epage>1760</epage><pages>1747-1760</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>The cellular uptake of hyaluronic‐acid‐coated, negatively charged chitosan/triphosphate nanoparticles and that of uncoated, positively charged ones is investigated by studying cellular localization, uptake kinetics and mechanism of internalization in J774.2 macrophages, using non‐phagocytic L929 fibroblasts as a control for uncoated nanoparticles. Both kinds of nanoparticles undergo endosomal escape and adopt a similar clathrin‐based endocytic mechanism. The surface decoration with HA profoundly influences the kinetics of cellular uptake, with an at least two orders of magnitude slower kinetics, but also the nature of the binding on the cellular surface.
The endocytosis of chitosan nanoparticles (green) with and without hyaluronic acid‐coating is studied in murine macrophages. Both coated and uncoated nanoparticles evade endolysosomal compartments (red), but the hyaluronic acid coating controls stability in media, cellular interactions and uptake kinetics.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>21954171</pmid><doi>10.1002/mabi.201100156</doi><tpages>14</tpages></addata></record> |
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| subjects | Animals Binding Sites Cell Line chitosan Chitosan - chemistry Chitosan - metabolism clathrin Coated Materials, Biocompatible - chemical synthesis Coated Materials, Biocompatible - metabolism Coated Materials, Biocompatible - pharmacology Drug Carriers - chemical synthesis Drug Carriers - metabolism Drug Carriers - pharmacology endocytosis Endocytosis - drug effects Endosomes - metabolism Fibroblasts - cytology Fibroblasts - metabolism hyaluronic acid Hyaluronic Acid - chemistry Hyaluronic Acid - metabolism Macrophages - cytology Macrophages - metabolism Mice Microscopy, Fluorescence Nanoparticles Particle Size Static Electricity |
| title | Nanocarriers for Cytoplasmic Delivery: Cellular Uptake and Intracellular Fate of Chitosan and Hyaluronic Acid-Coated Chitosan Nanoparticles in a Phagocytic Cell Model |
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