Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C C double bond migration useful for conjugated fatty acid synthesis
► A novel multi-component enzyme system for linoleate isomerization was identified. ► The linoleate isomerase system of lactic acid bacteria consists of three enzymes. ► The genes encoding elaborate machinery of CLA synthesis was identified. ► A hydration reaction occurred as part of the reaction of...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2011-12, Vol.416 (1), p.188-193 |
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| creator | Kishino, Shigenobu Park, Si-Bum Takeuchi, Michiki Yokozeki, Kenzo Shimizu, Sakayu Ogawa, Jun |
| description | ► A novel multi-component enzyme system for linoleate isomerization was identified. ► The linoleate isomerase system of lactic acid bacteria consists of three enzymes. ► The genes encoding elaborate machinery of CLA synthesis was identified. ► A hydration reaction occurred as part of the reaction of linoleate isomerization.
Linoleic acid isomerase was identified as a multi-component enzyme system that consists of three enzymes that exist in both the membrane and soluble fractions of
Lactobacillus plantarum. One enzyme (CLA-HY) is present in the membrane fraction, while two enzymes (CLA-DH and CLA-DC) exist in the soluble fraction. Three
Escherichia coli transformants expressing CLA-HY, CLA-DH, and CLA-DC were constructed. Conjugated linoleic acid (CLA) and 10-hydroxy-12-octadecenoic acid were generated from linoleic acid only when all these three
E. coli transformants were used as catalysts simultaneously. CLA-HY catalyzed the hydration reaction, a part of linoleic acid isomerization, to produce 10-hydroxy-12-octadecenoic acid. This multi-component enzyme system required oxidoreduction cofactors such as NADH and FAD. This is the first report to reveal enzymes genes and the elaborate machinery that synthesizes CLA, especially an important isomer of
cis-9,
trans-11-CLA, in lactic acid bacteria. |
| doi_str_mv | 10.1016/j.bbrc.2011.11.022 |
| format | Article |
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Linoleic acid isomerase was identified as a multi-component enzyme system that consists of three enzymes that exist in both the membrane and soluble fractions of
Lactobacillus plantarum. One enzyme (CLA-HY) is present in the membrane fraction, while two enzymes (CLA-DH and CLA-DC) exist in the soluble fraction. Three
Escherichia coli transformants expressing CLA-HY, CLA-DH, and CLA-DC were constructed. Conjugated linoleic acid (CLA) and 10-hydroxy-12-octadecenoic acid were generated from linoleic acid only when all these three
E. coli transformants were used as catalysts simultaneously. CLA-HY catalyzed the hydration reaction, a part of linoleic acid isomerization, to produce 10-hydroxy-12-octadecenoic acid. This multi-component enzyme system required oxidoreduction cofactors such as NADH and FAD. This is the first report to reveal enzymes genes and the elaborate machinery that synthesizes CLA, especially an important isomer of
cis-9,
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Linoleic acid isomerase was identified as a multi-component enzyme system that consists of three enzymes that exist in both the membrane and soluble fractions of
Lactobacillus plantarum. One enzyme (CLA-HY) is present in the membrane fraction, while two enzymes (CLA-DH and CLA-DC) exist in the soluble fraction. Three
Escherichia coli transformants expressing CLA-HY, CLA-DH, and CLA-DC were constructed. Conjugated linoleic acid (CLA) and 10-hydroxy-12-octadecenoic acid were generated from linoleic acid only when all these three
E. coli transformants were used as catalysts simultaneously. CLA-HY catalyzed the hydration reaction, a part of linoleic acid isomerization, to produce 10-hydroxy-12-octadecenoic acid. This multi-component enzyme system required oxidoreduction cofactors such as NADH and FAD. This is the first report to reveal enzymes genes and the elaborate machinery that synthesizes CLA, especially an important isomer of
cis-9,
trans-11-CLA, in lactic acid bacteria.</description><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Catalysis</subject><subject>CLA</subject><subject>Cloning, Molecular</subject><subject>Conjugate linoleic acid</subject><subject>Enzymes - chemistry</subject><subject>Enzymes - genetics</subject><subject>Enzymes - isolation & purification</subject><subject>Lactic acid bacteria</subject><subject>Lactobacillus plantarum</subject><subject>Lactobacillus plantarum - enzymology</subject><subject>Lactobacillus plantarum - genetics</subject><subject>Linoleic acid isomerase</subject><subject>Linoleic Acids, Conjugated - biosynthesis</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctqHDEQRUVIiCdOfiCLoF1WPdGjn5BNGJwHGHsTQ3aiWqoea1BLE0ltaH9EvtkaxskyqEAqOHVAdQl5z9mWM95-OmzHMeqtYJxvSzEhXpANZwOrBGf1S7JhjLWVGPivC_ImpQMrYN0Or8mFEGyQvew25M9NeEBH58VlW-kwH4NHnyn6x3VGOoO-tx7jSq2nDnS2moK2ho7ljdEC1ZDBrY_W7-muHBOW0SEdgzd0tvsI2QZPl4TT4ugUItXBH5Y9ZDR0gpzXsy6tPt9jsukteTWBS_ju-b4kd1-vfu6-V9e3337svlxXWjY8V13HWQ9mnKCDvmWNFCh7Dabngo0tmlZMpdESJ9n00pi6llBL3rFetE2DtbwkH8_eYwy_F0xZzTZpdA48hiWpgfNBnryFFGdSx5BSxEkdo50hroozdYpBHdQpBnWKQZUqMZShD8_6ZZzR_Bv5u_cCfD4DWD75YDGqpC16jcZG1FmZYP_nfwKp65t8</recordid><startdate>20111209</startdate><enddate>20111209</enddate><creator>Kishino, Shigenobu</creator><creator>Park, Si-Bum</creator><creator>Takeuchi, Michiki</creator><creator>Yokozeki, Kenzo</creator><creator>Shimizu, Sakayu</creator><creator>Ogawa, Jun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111209</creationdate><title>Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C C double bond migration useful for conjugated fatty acid synthesis</title><author>Kishino, Shigenobu ; Park, Si-Bum ; Takeuchi, Michiki ; Yokozeki, Kenzo ; Shimizu, Sakayu ; Ogawa, Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-77108adbfa7a860532e38cad8120b6ed62fad8c3ef3583dd443a4317082655e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Catalysis</topic><topic>CLA</topic><topic>Cloning, Molecular</topic><topic>Conjugate linoleic acid</topic><topic>Enzymes - chemistry</topic><topic>Enzymes - genetics</topic><topic>Enzymes - isolation & purification</topic><topic>Lactic acid bacteria</topic><topic>Lactobacillus plantarum</topic><topic>Lactobacillus plantarum - enzymology</topic><topic>Lactobacillus plantarum - genetics</topic><topic>Linoleic acid isomerase</topic><topic>Linoleic Acids, Conjugated - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kishino, Shigenobu</creatorcontrib><creatorcontrib>Park, Si-Bum</creatorcontrib><creatorcontrib>Takeuchi, Michiki</creatorcontrib><creatorcontrib>Yokozeki, Kenzo</creatorcontrib><creatorcontrib>Shimizu, Sakayu</creatorcontrib><creatorcontrib>Ogawa, Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kishino, Shigenobu</au><au>Park, Si-Bum</au><au>Takeuchi, Michiki</au><au>Yokozeki, Kenzo</au><au>Shimizu, Sakayu</au><au>Ogawa, Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C C double bond migration useful for conjugated fatty acid synthesis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2011-12-09</date><risdate>2011</risdate><volume>416</volume><issue>1</issue><spage>188</spage><epage>193</epage><pages>188-193</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>► A novel multi-component enzyme system for linoleate isomerization was identified. ► The linoleate isomerase system of lactic acid bacteria consists of three enzymes. ► The genes encoding elaborate machinery of CLA synthesis was identified. ► A hydration reaction occurred as part of the reaction of linoleate isomerization.
Linoleic acid isomerase was identified as a multi-component enzyme system that consists of three enzymes that exist in both the membrane and soluble fractions of
Lactobacillus plantarum. One enzyme (CLA-HY) is present in the membrane fraction, while two enzymes (CLA-DH and CLA-DC) exist in the soluble fraction. Three
Escherichia coli transformants expressing CLA-HY, CLA-DH, and CLA-DC were constructed. Conjugated linoleic acid (CLA) and 10-hydroxy-12-octadecenoic acid were generated from linoleic acid only when all these three
E. coli transformants were used as catalysts simultaneously. CLA-HY catalyzed the hydration reaction, a part of linoleic acid isomerization, to produce 10-hydroxy-12-octadecenoic acid. This multi-component enzyme system required oxidoreduction cofactors such as NADH and FAD. This is the first report to reveal enzymes genes and the elaborate machinery that synthesizes CLA, especially an important isomer of
cis-9,
trans-11-CLA, in lactic acid bacteria.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22093837</pmid><doi>10.1016/j.bbrc.2011.11.022</doi><tpages>6</tpages></addata></record> |
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| subjects | Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Catalysis CLA Cloning, Molecular Conjugate linoleic acid Enzymes - chemistry Enzymes - genetics Enzymes - isolation & purification Lactic acid bacteria Lactobacillus plantarum Lactobacillus plantarum - enzymology Lactobacillus plantarum - genetics Linoleic acid isomerase Linoleic Acids, Conjugated - biosynthesis |
| title | Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C C double bond migration useful for conjugated fatty acid synthesis |
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