Specific IgG and IgA to larvae, parthenogenetic females, and eggs of Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis
Abstract The aim of this study was to detect levels of IgG and IgA by enzyme-linked immunosorbent assay (ELISA) using alkaline extracts of larvae, adult female worms, and eggs of Strongyloides venezuelensis as antigen. One hundred twenty serum samples divided into 3 groups were analysed: group I (40...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2012, Vol.72 (1), p.79-84 |
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| creator | Gonçalves, Ana Lúcia Ribeiro Rocha, Camila Alves Gonzaga, Henrique Tomaz Gonçalves-Pires, Maria do Rosário de Fátima Ueta, Marlene Tiduko Costa-Cruz, Julia Maria |
| description | Abstract The aim of this study was to detect levels of IgG and IgA by enzyme-linked immunosorbent assay (ELISA) using alkaline extracts of larvae, adult female worms, and eggs of Strongyloides venezuelensis as antigen. One hundred twenty serum samples divided into 3 groups were analysed: group I (40 strongyloidiasis patients), group II (40 patients with other parasitic infections), and group III (40 healthy subjects). Statistical variations were analyzed using analysis of variance. There was a significant statistical difference ( P < 0.001) in the detection of antibodies in group I between larvae and female antigens and between larvae and egg antigens, with higher positivity using larvae antigen. The larvae antigen showed the highest values for sensitivity, specificity, and diagnostic efficiency in ELISA. This study is the first that examines the use of adult female worm and egg antigens to detect antibodies for human strongyloidiasis diagnosis compared with the larval extract. By comparing all 3 extracts, larval antigens demonstrated better diagnostic parameters. |
| doi_str_mv | 10.1016/j.diagmicrobio.2011.09.011 |
| format | Article |
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One hundred twenty serum samples divided into 3 groups were analysed: group I (40 strongyloidiasis patients), group II (40 patients with other parasitic infections), and group III (40 healthy subjects). Statistical variations were analyzed using analysis of variance. There was a significant statistical difference ( P < 0.001) in the detection of antibodies in group I between larvae and female antigens and between larvae and egg antigens, with higher positivity using larvae antigen. The larvae antigen showed the highest values for sensitivity, specificity, and diagnostic efficiency in ELISA. This study is the first that examines the use of adult female worm and egg antigens to detect antibodies for human strongyloidiasis diagnosis compared with the larval extract. 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Psychology ; Helminthic diseases ; Humans ; Immunoglobulin A - blood ; Immunoglobulin G - blood ; Immunoglobulins ; Immunologic Tests - methods ; Infectious Disease ; Infectious diseases ; Internal Medicine ; Larva - immunology ; Medical sciences ; Microbiology ; Parasitic diseases ; Parasitology - methods ; Rats ; Sensitivity and Specificity ; Strongyloides ; Strongyloides - immunology ; Strongyloidiasis ; Strongyloidiasis - diagnosis ; Strongyloidosis</subject><ispartof>Diagnostic microbiology and infectious disease, 2012, Vol.72 (1), p.79-84</ispartof><rights>Elsevier Inc.</rights><rights>2012 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Inc. 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One hundred twenty serum samples divided into 3 groups were analysed: group I (40 strongyloidiasis patients), group II (40 patients with other parasitic infections), and group III (40 healthy subjects). Statistical variations were analyzed using analysis of variance. There was a significant statistical difference ( P < 0.001) in the detection of antibodies in group I between larvae and female antigens and between larvae and egg antigens, with higher positivity using larvae antigen. The larvae antigen showed the highest values for sensitivity, specificity, and diagnostic efficiency in ELISA. This study is the first that examines the use of adult female worm and egg antigens to detect antibodies for human strongyloidiasis diagnosis compared with the larval extract. 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Psychology</subject><subject>Helminthic diseases</subject><subject>Humans</subject><subject>Immunoglobulin A - blood</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulins</subject><subject>Immunologic Tests - methods</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>Larva - immunology</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Parasitic diseases</subject><subject>Parasitology - methods</subject><subject>Rats</subject><subject>Sensitivity and Specificity</subject><subject>Strongyloides</subject><subject>Strongyloides - immunology</subject><subject>Strongyloidiasis</subject><subject>Strongyloidiasis - diagnosis</subject><subject>Strongyloidosis</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNklGL1DAQx4t4eOvpV5AgiD5ca5KmbeqDcJx6d3Dgw-pzSNNpL2ubrEm7sH4Lv7FTd_XEBxFCBia__8ww_yTJc0YzRln5epO1VvejNcE31mecMpbROsPwIFkxWdUppRV9mKxolfNUyjo_TR7HuKGU8VrQR8kpR02Rc7FKvq-3YGxnDbnpr4h2LcYLMnky6LDTcE62Okx34HwPDibEOhj1APH8Jwt9H4nvyHoK3vX7wdsWItkh-m2GAVy0kVhHsACx4zg7vwzu_JJG1d08akfivdZqfHmSnHR6iPD0GM-Szx_ef7q8Tm8_Xt1cXtymphD1lHZSF4IXwnRV0XaN4ZVgmnNTVKJp8e4gl1LnsqhaTquyLCEXVBg8DROYzs-Sl4e62-C_zhAnNdpoYBi0Az9HVTNWcylLgeSrf5KMUilFVdUU0TcHFL2JMUCntsGOOuwRUot5aqP-NE8t5ilaKwwofnbsMzcjtL-lv9xC4MUR0NHooQvaGRvvuaJkRSlL5N4dOMD97SwEFY0FZ6C1AcykWm__b563f5Uxg3UWO3-BPcSNn4NDhxRTkSuq1st3W34bw4XkZSHzHwNt1Vw</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Gonçalves, Ana Lúcia Ribeiro</creator><creator>Rocha, Camila Alves</creator><creator>Gonzaga, Henrique Tomaz</creator><creator>Gonçalves-Pires, Maria do Rosário de Fátima</creator><creator>Ueta, Marlene Tiduko</creator><creator>Costa-Cruz, Julia Maria</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>2012</creationdate><title>Specific IgG and IgA to larvae, parthenogenetic females, and eggs of Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis</title><author>Gonçalves, Ana Lúcia Ribeiro ; Rocha, Camila Alves ; Gonzaga, Henrique Tomaz ; Gonçalves-Pires, Maria do Rosário de Fátima ; Ueta, Marlene Tiduko ; Costa-Cruz, Julia Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-f8a54254cf75dfbc2741a22c574bdc57fe388a3857d207666e3404c04cb143853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antigen</topic><topic>Antigens, Helminth</topic><topic>Biological and medical sciences</topic><topic>Diagnosis</topic><topic>Diseases caused by nematodes</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Helminthic diseases</topic><topic>Humans</topic><topic>Immunoglobulin A - blood</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulins</topic><topic>Immunologic Tests - methods</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Internal Medicine</topic><topic>Larva - immunology</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Parasitic diseases</topic><topic>Parasitology - methods</topic><topic>Rats</topic><topic>Sensitivity and Specificity</topic><topic>Strongyloides</topic><topic>Strongyloides - immunology</topic><topic>Strongyloidiasis</topic><topic>Strongyloidiasis - diagnosis</topic><topic>Strongyloidosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonçalves, Ana Lúcia Ribeiro</creatorcontrib><creatorcontrib>Rocha, Camila Alves</creatorcontrib><creatorcontrib>Gonzaga, Henrique Tomaz</creatorcontrib><creatorcontrib>Gonçalves-Pires, Maria do Rosário de Fátima</creatorcontrib><creatorcontrib>Ueta, Marlene Tiduko</creatorcontrib><creatorcontrib>Costa-Cruz, Julia Maria</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonçalves, Ana Lúcia Ribeiro</au><au>Rocha, Camila Alves</au><au>Gonzaga, Henrique Tomaz</au><au>Gonçalves-Pires, Maria do Rosário de Fátima</au><au>Ueta, Marlene Tiduko</au><au>Costa-Cruz, Julia Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific IgG and IgA to larvae, parthenogenetic females, and eggs of Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2012</date><risdate>2012</risdate><volume>72</volume><issue>1</issue><spage>79</spage><epage>84</epage><pages>79-84</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Abstract The aim of this study was to detect levels of IgG and IgA by enzyme-linked immunosorbent assay (ELISA) using alkaline extracts of larvae, adult female worms, and eggs of Strongyloides venezuelensis as antigen. 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By comparing all 3 extracts, larval antigens demonstrated better diagnostic parameters.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>22015324</pmid><doi>10.1016/j.diagmicrobio.2011.09.011</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 2012, Vol.72 (1), p.79-84 |
| issn | 0732-8893 1879-0070 |
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| subjects | Animals Antibodies, Helminth - blood Antigen Antigens, Helminth Biological and medical sciences Diagnosis Diseases caused by nematodes Enzyme-Linked Immunosorbent Assay - methods Female Fundamental and applied biological sciences. Psychology Helminthic diseases Humans Immunoglobulin A - blood Immunoglobulin G - blood Immunoglobulins Immunologic Tests - methods Infectious Disease Infectious diseases Internal Medicine Larva - immunology Medical sciences Microbiology Parasitic diseases Parasitology - methods Rats Sensitivity and Specificity Strongyloides Strongyloides - immunology Strongyloidiasis Strongyloidiasis - diagnosis Strongyloidosis |
| title | Specific IgG and IgA to larvae, parthenogenetic females, and eggs of Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis |
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