Experimental improvements in combining CARD-FISH and flow cytometry for bacterial cell quantification

Flow cytometry and Fluorescence In Situ Hybridization are common methods of identifying and quantifying bacterial cells. The combination of cytometric rapidity and multi-parametric accuracy with the phylogenetic specificity of oligonucleotide FISH probes has been regarded as a powerful and emerging...

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Veröffentlicht in:	Journal of microbiological methods 2011-12, Vol.87 (3), p.309-315
Hauptverfasser:	Manti, Anita, Boi, Paola, Amalfitano, Stefano, Puddu, Alberto, Papa, Stefano
Format:	Artikel
Sprache:	eng
Schlagworte:	bacteria Bacterial Load - methods Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences CARD-FISH Cell detachment procedure E. coli Escherichia coli Escherichia coli - isolation & purification filters Flow Cytometry Flow Cytometry - methods fluorescence fluorescence in situ hybridization Fundamental and applied biological sciences. Psychology In Situ Hybridization, Fluorescence - methods Marine Marine bacteria microbial communities Microbiology oligonucleotides phylogeny Water Microbiology
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