Effects of dithioerythritol on ram semen after the freeze–thawing process
The aim of this study was to evaluate the effects of dithioerythritol added to cryopreservation extender on the post-thawed sperm parameters, lipid peroxidation and antioxidant activities of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen...
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| Veröffentlicht in: | Cryobiology 2011-12, Vol.63 (3), p.152-156 |
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| creator | Başpınar, Nuri Çoyan, Kenan Bucak, Mustafa Numan Tuncer, Pürhan Barbaros |
| description | The aim of this study was to evaluate the effects of dithioerythritol added to cryopreservation extender on the post-thawed sperm parameters, lipid peroxidation and antioxidant activities of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing 0.5, 1, and 2mM dithiothreitol and no antioxidant (control), were cooled to 5°C and frozen in 0.25ml French straws. Frozen straws were then thawed individually at 37°C for 20s in a water bath for evaluation.
The addition of dithioerythritol at 0.5 and 2mM doses led to higher percentages of subjective motility (62.9±4.2% and 63.6±1.8%) compared to control (52.0±4.9%, P |
| doi_str_mv | 10.1016/j.cryobiol.2011.08.004 |
| format | Article |
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The addition of dithioerythritol at 0.5 and 2mM doses led to higher percentages of subjective motility (62.9±4.2% and 63.6±1.8%) compared to control (52.0±4.9%, P<0.05). As regards CASA motility, dithioerythritol 0.25 and 2mM (60.2±4.5% and 59.6±1.2%) groups were higher from that of control (44.2±8.7%, P<0.05). For the CASA progressive motility, 0.25, 0.5 and 2mM doses of dithioerythritol (22.0±2.1%, 21.7±2.5% and 24.0±1.2%) had increasing effect in comparison to control (15.0±2.5%). Dithioerythritol at 1 and 2mM doses for ALH provided higher values compared to the control (P<0.001) following the freeze–thawing process. Supplementation with dithiothreitol did not significantly affect the integrities of sperm membrane and acrosome, and mitochondrial activities. No significant differences were observed in biochemical parameters among the groups (P>0.05). Findings of this study showed that dithioerythritol supplementation in semen extenders, was of greater benefit to sperm motility of frozen–thawed ram sperm.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/j.cryobiol.2011.08.004</identifier><identifier>PMID: 21945818</identifier><identifier>CODEN: CRYBAS</identifier><language>eng</language><publisher>Kidlington: Elsevier Inc</publisher><subject>Acrosome - drug effects ; Acrosome - metabolism ; Animals ; Antioxidants - pharmacology ; Biological and medical sciences ; Catalase - metabolism ; Cell Survival - drug effects ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; Dithioerythritol ; Dithioerythritol - pharmacology ; Freezing–thawing ; Fundamental and applied biological sciences. Psychology ; Glutathione Peroxidase - metabolism ; Lipid Peroxidation - drug effects ; Male ; Mammalian reproduction. General aspects ; Microscopy, Fluorescence ; Ram sperm ; Semen - drug effects ; Semen - physiology ; Semen Analysis ; Semen Preservation - methods ; Sheep ; Sperm motility ; Sperm Motility - drug effects ; Vertebrates: reproduction</subject><ispartof>Cryobiology, 2011-12, Vol.63 (3), p.152-156</ispartof><rights>2011 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-98aab659721acf01fd3b7fd67a28cb1552cd1483dcd8030f515c2610231028da3</citedby><cites>FETCH-LOGICAL-c429t-98aab659721acf01fd3b7fd67a28cb1552cd1483dcd8030f515c2610231028da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0011224011001234$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25334920$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21945818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Başpınar, Nuri</creatorcontrib><creatorcontrib>Çoyan, Kenan</creatorcontrib><creatorcontrib>Bucak, Mustafa Numan</creatorcontrib><creatorcontrib>Tuncer, Pürhan Barbaros</creatorcontrib><title>Effects of dithioerythritol on ram semen after the freeze–thawing process</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>The aim of this study was to evaluate the effects of dithioerythritol added to cryopreservation extender on the post-thawed sperm parameters, lipid peroxidation and antioxidant activities of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing 0.5, 1, and 2mM dithiothreitol and no antioxidant (control), were cooled to 5°C and frozen in 0.25ml French straws. Frozen straws were then thawed individually at 37°C for 20s in a water bath for evaluation.
The addition of dithioerythritol at 0.5 and 2mM doses led to higher percentages of subjective motility (62.9±4.2% and 63.6±1.8%) compared to control (52.0±4.9%, P<0.05). As regards CASA motility, dithioerythritol 0.25 and 2mM (60.2±4.5% and 59.6±1.2%) groups were higher from that of control (44.2±8.7%, P<0.05). For the CASA progressive motility, 0.25, 0.5 and 2mM doses of dithioerythritol (22.0±2.1%, 21.7±2.5% and 24.0±1.2%) had increasing effect in comparison to control (15.0±2.5%). Dithioerythritol at 1 and 2mM doses for ALH provided higher values compared to the control (P<0.001) following the freeze–thawing process. Supplementation with dithiothreitol did not significantly affect the integrities of sperm membrane and acrosome, and mitochondrial activities. No significant differences were observed in biochemical parameters among the groups (P>0.05). Findings of this study showed that dithioerythritol supplementation in semen extenders, was of greater benefit to sperm motility of frozen–thawed ram sperm.</description><subject>Acrosome - drug effects</subject><subject>Acrosome - metabolism</subject><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Catalase - metabolism</subject><subject>Cell Survival - drug effects</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Dithioerythritol</subject><subject>Dithioerythritol - pharmacology</subject><subject>Freezing–thawing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Male</subject><subject>Mammalian reproduction. General aspects</subject><subject>Microscopy, Fluorescence</subject><subject>Ram sperm</subject><subject>Semen - drug effects</subject><subject>Semen - physiology</subject><subject>Semen Analysis</subject><subject>Semen Preservation - methods</subject><subject>Sheep</subject><subject>Sperm motility</subject><subject>Sperm Motility - drug effects</subject><subject>Vertebrates: reproduction</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAQgC0EokvhFSpfEKeE8U8S-waqyo-oxAXOlmOPWa-SuNhZquXEO_CGPAmudgvHHkZzmG9-9A0hFwxaBqx_vWtdPqQxpqnlwFgLqgWQj8iGgYaGC80fkw3USsO5hDPyrJQdAPSDkE_JGWdadoqpDfl0FQK6tdAUqI_rNibMh3Wb45ommhaa7UwLzrhQG1bMdN0iDRnxJ_759Xvd2tu4fKM3OTks5Tl5EuxU8MUpn5Ov766-XH5orj-__3j59rpxkuu10crase_0wJl1AVjwYhyC7wfLlRtZ13HnmVTCO69AQOhY53jPgIsayltxTl4d59a93_dYVjPH4nCa7IJpX4xmjPWy18PDJPRdp5TUleyPpMuplIzB3OQ423wwDMydcbMz98bNnXEDylTjtfHitGI_zuj_td0rrsDLE2CLs1PIdnGx_Oc6IaTmULk3Rw6ruh8Rsyku4uLQx1w_ZHyKD93yF_4zoxM</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Başpınar, Nuri</creator><creator>Çoyan, Kenan</creator><creator>Bucak, Mustafa Numan</creator><creator>Tuncer, Pürhan Barbaros</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20111201</creationdate><title>Effects of dithioerythritol on ram semen after the freeze–thawing process</title><author>Başpınar, Nuri ; Çoyan, Kenan ; Bucak, Mustafa Numan ; Tuncer, Pürhan Barbaros</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-98aab659721acf01fd3b7fd67a28cb1552cd1483dcd8030f515c2610231028da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acrosome - drug effects</topic><topic>Acrosome - metabolism</topic><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Catalase - metabolism</topic><topic>Cell Survival - drug effects</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Dithioerythritol</topic><topic>Dithioerythritol - pharmacology</topic><topic>Freezing–thawing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Male</topic><topic>Mammalian reproduction. General aspects</topic><topic>Microscopy, Fluorescence</topic><topic>Ram sperm</topic><topic>Semen - drug effects</topic><topic>Semen - physiology</topic><topic>Semen Analysis</topic><topic>Semen Preservation - methods</topic><topic>Sheep</topic><topic>Sperm motility</topic><topic>Sperm Motility - drug effects</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Başpınar, Nuri</creatorcontrib><creatorcontrib>Çoyan, Kenan</creatorcontrib><creatorcontrib>Bucak, Mustafa Numan</creatorcontrib><creatorcontrib>Tuncer, Pürhan Barbaros</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Başpınar, Nuri</au><au>Çoyan, Kenan</au><au>Bucak, Mustafa Numan</au><au>Tuncer, Pürhan Barbaros</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of dithioerythritol on ram semen after the freeze–thawing process</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>63</volume><issue>3</issue><spage>152</spage><epage>156</epage><pages>152-156</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><coden>CRYBAS</coden><abstract>The aim of this study was to evaluate the effects of dithioerythritol added to cryopreservation extender on the post-thawed sperm parameters, lipid peroxidation and antioxidant activities of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing 0.5, 1, and 2mM dithiothreitol and no antioxidant (control), were cooled to 5°C and frozen in 0.25ml French straws. Frozen straws were then thawed individually at 37°C for 20s in a water bath for evaluation.
The addition of dithioerythritol at 0.5 and 2mM doses led to higher percentages of subjective motility (62.9±4.2% and 63.6±1.8%) compared to control (52.0±4.9%, P<0.05). As regards CASA motility, dithioerythritol 0.25 and 2mM (60.2±4.5% and 59.6±1.2%) groups were higher from that of control (44.2±8.7%, P<0.05). For the CASA progressive motility, 0.25, 0.5 and 2mM doses of dithioerythritol (22.0±2.1%, 21.7±2.5% and 24.0±1.2%) had increasing effect in comparison to control (15.0±2.5%). Dithioerythritol at 1 and 2mM doses for ALH provided higher values compared to the control (P<0.001) following the freeze–thawing process. Supplementation with dithiothreitol did not significantly affect the integrities of sperm membrane and acrosome, and mitochondrial activities. No significant differences were observed in biochemical parameters among the groups (P>0.05). Findings of this study showed that dithioerythritol supplementation in semen extenders, was of greater benefit to sperm motility of frozen–thawed ram sperm.</abstract><cop>Kidlington</cop><pub>Elsevier Inc</pub><pmid>21945818</pmid><doi>10.1016/j.cryobiol.2011.08.004</doi><tpages>5</tpages></addata></record> |
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| subjects | Acrosome - drug effects Acrosome - metabolism Animals Antioxidants - pharmacology Biological and medical sciences Catalase - metabolism Cell Survival - drug effects Cryopreservation - methods Cryoprotective Agents - pharmacology Dithioerythritol Dithioerythritol - pharmacology Freezing–thawing Fundamental and applied biological sciences. Psychology Glutathione Peroxidase - metabolism Lipid Peroxidation - drug effects Male Mammalian reproduction. General aspects Microscopy, Fluorescence Ram sperm Semen - drug effects Semen - physiology Semen Analysis Semen Preservation - methods Sheep Sperm motility Sperm Motility - drug effects Vertebrates: reproduction |
| title | Effects of dithioerythritol on ram semen after the freeze–thawing process |
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