Difference in virulence between fluconazole-susceptible and fluconazole-resistant Candida albicans in a mouse model
Summary Two Candida albicans isolates were collected from a HIV‐positive patient with recurrent oropharyngeal candidosis (OPC). One isolate was taken during the first episode of oral candidosis [fluconazole susceptible (FLU‐S), minimal inhibitory concentration (MIC) = 0.25 mg l−1] and the second aft...
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Veröffentlicht in: | Mycoses 2011-09, Vol.54 (5), p.e522-e530 |
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description | Summary
Two Candida albicans isolates were collected from a HIV‐positive patient with recurrent oropharyngeal candidosis (OPC). One isolate was taken during the first episode of oral candidosis [fluconazole susceptible (FLU‐S), minimal inhibitory concentration (MIC) = 0.25 mg l−1] and the second after the patient developed refractory OPC and resistance to fluconazole (FLU‐R, MIC = 64 mg l−1). Both isolates were clonally identical. Different in vitro studies were carried out to assess putative virulence factors of both isolates. Gene expressions of efflux pumps and CSH1 were determined as well as adherence to human epithelial cells, determination of proteinase secretion and biofilm formation activity. Virulence was studied using a disseminated mouse model. All mice challenged with the FLU‐S isolate survived the experiment when FLU was given. However, when FLU was absent, the mortality of the FLU‐S isolate was higher than that of the FLU‐R isolate with no mice surviving the experiment. In vitro studies showed pronounced growth rates of the FLU‐S isolate and a more intense biofilm‐building activity compared with the FLU‐R isolate. The FLU‐R isolate highly up‐regulated MDR1 and CSH1. This isolate also adhered stronger to the epithelial cell line. The results showed that FLU‐S and FLU‐R isolates exhibit different virulence factors, which enable the survival of both isolates in adapted environments. |
doi_str_mv | 10.1111/j.1439-0507.2010.01970.x |
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Two Candida albicans isolates were collected from a HIV‐positive patient with recurrent oropharyngeal candidosis (OPC). One isolate was taken during the first episode of oral candidosis [fluconazole susceptible (FLU‐S), minimal inhibitory concentration (MIC) = 0.25 mg l−1] and the second after the patient developed refractory OPC and resistance to fluconazole (FLU‐R, MIC = 64 mg l−1). Both isolates were clonally identical. Different in vitro studies were carried out to assess putative virulence factors of both isolates. Gene expressions of efflux pumps and CSH1 were determined as well as adherence to human epithelial cells, determination of proteinase secretion and biofilm formation activity. Virulence was studied using a disseminated mouse model. All mice challenged with the FLU‐S isolate survived the experiment when FLU was given. However, when FLU was absent, the mortality of the FLU‐S isolate was higher than that of the FLU‐R isolate with no mice surviving the experiment. In vitro studies showed pronounced growth rates of the FLU‐S isolate and a more intense biofilm‐building activity compared with the FLU‐R isolate. The FLU‐R isolate highly up‐regulated MDR1 and CSH1. This isolate also adhered stronger to the epithelial cell line. The results showed that FLU‐S and FLU‐R isolates exhibit different virulence factors, which enable the survival of both isolates in adapted environments.</description><identifier>ISSN: 0933-7407</identifier><identifier>EISSN: 1439-0507</identifier><identifier>DOI: 10.1111/j.1439-0507.2010.01970.x</identifier><identifier>PMID: 21605180</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>adherence ; Animal models ; Animals ; Antifungal Agents - pharmacology ; biofilm ; Biofilms ; Candida ; Candida albicans ; Candida albicans - classification ; Candida albicans - drug effects ; Candida albicans - isolation & purification ; Candida albicans - pathogenicity ; Candidiasis - microbiology ; Candidiasis - pathology ; Cell Adhesion ; Cell Line ; Disease Models, Animal ; DNA, Fungal - genetics ; Drug Resistance, Fungal ; Epithelial cells ; Epithelial Cells - microbiology ; fluconazole ; Fluconazole - pharmacology ; Gene expression ; Genotype ; Growth rate ; HIV Infections - complications ; Human immunodeficiency virus ; Humans ; Male ; MDR1 protein ; Mice ; Mortality ; Mycological Typing Techniques ; Proteinase ; resistance ; Rodent Diseases - microbiology ; Rodent Diseases - pathology ; Secretion ; Survival Analysis ; Virulence ; virulence factors</subject><ispartof>Mycoses, 2011-09, Vol.54 (5), p.e522-e530</ispartof><rights>2011 Blackwell Verlag GmbH</rights><rights>2011 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4380-bc6fe35d698bb9be9f3a6c14ffe8b4197490e77002a80c2a6a558c0e7eb286fc3</citedby><cites>FETCH-LOGICAL-c4380-bc6fe35d698bb9be9f3a6c14ffe8b4197490e77002a80c2a6a558c0e7eb286fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0507.2010.01970.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0507.2010.01970.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21605180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schulz, Bettina</creatorcontrib><creatorcontrib>Weber, Kai</creatorcontrib><creatorcontrib>Schmidt, Axel</creatorcontrib><creatorcontrib>Borg-von Zepelin, Margarete</creatorcontrib><creatorcontrib>Ruhnke, Markus</creatorcontrib><title>Difference in virulence between fluconazole-susceptible and fluconazole-resistant Candida albicans in a mouse model</title><title>Mycoses</title><addtitle>Mycoses</addtitle><description>Summary
Two Candida albicans isolates were collected from a HIV‐positive patient with recurrent oropharyngeal candidosis (OPC). One isolate was taken during the first episode of oral candidosis [fluconazole susceptible (FLU‐S), minimal inhibitory concentration (MIC) = 0.25 mg l−1] and the second after the patient developed refractory OPC and resistance to fluconazole (FLU‐R, MIC = 64 mg l−1). Both isolates were clonally identical. Different in vitro studies were carried out to assess putative virulence factors of both isolates. Gene expressions of efflux pumps and CSH1 were determined as well as adherence to human epithelial cells, determination of proteinase secretion and biofilm formation activity. Virulence was studied using a disseminated mouse model. All mice challenged with the FLU‐S isolate survived the experiment when FLU was given. However, when FLU was absent, the mortality of the FLU‐S isolate was higher than that of the FLU‐R isolate with no mice surviving the experiment. In vitro studies showed pronounced growth rates of the FLU‐S isolate and a more intense biofilm‐building activity compared with the FLU‐R isolate. The FLU‐R isolate highly up‐regulated MDR1 and CSH1. This isolate also adhered stronger to the epithelial cell line. The results showed that FLU‐S and FLU‐R isolates exhibit different virulence factors, which enable the survival of both isolates in adapted environments.</description><subject>adherence</subject><subject>Animal models</subject><subject>Animals</subject><subject>Antifungal Agents - pharmacology</subject><subject>biofilm</subject><subject>Biofilms</subject><subject>Candida</subject><subject>Candida albicans</subject><subject>Candida albicans - classification</subject><subject>Candida albicans - drug effects</subject><subject>Candida albicans - isolation & purification</subject><subject>Candida albicans - pathogenicity</subject><subject>Candidiasis - microbiology</subject><subject>Candidiasis - pathology</subject><subject>Cell Adhesion</subject><subject>Cell Line</subject><subject>Disease Models, Animal</subject><subject>DNA, Fungal - genetics</subject><subject>Drug Resistance, Fungal</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - microbiology</subject><subject>fluconazole</subject><subject>Fluconazole - pharmacology</subject><subject>Gene expression</subject><subject>Genotype</subject><subject>Growth rate</subject><subject>HIV Infections - complications</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Male</subject><subject>MDR1 protein</subject><subject>Mice</subject><subject>Mortality</subject><subject>Mycological Typing Techniques</subject><subject>Proteinase</subject><subject>resistance</subject><subject>Rodent Diseases - microbiology</subject><subject>Rodent Diseases - pathology</subject><subject>Secretion</subject><subject>Survival Analysis</subject><subject>Virulence</subject><subject>virulence factors</subject><issn>0933-7407</issn><issn>1439-0507</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EotvCX0C5ccoyjh1_HDigtBSkAhIC8XGxbGciefEmS5zQLb--TresxAl88MfM-449fggpKKxpHi82a8qZLqEGua4gR4FqCev9A7I6Jh6SFWjGSslBnpDTlDYAVOpKPCYnFRVQUwUrks5D1-GIvcci9MWvMM7x7uBwukbsiy7Ofujt7yFimebkcTcFF7GwfftXbsQU0mT7qWhyKrS2sNEFb_u01LXFdpgT5rnF-IQ86mxM-PR-PSOfX198at6UVx8u3zavrkrPmYLSedEhq1uhlXPaoe6YFZ7y_F7leG6Ya0ApASqrwFdW2LpWPofQVUp0np2R54e6u3H4OWOazDbkBmK0PebXGJ2_UlBR838qldK8rjinWakOSj8OKY3Ymd0Ytna8MRTMwsZszILALAjMwsbcsTH7bH12f8nsttgejX9gZMHLg-A6RLz578Lm3bdm2WV_efBnELg_-u34wwjJZG2-vL80qvlefWRfwZyzWzZrrjo</recordid><startdate>201109</startdate><enddate>201109</enddate><creator>Schulz, Bettina</creator><creator>Weber, Kai</creator><creator>Schmidt, Axel</creator><creator>Borg-von Zepelin, Margarete</creator><creator>Ruhnke, Markus</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>201109</creationdate><title>Difference in virulence between fluconazole-susceptible and fluconazole-resistant Candida albicans in a mouse model</title><author>Schulz, Bettina ; Weber, Kai ; Schmidt, Axel ; Borg-von Zepelin, Margarete ; Ruhnke, Markus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4380-bc6fe35d698bb9be9f3a6c14ffe8b4197490e77002a80c2a6a558c0e7eb286fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>adherence</topic><topic>Animal models</topic><topic>Animals</topic><topic>Antifungal Agents - pharmacology</topic><topic>biofilm</topic><topic>Biofilms</topic><topic>Candida</topic><topic>Candida albicans</topic><topic>Candida albicans - classification</topic><topic>Candida albicans - drug effects</topic><topic>Candida albicans - isolation & purification</topic><topic>Candida albicans - pathogenicity</topic><topic>Candidiasis - microbiology</topic><topic>Candidiasis - pathology</topic><topic>Cell Adhesion</topic><topic>Cell Line</topic><topic>Disease Models, Animal</topic><topic>DNA, Fungal - genetics</topic><topic>Drug Resistance, Fungal</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - microbiology</topic><topic>fluconazole</topic><topic>Fluconazole - pharmacology</topic><topic>Gene expression</topic><topic>Genotype</topic><topic>Growth rate</topic><topic>HIV Infections - complications</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Male</topic><topic>MDR1 protein</topic><topic>Mice</topic><topic>Mortality</topic><topic>Mycological Typing Techniques</topic><topic>Proteinase</topic><topic>resistance</topic><topic>Rodent Diseases - microbiology</topic><topic>Rodent Diseases - pathology</topic><topic>Secretion</topic><topic>Survival Analysis</topic><topic>Virulence</topic><topic>virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schulz, Bettina</creatorcontrib><creatorcontrib>Weber, Kai</creatorcontrib><creatorcontrib>Schmidt, Axel</creatorcontrib><creatorcontrib>Borg-von Zepelin, Margarete</creatorcontrib><creatorcontrib>Ruhnke, Markus</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Mycoses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schulz, Bettina</au><au>Weber, Kai</au><au>Schmidt, Axel</au><au>Borg-von Zepelin, Margarete</au><au>Ruhnke, Markus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Difference in virulence between fluconazole-susceptible and fluconazole-resistant Candida albicans in a mouse model</atitle><jtitle>Mycoses</jtitle><addtitle>Mycoses</addtitle><date>2011-09</date><risdate>2011</risdate><volume>54</volume><issue>5</issue><spage>e522</spage><epage>e530</epage><pages>e522-e530</pages><issn>0933-7407</issn><eissn>1439-0507</eissn><abstract>Summary
Two Candida albicans isolates were collected from a HIV‐positive patient with recurrent oropharyngeal candidosis (OPC). One isolate was taken during the first episode of oral candidosis [fluconazole susceptible (FLU‐S), minimal inhibitory concentration (MIC) = 0.25 mg l−1] and the second after the patient developed refractory OPC and resistance to fluconazole (FLU‐R, MIC = 64 mg l−1). Both isolates were clonally identical. Different in vitro studies were carried out to assess putative virulence factors of both isolates. Gene expressions of efflux pumps and CSH1 were determined as well as adherence to human epithelial cells, determination of proteinase secretion and biofilm formation activity. Virulence was studied using a disseminated mouse model. All mice challenged with the FLU‐S isolate survived the experiment when FLU was given. However, when FLU was absent, the mortality of the FLU‐S isolate was higher than that of the FLU‐R isolate with no mice surviving the experiment. In vitro studies showed pronounced growth rates of the FLU‐S isolate and a more intense biofilm‐building activity compared with the FLU‐R isolate. The FLU‐R isolate highly up‐regulated MDR1 and CSH1. This isolate also adhered stronger to the epithelial cell line. The results showed that FLU‐S and FLU‐R isolates exhibit different virulence factors, which enable the survival of both isolates in adapted environments.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21605180</pmid><doi>10.1111/j.1439-0507.2010.01970.x</doi><tpages>9</tpages></addata></record> |
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subjects | adherence Animal models Animals Antifungal Agents - pharmacology biofilm Biofilms Candida Candida albicans Candida albicans - classification Candida albicans - drug effects Candida albicans - isolation & purification Candida albicans - pathogenicity Candidiasis - microbiology Candidiasis - pathology Cell Adhesion Cell Line Disease Models, Animal DNA, Fungal - genetics Drug Resistance, Fungal Epithelial cells Epithelial Cells - microbiology fluconazole Fluconazole - pharmacology Gene expression Genotype Growth rate HIV Infections - complications Human immunodeficiency virus Humans Male MDR1 protein Mice Mortality Mycological Typing Techniques Proteinase resistance Rodent Diseases - microbiology Rodent Diseases - pathology Secretion Survival Analysis Virulence virulence factors |
title | Difference in virulence between fluconazole-susceptible and fluconazole-resistant Candida albicans in a mouse model |
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