Electrochemical synthesis of gold nanostructure modified electrode and its development in electrochemical DNA biosensor
In this article, gold nanostructure modified electrodes were achieved by a simple one-step electrodeposition method. The morphologies of modified electrodes could be easily controlled by changing the pH of HAuCl 4 solution. The novel nanoflower-like particles with the nanoplates as the building bloc...
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| Veröffentlicht in: | Biosensors & bioelectronics 2011-12, Vol.30 (1), p.151-157 |
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| creator | Wang, Li Chen, Xiaohong Wang, Xiaoli Han, Xiaoping Liu, Shufeng Zhao, Changzhi |
| description | In this article, gold nanostructure modified electrodes were achieved by a simple one-step electrodeposition method. The morphologies of modified electrodes could be easily controlled by changing the pH of HAuCl
4 solution. The novel nanoflower-like particles with the nanoplates as the building blocks could be interestingly obtained at pH 5.0. The gold nanoflower modified electrodes were then used for the fabrication of electrochemical DNA biosensor. The DNA biosensor fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the use of ferricyanide as an electrochemical redox indicator. The DNA immobilization and hybridization on gold nanoflower modified electrode was studied with the use of [Ru(NH
3)
6]
3+ as a hybridization indicator. The electrochemical DNA biosensor shows a good selectivity and sensitivity toward the detection of target DNA. A detection limit of 1
pM toward target DNA could be obtained. |
| doi_str_mv | 10.1016/j.bios.2011.09.003 |
| format | Article |
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4 solution. The novel nanoflower-like particles with the nanoplates as the building blocks could be interestingly obtained at pH 5.0. The gold nanoflower modified electrodes were then used for the fabrication of electrochemical DNA biosensor. The DNA biosensor fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the use of ferricyanide as an electrochemical redox indicator. The DNA immobilization and hybridization on gold nanoflower modified electrode was studied with the use of [Ru(NH
3)
6]
3+ as a hybridization indicator. The electrochemical DNA biosensor shows a good selectivity and sensitivity toward the detection of target DNA. A detection limit of 1
pM toward target DNA could be obtained.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2011.09.003</identifier><identifier>PMID: 21963391</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensors ; Biotechnology ; Conductometry - instrumentation ; DNA - analysis ; DNA - genetics ; DNA hybridization ; DNA immobilization ; Electrochemical biosensor ; Electrodeposition ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; General aspects ; Gold - chemistry ; Gold nanostructure ; Immobilization techniques ; Methods. Procedures. Technologies ; Nanostructures - chemistry ; Nanotechnology - instrumentation ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2011-12, Vol.30 (1), p.151-157</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-dee065083b04733d8ac18622e1d74f0e159d4e5d3cf2cdfefba4f016fc43e2973</citedby><cites>FETCH-LOGICAL-c417t-dee065083b04733d8ac18622e1d74f0e159d4e5d3cf2cdfefba4f016fc43e2973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2011.09.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25923238$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21963391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Chen, Xiaohong</creatorcontrib><creatorcontrib>Wang, Xiaoli</creatorcontrib><creatorcontrib>Han, Xiaoping</creatorcontrib><creatorcontrib>Liu, Shufeng</creatorcontrib><creatorcontrib>Zhao, Changzhi</creatorcontrib><title>Electrochemical synthesis of gold nanostructure modified electrode and its development in electrochemical DNA biosensor</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>In this article, gold nanostructure modified electrodes were achieved by a simple one-step electrodeposition method. The morphologies of modified electrodes could be easily controlled by changing the pH of HAuCl
4 solution. The novel nanoflower-like particles with the nanoplates as the building blocks could be interestingly obtained at pH 5.0. The gold nanoflower modified electrodes were then used for the fabrication of electrochemical DNA biosensor. The DNA biosensor fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the use of ferricyanide as an electrochemical redox indicator. The DNA immobilization and hybridization on gold nanoflower modified electrode was studied with the use of [Ru(NH
3)
6]
3+ as a hybridization indicator. The electrochemical DNA biosensor shows a good selectivity and sensitivity toward the detection of target DNA. A detection limit of 1
pM toward target DNA could be obtained.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Conductometry - instrumentation</subject><subject>DNA - analysis</subject><subject>DNA - genetics</subject><subject>DNA hybridization</subject><subject>DNA immobilization</subject><subject>Electrochemical biosensor</subject><subject>Electrodeposition</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Gold - chemistry</subject><subject>Gold nanostructure</subject><subject>Immobilization techniques</subject><subject>Methods. Procedures. 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Psychology</topic><topic>General aspects</topic><topic>Gold - chemistry</topic><topic>Gold nanostructure</topic><topic>Immobilization techniques</topic><topic>Methods. Procedures. Technologies</topic><topic>Nanostructures - chemistry</topic><topic>Nanotechnology - instrumentation</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Chen, Xiaohong</creatorcontrib><creatorcontrib>Wang, Xiaoli</creatorcontrib><creatorcontrib>Han, Xiaoping</creatorcontrib><creatorcontrib>Liu, Shufeng</creatorcontrib><creatorcontrib>Zhao, Changzhi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Li</au><au>Chen, Xiaohong</au><au>Wang, Xiaoli</au><au>Han, Xiaoping</au><au>Liu, Shufeng</au><au>Zhao, Changzhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical synthesis of gold nanostructure modified electrode and its development in electrochemical DNA biosensor</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2011-12-15</date><risdate>2011</risdate><volume>30</volume><issue>1</issue><spage>151</spage><epage>157</epage><pages>151-157</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>In this article, gold nanostructure modified electrodes were achieved by a simple one-step electrodeposition method. The morphologies of modified electrodes could be easily controlled by changing the pH of HAuCl
4 solution. The novel nanoflower-like particles with the nanoplates as the building blocks could be interestingly obtained at pH 5.0. The gold nanoflower modified electrodes were then used for the fabrication of electrochemical DNA biosensor. The DNA biosensor fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the use of ferricyanide as an electrochemical redox indicator. The DNA immobilization and hybridization on gold nanoflower modified electrode was studied with the use of [Ru(NH
3)
6]
3+ as a hybridization indicator. The electrochemical DNA biosensor shows a good selectivity and sensitivity toward the detection of target DNA. A detection limit of 1
pM toward target DNA could be obtained.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>21963391</pmid><doi>10.1016/j.bios.2011.09.003</doi><tpages>7</tpages></addata></record> |
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| subjects | Biological and medical sciences Biosensing Techniques - instrumentation Biosensors Biotechnology Conductometry - instrumentation DNA - analysis DNA - genetics DNA hybridization DNA immobilization Electrochemical biosensor Electrodeposition Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology General aspects Gold - chemistry Gold nanostructure Immobilization techniques Methods. Procedures. Technologies Nanostructures - chemistry Nanotechnology - instrumentation Various methods and equipments |
| title | Electrochemical synthesis of gold nanostructure modified electrode and its development in electrochemical DNA biosensor |
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