Deletion of the aceE gene (encoding a component of pyruvate dehydrogenase) attenuates Salmonella enterica serovar Enteritidis
Abstract Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major food-borne pathogen. From a transposon insertion mutant library created previously using S. Enteritidis 10/02, one of the mutants was identified to have a 50% lethal dose (LD50) at least 100 times that of the parental strai...
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| Veröffentlicht in: | FEMS immunology and medical microbiology 2011-10, Vol.63 (1), p.108-118 |
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| creator | Pang, Ervinna Tien-Lin, Chang Selvaraj, Madhan Chang, Jason Kwang, Jimmy |
| description | Abstract
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major food-borne pathogen. From a transposon insertion mutant library created previously using S. Enteritidis 10/02, one of the mutants was identified to have a 50% lethal dose (LD50) at least 100 times that of the parental strain in young chicks, with an attenuation in a poorly studied gene encoding a component of pyruvate dehydrogenase, namely the aceE gene. Evaluation of the in vitro virulence characteristics of the ΔaceE∷kan mutant revealed that it was less able to invade epithelial cells, less resistant to reactive oxygen intermediate, less able to survive within a chicken macrophage cell line and had a retarded growth rate compared with the parental strain. Young chicks vaccinated with 2 × 109 CFU of the ΔaceE∷kan mutant were protected from the subsequent challenge of the parental strain, with the mutant colonized in the liver and spleen in a shorter time than the group infected with the parental strain. In addition, compared with the parental strain, the ΔaceE∷kan mutant did not cause persistent eggshell contamination of vaccinated hens. |
| doi_str_mv | 10.1111/j.1574-695X.2011.00834.x |
| format | Article |
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Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major food-borne pathogen. From a transposon insertion mutant library created previously using S. Enteritidis 10/02, one of the mutants was identified to have a 50% lethal dose (LD50) at least 100 times that of the parental strain in young chicks, with an attenuation in a poorly studied gene encoding a component of pyruvate dehydrogenase, namely the aceE gene. Evaluation of the in vitro virulence characteristics of the ΔaceE∷kan mutant revealed that it was less able to invade epithelial cells, less resistant to reactive oxygen intermediate, less able to survive within a chicken macrophage cell line and had a retarded growth rate compared with the parental strain. Young chicks vaccinated with 2 × 109 CFU of the ΔaceE∷kan mutant were protected from the subsequent challenge of the parental strain, with the mutant colonized in the liver and spleen in a shorter time than the group infected with the parental strain. In addition, compared with the parental strain, the ΔaceE∷kan mutant did not cause persistent eggshell contamination of vaccinated hens.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1111/j.1574-695X.2011.00834.x</identifier><identifier>PMID: 21707778</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>aceE gene ; Animals ; Animals, Newborn ; Anti-Bacterial Agents - toxicity ; Attenuation ; Cells, Cultured ; Chickens ; Chicks ; Clonal deletion ; Contamination ; Dehydrogenase ; Dehydrogenases ; DNA Transposable Elements ; Egg shells ; Epithelial cells ; Epithelial Cells - microbiology ; Food ; Food contamination ; Gene Deletion ; Growth rate ; Insertion ; Juveniles ; Lethal dose ; Lethal Dose 50 ; Liver ; Liver - microbiology ; Macrophages ; Macrophages - microbiology ; Microbial Viability - drug effects ; Mutagenesis, Insertional ; mutant ; Mutants ; Pathogens ; Pyruvate Dehydrogenase Complex - genetics ; Pyruvate Dehydrogenase Complex - metabolism ; Pyruvic acid ; Reactive oxygen species ; Reactive Oxygen Species - toxicity ; Salmonella ; Salmonella enterica ; Salmonella Enteritidis ; Salmonella enteritidis - drug effects ; Salmonella enteritidis - enzymology ; Salmonella enteritidis - growth & development ; Salmonella enteritidis - pathogenicity ; Spleen ; Spleen - microbiology ; Survival Analysis ; Transposons ; Vaccination ; vaccine ; Virulence ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>FEMS immunology and medical microbiology, 2011-10, Vol.63 (1), p.108-118</ispartof><rights>2011 Federation of European Microbiological Societies 2011</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><rights>2011 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4014-d5697ff32453f98ed254f7f8f64eb7be0997a412dad6e6923d61574ce3f27603</citedby><cites>FETCH-LOGICAL-c4014-d5697ff32453f98ed254f7f8f64eb7be0997a412dad6e6923d61574ce3f27603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-695X.2011.00834.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-695X.2011.00834.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21707778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pang, Ervinna</creatorcontrib><creatorcontrib>Tien-Lin, Chang</creatorcontrib><creatorcontrib>Selvaraj, Madhan</creatorcontrib><creatorcontrib>Chang, Jason</creatorcontrib><creatorcontrib>Kwang, Jimmy</creatorcontrib><title>Deletion of the aceE gene (encoding a component of pyruvate dehydrogenase) attenuates Salmonella enterica serovar Enteritidis</title><title>FEMS immunology and medical microbiology</title><addtitle>FEMS Immunol Med Microbiol</addtitle><description>Abstract
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major food-borne pathogen. From a transposon insertion mutant library created previously using S. Enteritidis 10/02, one of the mutants was identified to have a 50% lethal dose (LD50) at least 100 times that of the parental strain in young chicks, with an attenuation in a poorly studied gene encoding a component of pyruvate dehydrogenase, namely the aceE gene. Evaluation of the in vitro virulence characteristics of the ΔaceE∷kan mutant revealed that it was less able to invade epithelial cells, less resistant to reactive oxygen intermediate, less able to survive within a chicken macrophage cell line and had a retarded growth rate compared with the parental strain. Young chicks vaccinated with 2 × 109 CFU of the ΔaceE∷kan mutant were protected from the subsequent challenge of the parental strain, with the mutant colonized in the liver and spleen in a shorter time than the group infected with the parental strain. In addition, compared with the parental strain, the ΔaceE∷kan mutant did not cause persistent eggshell contamination of vaccinated hens.</description><subject>aceE gene</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Anti-Bacterial Agents - toxicity</subject><subject>Attenuation</subject><subject>Cells, Cultured</subject><subject>Chickens</subject><subject>Chicks</subject><subject>Clonal deletion</subject><subject>Contamination</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>DNA Transposable Elements</subject><subject>Egg shells</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - microbiology</subject><subject>Food</subject><subject>Food contamination</subject><subject>Gene Deletion</subject><subject>Growth rate</subject><subject>Insertion</subject><subject>Juveniles</subject><subject>Lethal dose</subject><subject>Lethal Dose 50</subject><subject>Liver</subject><subject>Liver - microbiology</subject><subject>Macrophages</subject><subject>Macrophages - microbiology</subject><subject>Microbial Viability - drug effects</subject><subject>Mutagenesis, Insertional</subject><subject>mutant</subject><subject>Mutants</subject><subject>Pathogens</subject><subject>Pyruvate Dehydrogenase Complex - genetics</subject><subject>Pyruvate Dehydrogenase Complex - metabolism</subject><subject>Pyruvic acid</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - toxicity</subject><subject>Salmonella</subject><subject>Salmonella enterica</subject><subject>Salmonella Enteritidis</subject><subject>Salmonella enteritidis - drug effects</subject><subject>Salmonella enteritidis - enzymology</subject><subject>Salmonella enteritidis - growth & development</subject><subject>Salmonella enteritidis - pathogenicity</subject><subject>Spleen</subject><subject>Spleen - microbiology</subject><subject>Survival Analysis</subject><subject>Transposons</subject><subject>Vaccination</subject><subject>vaccine</subject><subject>Virulence</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS1ERYfCKyBLLIBFgv9ixxIb1E6hUhELumBneeLrNqMkDnbSdha8O06n7QKEwBtbvt-5Pr4HIUxJSfN6vy1ppUQhdfW9ZITSkpCai_L2CVo9Fp6iFdGsLmomxCF6ntKWECI0Ic_QIaOKKKXqFfp5Ah1MbRhw8Hi6AmwbWONLGAC_haEJrh0uscVN6McwwDAt2LiL87WdADu42rkYMm0TvMN2mmCYcyHhb7brM991FmcRxLaxOEEM1zbi9d3F1Lo2vUAH3nYJXt7vR-jidH1x_Lk4__rp7PjjedEIQkXhKqmV95yJintdg2OV8MrXXgrYqA0QrZUVlDnrJEjNuJPLGBrgnilJ-BF6s287xvBjhjSZvk3N4m6AMCej80wrWQn-T7Ku85wpq3QmX_9GbsMch_wLwzhRVHItq0zVe6qJIaUI3oyx7W3cGUrMEqXZmsWqWRIzS5TmLkpzm6Wv7h-YNz24R-FDdhn4sAdu2g52_93YnJ59yYcs53t5mMe_iIs_Xf0Cnee80A</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Pang, Ervinna</creator><creator>Tien-Lin, Chang</creator><creator>Selvaraj, Madhan</creator><creator>Chang, Jason</creator><creator>Kwang, Jimmy</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20111001</creationdate><title>Deletion of the aceE gene (encoding a component of pyruvate dehydrogenase) attenuates Salmonella enterica serovar Enteritidis</title><author>Pang, Ervinna ; Tien-Lin, Chang ; Selvaraj, Madhan ; Chang, Jason ; Kwang, Jimmy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4014-d5697ff32453f98ed254f7f8f64eb7be0997a412dad6e6923d61574ce3f27603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>aceE gene</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Anti-Bacterial Agents - toxicity</topic><topic>Attenuation</topic><topic>Cells, Cultured</topic><topic>Chickens</topic><topic>Chicks</topic><topic>Clonal deletion</topic><topic>Contamination</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>DNA Transposable Elements</topic><topic>Egg shells</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - microbiology</topic><topic>Food</topic><topic>Food contamination</topic><topic>Gene Deletion</topic><topic>Growth rate</topic><topic>Insertion</topic><topic>Juveniles</topic><topic>Lethal dose</topic><topic>Lethal Dose 50</topic><topic>Liver</topic><topic>Liver - microbiology</topic><topic>Macrophages</topic><topic>Macrophages - microbiology</topic><topic>Microbial Viability - drug effects</topic><topic>Mutagenesis, Insertional</topic><topic>mutant</topic><topic>Mutants</topic><topic>Pathogens</topic><topic>Pyruvate Dehydrogenase Complex - genetics</topic><topic>Pyruvate Dehydrogenase Complex - metabolism</topic><topic>Pyruvic acid</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - toxicity</topic><topic>Salmonella</topic><topic>Salmonella enterica</topic><topic>Salmonella Enteritidis</topic><topic>Salmonella enteritidis - drug effects</topic><topic>Salmonella enteritidis - enzymology</topic><topic>Salmonella enteritidis - growth & development</topic><topic>Salmonella enteritidis - pathogenicity</topic><topic>Spleen</topic><topic>Spleen - microbiology</topic><topic>Survival Analysis</topic><topic>Transposons</topic><topic>Vaccination</topic><topic>vaccine</topic><topic>Virulence</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pang, Ervinna</creatorcontrib><creatorcontrib>Tien-Lin, Chang</creatorcontrib><creatorcontrib>Selvaraj, Madhan</creatorcontrib><creatorcontrib>Chang, Jason</creatorcontrib><creatorcontrib>Kwang, Jimmy</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pang, Ervinna</au><au>Tien-Lin, Chang</au><au>Selvaraj, Madhan</au><au>Chang, Jason</au><au>Kwang, Jimmy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deletion of the aceE gene (encoding a component of pyruvate dehydrogenase) attenuates Salmonella enterica serovar Enteritidis</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><addtitle>FEMS Immunol Med Microbiol</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>63</volume><issue>1</issue><spage>108</spage><epage>118</epage><pages>108-118</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>Abstract
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major food-borne pathogen. From a transposon insertion mutant library created previously using S. Enteritidis 10/02, one of the mutants was identified to have a 50% lethal dose (LD50) at least 100 times that of the parental strain in young chicks, with an attenuation in a poorly studied gene encoding a component of pyruvate dehydrogenase, namely the aceE gene. Evaluation of the in vitro virulence characteristics of the ΔaceE∷kan mutant revealed that it was less able to invade epithelial cells, less resistant to reactive oxygen intermediate, less able to survive within a chicken macrophage cell line and had a retarded growth rate compared with the parental strain. Young chicks vaccinated with 2 × 109 CFU of the ΔaceE∷kan mutant were protected from the subsequent challenge of the parental strain, with the mutant colonized in the liver and spleen in a shorter time than the group infected with the parental strain. In addition, compared with the parental strain, the ΔaceE∷kan mutant did not cause persistent eggshell contamination of vaccinated hens.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21707778</pmid><doi>10.1111/j.1574-695X.2011.00834.x</doi><tpages>11</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals |
| subjects | aceE gene Animals Animals, Newborn Anti-Bacterial Agents - toxicity Attenuation Cells, Cultured Chickens Chicks Clonal deletion Contamination Dehydrogenase Dehydrogenases DNA Transposable Elements Egg shells Epithelial cells Epithelial Cells - microbiology Food Food contamination Gene Deletion Growth rate Insertion Juveniles Lethal dose Lethal Dose 50 Liver Liver - microbiology Macrophages Macrophages - microbiology Microbial Viability - drug effects Mutagenesis, Insertional mutant Mutants Pathogens Pyruvate Dehydrogenase Complex - genetics Pyruvate Dehydrogenase Complex - metabolism Pyruvic acid Reactive oxygen species Reactive Oxygen Species - toxicity Salmonella Salmonella enterica Salmonella Enteritidis Salmonella enteritidis - drug effects Salmonella enteritidis - enzymology Salmonella enteritidis - growth & development Salmonella enteritidis - pathogenicity Spleen Spleen - microbiology Survival Analysis Transposons Vaccination vaccine Virulence Virulence Factors - genetics Virulence Factors - metabolism |
| title | Deletion of the aceE gene (encoding a component of pyruvate dehydrogenase) attenuates Salmonella enterica serovar Enteritidis |
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