Avian glycan-specific IgM monoclonal antibodies for the detection and quantitation of type A and B haemagglutinins in egg-derived influenza vaccines

► Two Mabs which are of particular interest to detect and quantify HA in egg influenza vaccines were characterised. ► Two ELISAs were set up, one is strain specific, the other is a universal influenza ELISA. ► These ELISAs are of particular interest for the quantitation of HA in egg-grown pandemic a...

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Veröffentlicht in:	Journal of virological methods 2011-12, Vol.178 (1-2), p.129-136
Hauptverfasser:	Legastelois, Isabelle, Chevalier, Michel, Bernard, Marie-Clotilde, de Montfort, Aymeric, Fouque, Martine, Pilloud, Alexandra, Serraille, Christelle, Devard, Nicolas, Engel, Olivier, Sodoyer, Régis, Moste, Catherine
Format:	Artikel
Sprache:	eng
Schlagworte:	adjuvants Animals Antibodies, Monoclonal Antigens, Viral - analysis Antigens, Viral - immunology birds clones detection limit ELISA enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Hemagglutinin Glycoproteins, Influenza Virus - analysis Hemagglutinin Glycoproteins, Influenza Virus - immunology hemagglutinins IgM Immunodiffusion - methods Immunoglobulin M Influenza Influenza A virus - growth & development Influenza A virus - immunology Influenza Vaccines - chemistry Influenza Vaccines - immunology Mice Mice, Inbred BALB C Monoclonal antibodies pandemic polysaccharides Polysaccharides - immunology screening SRID Universal influenza reagent vaccines viruses
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