Analysis of Iranian Potato virus S isolates

Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) us...

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Veröffentlicht in:Virus genes 2011-10, Vol.43 (2), p.281-288
Hauptverfasser: Salari, Khadijeh, Massumi, Hossein, Heydarnejad, Jahangir, Hosseini Pour, Akbar, Varsani, Arvind
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creator Salari, Khadijeh
Massumi, Hossein
Heydarnejad, Jahangir
Hosseini Pour, Akbar
Varsani, Arvind
description Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using a Potato virus S (PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on Nicotiana debneyii and N. tabacum cv. Whiteburly and local lesion on Chenopodium amaranticolor, C. quinoa and C. album . The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS O isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes for the PVS O strain and 3 genotypes for the PVS A strain.
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Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using a Potato virus S (PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on Nicotiana debneyii and N. tabacum cv. Whiteburly and local lesion on Chenopodium amaranticolor, C. quinoa and C. album . The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS O isolates. 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subjects Biomedical and Life Sciences
Biomedicine
Carlavirus - classification
Carlavirus - genetics
Carlavirus - isolation & purification
Chenopodium
Genes, Viral
Genotype
Host Specificity - physiology
Iran
Medical Microbiology
Molecular Sequence Data
Nicotiana
Phylogeny
Plant Leaves - virology
Plant Sciences
Potato virus S
Sequence Homology
Solanum tuberosum
Solanum tuberosum - virology
Virology
title Analysis of Iranian Potato virus S isolates
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