Analysis of Iranian Potato virus S isolates
Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) us...
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creator | Salari, Khadijeh Massumi, Hossein Heydarnejad, Jahangir Hosseini Pour, Akbar Varsani, Arvind |
description | Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using a
Potato virus S
(PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on
Nicotiana debneyii
and
N. tabacum
cv. Whiteburly and local lesion on
Chenopodium amaranticolor, C. quinoa
and
C. album
. The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS
O
isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes for the PVS
O
strain and 3 genotypes for the PVS
A
strain. |
doi_str_mv | 10.1007/s11262-011-0619-3 |
format | Article |
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Potato virus S
(PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on
Nicotiana debneyii
and
N. tabacum
cv. Whiteburly and local lesion on
Chenopodium amaranticolor, C. quinoa
and
C. album
. The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS
O
isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes for the PVS
O
strain and 3 genotypes for the PVS
A
strain.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-011-0619-3</identifier><identifier>PMID: 21567245</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Carlavirus - classification ; Carlavirus - genetics ; Carlavirus - isolation & purification ; Chenopodium ; Genes, Viral ; Genotype ; Host Specificity - physiology ; Iran ; Medical Microbiology ; Molecular Sequence Data ; Nicotiana ; Phylogeny ; Plant Leaves - virology ; Plant Sciences ; Potato virus S ; Sequence Homology ; Solanum tuberosum ; Solanum tuberosum - virology ; Virology</subject><ispartof>Virus genes, 2011-10, Vol.43 (2), p.281-288</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-33462dfa8994bab83d7f8989dd3beb34f77bb4bc6caf42214479f290547eeb0b3</citedby><cites>FETCH-LOGICAL-c402t-33462dfa8994bab83d7f8989dd3beb34f77bb4bc6caf42214479f290547eeb0b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11262-011-0619-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11262-011-0619-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21567245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salari, Khadijeh</creatorcontrib><creatorcontrib>Massumi, Hossein</creatorcontrib><creatorcontrib>Heydarnejad, Jahangir</creatorcontrib><creatorcontrib>Hosseini Pour, Akbar</creatorcontrib><creatorcontrib>Varsani, Arvind</creatorcontrib><title>Analysis of Iranian Potato virus S isolates</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><addtitle>Virus Genes</addtitle><description>Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using a
Potato virus S
(PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on
Nicotiana debneyii
and
N. tabacum
cv. Whiteburly and local lesion on
Chenopodium amaranticolor, C. quinoa
and
C. album
. The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS
O
isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes for the PVS
O
strain and 3 genotypes for the PVS
A
strain.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Carlavirus - classification</subject><subject>Carlavirus - genetics</subject><subject>Carlavirus - isolation & purification</subject><subject>Chenopodium</subject><subject>Genes, Viral</subject><subject>Genotype</subject><subject>Host Specificity - physiology</subject><subject>Iran</subject><subject>Medical Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana</subject><subject>Phylogeny</subject><subject>Plant Leaves - virology</subject><subject>Plant Sciences</subject><subject>Potato virus S</subject><subject>Sequence Homology</subject><subject>Solanum tuberosum</subject><subject>Solanum tuberosum - virology</subject><subject>Virology</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkE1Lw0AQhhdRbK3-AC8SvHiQ1dmv7O6xFD8KBQUVvC27yUZS0qTuJkL_vSmpCoJ4msM8887Mg9ApgSsCIK8jITSlGAjBkBKN2R4aEyEp1pq_7qMxaApYiVSP0FGMSwBQivJDNKJEpJJyMUaX09pWm1jGpCmSebB1aevksWlt2yQfZehi8pSUsals6-MxOihsFf3Jrk7Qy-3N8-weLx7u5rPpAmccaIsZ4ynNC6v6K5x1iuWyUFrpPGfOO8YLKZ3jLkszW3BKCedSF1SD4NJ7B45N0MWQuw7Ne-dja1ZlzHxV2do3XTSaQCpAaPiXVIpTSTglPXn-i1w2Xeh_30JMpVoK2UNkgLLQxBh8YdahXNmwMQTM1rgZjJveuNkaN6yfOdsFd27l8--JL8U9QAcg9q36zYefzX-nfgJYaoko</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Salari, Khadijeh</creator><creator>Massumi, Hossein</creator><creator>Heydarnejad, Jahangir</creator><creator>Hosseini Pour, Akbar</creator><creator>Varsani, Arvind</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20111001</creationdate><title>Analysis of Iranian Potato virus S isolates</title><author>Salari, Khadijeh ; Massumi, Hossein ; Heydarnejad, Jahangir ; Hosseini Pour, Akbar ; Varsani, Arvind</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-33462dfa8994bab83d7f8989dd3beb34f77bb4bc6caf42214479f290547eeb0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Carlavirus - classification</topic><topic>Carlavirus - genetics</topic><topic>Carlavirus - isolation & purification</topic><topic>Chenopodium</topic><topic>Genes, Viral</topic><topic>Genotype</topic><topic>Host Specificity - physiology</topic><topic>Iran</topic><topic>Medical Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana</topic><topic>Phylogeny</topic><topic>Plant Leaves - virology</topic><topic>Plant Sciences</topic><topic>Potato virus S</topic><topic>Sequence Homology</topic><topic>Solanum tuberosum</topic><topic>Solanum tuberosum - virology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salari, Khadijeh</creatorcontrib><creatorcontrib>Massumi, Hossein</creatorcontrib><creatorcontrib>Heydarnejad, Jahangir</creatorcontrib><creatorcontrib>Hosseini Pour, Akbar</creatorcontrib><creatorcontrib>Varsani, Arvind</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salari, Khadijeh</au><au>Massumi, Hossein</au><au>Heydarnejad, Jahangir</au><au>Hosseini Pour, Akbar</au><au>Varsani, Arvind</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Iranian Potato virus S isolates</atitle><jtitle>Virus genes</jtitle><stitle>Virus Genes</stitle><addtitle>Virus Genes</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>43</volume><issue>2</issue><spage>281</spage><epage>288</epage><pages>281-288</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>Two hundred forty potato samples with one or more symptoms of leaf mosaic, distortion, mottling and yellowing were collected between 2005 and 2008 from seven Iranian provinces. Forty-four of these samples tested positive with double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using a
Potato virus S
(PVS) polyclonal antibody. Of these 12 isolates of PVS were selected based on the geographical location for biological and molecular characterization. The full coat protein (CP) and 11K genes from 12 PVS isolates were PCR amplified, cloned and sequenced. All 12 PVS isolates showed mosaic symptoms on
Nicotiana debneyii
and
N. tabacum
cv. Whiteburly and local lesion on
Chenopodium amaranticolor, C. quinoa
and
C. album
. The Iranian isolates share between 93 and 100% pairwise nucleotide identity with other PVS
O
isolates. Based on maximum likelihood phylogenetic analysis coupled with pairwise identity analysis, we propose 15 genotypes for the PVS
O
strain and 3 genotypes for the PVS
A
strain.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>21567245</pmid><doi>10.1007/s11262-011-0619-3</doi><tpages>8</tpages></addata></record> |
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subjects | Biomedical and Life Sciences Biomedicine Carlavirus - classification Carlavirus - genetics Carlavirus - isolation & purification Chenopodium Genes, Viral Genotype Host Specificity - physiology Iran Medical Microbiology Molecular Sequence Data Nicotiana Phylogeny Plant Leaves - virology Plant Sciences Potato virus S Sequence Homology Solanum tuberosum Solanum tuberosum - virology Virology |
title | Analysis of Iranian Potato virus S isolates |
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