Measurement of Glucose Uptake in Cultured Cells
Facilitative glucose uptake transport systems are ubiquitous in animal cells and responsible for transporting glucose across the cell surface membrane. Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders, such as myocardial ischemia, diabetes mellitus, a...
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| Veröffentlicht in: | Current Protocols in Pharmacology 2011-12, Vol.55 (1), p.12.14.1-12.14.22 |
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| container_end_page | 12.14.22 |
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| container_issue | 1 |
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| container_title | Current Protocols in Pharmacology |
| container_volume | 55 |
| creator | Yamamoto, Norio Ueda, Manabu Sato, Takuya Kawasaki, Kengo Sawada, Keisuke Kawabata, Kyuichi Ashida, Hitoshi |
| description | Facilitative glucose uptake transport systems are ubiquitous in animal cells and responsible for transporting glucose across the cell surface membrane. Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders, such as myocardial ischemia, diabetes mellitus, and cancer. Methods for assessing glucose uptake into mammalian cells are detailed in this unit. The work is divided into four sections: (1) a brief overview of glucose uptake assays in cultured cells; (2) a method for measuring glucose uptake using radiolabeled 3‐O‐methylglucose; (3) a method for measuring glucose uptake using radiolabeled 2‐deoxyglucose (2DG); and (4) an improved method for measuring 2DG‐uptake using an enzymatic, fluorometric assay, eliminating the need for radiolabeled glucose analogs. Curr. Protoc. Pharmacol. 55:12.14.1‐12.14.22. © 2011 by John Wiley & Sons, Inc. |
| doi_str_mv | 10.1002/0471141755.ph1214s55 |
| format | Article |
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Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders, such as myocardial ischemia, diabetes mellitus, and cancer. Methods for assessing glucose uptake into mammalian cells are detailed in this unit. The work is divided into four sections: (1) a brief overview of glucose uptake assays in cultured cells; (2) a method for measuring glucose uptake using radiolabeled 3‐O‐methylglucose; (3) a method for measuring glucose uptake using radiolabeled 2‐deoxyglucose (2DG); and (4) an improved method for measuring 2DG‐uptake using an enzymatic, fluorometric assay, eliminating the need for radiolabeled glucose analogs. Curr. Protoc. 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Pharmacol. 55:12.14.1‐12.14.22. © 2011 by John Wiley & Sons, Inc.</description><subject>2‐deoxyglucose</subject><subject>3-O-Methylglucose - analysis</subject><subject>3-O-Methylglucose - pharmacokinetics</subject><subject>3‐O‐methylglucose</subject><subject>Adipocytes - metabolism</subject><subject>Animals</subject><subject>Biological Assay - methods</subject><subject>cells</subject><subject>Cells, Cultured</subject><subject>Deoxyglucose - analysis</subject><subject>Deoxyglucose - pharmacokinetics</subject><subject>Facilitated Diffusion - physiology</subject><subject>Glucose Transport Proteins, Facilitative - metabolism</subject><subject>glucose uptake</subject><subject>Homeostasis - physiology</subject><subject>Humans</subject><subject>Mice</subject><subject>Muscle Cells - metabolism</subject><subject>Muscle, Skeletal - metabolism</subject><subject>nonradioisotope</subject><subject>radioisotope</subject><subject>Radioligand Assay - methods</subject><subject>Rats</subject><issn>1934-8282</issn><issn>1934-8290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1Lw0AQhhdRbKn9ByK5eUo7-9Xd9SZBW6FiD_a8bJIJBpMmZhOk_94trfXqaebwPO8MLyG3FGYUgM1BKEoFVVLO2g_KqPBSXpAxNVzEmhm4PO-ajcjU-zIFEAuxCNI1GbFgKC7UmMxf0fmhwxp3fdQU0bIassZjtG1794lRuYuSoeoDkEcJVpW_IVeFqzxOT3NCts9P78kqXr8tX5LHdZwJ0CIuuKGSyRS0ExywQBAGpMtSpQ3kxqWGI3PCGck16PAacywXhZYLDIoq-ITcH3Pbrvka0Pe2Ln0WPnA7bAZvDRgludAykOJIZl3jfYeFbbuydt3eUrCHsuxfWfZcVtDuTgeGtMb8LP1WE4CHI_BdVrj_V6hNNpvVYec_WdF0JA</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Yamamoto, Norio</creator><creator>Ueda, Manabu</creator><creator>Sato, Takuya</creator><creator>Kawasaki, Kengo</creator><creator>Sawada, Keisuke</creator><creator>Kawabata, Kyuichi</creator><creator>Ashida, Hitoshi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201112</creationdate><title>Measurement of Glucose Uptake in Cultured Cells</title><author>Yamamoto, Norio ; Ueda, Manabu ; Sato, Takuya ; Kawasaki, Kengo ; Sawada, Keisuke ; Kawabata, Kyuichi ; Ashida, Hitoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4084-f391525b08a430efe04905acb7890d9ab93e2a4a9538080042a2d4f856e08a7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>2‐deoxyglucose</topic><topic>3-O-Methylglucose - analysis</topic><topic>3-O-Methylglucose - pharmacokinetics</topic><topic>3‐O‐methylglucose</topic><topic>Adipocytes - metabolism</topic><topic>Animals</topic><topic>Biological Assay - methods</topic><topic>cells</topic><topic>Cells, Cultured</topic><topic>Deoxyglucose - analysis</topic><topic>Deoxyglucose - pharmacokinetics</topic><topic>Facilitated Diffusion - physiology</topic><topic>Glucose Transport Proteins, Facilitative - metabolism</topic><topic>glucose uptake</topic><topic>Homeostasis - physiology</topic><topic>Humans</topic><topic>Mice</topic><topic>Muscle Cells - metabolism</topic><topic>Muscle, Skeletal - metabolism</topic><topic>nonradioisotope</topic><topic>radioisotope</topic><topic>Radioligand Assay - methods</topic><topic>Rats</topic><toplevel>online_resources</toplevel><creatorcontrib>Yamamoto, Norio</creatorcontrib><creatorcontrib>Ueda, Manabu</creatorcontrib><creatorcontrib>Sato, Takuya</creatorcontrib><creatorcontrib>Kawasaki, Kengo</creatorcontrib><creatorcontrib>Sawada, Keisuke</creatorcontrib><creatorcontrib>Kawabata, Kyuichi</creatorcontrib><creatorcontrib>Ashida, Hitoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Current Protocols in Pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamamoto, Norio</au><au>Ueda, Manabu</au><au>Sato, Takuya</au><au>Kawasaki, Kengo</au><au>Sawada, Keisuke</au><au>Kawabata, Kyuichi</au><au>Ashida, Hitoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of Glucose Uptake in Cultured Cells</atitle><jtitle>Current Protocols in Pharmacology</jtitle><addtitle>Curr Protoc Pharmacol</addtitle><date>2011-12</date><risdate>2011</risdate><volume>55</volume><issue>1</issue><spage>12.14.1</spage><epage>12.14.22</epage><pages>12.14.1-12.14.22</pages><issn>1934-8282</issn><eissn>1934-8290</eissn><abstract>Facilitative glucose uptake transport systems are ubiquitous in animal cells and responsible for transporting glucose across the cell surface membrane. 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| identifier | ISSN: 1934-8282 |
| ispartof | Current Protocols in Pharmacology, 2011-12, Vol.55 (1), p.12.14.1-12.14.22 |
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| subjects | 2‐deoxyglucose 3-O-Methylglucose - analysis 3-O-Methylglucose - pharmacokinetics 3‐O‐methylglucose Adipocytes - metabolism Animals Biological Assay - methods cells Cells, Cultured Deoxyglucose - analysis Deoxyglucose - pharmacokinetics Facilitated Diffusion - physiology Glucose Transport Proteins, Facilitative - metabolism glucose uptake Homeostasis - physiology Humans Mice Muscle Cells - metabolism Muscle, Skeletal - metabolism nonradioisotope radioisotope Radioligand Assay - methods Rats |
| title | Measurement of Glucose Uptake in Cultured Cells |
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