A rapid and sensitive method for the analysis of brain monoamine neurotransmitters using ultra-fast liquid chromatography coupled to electrochemical detection
► Catecholamines and indolamines are the most studied monoamines in neuroscience. ► Time of routine analyses by conventional HPLC and electrochemical detection is too long. ► Use of sub-2 μm particles increases the throughput of analysis using ultra-fast HPLC. ► Brain monoamines can be determined in...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-12, Vol.879 (32), p.3871-3878 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Parrot, Sandrine Neuzeret, Pierre-Charles Denoroy, Luc |
| description | ► Catecholamines and indolamines are the most studied monoamines in neuroscience. ► Time of routine analyses by conventional HPLC and electrochemical detection is too long. ► Use of sub-2
μm particles increases the throughput of analysis using ultra-fast HPLC. ► Brain monoamines can be determined in various tissue samples by this way. ► Potential applications of ultra-fast HPLC in brain dialysis.
Electrochemical detection is often used to detect catecholamines and indolamines in brain samples that have been separated by conventional reverse-phase high performance liquid chromatography (HPLC). This paper presents the transfer of an existing chromatographic method for the determination of monoamines in brain tissues using 5
μm granulometry HPLC columns to columns with a particle diameter less than 3
μm. Several parameters (repeatability, linearity, accuracy, limit of detection, and stability of samples) for this new ultrafast high performance liquid chromatography (UHPLC) method were examined after optimization of the analytical conditions. The separation of seven compounds, noradrenaline, dopamine and three of its metabolites, dihydroxyphenylacetic acid, homovanillic acid, and 3-methoxytyramine, and serotonin and its metabolite, 5-hydroxyindole-3-acetic acid was analyzed using this UHPLC–electrochemical detection method. The final method, which was applied to brain tissue extracts from mice, rats, and cats, decreased analysis time by a factor of 4 compared to HPLC, while guaranteeing good analytical performance. |
| doi_str_mv | 10.1016/j.jchromb.2011.10.038 |
| format | Article |
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μm particles increases the throughput of analysis using ultra-fast HPLC. ► Brain monoamines can be determined in various tissue samples by this way. ► Potential applications of ultra-fast HPLC in brain dialysis.
Electrochemical detection is often used to detect catecholamines and indolamines in brain samples that have been separated by conventional reverse-phase high performance liquid chromatography (HPLC). This paper presents the transfer of an existing chromatographic method for the determination of monoamines in brain tissues using 5
μm granulometry HPLC columns to columns with a particle diameter less than 3
μm. Several parameters (repeatability, linearity, accuracy, limit of detection, and stability of samples) for this new ultrafast high performance liquid chromatography (UHPLC) method were examined after optimization of the analytical conditions. The separation of seven compounds, noradrenaline, dopamine and three of its metabolites, dihydroxyphenylacetic acid, homovanillic acid, and 3-methoxytyramine, and serotonin and its metabolite, 5-hydroxyindole-3-acetic acid was analyzed using this UHPLC–electrochemical detection method. The final method, which was applied to brain tissue extracts from mice, rats, and cats, decreased analysis time by a factor of 4 compared to HPLC, while guaranteeing good analytical performance.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2011.10.038</identifier><identifier>PMID: 22100552</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>3,4-Dihydroxyphenylacetic Acid - analysis ; Analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Brain ; Brain Chemistry ; Brain samples ; Catecholamines - analysis ; Cats ; Chromatography ; Chromatography, High Pressure Liquid - methods ; detection limit ; dopamine ; Drug Stability ; Electrochemical detection ; electrochemistry ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; High performance liquid chromatography ; homovanillic acid ; Homovanillic Acid - analysis ; Limit of Detection ; Linearity ; Liquid chromatography ; Mathematical analysis ; Medical sciences ; Metabolite ; Metabolites ; Mice ; Monoamine ; monoamines ; Neurotransmitter Agents - analysis ; Neurotransmitters ; norepinephrine ; Pharmacology. Drug treatments ; Rats ; Reproducibility of Results ; reversed-phase high performance liquid chromatography ; serotonin ; Serotonin - analysis ; ultra-performance liquid chromatography ; Ultrafast high performance liquid chromatography ; ultrafast liquid chromatography</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-12, Vol.879 (32), p.3871-3878</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-18ee2ff1f344007a0a551fc0bb7b8fafc6b114ed80cbb5e61555abe7042fef823</citedby><cites>FETCH-LOGICAL-c517t-18ee2ff1f344007a0a551fc0bb7b8fafc6b114ed80cbb5e61555abe7042fef823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2011.10.038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25281116$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22100552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parrot, Sandrine</creatorcontrib><creatorcontrib>Neuzeret, Pierre-Charles</creatorcontrib><creatorcontrib>Denoroy, Luc</creatorcontrib><title>A rapid and sensitive method for the analysis of brain monoamine neurotransmitters using ultra-fast liquid chromatography coupled to electrochemical detection</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>► Catecholamines and indolamines are the most studied monoamines in neuroscience. ► Time of routine analyses by conventional HPLC and electrochemical detection is too long. ► Use of sub-2
μm particles increases the throughput of analysis using ultra-fast HPLC. ► Brain monoamines can be determined in various tissue samples by this way. ► Potential applications of ultra-fast HPLC in brain dialysis.
Electrochemical detection is often used to detect catecholamines and indolamines in brain samples that have been separated by conventional reverse-phase high performance liquid chromatography (HPLC). This paper presents the transfer of an existing chromatographic method for the determination of monoamines in brain tissues using 5
μm granulometry HPLC columns to columns with a particle diameter less than 3
μm. Several parameters (repeatability, linearity, accuracy, limit of detection, and stability of samples) for this new ultrafast high performance liquid chromatography (UHPLC) method were examined after optimization of the analytical conditions. The separation of seven compounds, noradrenaline, dopamine and three of its metabolites, dihydroxyphenylacetic acid, homovanillic acid, and 3-methoxytyramine, and serotonin and its metabolite, 5-hydroxyindole-3-acetic acid was analyzed using this UHPLC–electrochemical detection method. The final method, which was applied to brain tissue extracts from mice, rats, and cats, decreased analysis time by a factor of 4 compared to HPLC, while guaranteeing good analytical performance.</description><subject>3,4-Dihydroxyphenylacetic Acid - analysis</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain</subject><subject>Brain Chemistry</subject><subject>Brain samples</subject><subject>Catecholamines - analysis</subject><subject>Cats</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>detection limit</subject><subject>dopamine</subject><subject>Drug Stability</subject><subject>Electrochemical detection</subject><subject>electrochemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>High performance liquid chromatography</subject><subject>homovanillic acid</subject><subject>Homovanillic Acid - analysis</subject><subject>Limit of Detection</subject><subject>Linearity</subject><subject>Liquid chromatography</subject><subject>Mathematical analysis</subject><subject>Medical sciences</subject><subject>Metabolite</subject><subject>Metabolites</subject><subject>Mice</subject><subject>Monoamine</subject><subject>monoamines</subject><subject>Neurotransmitter Agents - analysis</subject><subject>Neurotransmitters</subject><subject>norepinephrine</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Reproducibility of Results</subject><subject>reversed-phase high performance liquid chromatography</subject><subject>serotonin</subject><subject>Serotonin - analysis</subject><subject>ultra-performance liquid chromatography</subject><subject>Ultrafast high performance liquid chromatography</subject><subject>ultrafast liquid chromatography</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O1DAQhCMEYn_gEQBf0HLJ0HbGcXJCqxV_0kocYCVukeO0Jx4l9qztrDQvw7PSwwxwg5Ot8teuVlVRvOCw4sDrt9vV1owxzP1KAOekraBqHhXnvFFVWan6-2O6SwUliEqcFRcpbQG4AlU9Lc6E4ABSivPixzWLeucGpv3AEvrksntANmMew8BsiCyPSI962ieXWLCsj9p5Ngcf9Ow8Mo9LDDlqn2aXM8bEluT8hi0TiaXVKbPJ3S9k8WtfncOGHMc9M2HZTTiwHBhOaHIMZsTZGT2xATMJLvhnxROrp4TPT-dlcffh_bebT-Xtl4-fb65vSyO5yiVvEIW13FbrNYDSoKXk1kDfq76x2pq653yNQwOm7yXWXEqpe1SwFhZtI6rL4ur47y6G-wVT7maXDE6T9hiW1LXQihZk2xL55p8klQONUlXdECqPqIkhpYi220U367gn6MDV3bY7ldgdSjzIVCLNvTxZLP2Mw5-p360R8PoE6ERxWQrfuPSXk6LhnNfEvTpyVodObyIxd1_JqQaAWvFGEvHuSCCF--Awdsk49AYHF6mBbgjuP8v-BBM-y0Y</recordid><startdate>20111215</startdate><enddate>20111215</enddate><creator>Parrot, Sandrine</creator><creator>Neuzeret, Pierre-Charles</creator><creator>Denoroy, Luc</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>7X8</scope></search><sort><creationdate>20111215</creationdate><title>A rapid and sensitive method for the analysis of brain monoamine neurotransmitters using ultra-fast liquid chromatography coupled to electrochemical detection</title><author>Parrot, Sandrine ; Neuzeret, Pierre-Charles ; Denoroy, Luc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-18ee2ff1f344007a0a551fc0bb7b8fafc6b114ed80cbb5e61555abe7042fef823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>3,4-Dihydroxyphenylacetic Acid - analysis</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain</topic><topic>Brain Chemistry</topic><topic>Brain samples</topic><topic>Catecholamines - analysis</topic><topic>Cats</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>detection limit</topic><topic>dopamine</topic><topic>Drug Stability</topic><topic>Electrochemical detection</topic><topic>electrochemistry</topic><topic>Fundamental and applied biological sciences. 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Drug treatments</topic><topic>Rats</topic><topic>Reproducibility of Results</topic><topic>reversed-phase high performance liquid chromatography</topic><topic>serotonin</topic><topic>Serotonin - analysis</topic><topic>ultra-performance liquid chromatography</topic><topic>Ultrafast high performance liquid chromatography</topic><topic>ultrafast liquid chromatography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parrot, Sandrine</creatorcontrib><creatorcontrib>Neuzeret, Pierre-Charles</creatorcontrib><creatorcontrib>Denoroy, Luc</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parrot, Sandrine</au><au>Neuzeret, Pierre-Charles</au><au>Denoroy, Luc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid and sensitive method for the analysis of brain monoamine neurotransmitters using ultra-fast liquid chromatography coupled to electrochemical detection</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2011-12-15</date><risdate>2011</risdate><volume>879</volume><issue>32</issue><spage>3871</spage><epage>3878</epage><pages>3871-3878</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>► Catecholamines and indolamines are the most studied monoamines in neuroscience. ► Time of routine analyses by conventional HPLC and electrochemical detection is too long. ► Use of sub-2
μm particles increases the throughput of analysis using ultra-fast HPLC. ► Brain monoamines can be determined in various tissue samples by this way. ► Potential applications of ultra-fast HPLC in brain dialysis.
Electrochemical detection is often used to detect catecholamines and indolamines in brain samples that have been separated by conventional reverse-phase high performance liquid chromatography (HPLC). This paper presents the transfer of an existing chromatographic method for the determination of monoamines in brain tissues using 5
μm granulometry HPLC columns to columns with a particle diameter less than 3
μm. Several parameters (repeatability, linearity, accuracy, limit of detection, and stability of samples) for this new ultrafast high performance liquid chromatography (UHPLC) method were examined after optimization of the analytical conditions. The separation of seven compounds, noradrenaline, dopamine and three of its metabolites, dihydroxyphenylacetic acid, homovanillic acid, and 3-methoxytyramine, and serotonin and its metabolite, 5-hydroxyindole-3-acetic acid was analyzed using this UHPLC–electrochemical detection method. The final method, which was applied to brain tissue extracts from mice, rats, and cats, decreased analysis time by a factor of 4 compared to HPLC, while guaranteeing good analytical performance.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22100552</pmid><doi>10.1016/j.jchromb.2011.10.038</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-12, Vol.879 (32), p.3871-3878 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 3,4-Dihydroxyphenylacetic Acid - analysis Analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Brain Brain Chemistry Brain samples Catecholamines - analysis Cats Chromatography Chromatography, High Pressure Liquid - methods detection limit dopamine Drug Stability Electrochemical detection electrochemistry Fundamental and applied biological sciences. Psychology General pharmacology High performance liquid chromatography homovanillic acid Homovanillic Acid - analysis Limit of Detection Linearity Liquid chromatography Mathematical analysis Medical sciences Metabolite Metabolites Mice Monoamine monoamines Neurotransmitter Agents - analysis Neurotransmitters norepinephrine Pharmacology. Drug treatments Rats Reproducibility of Results reversed-phase high performance liquid chromatography serotonin Serotonin - analysis ultra-performance liquid chromatography Ultrafast high performance liquid chromatography ultrafast liquid chromatography |
| title | A rapid and sensitive method for the analysis of brain monoamine neurotransmitters using ultra-fast liquid chromatography coupled to electrochemical detection |
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