Improvement of hind-limb paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration

Somatic (adult) stem cells are thought to have pluripotency, just as do embryotic stem (ES) cells. We investigated the possibility that grafted epithelial keratinocytes could induce spinal cord regeneration in an animal model of spinal cord injury (SCI). Normal human keratinocytes were cultured by t...

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Veröffentlicht in:Journal of artificial organs 2011-12, Vol.14 (4), p.375-380
Hauptverfasser: Inoue, Hajime, Takenaga, Mitsuko, Ohta, Yuki, Tomioka, Miyuki, Watabe, Yu-Ichi, Aihara, Masaki, Kumagai, Norio
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container_end_page 380
container_issue 4
container_start_page 375
container_title Journal of artificial organs
container_volume 14
creator Inoue, Hajime
Takenaga, Mitsuko
Ohta, Yuki
Tomioka, Miyuki
Watabe, Yu-Ichi
Aihara, Masaki
Kumagai, Norio
description Somatic (adult) stem cells are thought to have pluripotency, just as do embryotic stem (ES) cells. We investigated the possibility that grafted epithelial keratinocytes could induce spinal cord regeneration in an animal model of spinal cord injury (SCI). Normal human keratinocytes were cultured by the routine technique, and normal human dermal fibroblasts were cultured by a similar method as a control group. SCI model was prepared by dropping a 10-g weight onto the exposed spinal cord of rats from a height of 25 mm, and 8 days later, the cultured cells were grafted into the injury site. Motor function was significantly improved in the cultured-keratinocyte-grafted group compared with that in the fibroblast-grafted group. After functional observation, human nestin- and nuclei-positive cells were found at the grafted spinal cord. Grafted cultured keratinocytes induced in vitro morphological changes in the neural induction medium. These results indicated one possibility that some of the grafted cultured keratinocytes survived and could have contributed to neural regeneration. On the other hand, it should be noted that the grafted cultured keratinocytes secreted a large amount of enzymes and/or growth factors. Therefore, another possibility is that the grafted-keratinocyte-derived factors could induce survived cell growth and endogenous neural differentiation of spinal-nerve-derived stem cells surrounding the injured spinal cord, leading to functional recovery. Epithelial stem cell therapy may be applied clinically in the near future to treat SCI.
doi_str_mv 10.1007/s10047-011-0598-0
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subjects Animals
Biomedical Engineering and Bioengineering
Brief Communication
Cardiac Surgery
Cell Differentiation
Cells, Cultured
Female
Humans
Keratinocytes - cytology
Keratinocytes - transplantation
Medicine
Medicine & Public Health
Nephrology
Paralysis - etiology
Paralysis - therapy
Rats
Rats, Sprague-Dawley
Spinal Cord Injuries - complications
Spinal Cord Injuries - therapy
Spinal Cord Regeneration
title Improvement of hind-limb paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration
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