Improvement of hind-limb paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration
Somatic (adult) stem cells are thought to have pluripotency, just as do embryotic stem (ES) cells. We investigated the possibility that grafted epithelial keratinocytes could induce spinal cord regeneration in an animal model of spinal cord injury (SCI). Normal human keratinocytes were cultured by t...
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Veröffentlicht in: | Journal of artificial organs 2011-12, Vol.14 (4), p.375-380 |
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creator | Inoue, Hajime Takenaga, Mitsuko Ohta, Yuki Tomioka, Miyuki Watabe, Yu-Ichi Aihara, Masaki Kumagai, Norio |
description | Somatic (adult) stem cells are thought to have pluripotency, just as do embryotic stem (ES) cells. We investigated the possibility that grafted epithelial keratinocytes could induce spinal cord regeneration in an animal model of spinal cord injury (SCI). Normal human keratinocytes were cultured by the routine technique, and normal human dermal fibroblasts were cultured by a similar method as a control group. SCI model was prepared by dropping a 10-g weight onto the exposed spinal cord of rats from a height of 25 mm, and 8 days later, the cultured cells were grafted into the injury site. Motor function was significantly improved in the cultured-keratinocyte-grafted group compared with that in the fibroblast-grafted group. After functional observation, human nestin- and nuclei-positive cells were found at the grafted spinal cord. Grafted cultured keratinocytes induced in vitro morphological changes in the neural induction medium. These results indicated one possibility that some of the grafted cultured keratinocytes survived and could have contributed to neural regeneration. On the other hand, it should be noted that the grafted cultured keratinocytes secreted a large amount of enzymes and/or growth factors. Therefore, another possibility is that the grafted-keratinocyte-derived factors could induce survived cell growth and endogenous neural differentiation of spinal-nerve-derived stem cells surrounding the injured spinal cord, leading to functional recovery. Epithelial stem cell therapy may be applied clinically in the near future to treat SCI. |
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We investigated the possibility that grafted epithelial keratinocytes could induce spinal cord regeneration in an animal model of spinal cord injury (SCI). Normal human keratinocytes were cultured by the routine technique, and normal human dermal fibroblasts were cultured by a similar method as a control group. SCI model was prepared by dropping a 10-g weight onto the exposed spinal cord of rats from a height of 25 mm, and 8 days later, the cultured cells were grafted into the injury site. Motor function was significantly improved in the cultured-keratinocyte-grafted group compared with that in the fibroblast-grafted group. After functional observation, human nestin- and nuclei-positive cells were found at the grafted spinal cord. Grafted cultured keratinocytes induced in vitro morphological changes in the neural induction medium. These results indicated one possibility that some of the grafted cultured keratinocytes survived and could have contributed to neural regeneration. On the other hand, it should be noted that the grafted cultured keratinocytes secreted a large amount of enzymes and/or growth factors. Therefore, another possibility is that the grafted-keratinocyte-derived factors could induce survived cell growth and endogenous neural differentiation of spinal-nerve-derived stem cells surrounding the injured spinal cord, leading to functional recovery. Epithelial stem cell therapy may be applied clinically in the near future to treat SCI.</description><identifier>ISSN: 1434-7229</identifier><identifier>EISSN: 1619-0904</identifier><identifier>DOI: 10.1007/s10047-011-0598-0</identifier><identifier>PMID: 21842261</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Animals ; Biomedical Engineering and Bioengineering ; Brief Communication ; Cardiac Surgery ; Cell Differentiation ; Cells, Cultured ; Female ; Humans ; Keratinocytes - cytology ; Keratinocytes - transplantation ; Medicine ; Medicine & Public Health ; Nephrology ; Paralysis - etiology ; Paralysis - therapy ; Rats ; Rats, Sprague-Dawley ; Spinal Cord Injuries - complications ; Spinal Cord Injuries - therapy ; Spinal Cord Regeneration</subject><ispartof>Journal of artificial organs, 2011-12, Vol.14 (4), p.375-380</ispartof><rights>The Japanese Society for Artificial Organs 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-237e149f34487cf85766557d88edbebb38553c4a1558c520f9878a11da2568143</citedby><cites>FETCH-LOGICAL-c490t-237e149f34487cf85766557d88edbebb38553c4a1558c520f9878a11da2568143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10047-011-0598-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10047-011-0598-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21842261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inoue, Hajime</creatorcontrib><creatorcontrib>Takenaga, Mitsuko</creatorcontrib><creatorcontrib>Ohta, Yuki</creatorcontrib><creatorcontrib>Tomioka, Miyuki</creatorcontrib><creatorcontrib>Watabe, Yu-Ichi</creatorcontrib><creatorcontrib>Aihara, Masaki</creatorcontrib><creatorcontrib>Kumagai, Norio</creatorcontrib><title>Improvement of hind-limb paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration</title><title>Journal of artificial organs</title><addtitle>J Artif Organs</addtitle><addtitle>J Artif Organs</addtitle><description>Somatic (adult) stem cells are thought to have pluripotency, just as do embryotic stem (ES) cells. We investigated the possibility that grafted epithelial keratinocytes could induce spinal cord regeneration in an animal model of spinal cord injury (SCI). Normal human keratinocytes were cultured by the routine technique, and normal human dermal fibroblasts were cultured by a similar method as a control group. SCI model was prepared by dropping a 10-g weight onto the exposed spinal cord of rats from a height of 25 mm, and 8 days later, the cultured cells were grafted into the injury site. Motor function was significantly improved in the cultured-keratinocyte-grafted group compared with that in the fibroblast-grafted group. After functional observation, human nestin- and nuclei-positive cells were found at the grafted spinal cord. Grafted cultured keratinocytes induced in vitro morphological changes in the neural induction medium. These results indicated one possibility that some of the grafted cultured keratinocytes survived and could have contributed to neural regeneration. On the other hand, it should be noted that the grafted cultured keratinocytes secreted a large amount of enzymes and/or growth factors. Therefore, another possibility is that the grafted-keratinocyte-derived factors could induce survived cell growth and endogenous neural differentiation of spinal-nerve-derived stem cells surrounding the injured spinal cord, leading to functional recovery. Epithelial stem cell therapy may be applied clinically in the near future to treat SCI.</description><subject>Animals</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Brief Communication</subject><subject>Cardiac Surgery</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Humans</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - transplantation</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nephrology</subject><subject>Paralysis - etiology</subject><subject>Paralysis - therapy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spinal Cord Injuries - complications</subject><subject>Spinal Cord Injuries - therapy</subject><subject>Spinal Cord Regeneration</subject><issn>1434-7229</issn><issn>1619-0904</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1UU2L1TAULaI44-gPcCMBF66iuWnSJO5kcHRgwI2uQ9qmfXm2yTNpZ-hf8ld637xRRBBCbuB83HBOVb0E9hYYU-8K3kJRBkCZNJqyR9U5NGAoM0w8xreoBVWcm7PqWSl7xkBJxZ5WZxy04LyB8-rn9XzI6dbPPi4kDWQXYk-nMLfk4LKbthIKGdI0pbsQR7Jkt85uCR0phxDdRLqUexLifs0bDpLdUki7kTG7YTkKYsoz0naoiuS7RzzE1G2LL-9J9Hek89NElx0Ch40UtF_8uOHCjGi-9ST70cd7WYrPqyeDm4p_8TAvqm9XH79efqY3Xz5dX364oZ0wbKG8Vh6EGWohtOoGLVXTSKl6rX3f-rattZR1JxxIqTvJ2WC00g6gd1w2GiO7qN6cfDGYH6svi51DOX7URZ_WYg0z3DCoNTJf_8PcpzVjLsUCAMdTg0QWnFhdTqVkP9hDDrPLmwVmjz3aU48We7THHi1DzasH57Wdff9H8bs4JPAToSAUR5__Wv1f118mAatV</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Inoue, Hajime</creator><creator>Takenaga, Mitsuko</creator><creator>Ohta, Yuki</creator><creator>Tomioka, Miyuki</creator><creator>Watabe, Yu-Ichi</creator><creator>Aihara, Masaki</creator><creator>Kumagai, Norio</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20111201</creationdate><title>Improvement of hind-limb paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration</title><author>Inoue, Hajime ; Takenaga, Mitsuko ; Ohta, Yuki ; Tomioka, Miyuki ; Watabe, Yu-Ichi ; Aihara, Masaki ; Kumagai, Norio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-237e149f34487cf85766557d88edbebb38553c4a1558c520f9878a11da2568143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Brief Communication</topic><topic>Cardiac Surgery</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Humans</topic><topic>Keratinocytes - 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Academic</collection><jtitle>Journal of artificial organs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inoue, Hajime</au><au>Takenaga, Mitsuko</au><au>Ohta, Yuki</au><au>Tomioka, Miyuki</au><au>Watabe, Yu-Ichi</au><au>Aihara, Masaki</au><au>Kumagai, Norio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement of hind-limb paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration</atitle><jtitle>Journal of artificial organs</jtitle><stitle>J Artif Organs</stitle><addtitle>J Artif Organs</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>14</volume><issue>4</issue><spage>375</spage><epage>380</epage><pages>375-380</pages><issn>1434-7229</issn><eissn>1619-0904</eissn><abstract>Somatic (adult) stem cells are thought to have pluripotency, just as do embryotic stem (ES) cells. We investigated the possibility that grafted epithelial keratinocytes could induce spinal cord regeneration in an animal model of spinal cord injury (SCI). Normal human keratinocytes were cultured by the routine technique, and normal human dermal fibroblasts were cultured by a similar method as a control group. SCI model was prepared by dropping a 10-g weight onto the exposed spinal cord of rats from a height of 25 mm, and 8 days later, the cultured cells were grafted into the injury site. Motor function was significantly improved in the cultured-keratinocyte-grafted group compared with that in the fibroblast-grafted group. After functional observation, human nestin- and nuclei-positive cells were found at the grafted spinal cord. Grafted cultured keratinocytes induced in vitro morphological changes in the neural induction medium. These results indicated one possibility that some of the grafted cultured keratinocytes survived and could have contributed to neural regeneration. On the other hand, it should be noted that the grafted cultured keratinocytes secreted a large amount of enzymes and/or growth factors. Therefore, another possibility is that the grafted-keratinocyte-derived factors could induce survived cell growth and endogenous neural differentiation of spinal-nerve-derived stem cells surrounding the injured spinal cord, leading to functional recovery. Epithelial stem cell therapy may be applied clinically in the near future to treat SCI.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>21842261</pmid><doi>10.1007/s10047-011-0598-0</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Biomedical Engineering and Bioengineering Brief Communication Cardiac Surgery Cell Differentiation Cells, Cultured Female Humans Keratinocytes - cytology Keratinocytes - transplantation Medicine Medicine & Public Health Nephrology Paralysis - etiology Paralysis - therapy Rats Rats, Sprague-Dawley Spinal Cord Injuries - complications Spinal Cord Injuries - therapy Spinal Cord Regeneration |
title | Improvement of hind-limb paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration |
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