Kinetic characterization and Mg2+ enhancement of Streptomyces griseocarneus sphingomyelinase C produced by recombinant Streptomyces lividans

Sphingomyelinase C (SMC) of the actinomycete, Streptomycesgriseocarneus NBRC13471, was constitutively expressed to high levels using Streptomyces lividans host and thereafter was extracellularly secreted into the cell culture. Purified SMC had a high specific activity (approximately 550-950 U/mg) an...

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Veröffentlicht in:	Protein expression and purification 2012-02, Vol.81 (2), p.151-156
Hauptverfasser:	Sugimori, Daisuke, Matsumoto, Yusaku, Tomita, Yu, Murayama, Kazutaka, Ogino, Chiaki
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Circular Dichroism Cloning, Molecular Culture Media - metabolism Electrophoresis, Polyacrylamide Gel Enzyme Activation Enzyme Assays Genetic Vectors - genetics Genetic Vectors - metabolism Kinetics Magnesium Chloride - metabolism Magnesium Chloride - pharmacology Micelles Octoxynol - metabolism Plasmids - genetics Plasmids - metabolism Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Spectrophotometry, Atomic Sphingomyelin Phosphodiesterase - genetics Sphingomyelin Phosphodiesterase - isolation & purification Sphingomyelin Phosphodiesterase - metabolism Sphingomyelins - metabolism Streptomyces - drug effects Streptomyces - enzymology Streptomyces - genetics Substrate Specificity Transformation, Bacterial
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