Purification of bacterial genomic DNA in less than 20 min using chelex-100 microwave: examples from strains of lactic acid bacteria isolated from soil samples

We established a Chelex 100-Microwave method for the purification of bacterial genomic DNA (gDNA) in less than 20 min with high yield and good quality, useful for multiple purposes. It combines Chelex 100, proteinase K, RNase A and heating in a microwave oven. The resulting gDNA was used directly to...

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Veröffentlicht in:	Antonie van Leeuwenhoek 2010-11, Vol.98 (4), p.465-474
Hauptverfasser:	Reyes-Escogido, Lourdes, Balam-Chi, Mario, Rodríguez-Buenfil, Ingrid, Valdés, Jesús, Kameyama, Luis, Martínez-Pérez, Francisco
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Biological and medical sciences Biomedical and Life Sciences Contaminants Deoxyribonucleic acid DNA DNA, Bacterial - analysis DNA, Bacterial - isolation & purification DNA, Ribosomal - genetics Endopeptidase K Enzymes Farms Fundamental and applied biological sciences. Psychology Genome, Bacterial Genomic DNA purification Genomics Geologic Sediments - microbiology Gram-negative bacteria Lactic Acid - metabolism Lactic acid bacteria Lactobacillus Lactobacillus - classification Lactobacillus - genetics Lactobacillus - isolation & purification Lactobacillus fermentum Lactobacillus plantarum Lagoons Life Sciences Livestock Livestock farming Medical Microbiology Mexico Microbial geography Microbiology Microwave oven Microwaves Original Paper Pediococcus - classification Pediococcus - genetics Pediococcus - isolation & purification Pediococcus acidilactici Pediococcus pentosaceus phenolic compounds Phenols Plant Sciences Polymerase Chain Reaction Resins, Synthetic ribonuclease A Ribonuclease, Pancreatic RNA, Ribosomal, 16S - genetics rRNA 16S Sediments Sequence Analysis, DNA Soil Soil Microbiology Soil microorganisms Soil Science & Conservation Wetlands
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