Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.)
Multiple shoot induction in Litchi chinensis Sonn. (litchi) has been achieved by two methods: (1) direct germination of litchi seeds in 6-benzylaminopurine (20 mg l-1)-supplemented MS liquid medium and supported on a filter-paper bridge and (2) in planta treatment with 6-benzylaminopurine (100 micro...
Gespeichert in:
| Veröffentlicht in: | Plant cell reports 1999-03, Vol.18 (7/8), p.691-695 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 695 |
|---|---|
| container_issue | 7/8 |
| container_start_page | 691 |
| container_title | Plant cell reports |
| container_volume | 18 |
| creator | Das, D.K Shiva Prakash, N Bhalla-Sarin, N |
| description | Multiple shoot induction in Litchi chinensis Sonn. (litchi) has been achieved by two methods: (1) direct germination of litchi seeds in 6-benzylaminopurine (20 mg l-1)-supplemented MS liquid medium and supported on a filter-paper bridge and (2) in planta treatment with 6-benzylaminopurine (100 microgram on alternate days) of the axillary bud regions of plants germinated and maintained under sterile conditions. While the former method resulted in as many as 27.5 +/- 8.6 shoot buds from the cotyledonary node within 4 weeks, the latter yielded on average approximately 8 shoot buds from each treated node in 8 weeks. The cytokinin treatment in planta consisted of placing sterile filter paper moistened with sterile distilled water over the node and adding different concentrations of 6-benzylaminopurine. Both methods of multiple shoot induction were found to be effective for the five genotypes of litchi that were tested. The shoots elongated and rooted directly in vermiculite after a pulse treatment with IBA (25 mg/ml) for 15 min. Fungus growth which is a serious problem in litchi tissue culture, was controlled using a fungicide, Bavistin, and by eliminating organic nutrients from the growth medium. |
| doi_str_mv | 10.1007/s002990050644 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_907182312</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2433382961</sourcerecordid><originalsourceid>FETCH-LOGICAL-c378t-5d6c3598dc5dc951f0b0589bd0f8658014ff48f8d58aa2e98a5c81784e19188c3</originalsourceid><addsrcrecordid>eNp9kc9rFTEQx4Mo-Hx69OwiYvWwdSY_NpOjlPoDngjWgrclzSZtyjZ5TXYP_vemvoLowcMwMPOZL9-ZYew5wjEC6HcVgBsDoGCQ8gHboBS85yB-PGQb0Bx7rVE-Zk9qvQZoTT1s2Lcv67zE_ey7epXz0sU0rW6JOXU2Td1-tmnpir_0yRf7uxxTN8fFXcXuze6QWySfaqzdWU7p-O1T9ijYufpn93nLzj-cfj_51O--fvx88n7XO6Fp6dU0OKEMTU5NzigMcAGKzMUEgQZFzWAIkgJNiqzl3pBVjlCT9GiQyIktOzro7ku-XX1dxptYnZ-bZ5_XOhrQSFwgb-Tr_5KoBxCmxZa9_Ae8zmtJbYuRSJJqB72D-gPkSq61-DDuS7yx5eeIMN49YvzrEY1_dS9qq7NzKDa5WP8MaS416oa9OGDB5tFeloacn3FA0bRQ4qDFL8ObjxY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>884854320</pqid></control><display><type>article</type><title>Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.)</title><source>Springer Nature - Complete Springer Journals</source><creator>Das, D.K ; Shiva Prakash, N ; Bhalla-Sarin, N</creator><creatorcontrib>Das, D.K ; Shiva Prakash, N ; Bhalla-Sarin, N</creatorcontrib><description>Multiple shoot induction in Litchi chinensis Sonn. (litchi) has been achieved by two methods: (1) direct germination of litchi seeds in 6-benzylaminopurine (20 mg l-1)-supplemented MS liquid medium and supported on a filter-paper bridge and (2) in planta treatment with 6-benzylaminopurine (100 microgram on alternate days) of the axillary bud regions of plants germinated and maintained under sterile conditions. While the former method resulted in as many as 27.5 +/- 8.6 shoot buds from the cotyledonary node within 4 weeks, the latter yielded on average approximately 8 shoot buds from each treated node in 8 weeks. The cytokinin treatment in planta consisted of placing sterile filter paper moistened with sterile distilled water over the node and adding different concentrations of 6-benzylaminopurine. Both methods of multiple shoot induction were found to be effective for the five genotypes of litchi that were tested. The shoots elongated and rooted directly in vermiculite after a pulse treatment with IBA (25 mg/ml) for 15 min. Fungus growth which is a serious problem in litchi tissue culture, was controlled using a fungicide, Bavistin, and by eliminating organic nutrients from the growth medium.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s002990050644</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>6-benzylaminopurine ; application rate ; benzyladenine ; biochemical techniques ; Biological and medical sciences ; Biotechnology ; Buds ; cotyledons ; culture media ; Distilled water ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Fungicides ; genotype ; Genotypes ; growth ; In vitro propagation: entire plant regeneration from tissues and cell cultures ; indole butyric acid ; Litchi chinensis ; Methods. Procedures. Technologies ; micropropagation ; Plant cells and fungal cells ; regenerative ability ; rooting ; seed germination ; shoots</subject><ispartof>Plant cell reports, 1999-03, Vol.18 (7/8), p.691-695</ispartof><rights>1999 INIST-CNRS</rights><rights>Springer-Verlag Berlin Heidelberg 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-5d6c3598dc5dc951f0b0589bd0f8658014ff48f8d58aa2e98a5c81784e19188c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1724717$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Das, D.K</creatorcontrib><creatorcontrib>Shiva Prakash, N</creatorcontrib><creatorcontrib>Bhalla-Sarin, N</creatorcontrib><title>Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.)</title><title>Plant cell reports</title><description>Multiple shoot induction in Litchi chinensis Sonn. (litchi) has been achieved by two methods: (1) direct germination of litchi seeds in 6-benzylaminopurine (20 mg l-1)-supplemented MS liquid medium and supported on a filter-paper bridge and (2) in planta treatment with 6-benzylaminopurine (100 microgram on alternate days) of the axillary bud regions of plants germinated and maintained under sterile conditions. While the former method resulted in as many as 27.5 +/- 8.6 shoot buds from the cotyledonary node within 4 weeks, the latter yielded on average approximately 8 shoot buds from each treated node in 8 weeks. The cytokinin treatment in planta consisted of placing sterile filter paper moistened with sterile distilled water over the node and adding different concentrations of 6-benzylaminopurine. Both methods of multiple shoot induction were found to be effective for the five genotypes of litchi that were tested. The shoots elongated and rooted directly in vermiculite after a pulse treatment with IBA (25 mg/ml) for 15 min. Fungus growth which is a serious problem in litchi tissue culture, was controlled using a fungicide, Bavistin, and by eliminating organic nutrients from the growth medium.</description><subject>6-benzylaminopurine</subject><subject>application rate</subject><subject>benzyladenine</subject><subject>biochemical techniques</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Buds</subject><subject>cotyledons</subject><subject>culture media</subject><subject>Distilled water</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungicides</subject><subject>genotype</subject><subject>Genotypes</subject><subject>growth</subject><subject>In vitro propagation: entire plant regeneration from tissues and cell cultures</subject><subject>indole butyric acid</subject><subject>Litchi chinensis</subject><subject>Methods. Procedures. Technologies</subject><subject>micropropagation</subject><subject>Plant cells and fungal cells</subject><subject>regenerative ability</subject><subject>rooting</subject><subject>seed germination</subject><subject>shoots</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc9rFTEQx4Mo-Hx69OwiYvWwdSY_NpOjlPoDngjWgrclzSZtyjZ5TXYP_vemvoLowcMwMPOZL9-ZYew5wjEC6HcVgBsDoGCQ8gHboBS85yB-PGQb0Bx7rVE-Zk9qvQZoTT1s2Lcv67zE_ey7epXz0sU0rW6JOXU2Td1-tmnpir_0yRf7uxxTN8fFXcXuze6QWySfaqzdWU7p-O1T9ijYufpn93nLzj-cfj_51O--fvx88n7XO6Fp6dU0OKEMTU5NzigMcAGKzMUEgQZFzWAIkgJNiqzl3pBVjlCT9GiQyIktOzro7ku-XX1dxptYnZ-bZ5_XOhrQSFwgb-Tr_5KoBxCmxZa9_Ae8zmtJbYuRSJJqB72D-gPkSq61-DDuS7yx5eeIMN49YvzrEY1_dS9qq7NzKDa5WP8MaS416oa9OGDB5tFeloacn3FA0bRQ4qDFL8ObjxY</recordid><startdate>19990301</startdate><enddate>19990301</enddate><creator>Das, D.K</creator><creator>Shiva Prakash, N</creator><creator>Bhalla-Sarin, N</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope></search><sort><creationdate>19990301</creationdate><title>Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.)</title><author>Das, D.K ; Shiva Prakash, N ; Bhalla-Sarin, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-5d6c3598dc5dc951f0b0589bd0f8658014ff48f8d58aa2e98a5c81784e19188c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>6-benzylaminopurine</topic><topic>application rate</topic><topic>benzyladenine</topic><topic>biochemical techniques</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Buds</topic><topic>cotyledons</topic><topic>culture media</topic><topic>Distilled water</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungicides</topic><topic>genotype</topic><topic>Genotypes</topic><topic>growth</topic><topic>In vitro propagation: entire plant regeneration from tissues and cell cultures</topic><topic>indole butyric acid</topic><topic>Litchi chinensis</topic><topic>Methods. Procedures. Technologies</topic><topic>micropropagation</topic><topic>Plant cells and fungal cells</topic><topic>regenerative ability</topic><topic>rooting</topic><topic>seed germination</topic><topic>shoots</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Das, D.K</creatorcontrib><creatorcontrib>Shiva Prakash, N</creatorcontrib><creatorcontrib>Bhalla-Sarin, N</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Das, D.K</au><au>Shiva Prakash, N</au><au>Bhalla-Sarin, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.)</atitle><jtitle>Plant cell reports</jtitle><date>1999-03-01</date><risdate>1999</risdate><volume>18</volume><issue>7/8</issue><spage>691</spage><epage>695</epage><pages>691-695</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Multiple shoot induction in Litchi chinensis Sonn. (litchi) has been achieved by two methods: (1) direct germination of litchi seeds in 6-benzylaminopurine (20 mg l-1)-supplemented MS liquid medium and supported on a filter-paper bridge and (2) in planta treatment with 6-benzylaminopurine (100 microgram on alternate days) of the axillary bud regions of plants germinated and maintained under sterile conditions. While the former method resulted in as many as 27.5 +/- 8.6 shoot buds from the cotyledonary node within 4 weeks, the latter yielded on average approximately 8 shoot buds from each treated node in 8 weeks. The cytokinin treatment in planta consisted of placing sterile filter paper moistened with sterile distilled water over the node and adding different concentrations of 6-benzylaminopurine. Both methods of multiple shoot induction were found to be effective for the five genotypes of litchi that were tested. The shoots elongated and rooted directly in vermiculite after a pulse treatment with IBA (25 mg/ml) for 15 min. Fungus growth which is a serious problem in litchi tissue culture, was controlled using a fungicide, Bavistin, and by eliminating organic nutrients from the growth medium.</abstract><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s002990050644</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 1999-03, Vol.18 (7/8), p.691-695 |
| issn | 0721-7714 1432-203X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_907182312 |
| source | Springer Nature - Complete Springer Journals |
| subjects | 6-benzylaminopurine application rate benzyladenine biochemical techniques Biological and medical sciences Biotechnology Buds cotyledons culture media Distilled water Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Fungicides genotype Genotypes growth In vitro propagation: entire plant regeneration from tissues and cell cultures indole butyric acid Litchi chinensis Methods. Procedures. Technologies micropropagation Plant cells and fungal cells regenerative ability rooting seed germination shoots |
| title | Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T05%3A13%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Multiple%20shoot%20induction%20and%20plant%20regeneration%20in%20litchi%20(Litchi%20chinensis%20Sonn.)&rft.jtitle=Plant%20cell%20reports&rft.au=Das,%20D.K&rft.date=1999-03-01&rft.volume=18&rft.issue=7/8&rft.spage=691&rft.epage=695&rft.pages=691-695&rft.issn=0721-7714&rft.eissn=1432-203X&rft.coden=PCRPD8&rft_id=info:doi/10.1007/s002990050644&rft_dat=%3Cproquest_cross%3E2433382961%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=884854320&rft_id=info:pmid/&rfr_iscdi=true |