FTA Card Utility for PCR Detection of Mycobacterium leprae
The suitability of the FTA® elute card for the collection of slit skin smear (SSS) samples for PCR detection of Mycobacterium leprae was evaluated. A total of 192 SSS leprosy samples, of bacillary index (BI) 1 to 5, were collected from patients attending two skin clinics in Myanmar and preserved usi...
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| Veröffentlicht in: | Japanese Journal of Infectious Diseases 2011/05/31, Vol.64(3), pp.246-248 |
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| container_title | Japanese Journal of Infectious Diseases |
| container_volume | 64 |
| creator | Aye, Khin Saw Matsuoka, Masanori Kai, Masanori Kyaw, Kyaw Win, Aye Aye Shwe, Mu Mu Thein, Min Htoo, Maung Maung Htoon, Myo Thet |
| description | The suitability of the FTA® elute card for the collection of slit skin smear (SSS) samples for PCR detection of Mycobacterium leprae was evaluated. A total of 192 SSS leprosy samples, of bacillary index (BI) 1 to 5, were collected from patients attending two skin clinics in Myanmar and preserved using both FTA® elute cards and 70% ethanol tubes. To compare the efficacy of PCR detection of DNA from each BI class, PCR was performed to amplify an M. leprae-specific repetitive element. Of the 192 samples, 116 FTA® elute card and 112 70% ethanol samples were PCR positive for M. leprae DNA. When correlated with BI, area under the curve (AUC) values of the respective receiver-operating characteristic curves were similar for the FTA® elute card and ethanol collection methods (AUC=0.6). Taken together, our results indicate that the FTA® elute card, which enables the collection, transport, and archiving of clinical samples, is an attractive alternative to ethanol preservation for the detection of M. leprae DNA. |
| doi_str_mv | 10.7883/yoken.64.246 |
| format | Article |
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A total of 192 SSS leprosy samples, of bacillary index (BI) 1 to 5, were collected from patients attending two skin clinics in Myanmar and preserved using both FTA® elute cards and 70% ethanol tubes. To compare the efficacy of PCR detection of DNA from each BI class, PCR was performed to amplify an M. leprae-specific repetitive element. Of the 192 samples, 116 FTA® elute card and 112 70% ethanol samples were PCR positive for M. leprae DNA. When correlated with BI, area under the curve (AUC) values of the respective receiver-operating characteristic curves were similar for the FTA® elute card and ethanol collection methods (AUC=0.6). Taken together, our results indicate that the FTA® elute card, which enables the collection, transport, and archiving of clinical samples, is an attractive alternative to ethanol preservation for the detection of M. leprae DNA.</description><identifier>ISSN: 1344-6304</identifier><identifier>EISSN: 1884-2836</identifier><identifier>DOI: 10.7883/yoken.64.246</identifier><identifier>PMID: 21617312</identifier><language>eng</language><publisher>Japan: National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</publisher><subject>Bacteriological Techniques - methods ; Humans ; Leprosy - diagnosis ; Molecular Diagnostic Techniques - methods ; Mycobacterium leprae ; Mycobacterium leprae - classification ; Mycobacterium leprae - genetics ; Mycobacterium leprae - isolation & purification ; Polymerase Chain Reaction - methods ; ROC Curve ; Specimen Handling - methods</subject><ispartof>Japanese Journal of Infectious Diseases, 2011/05/31, Vol.64(3), pp.246-248</ispartof><rights>2011 Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-5d042b1f4fae222be5b07bfc909f3ad2753b92653369c6ddb245c7f22771d02a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21617312$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aye, Khin Saw</creatorcontrib><creatorcontrib>Matsuoka, Masanori</creatorcontrib><creatorcontrib>Kai, Masanori</creatorcontrib><creatorcontrib>Kyaw, Kyaw</creatorcontrib><creatorcontrib>Win, Aye Aye</creatorcontrib><creatorcontrib>Shwe, Mu Mu</creatorcontrib><creatorcontrib>Thein, Min</creatorcontrib><creatorcontrib>Htoo, Maung Maung</creatorcontrib><creatorcontrib>Htoon, Myo Thet</creatorcontrib><title>FTA Card Utility for PCR Detection of Mycobacterium leprae</title><title>Japanese Journal of Infectious Diseases</title><addtitle>Jpn J Infect Dis</addtitle><description>The suitability of the FTA® elute card for the collection of slit skin smear (SSS) samples for PCR detection of Mycobacterium leprae was evaluated. A total of 192 SSS leprosy samples, of bacillary index (BI) 1 to 5, were collected from patients attending two skin clinics in Myanmar and preserved using both FTA® elute cards and 70% ethanol tubes. To compare the efficacy of PCR detection of DNA from each BI class, PCR was performed to amplify an M. leprae-specific repetitive element. Of the 192 samples, 116 FTA® elute card and 112 70% ethanol samples were PCR positive for M. leprae DNA. When correlated with BI, area under the curve (AUC) values of the respective receiver-operating characteristic curves were similar for the FTA® elute card and ethanol collection methods (AUC=0.6). Taken together, our results indicate that the FTA® elute card, which enables the collection, transport, and archiving of clinical samples, is an attractive alternative to ethanol preservation for the detection of M. leprae DNA.</description><subject>Bacteriological Techniques - methods</subject><subject>Humans</subject><subject>Leprosy - diagnosis</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Mycobacterium leprae</subject><subject>Mycobacterium leprae - classification</subject><subject>Mycobacterium leprae - genetics</subject><subject>Mycobacterium leprae - isolation & purification</subject><subject>Polymerase Chain Reaction - methods</subject><subject>ROC Curve</subject><subject>Specimen Handling - methods</subject><issn>1344-6304</issn><issn>1884-2836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQQC0EonxtzCgbCyn-dswGhQJSEQi1s-U4ZwikTbHdof-elJYystyddE9veAidEtxXRcEul-0nzPqS9ymXO-iAFAXPacHkbnczznPJMO-hwxg_MKZCELyPepRIohihB-hqOL7OBjZU2STVTZ2WmW9D9jJ4zW4hgUt1O8tanz0tXVtalyDUi2nWwDxYOEZ73jYRTjb7CE2Gd-PBQz56vn8cXI9yJzRNuagwpyXx3FuglJYgSqxK7zTWntmKKsFKTaVgTGonq6qkXDjlKVWKVJhadoTO1955aL8WEJOZ1tFB09gZtItoNFakIEyof8lCFlpzrXRHXqxJF9oYA3gzD_XUhqUh2Kyymp-sRnLTZe3ws414UU6h2sK_HTvgZg18xGTfYAvYkGrXwJ-Nrcbaun26dxsMzNg3kXeKCQ</recordid><startdate>20110531</startdate><enddate>20110531</enddate><creator>Aye, Khin Saw</creator><creator>Matsuoka, Masanori</creator><creator>Kai, Masanori</creator><creator>Kyaw, Kyaw</creator><creator>Win, Aye Aye</creator><creator>Shwe, Mu Mu</creator><creator>Thein, Min</creator><creator>Htoo, Maung Maung</creator><creator>Htoon, Myo Thet</creator><general>National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20110531</creationdate><title>FTA Card Utility for PCR Detection of Mycobacterium leprae</title><author>Aye, Khin Saw ; Matsuoka, Masanori ; Kai, Masanori ; Kyaw, Kyaw ; Win, Aye Aye ; Shwe, Mu Mu ; Thein, Min ; Htoo, Maung Maung ; Htoon, Myo Thet</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-5d042b1f4fae222be5b07bfc909f3ad2753b92653369c6ddb245c7f22771d02a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacteriological Techniques - methods</topic><topic>Humans</topic><topic>Leprosy - diagnosis</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Mycobacterium leprae</topic><topic>Mycobacterium leprae - classification</topic><topic>Mycobacterium leprae - genetics</topic><topic>Mycobacterium leprae - isolation & purification</topic><topic>Polymerase Chain Reaction - methods</topic><topic>ROC Curve</topic><topic>Specimen Handling - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aye, Khin Saw</creatorcontrib><creatorcontrib>Matsuoka, Masanori</creatorcontrib><creatorcontrib>Kai, Masanori</creatorcontrib><creatorcontrib>Kyaw, Kyaw</creatorcontrib><creatorcontrib>Win, Aye Aye</creatorcontrib><creatorcontrib>Shwe, Mu Mu</creatorcontrib><creatorcontrib>Thein, Min</creatorcontrib><creatorcontrib>Htoo, Maung Maung</creatorcontrib><creatorcontrib>Htoon, Myo Thet</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Japanese Journal of Infectious Diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aye, Khin Saw</au><au>Matsuoka, Masanori</au><au>Kai, Masanori</au><au>Kyaw, Kyaw</au><au>Win, Aye Aye</au><au>Shwe, Mu Mu</au><au>Thein, Min</au><au>Htoo, Maung Maung</au><au>Htoon, Myo Thet</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FTA Card Utility for PCR Detection of Mycobacterium leprae</atitle><jtitle>Japanese Journal of Infectious Diseases</jtitle><addtitle>Jpn J Infect Dis</addtitle><date>2011-05-31</date><risdate>2011</risdate><volume>64</volume><issue>3</issue><spage>246</spage><epage>248</epage><pages>246-248</pages><artnum>64.246</artnum><issn>1344-6304</issn><eissn>1884-2836</eissn><abstract>The suitability of the FTA® elute card for the collection of slit skin smear (SSS) samples for PCR detection of Mycobacterium leprae was evaluated. A total of 192 SSS leprosy samples, of bacillary index (BI) 1 to 5, were collected from patients attending two skin clinics in Myanmar and preserved using both FTA® elute cards and 70% ethanol tubes. To compare the efficacy of PCR detection of DNA from each BI class, PCR was performed to amplify an M. leprae-specific repetitive element. Of the 192 samples, 116 FTA® elute card and 112 70% ethanol samples were PCR positive for M. leprae DNA. When correlated with BI, area under the curve (AUC) values of the respective receiver-operating characteristic curves were similar for the FTA® elute card and ethanol collection methods (AUC=0.6). Taken together, our results indicate that the FTA® elute card, which enables the collection, transport, and archiving of clinical samples, is an attractive alternative to ethanol preservation for the detection of M. leprae DNA.</abstract><cop>Japan</cop><pub>National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</pub><pmid>21617312</pmid><doi>10.7883/yoken.64.246</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Bacteriological Techniques - methods Humans Leprosy - diagnosis Molecular Diagnostic Techniques - methods Mycobacterium leprae Mycobacterium leprae - classification Mycobacterium leprae - genetics Mycobacterium leprae - isolation & purification Polymerase Chain Reaction - methods ROC Curve Specimen Handling - methods |
| title | FTA Card Utility for PCR Detection of Mycobacterium leprae |
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