Influence of Bordetella pertussis operon bvgAS on formation and resolution of plasmid-chromosome cointegrates in Escherichia coli K12 mutants with mutational changes on common components of phosphoenolpyruvate-sugar-phosphotransferase system
Several plasmids that contain genetically marked variants of the TnBP transposon isolated from Bordetella pertussis have been constructed based on a thermosensitive plasmid. The frequencies of the integration of these plasmids into an E. coli K12 chromosome at a nonpermissive temperature (42°C) have...
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| Veröffentlicht in: | Molecular genetics, microbiology and virology microbiology and virology, 2010-03, Vol.25 (1), p.8-13 |
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| container_title | Molecular genetics, microbiology and virology |
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| creator | Umyarov, A. M. Sinyashina, L. N. Amelina, I. P. Datsenko, K. A. Bol’shakova, T. N. Dobrynina, O. Yu Karataev, G. I. |
| description | Several plasmids that contain genetically marked variants of the TnBP transposon isolated from
Bordetella pertussis
have been constructed based on a thermosensitive plasmid. The frequencies of the integration of these plasmids into an
E. coli
K12 chromosome at a nonpermissive temperature (42°C) have been studied. A decline in the frequency of RSBP-induced and HPr- or enzyme I-deficient cointegrate formation between the plasmid and the K12 bacterial chromosome were detected. The introduction of the
bvgAS
operon of
B. pertussis
at the trans-position into the mutant
E. coli
K12 bacteria restores the frequency of the formation and resolution of RSBP-induced cointegrates. |
| doi_str_mv | 10.3103/S0891416810010027 |
| format | Article |
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Bordetella pertussis
have been constructed based on a thermosensitive plasmid. The frequencies of the integration of these plasmids into an
E. coli
K12 chromosome at a nonpermissive temperature (42°C) have been studied. A decline in the frequency of RSBP-induced and HPr- or enzyme I-deficient cointegrate formation between the plasmid and the K12 bacterial chromosome were detected. The introduction of the
bvgAS
operon of
B. pertussis
at the trans-position into the mutant
E. coli
K12 bacteria restores the frequency of the formation and resolution of RSBP-induced cointegrates.</description><identifier>ISSN: 0891-4168</identifier><identifier>EISSN: 1934-841X</identifier><identifier>DOI: 10.3103/S0891416810010027</identifier><language>eng</language><publisher>Heidelberg: Allerton Press, Inc</publisher><subject>Biomedical and Life Sciences ; Bordetella pertussis ; Chromosomes ; Experimental Works ; Life Sciences ; Microbiology ; Molecular Medicine</subject><ispartof>Molecular genetics, microbiology and virology, 2010-03, Vol.25 (1), p.8-13</ispartof><rights>Allerton Press, Inc. 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c320t-12965dd335cccd74ab0fae83998a20261eecc376598a65866d1f534b15e24e783</citedby><cites>FETCH-LOGICAL-c320t-12965dd335cccd74ab0fae83998a20261eecc376598a65866d1f534b15e24e783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.3103/S0891416810010027$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.3103/S0891416810010027$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Umyarov, A. M.</creatorcontrib><creatorcontrib>Sinyashina, L. N.</creatorcontrib><creatorcontrib>Amelina, I. P.</creatorcontrib><creatorcontrib>Datsenko, K. A.</creatorcontrib><creatorcontrib>Bol’shakova, T. N.</creatorcontrib><creatorcontrib>Dobrynina, O. Yu</creatorcontrib><creatorcontrib>Karataev, G. I.</creatorcontrib><title>Influence of Bordetella pertussis operon bvgAS on formation and resolution of plasmid-chromosome cointegrates in Escherichia coli K12 mutants with mutational changes on common components of phosphoenolpyruvate-sugar-phosphotransferase system</title><title>Molecular genetics, microbiology and virology</title><addtitle>Mol. Genet. Microbiol. Virol</addtitle><description>Several plasmids that contain genetically marked variants of the TnBP transposon isolated from
Bordetella pertussis
have been constructed based on a thermosensitive plasmid. The frequencies of the integration of these plasmids into an
E. coli
K12 chromosome at a nonpermissive temperature (42°C) have been studied. A decline in the frequency of RSBP-induced and HPr- or enzyme I-deficient cointegrate formation between the plasmid and the K12 bacterial chromosome were detected. The introduction of the
bvgAS
operon of
B. pertussis
at the trans-position into the mutant
E. coli
K12 bacteria restores the frequency of the formation and resolution of RSBP-induced cointegrates.</description><subject>Biomedical and Life Sciences</subject><subject>Bordetella pertussis</subject><subject>Chromosomes</subject><subject>Experimental Works</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Molecular Medicine</subject><issn>0891-4168</issn><issn>1934-841X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9Uctu1TAQjRBIXEo_gJ13rAJ-JE6yLFWBikosWqTuIl9ncuPKj-Cxi-5n9w9wertDQrI1Z3QeI81U1QdGPwlGxedb2g-sYbJnlJbHu1fVjg2iqfuG3b-udhtdb_zb6h3iA6Wt5ELuqqdrP9sMXgMJM_kS4gQJrFVkhZgyokESCgye7B8PF7ekgDlEp5IpSPmJRMBg83NbAlar0Jmp1ksMLmBwQHQwPsEhqgRIjCdXqBeIRi9GFc4a8oNx4nJSPiH5Y9Ly3GyByhK9KH8ovpKug3OnsgYPm3ibtwQsH3yw6zHmxzKkxnxQsX5hUlQeZ4gKgeARE7j31ZtZWYTzl3pW_fp6dXf5vb75-e368uKm1oLTVDM-yHaahGi11lPXqD2dFfRiGHrFKZcMQGvRybb0su2lnNjcimbPWuANdL04qz6ectcYfmfANDqDetuth5BxHGjHuraRrCjZSaljQIwwj2s0TsXjyOi4XXf857rFw08eLNqyojg-hBzLyvA_pr_g2K98</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Umyarov, A. M.</creator><creator>Sinyashina, L. N.</creator><creator>Amelina, I. P.</creator><creator>Datsenko, K. A.</creator><creator>Bol’shakova, T. N.</creator><creator>Dobrynina, O. Yu</creator><creator>Karataev, G. I.</creator><general>Allerton Press, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20100301</creationdate><title>Influence of Bordetella pertussis operon bvgAS on formation and resolution of plasmid-chromosome cointegrates in Escherichia coli K12 mutants with mutational changes on common components of phosphoenolpyruvate-sugar-phosphotransferase system</title><author>Umyarov, A. M. ; Sinyashina, L. N. ; Amelina, I. P. ; Datsenko, K. A. ; Bol’shakova, T. N. ; Dobrynina, O. Yu ; Karataev, G. I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-12965dd335cccd74ab0fae83998a20261eecc376598a65866d1f534b15e24e783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biomedical and Life Sciences</topic><topic>Bordetella pertussis</topic><topic>Chromosomes</topic><topic>Experimental Works</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Molecular Medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Umyarov, A. M.</creatorcontrib><creatorcontrib>Sinyashina, L. N.</creatorcontrib><creatorcontrib>Amelina, I. P.</creatorcontrib><creatorcontrib>Datsenko, K. A.</creatorcontrib><creatorcontrib>Bol’shakova, T. N.</creatorcontrib><creatorcontrib>Dobrynina, O. Yu</creatorcontrib><creatorcontrib>Karataev, G. I.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular genetics, microbiology and virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Umyarov, A. M.</au><au>Sinyashina, L. N.</au><au>Amelina, I. P.</au><au>Datsenko, K. A.</au><au>Bol’shakova, T. N.</au><au>Dobrynina, O. Yu</au><au>Karataev, G. I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of Bordetella pertussis operon bvgAS on formation and resolution of plasmid-chromosome cointegrates in Escherichia coli K12 mutants with mutational changes on common components of phosphoenolpyruvate-sugar-phosphotransferase system</atitle><jtitle>Molecular genetics, microbiology and virology</jtitle><stitle>Mol. Genet. Microbiol. Virol</stitle><date>2010-03-01</date><risdate>2010</risdate><volume>25</volume><issue>1</issue><spage>8</spage><epage>13</epage><pages>8-13</pages><issn>0891-4168</issn><eissn>1934-841X</eissn><abstract>Several plasmids that contain genetically marked variants of the TnBP transposon isolated from
Bordetella pertussis
have been constructed based on a thermosensitive plasmid. The frequencies of the integration of these plasmids into an
E. coli
K12 chromosome at a nonpermissive temperature (42°C) have been studied. A decline in the frequency of RSBP-induced and HPr- or enzyme I-deficient cointegrate formation between the plasmid and the K12 bacterial chromosome were detected. The introduction of the
bvgAS
operon of
B. pertussis
at the trans-position into the mutant
E. coli
K12 bacteria restores the frequency of the formation and resolution of RSBP-induced cointegrates.</abstract><cop>Heidelberg</cop><pub>Allerton Press, Inc</pub><doi>10.3103/S0891416810010027</doi><tpages>6</tpages></addata></record> |
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| ispartof | Molecular genetics, microbiology and virology, 2010-03, Vol.25 (1), p.8-13 |
| issn | 0891-4168 1934-841X |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Biomedical and Life Sciences Bordetella pertussis Chromosomes Experimental Works Life Sciences Microbiology Molecular Medicine |
| title | Influence of Bordetella pertussis operon bvgAS on formation and resolution of plasmid-chromosome cointegrates in Escherichia coli K12 mutants with mutational changes on common components of phosphoenolpyruvate-sugar-phosphotransferase system |
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