Expressed protein ligation for a large dimeric protein

Expressed protein ligation (EPL) is a protein engineering tool for post-translational ligation of protein or peptide fragments. This technique allows modification of specific parts of proteins, opening possibilities for incorporating probes for biophysical applications such as nuclear magnetic reson...

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Veröffentlicht in:	Protein engineering, design and selection design and selection, 2011-06, Vol.24 (6), p.495-501
Hauptverfasser:	Karagöz, G. Elif, Sinnige, Tessa, Hsieh, Ofey, Rüdiger, Stefan G.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chaperones Chromatography Electrophoresis, Polyacrylamide Gel fluorescence spectroscopy Fluorescent indicators HSP90 Heat-Shock Proteins - chemistry HSP90 Heat-Shock Proteins - metabolism Hsp90 protein Humans Hydrogen-Ion Concentration Inteins Isotopes Models, Molecular Molecular modelling N-Terminus N.M.R Post-translation Protein Engineering - methods Protein Folding Protein Interaction Domains and Motifs Protein Multimerization
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