Mast cell chymase is present in uterine cervical carcinoma and it detaches viable and growing cervical squamous carcinoma cells from substratum in vitro
Increased numbers of mast cells is a typical feature of a variety of human cancers. The major mediators in the secretory granules of the MC TC type of mast cells, serine proteinases tryptase and chymase, may be involved in squamous cell carcinoma (SCC) lesions by inducing matrix remodeling and epith...
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| Veröffentlicht in: | Archives of Dermatological Research 2011-09, Vol.303 (7), p.499-512 |
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| creator | Diaconu, Nicolae-Costin Rummukainen, Jaana Naukkarinen, Anita Mättö, Mikko Harvima, Rauno J. Pelkonen, Jukka Harvima, Ilkka T. |
| description | Increased numbers of mast cells is a typical feature of a variety of human cancers. The major mediators in the secretory granules of the MC
TC
type of mast cells, serine proteinases tryptase and chymase, may be involved in squamous cell carcinoma (SCC) lesions by inducing matrix remodeling and epithelial cell detachment. The objective of this study was to analyze immunohistochemically whether MC
TC
mast cells as well as protease inhibitors, squamous cell carcinoma antigens (SCCAs), are present in the uterine cervical SCC. In addition, the effect of tryptase and chymase on uterine cervical SCC cell lines was studied in vitro. Here we report that tryptase- and chymase-positive mast cells are present in significant numbers in the peritumoral stroma of SCC lesions. Also, weak SCCA-2 immunoreactivity is observed in the SCC lesions, but only SCCA-1 in uterine cervical specimens with nonspecific inflammation. In cell cultures, especially chymase, but not tryptase, was shown to induce effective detachment of viable, growing and non-apoptotic SiHa SCC cells from substratum. Chymase also detached viable ME-180 SCC cells from substratum as well as degraded fibronectin. In contrast, normal keratinocytes underwent apoptotic cell death after similar prolonged chymase treatment. No inhibition of chymase was detected by SiHa cell sonicates nor did these cells express marked SCCA immunopositivity. MC
TC
mast cells containing tryptase and chymase are present in the peritumoral stroma of uterine cervical SCC and the malignant cells are only weakly immunoreactive for the chymase inhibitor SCCA-2. It is chymase that appears to be capable of inducing effective detachment of viable and growing SCC cells and therefore, it may release SCC cells from a tumor leading to spreading of malignant cells. |
| doi_str_mv | 10.1007/s00403-011-1121-4 |
| format | Article |
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TC
type of mast cells, serine proteinases tryptase and chymase, may be involved in squamous cell carcinoma (SCC) lesions by inducing matrix remodeling and epithelial cell detachment. The objective of this study was to analyze immunohistochemically whether MC
TC
mast cells as well as protease inhibitors, squamous cell carcinoma antigens (SCCAs), are present in the uterine cervical SCC. In addition, the effect of tryptase and chymase on uterine cervical SCC cell lines was studied in vitro. Here we report that tryptase- and chymase-positive mast cells are present in significant numbers in the peritumoral stroma of SCC lesions. Also, weak SCCA-2 immunoreactivity is observed in the SCC lesions, but only SCCA-1 in uterine cervical specimens with nonspecific inflammation. In cell cultures, especially chymase, but not tryptase, was shown to induce effective detachment of viable, growing and non-apoptotic SiHa SCC cells from substratum. Chymase also detached viable ME-180 SCC cells from substratum as well as degraded fibronectin. In contrast, normal keratinocytes underwent apoptotic cell death after similar prolonged chymase treatment. No inhibition of chymase was detected by SiHa cell sonicates nor did these cells express marked SCCA immunopositivity. MC
TC
mast cells containing tryptase and chymase are present in the peritumoral stroma of uterine cervical SCC and the malignant cells are only weakly immunoreactive for the chymase inhibitor SCCA-2. It is chymase that appears to be capable of inducing effective detachment of viable and growing SCC cells and therefore, it may release SCC cells from a tumor leading to spreading of malignant cells.</description><identifier>ISSN: 0340-3696</identifier><identifier>EISSN: 1432-069X</identifier><identifier>DOI: 10.1007/s00403-011-1121-4</identifier><identifier>PMID: 21274549</identifier><identifier>CODEN: ADREDL</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Antigens, Neoplasm - genetics ; Antigens, Neoplasm - metabolism ; Apoptosis - drug effects ; Biological and medical sciences ; Carcinoma - genetics ; Carcinoma - metabolism ; Carcinoma - pathology ; Cell Adhesion - drug effects ; Cell Growth Processes - drug effects ; Cell Line, Tumor ; Cell Survival - drug effects ; Chymases - genetics ; Chymases - metabolism ; Dermatology ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Epithelial Cells - pathology ; Female ; Female genital diseases ; Gene Expression Regulation, Neoplastic ; Gynecology. Andrology. Obstetrics ; Humans ; Immunohistochemistry ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Keratinocytes - pathology ; Mast Cells - drug effects ; Mast Cells - metabolism ; Mast Cells - pathology ; Medical sciences ; Medicine ; Medicine & Public Health ; Neoplasm Metastasis ; Original Paper ; Serpins - genetics ; Serpins - metabolism ; Tumors ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - metabolism ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Archives of Dermatological Research, 2011-09, Vol.303 (7), p.499-512</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-599e1c2d6cee11f220f988f528a155375078dd89becb666859cd10132b182bf3</citedby><cites>FETCH-LOGICAL-c432t-599e1c2d6cee11f220f988f528a155375078dd89becb666859cd10132b182bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00403-011-1121-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00403-011-1121-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24475752$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21274549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Diaconu, Nicolae-Costin</creatorcontrib><creatorcontrib>Rummukainen, Jaana</creatorcontrib><creatorcontrib>Naukkarinen, Anita</creatorcontrib><creatorcontrib>Mättö, Mikko</creatorcontrib><creatorcontrib>Harvima, Rauno J.</creatorcontrib><creatorcontrib>Pelkonen, Jukka</creatorcontrib><creatorcontrib>Harvima, Ilkka T.</creatorcontrib><title>Mast cell chymase is present in uterine cervical carcinoma and it detaches viable and growing cervical squamous carcinoma cells from substratum in vitro</title><title>Archives of Dermatological Research</title><addtitle>Arch Dermatol Res</addtitle><addtitle>Arch Dermatol Res</addtitle><description>Increased numbers of mast cells is a typical feature of a variety of human cancers. The major mediators in the secretory granules of the MC
TC
type of mast cells, serine proteinases tryptase and chymase, may be involved in squamous cell carcinoma (SCC) lesions by inducing matrix remodeling and epithelial cell detachment. The objective of this study was to analyze immunohistochemically whether MC
TC
mast cells as well as protease inhibitors, squamous cell carcinoma antigens (SCCAs), are present in the uterine cervical SCC. In addition, the effect of tryptase and chymase on uterine cervical SCC cell lines was studied in vitro. Here we report that tryptase- and chymase-positive mast cells are present in significant numbers in the peritumoral stroma of SCC lesions. Also, weak SCCA-2 immunoreactivity is observed in the SCC lesions, but only SCCA-1 in uterine cervical specimens with nonspecific inflammation. In cell cultures, especially chymase, but not tryptase, was shown to induce effective detachment of viable, growing and non-apoptotic SiHa SCC cells from substratum. Chymase also detached viable ME-180 SCC cells from substratum as well as degraded fibronectin. In contrast, normal keratinocytes underwent apoptotic cell death after similar prolonged chymase treatment. No inhibition of chymase was detected by SiHa cell sonicates nor did these cells express marked SCCA immunopositivity. MC
TC
mast cells containing tryptase and chymase are present in the peritumoral stroma of uterine cervical SCC and the malignant cells are only weakly immunoreactive for the chymase inhibitor SCCA-2. It is chymase that appears to be capable of inducing effective detachment of viable and growing SCC cells and therefore, it may release SCC cells from a tumor leading to spreading of malignant cells.</description><subject>Antigens, Neoplasm - genetics</subject><subject>Antigens, Neoplasm - metabolism</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Carcinoma - genetics</subject><subject>Carcinoma - metabolism</subject><subject>Carcinoma - pathology</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Growth Processes - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Chymases - genetics</subject><subject>Chymases - metabolism</subject><subject>Dermatology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - pathology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Keratinocytes - pathology</subject><subject>Mast Cells - drug effects</subject><subject>Mast Cells - metabolism</subject><subject>Mast Cells - pathology</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neoplasm Metastasis</subject><subject>Original Paper</subject><subject>Serpins - genetics</subject><subject>Serpins - metabolism</subject><subject>Tumors</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Uterine Cervical Neoplasms - metabolism</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>0340-3696</issn><issn>1432-069X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc9q3DAQxkVpaJYkD9BLEYXSkxuNJFvSsYT-g5RccujNyLK8UVjLG428IW_Sx63c3XZLoVQXgeb3zTejj5CXwN4BY-oSGZNMVAygAuBQyWdkBVLwijXm23OyYkKySjSmOSUXiPesHMUkZ-oFOeXAlaylWZHvXy1m6vxmQ93d02jR04B0mzz6mGmIdM4-hegLknbB2YLZ5EKcRktt7GnItPfZujuPdBdst_E_n9dpegxxfVThw2zHacY_5Isp0iFNI8W5w5xsnsfFchdyms7JyWA36C8O9xm5_fjh9upzdX3z6cvV--vKlV1zVRvjwfG-cd4DDJyzwWg91FxbqGuhaqZ032vTedc1TaNr43pgIHgHmneDOCNv9223aXqYPeZ2DLhMZqMv47aGKVCSGfFfUmuppTRgCvn6L_J-mlMsWyyQ0MBEUyDYQy5NiMkP7TaF0aanFli7BNzuA25LwO0ScCuL5tWh8dyNvv-t-BVnAd4cAIvl14dkowt45KRUtap54fiew1KKa5-OE_7b_Qc7Eb6Y</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Diaconu, Nicolae-Costin</creator><creator>Rummukainen, Jaana</creator><creator>Naukkarinen, Anita</creator><creator>Mättö, Mikko</creator><creator>Harvima, Rauno J.</creator><creator>Pelkonen, Jukka</creator><creator>Harvima, Ilkka T.</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20110901</creationdate><title>Mast cell chymase is present in uterine cervical carcinoma and it detaches viable and growing cervical squamous carcinoma cells from substratum in vitro</title><author>Diaconu, Nicolae-Costin ; Rummukainen, Jaana ; Naukkarinen, Anita ; Mättö, Mikko ; Harvima, Rauno J. ; Pelkonen, Jukka ; Harvima, Ilkka T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-599e1c2d6cee11f220f988f528a155375078dd89becb666859cd10132b182bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antigens, Neoplasm - genetics</topic><topic>Antigens, Neoplasm - metabolism</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Carcinoma - genetics</topic><topic>Carcinoma - metabolism</topic><topic>Carcinoma - pathology</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Growth Processes - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Chymases - genetics</topic><topic>Chymases - metabolism</topic><topic>Dermatology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - pathology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Keratinocytes - pathology</topic><topic>Mast Cells - drug effects</topic><topic>Mast Cells - metabolism</topic><topic>Mast Cells - pathology</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neoplasm Metastasis</topic><topic>Original Paper</topic><topic>Serpins - genetics</topic><topic>Serpins - metabolism</topic><topic>Tumors</topic><topic>Uterine Cervical Neoplasms - genetics</topic><topic>Uterine Cervical Neoplasms - metabolism</topic><topic>Uterine Cervical Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Diaconu, Nicolae-Costin</creatorcontrib><creatorcontrib>Rummukainen, Jaana</creatorcontrib><creatorcontrib>Naukkarinen, Anita</creatorcontrib><creatorcontrib>Mättö, Mikko</creatorcontrib><creatorcontrib>Harvima, Rauno J.</creatorcontrib><creatorcontrib>Pelkonen, Jukka</creatorcontrib><creatorcontrib>Harvima, Ilkka T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of Dermatological Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diaconu, Nicolae-Costin</au><au>Rummukainen, Jaana</au><au>Naukkarinen, Anita</au><au>Mättö, Mikko</au><au>Harvima, Rauno J.</au><au>Pelkonen, Jukka</au><au>Harvima, Ilkka T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mast cell chymase is present in uterine cervical carcinoma and it detaches viable and growing cervical squamous carcinoma cells from substratum in vitro</atitle><jtitle>Archives of Dermatological Research</jtitle><stitle>Arch Dermatol Res</stitle><addtitle>Arch Dermatol Res</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>303</volume><issue>7</issue><spage>499</spage><epage>512</epage><pages>499-512</pages><issn>0340-3696</issn><eissn>1432-069X</eissn><coden>ADREDL</coden><abstract>Increased numbers of mast cells is a typical feature of a variety of human cancers. The major mediators in the secretory granules of the MC
TC
type of mast cells, serine proteinases tryptase and chymase, may be involved in squamous cell carcinoma (SCC) lesions by inducing matrix remodeling and epithelial cell detachment. The objective of this study was to analyze immunohistochemically whether MC
TC
mast cells as well as protease inhibitors, squamous cell carcinoma antigens (SCCAs), are present in the uterine cervical SCC. In addition, the effect of tryptase and chymase on uterine cervical SCC cell lines was studied in vitro. Here we report that tryptase- and chymase-positive mast cells are present in significant numbers in the peritumoral stroma of SCC lesions. Also, weak SCCA-2 immunoreactivity is observed in the SCC lesions, but only SCCA-1 in uterine cervical specimens with nonspecific inflammation. In cell cultures, especially chymase, but not tryptase, was shown to induce effective detachment of viable, growing and non-apoptotic SiHa SCC cells from substratum. Chymase also detached viable ME-180 SCC cells from substratum as well as degraded fibronectin. In contrast, normal keratinocytes underwent apoptotic cell death after similar prolonged chymase treatment. No inhibition of chymase was detected by SiHa cell sonicates nor did these cells express marked SCCA immunopositivity. MC
TC
mast cells containing tryptase and chymase are present in the peritumoral stroma of uterine cervical SCC and the malignant cells are only weakly immunoreactive for the chymase inhibitor SCCA-2. It is chymase that appears to be capable of inducing effective detachment of viable and growing SCC cells and therefore, it may release SCC cells from a tumor leading to spreading of malignant cells.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21274549</pmid><doi>10.1007/s00403-011-1121-4</doi><tpages>14</tpages></addata></record> |
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| subjects | Antigens, Neoplasm - genetics Antigens, Neoplasm - metabolism Apoptosis - drug effects Biological and medical sciences Carcinoma - genetics Carcinoma - metabolism Carcinoma - pathology Cell Adhesion - drug effects Cell Growth Processes - drug effects Cell Line, Tumor Cell Survival - drug effects Chymases - genetics Chymases - metabolism Dermatology Epithelial Cells - drug effects Epithelial Cells - metabolism Epithelial Cells - pathology Female Female genital diseases Gene Expression Regulation, Neoplastic Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Keratinocytes - drug effects Keratinocytes - metabolism Keratinocytes - pathology Mast Cells - drug effects Mast Cells - metabolism Mast Cells - pathology Medical sciences Medicine Medicine & Public Health Neoplasm Metastasis Original Paper Serpins - genetics Serpins - metabolism Tumors Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - metabolism Uterine Cervical Neoplasms - pathology |
| title | Mast cell chymase is present in uterine cervical carcinoma and it detaches viable and growing cervical squamous carcinoma cells from substratum in vitro |
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