Coherent phenomena of charge separation in reaction centers of LL131H and LL131H/LM160H/FM197H mutants of Rhodobacter sphaeroides
Primary stage of charge separation and transfer of charges was studied in reaction centers (RCs) of point mutants LL131H and LL131H/LM160H/FM197H of the purple bacterium Rhodobacter sphaeroides by differential absorption spectroscopy with temporal resolution of 18 fsec at 90 K. Difference absorption...
Gespeichert in:
| Veröffentlicht in: | Biochemistry (Moscow) 2011-10, Vol.76 (10), p.1107-1119 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1119 |
|---|---|
| container_issue | 10 |
| container_start_page | 1107 |
| container_title | Biochemistry (Moscow) |
| container_volume | 76 |
| creator | Yakovlev, A. G. Vasilieva, L. G. Shkuropatov, A. Y. Shuvalov, V. A. |
| description | Primary stage of charge separation and transfer of charges was studied in reaction centers (RCs) of point mutants LL131H and LL131H/LM160H/FM197H of the purple bacterium
Rhodobacter sphaeroides
by differential absorption spectroscopy with temporal resolution of 18 fsec at 90 K. Difference absorption spectra measured at 0–4 psec delays after excitation of dimer P at 870 nm with 30 fsec step were obtained in the spectral range of 935–1060 nm. It was found that a decay of P* due to charge separation is considerably slower in the mutant RCs in comparison with native RCs of
Rba. sphaeroides
. Coherent oscillations were found in the kinetics of stimulated emission of the P* state at 940 nm. Fourier analysis of the oscillations revealed a set of characteristic bands in the frequency range of 20–500 cm
−1
. The most intense band has the frequency of −30 cm
−1
in RCs of mutant LL131H and in native RCs and the frequency of ∼100 cm
−1
in RCs of the triple mutant. It was found that an absorption band of bacteriochlorophyll anion B
A
−
which is registered in the difference absorption spectra of native RCs at 1020 nm is absent in the analogous spectra of the mutants. The results are analyzed in terms of the participation of the B
A
molecule in the primary electron transfer in the presence of a nuclear wave packet moving along the inharmonic surface of P* potential energy. |
| doi_str_mv | 10.1134/S000629791110004X |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_907173511</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A345694855</galeid><sourcerecordid>A345694855</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-27d6b6d530fc767ad2879d52c0437c64b53664ca3c0184cccac85633617ef2a93</originalsourceid><addsrcrecordid>eNqFkk1v1DAQhi0EotvCD-CCLDj0lK6_HR-rFWWRUiHxIXGLvM5kk2pjBzs5cOSf43QXEF9CPszY87yvZqxB6BklV5RysX5PCFHMaEMpzan49ACtqCJlwYkgD9FqKRdL_Qydp3SXr4wY_hidsRxLxtUKfd2EDiL4CY8d-DCAtzi02HU27gEnGG20Ux887j2OYN197jIPMS1gVVFOt9j65pSuq9vcwnZ9c0uN3uJhnqyf7tF3XWjCLltAxGnsLMTQN5CeoEetPSR4eooX6OPNqw-bbVG9ff1mc10VTigzFUw3aqcayUnrtNK2YaU2jWSOCK6dEjvJlRLOckdoKZxz1pVSca6ohpZZwy_Q5dF3jOHzDGmqhz45OByshzCn2hBNNZf5Y_9PSqUllTqTL34j78IcfR6jLo3mTJSKZejlEdrbA9S9b8MUrVss62supDKilDJTV3-h8mlg6F3w0Pb5_RcBPQpcDClFaOsx9oONX2pK6mU96j_WI2uen_qddwM0PxTf9yED7AikXPJ7iD8H-rfrN2odv2s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>897324862</pqid></control><display><type>article</type><title>Coherent phenomena of charge separation in reaction centers of LL131H and LL131H/LM160H/FM197H mutants of Rhodobacter sphaeroides</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Yakovlev, A. G. ; Vasilieva, L. G. ; Shkuropatov, A. Y. ; Shuvalov, V. A.</creator><creatorcontrib>Yakovlev, A. G. ; Vasilieva, L. G. ; Shkuropatov, A. Y. ; Shuvalov, V. A.</creatorcontrib><description>Primary stage of charge separation and transfer of charges was studied in reaction centers (RCs) of point mutants LL131H and LL131H/LM160H/FM197H of the purple bacterium
Rhodobacter sphaeroides
by differential absorption spectroscopy with temporal resolution of 18 fsec at 90 K. Difference absorption spectra measured at 0–4 psec delays after excitation of dimer P at 870 nm with 30 fsec step were obtained in the spectral range of 935–1060 nm. It was found that a decay of P* due to charge separation is considerably slower in the mutant RCs in comparison with native RCs of
Rba. sphaeroides
. Coherent oscillations were found in the kinetics of stimulated emission of the P* state at 940 nm. Fourier analysis of the oscillations revealed a set of characteristic bands in the frequency range of 20–500 cm
−1
. The most intense band has the frequency of −30 cm
−1
in RCs of mutant LL131H and in native RCs and the frequency of ∼100 cm
−1
in RCs of the triple mutant. It was found that an absorption band of bacteriochlorophyll anion B
A
−
which is registered in the difference absorption spectra of native RCs at 1020 nm is absent in the analogous spectra of the mutants. The results are analyzed in terms of the participation of the B
A
molecule in the primary electron transfer in the presence of a nuclear wave packet moving along the inharmonic surface of P* potential energy.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1134/S000629791110004X</identifier><identifier>PMID: 22098236</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Absorption spectroscopy ; Bacteria ; Bacteriochlorophylls - chemistry ; Bacteriochlorophylls - metabolism ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Electron transfer reactions ; Electron Transport ; Fourier analysis ; Kinetics ; Life Sciences ; Microbiology ; Mutants ; Mutation ; Photosynthesis ; Photosynthetic Reaction Center Complex Proteins - chemistry ; Photosynthetic Reaction Center Complex Proteins - genetics ; Photosynthetic Reaction Center Complex Proteins - metabolism ; Phytochemistry ; Potential energy ; Rhodobacter sphaeroides ; Rhodobacter sphaeroides - enzymology ; X-Ray Absorption Spectroscopy</subject><ispartof>Biochemistry (Moscow), 2011-10, Vol.76 (10), p.1107-1119</ispartof><rights>Pleiades Publishing, Ltd. 2011</rights><rights>COPYRIGHT 2011 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-27d6b6d530fc767ad2879d52c0437c64b53664ca3c0184cccac85633617ef2a93</citedby><cites>FETCH-LOGICAL-c469t-27d6b6d530fc767ad2879d52c0437c64b53664ca3c0184cccac85633617ef2a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S000629791110004X$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S000629791110004X$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22098236$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yakovlev, A. G.</creatorcontrib><creatorcontrib>Vasilieva, L. G.</creatorcontrib><creatorcontrib>Shkuropatov, A. Y.</creatorcontrib><creatorcontrib>Shuvalov, V. A.</creatorcontrib><title>Coherent phenomena of charge separation in reaction centers of LL131H and LL131H/LM160H/FM197H mutants of Rhodobacter sphaeroides</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><addtitle>Biochemistry (Mosc)</addtitle><description>Primary stage of charge separation and transfer of charges was studied in reaction centers (RCs) of point mutants LL131H and LL131H/LM160H/FM197H of the purple bacterium
Rhodobacter sphaeroides
by differential absorption spectroscopy with temporal resolution of 18 fsec at 90 K. Difference absorption spectra measured at 0–4 psec delays after excitation of dimer P at 870 nm with 30 fsec step were obtained in the spectral range of 935–1060 nm. It was found that a decay of P* due to charge separation is considerably slower in the mutant RCs in comparison with native RCs of
Rba. sphaeroides
. Coherent oscillations were found in the kinetics of stimulated emission of the P* state at 940 nm. Fourier analysis of the oscillations revealed a set of characteristic bands in the frequency range of 20–500 cm
−1
. The most intense band has the frequency of −30 cm
−1
in RCs of mutant LL131H and in native RCs and the frequency of ∼100 cm
−1
in RCs of the triple mutant. It was found that an absorption band of bacteriochlorophyll anion B
A
−
which is registered in the difference absorption spectra of native RCs at 1020 nm is absent in the analogous spectra of the mutants. The results are analyzed in terms of the participation of the B
A
molecule in the primary electron transfer in the presence of a nuclear wave packet moving along the inharmonic surface of P* potential energy.</description><subject>Absorption spectroscopy</subject><subject>Bacteria</subject><subject>Bacteriochlorophylls - chemistry</subject><subject>Bacteriochlorophylls - metabolism</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Electron transfer reactions</subject><subject>Electron Transport</subject><subject>Fourier analysis</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Photosynthesis</subject><subject>Photosynthetic Reaction Center Complex Proteins - chemistry</subject><subject>Photosynthetic Reaction Center Complex Proteins - genetics</subject><subject>Photosynthetic Reaction Center Complex Proteins - metabolism</subject><subject>Phytochemistry</subject><subject>Potential energy</subject><subject>Rhodobacter sphaeroides</subject><subject>Rhodobacter sphaeroides - enzymology</subject><subject>X-Ray Absorption Spectroscopy</subject><issn>0006-2979</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk1v1DAQhi0EotvCD-CCLDj0lK6_HR-rFWWRUiHxIXGLvM5kk2pjBzs5cOSf43QXEF9CPszY87yvZqxB6BklV5RysX5PCFHMaEMpzan49ACtqCJlwYkgD9FqKRdL_Qydp3SXr4wY_hidsRxLxtUKfd2EDiL4CY8d-DCAtzi02HU27gEnGG20Ux887j2OYN197jIPMS1gVVFOt9j65pSuq9vcwnZ9c0uN3uJhnqyf7tF3XWjCLltAxGnsLMTQN5CeoEetPSR4eooX6OPNqw-bbVG9ff1mc10VTigzFUw3aqcayUnrtNK2YaU2jWSOCK6dEjvJlRLOckdoKZxz1pVSca6ohpZZwy_Q5dF3jOHzDGmqhz45OByshzCn2hBNNZf5Y_9PSqUllTqTL34j78IcfR6jLo3mTJSKZejlEdrbA9S9b8MUrVss62supDKilDJTV3-h8mlg6F3w0Pb5_RcBPQpcDClFaOsx9oONX2pK6mU96j_WI2uen_qddwM0PxTf9yED7AikXPJ7iD8H-rfrN2odv2s</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Yakovlev, A. G.</creator><creator>Vasilieva, L. G.</creator><creator>Shkuropatov, A. Y.</creator><creator>Shuvalov, V. A.</creator><general>SP MAIK Nauka/Interperiodica</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20111001</creationdate><title>Coherent phenomena of charge separation in reaction centers of LL131H and LL131H/LM160H/FM197H mutants of Rhodobacter sphaeroides</title><author>Yakovlev, A. G. ; Vasilieva, L. G. ; Shkuropatov, A. Y. ; Shuvalov, V. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-27d6b6d530fc767ad2879d52c0437c64b53664ca3c0184cccac85633617ef2a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Absorption spectroscopy</topic><topic>Bacteria</topic><topic>Bacteriochlorophylls - chemistry</topic><topic>Bacteriochlorophylls - metabolism</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Electron transfer reactions</topic><topic>Electron Transport</topic><topic>Fourier analysis</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Photosynthesis</topic><topic>Photosynthetic Reaction Center Complex Proteins - chemistry</topic><topic>Photosynthetic Reaction Center Complex Proteins - genetics</topic><topic>Photosynthetic Reaction Center Complex Proteins - metabolism</topic><topic>Phytochemistry</topic><topic>Potential energy</topic><topic>Rhodobacter sphaeroides</topic><topic>Rhodobacter sphaeroides - enzymology</topic><topic>X-Ray Absorption Spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yakovlev, A. G.</creatorcontrib><creatorcontrib>Vasilieva, L. G.</creatorcontrib><creatorcontrib>Shkuropatov, A. Y.</creatorcontrib><creatorcontrib>Shuvalov, V. A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Moscow)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yakovlev, A. G.</au><au>Vasilieva, L. G.</au><au>Shkuropatov, A. Y.</au><au>Shuvalov, V. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coherent phenomena of charge separation in reaction centers of LL131H and LL131H/LM160H/FM197H mutants of Rhodobacter sphaeroides</atitle><jtitle>Biochemistry (Moscow)</jtitle><stitle>Biochemistry Moscow</stitle><addtitle>Biochemistry (Mosc)</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>76</volume><issue>10</issue><spage>1107</spage><epage>1119</epage><pages>1107-1119</pages><issn>0006-2979</issn><eissn>1608-3040</eissn><abstract>Primary stage of charge separation and transfer of charges was studied in reaction centers (RCs) of point mutants LL131H and LL131H/LM160H/FM197H of the purple bacterium
Rhodobacter sphaeroides
by differential absorption spectroscopy with temporal resolution of 18 fsec at 90 K. Difference absorption spectra measured at 0–4 psec delays after excitation of dimer P at 870 nm with 30 fsec step were obtained in the spectral range of 935–1060 nm. It was found that a decay of P* due to charge separation is considerably slower in the mutant RCs in comparison with native RCs of
Rba. sphaeroides
. Coherent oscillations were found in the kinetics of stimulated emission of the P* state at 940 nm. Fourier analysis of the oscillations revealed a set of characteristic bands in the frequency range of 20–500 cm
−1
. The most intense band has the frequency of −30 cm
−1
in RCs of mutant LL131H and in native RCs and the frequency of ∼100 cm
−1
in RCs of the triple mutant. It was found that an absorption band of bacteriochlorophyll anion B
A
−
which is registered in the difference absorption spectra of native RCs at 1020 nm is absent in the analogous spectra of the mutants. The results are analyzed in terms of the participation of the B
A
molecule in the primary electron transfer in the presence of a nuclear wave packet moving along the inharmonic surface of P* potential energy.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><pmid>22098236</pmid><doi>10.1134/S000629791110004X</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2979 |
| ispartof | Biochemistry (Moscow), 2011-10, Vol.76 (10), p.1107-1119 |
| issn | 0006-2979 1608-3040 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_907173511 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Absorption spectroscopy Bacteria Bacteriochlorophylls - chemistry Bacteriochlorophylls - metabolism Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Electron transfer reactions Electron Transport Fourier analysis Kinetics Life Sciences Microbiology Mutants Mutation Photosynthesis Photosynthetic Reaction Center Complex Proteins - chemistry Photosynthetic Reaction Center Complex Proteins - genetics Photosynthetic Reaction Center Complex Proteins - metabolism Phytochemistry Potential energy Rhodobacter sphaeroides Rhodobacter sphaeroides - enzymology X-Ray Absorption Spectroscopy |
| title | Coherent phenomena of charge separation in reaction centers of LL131H and LL131H/LM160H/FM197H mutants of Rhodobacter sphaeroides |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T19%3A29%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Coherent%20phenomena%20of%20charge%20separation%20in%20reaction%20centers%20of%20LL131H%20and%20LL131H/LM160H/FM197H%20mutants%20of%20Rhodobacter%20sphaeroides&rft.jtitle=Biochemistry%20(Moscow)&rft.au=Yakovlev,%20A.%20G.&rft.date=2011-10-01&rft.volume=76&rft.issue=10&rft.spage=1107&rft.epage=1119&rft.pages=1107-1119&rft.issn=0006-2979&rft.eissn=1608-3040&rft_id=info:doi/10.1134/S000629791110004X&rft_dat=%3Cgale_proqu%3EA345694855%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=897324862&rft_id=info:pmid/22098236&rft_galeid=A345694855&rfr_iscdi=true |