Efficient production of antibodies against a mammalian integral membrane protein by phage display

The application of phage display technology to mammalian proteins with multiple transmembrane regions has had limited success due to the difficulty in generating these proteins in sufficient amounts and purity. We report here a method that can be easily and generally applied to sorting of phage disp...

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Veröffentlicht in:	Protein engineering, design and selection design and selection, 2011-09, Vol.24 (9), p.679-689
Hauptverfasser:	Hötzel, Isidro, Chiang, Vicki, Diao, Jingyu, Pantua, Homer, Maun, Henry R., Kapadia, Sharookh B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Baculoviridae - genetics Baculoviridae - metabolism Baculovirus Cell Line, Tumor Claudin-1 Detergents Fab Flow Cytometry HEK293 Cells Hepacivirus Hepatitis C virus Humans Immunoglobulin Fab Fragments - biosynthesis Immunoglobulin Fab Fragments - genetics Immunoglobulin G - biosynthesis Immunoglobulin G - genetics Immunoglobulins Infection Membrane proteins Membrane Proteins - biosynthesis Membrane Proteins - chemistry Membrane Proteins - immunology Mice Molecular Sequence Data Neutralization Tests Panning Peptide Library Phage display Protein Binding Protein Engineering - methods protein purification Sequence Alignment Streptavidin Virion - chemistry Virion - metabolism Virions
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