Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA
Nuclear ribosomal DNA sequence of the second internal transcribed spacer (ITS-2) has been used efficiently to identify the liver fluke species collected from different hosts and various geographic regions. ITS-2 sequences of 19 Fasciola samples collected from Korean native cattle were determined and...
Gespeichert in:
| Veröffentlicht in: | Parasitology research (1987) 2011-09, Vol.109 (3), p.833-839 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 839 |
|---|---|
| container_issue | 3 |
| container_start_page | 833 |
| container_title | Parasitology research (1987) |
| container_volume | 109 |
| creator | Choe, Se-Eun Nguyen, Thuy Thi-Dieu Kang, Tae-Gyu Kweon, Chang-Hee Kang, Seung-Won |
| description | Nuclear ribosomal DNA sequence of the second internal transcribed spacer (ITS-2) has been used efficiently to identify the liver fluke species collected from different hosts and various geographic regions. ITS-2 sequences of 19
Fasciola
samples collected from Korean native cattle were determined and compared. Sequence comparison including ITS-2 sequences of isolates from this study and reference sequences from
Fasciola hepatica
and
Fasciola gigantica
and intermediate
Fasciola
in Genbank revealed seven identical variable sites of investigated isolates. Among 19 samples, 12 individuals had ITS-2 sequences completely identical to that of pure
F. hepatica
, five possessed the sequences identical to
F. gigantica
type, whereas two shared the sequence of both
F. hepatica
and
F. gigantica
. No variations in length and nucleotide composition of ITS-2 sequence were observed within isolates that belonged to
F. hepatica
or
F. gigantica
. At the position of 218, five
Fasciola
containing a single-base substitution (C>T) formed a distinct branch inside the
F. gigantica-
type group which was similar to those of Asian-origin isolates. The phylogenetic tree of the
Fasciola
spp. based on complete ITS-2 sequences from this study and other representative isolates in different locations clearly showed that pure
F. hepatica
,
F. gigantica
type and intermediate
Fasciola
were observed. The result also provided additional genetic evidence for the existence of three forms of
Fasciola
isolated from native cattle in Korea by genetic approach using ITS-2 sequence. |
| doi_str_mv | 10.1007/s00436-011-2323-6 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_907167164</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A305996212</galeid><sourcerecordid>A305996212</sourcerecordid><originalsourceid>FETCH-LOGICAL-c510t-4b356386c14789500799ae86104857b660a73fa251c2bc3a2609cf97f9b199263</originalsourceid><addsrcrecordid>eNqFks9u1DAQxi0EosvCA3BBviDgkOL_iY-rQktFBQfK2XK8TuUqsRdPcuhb8MjMKgvcQLZsy_P7Zqz5TMhLzs45Y-17YExJ0zDOGyGFbMwjsuFKioZbrR-TDbN4xqg8I88A7hnjrVHqKTkTXFnVKb0hP69ijnMK1Gc_PkACWgZ66SGkMnqaANc5Ah1qmWjw8zxGmjL9XGr0tPcQ97RkCjGUvMfAHCumoXP1GUJNPYbh4EOs9O317bdGvEP0xxJziMcyeQlj9JUiWKBMKPzwZfecPBn8CPHFad-S75cfby8-NTdfr64vdjdN0JzNjeqlNrIzgau2sxq7Ya2PneFMdbrtjWG-lYMXmgfRB-mFYTYMth1sz60VRm7JmzXvoRZ8EsxuShDiOPocywLOspYbnOq_ZNdpYbs15_lK3vkxupSHgp0IOPZxStiiOCS830mmrTWCCxTwVRBqAahxcIeaJl8fHGfuaLFbLXZoojta7I5FXp2es_RT3P9R_PYUgdcnAG3044BmhAR_OaW6VuPX2BKxcoChfBeruy_L0T_4R_Vf3WW8kA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>885298926</pqid></control><display><type>article</type><title>Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Choe, Se-Eun ; Nguyen, Thuy Thi-Dieu ; Kang, Tae-Gyu ; Kweon, Chang-Hee ; Kang, Seung-Won</creator><creatorcontrib>Choe, Se-Eun ; Nguyen, Thuy Thi-Dieu ; Kang, Tae-Gyu ; Kweon, Chang-Hee ; Kang, Seung-Won</creatorcontrib><description>Nuclear ribosomal DNA sequence of the second internal transcribed spacer (ITS-2) has been used efficiently to identify the liver fluke species collected from different hosts and various geographic regions. ITS-2 sequences of 19
Fasciola
samples collected from Korean native cattle were determined and compared. Sequence comparison including ITS-2 sequences of isolates from this study and reference sequences from
Fasciola hepatica
and
Fasciola gigantica
and intermediate
Fasciola
in Genbank revealed seven identical variable sites of investigated isolates. Among 19 samples, 12 individuals had ITS-2 sequences completely identical to that of pure
F. hepatica
, five possessed the sequences identical to
F. gigantica
type, whereas two shared the sequence of both
F. hepatica
and
F. gigantica
. No variations in length and nucleotide composition of ITS-2 sequence were observed within isolates that belonged to
F. hepatica
or
F. gigantica
. At the position of 218, five
Fasciola
containing a single-base substitution (C>T) formed a distinct branch inside the
F. gigantica-
type group which was similar to those of Asian-origin isolates. The phylogenetic tree of the
Fasciola
spp. based on complete ITS-2 sequences from this study and other representative isolates in different locations clearly showed that pure
F. hepatica
,
F. gigantica
type and intermediate
Fasciola
were observed. The result also provided additional genetic evidence for the existence of three forms of
Fasciola
isolated from native cattle in Korea by genetic approach using ITS-2 sequence.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-011-2323-6</identifier><identifier>PMID: 21494845</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animals ; Beef cattle ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Cattle ; Cattle Diseases - parasitology ; Cluster Analysis ; DNA ; DNA, Helminth - chemistry ; DNA, Helminth - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; DNA, Ribosomal Spacer - chemistry ; DNA, Ribosomal Spacer - genetics ; Fasciola - classification ; Fasciola - genetics ; Fasciola - isolation & purification ; Fasciola gigantica ; Fasciola hepatica ; Fascioliasis - parasitology ; Fascioliasis - veterinary ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Genetic aspects ; Immunology ; Invertebrates ; Medical Microbiology ; Microbiology ; Molecular Sequence Data ; Nemathelminthia. Plathelmintha ; Original Paper ; Phylogeny ; Republic of Korea ; Sequence Analysis, DNA ; Trematoda</subject><ispartof>Parasitology research (1987), 2011-09, Vol.109 (3), p.833-839</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2011 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-4b356386c14789500799ae86104857b660a73fa251c2bc3a2609cf97f9b199263</citedby><cites>FETCH-LOGICAL-c510t-4b356386c14789500799ae86104857b660a73fa251c2bc3a2609cf97f9b199263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-011-2323-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-011-2323-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24487509$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21494845$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choe, Se-Eun</creatorcontrib><creatorcontrib>Nguyen, Thuy Thi-Dieu</creatorcontrib><creatorcontrib>Kang, Tae-Gyu</creatorcontrib><creatorcontrib>Kweon, Chang-Hee</creatorcontrib><creatorcontrib>Kang, Seung-Won</creatorcontrib><title>Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Nuclear ribosomal DNA sequence of the second internal transcribed spacer (ITS-2) has been used efficiently to identify the liver fluke species collected from different hosts and various geographic regions. ITS-2 sequences of 19
Fasciola
samples collected from Korean native cattle were determined and compared. Sequence comparison including ITS-2 sequences of isolates from this study and reference sequences from
Fasciola hepatica
and
Fasciola gigantica
and intermediate
Fasciola
in Genbank revealed seven identical variable sites of investigated isolates. Among 19 samples, 12 individuals had ITS-2 sequences completely identical to that of pure
F. hepatica
, five possessed the sequences identical to
F. gigantica
type, whereas two shared the sequence of both
F. hepatica
and
F. gigantica
. No variations in length and nucleotide composition of ITS-2 sequence were observed within isolates that belonged to
F. hepatica
or
F. gigantica
. At the position of 218, five
Fasciola
containing a single-base substitution (C>T) formed a distinct branch inside the
F. gigantica-
type group which was similar to those of Asian-origin isolates. The phylogenetic tree of the
Fasciola
spp. based on complete ITS-2 sequences from this study and other representative isolates in different locations clearly showed that pure
F. hepatica
,
F. gigantica
type and intermediate
Fasciola
were observed. The result also provided additional genetic evidence for the existence of three forms of
Fasciola
isolated from native cattle in Korea by genetic approach using ITS-2 sequence.</description><subject>Animals</subject><subject>Beef cattle</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cattle</subject><subject>Cattle Diseases - parasitology</subject><subject>Cluster Analysis</subject><subject>DNA</subject><subject>DNA, Helminth - chemistry</subject><subject>DNA, Helminth - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>DNA, Ribosomal Spacer - chemistry</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Fasciola - classification</subject><subject>Fasciola - genetics</subject><subject>Fasciola - isolation & purification</subject><subject>Fasciola gigantica</subject><subject>Fasciola hepatica</subject><subject>Fascioliasis - parasitology</subject><subject>Fascioliasis - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Genetic aspects</subject><subject>Immunology</subject><subject>Invertebrates</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>Original Paper</subject><subject>Phylogeny</subject><subject>Republic of Korea</subject><subject>Sequence Analysis, DNA</subject><subject>Trematoda</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9u1DAQxi0EosvCA3BBviDgkOL_iY-rQktFBQfK2XK8TuUqsRdPcuhb8MjMKgvcQLZsy_P7Zqz5TMhLzs45Y-17YExJ0zDOGyGFbMwjsuFKioZbrR-TDbN4xqg8I88A7hnjrVHqKTkTXFnVKb0hP69ijnMK1Gc_PkACWgZ66SGkMnqaANc5Ah1qmWjw8zxGmjL9XGr0tPcQ97RkCjGUvMfAHCumoXP1GUJNPYbh4EOs9O317bdGvEP0xxJziMcyeQlj9JUiWKBMKPzwZfecPBn8CPHFad-S75cfby8-NTdfr64vdjdN0JzNjeqlNrIzgau2sxq7Ya2PneFMdbrtjWG-lYMXmgfRB-mFYTYMth1sz60VRm7JmzXvoRZ8EsxuShDiOPocywLOspYbnOq_ZNdpYbs15_lK3vkxupSHgp0IOPZxStiiOCS830mmrTWCCxTwVRBqAahxcIeaJl8fHGfuaLFbLXZoojta7I5FXp2es_RT3P9R_PYUgdcnAG3044BmhAR_OaW6VuPX2BKxcoChfBeruy_L0T_4R_Vf3WW8kA</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Choe, Se-Eun</creator><creator>Nguyen, Thuy Thi-Dieu</creator><creator>Kang, Tae-Gyu</creator><creator>Kweon, Chang-Hee</creator><creator>Kang, Seung-Won</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20110901</creationdate><title>Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA</title><author>Choe, Se-Eun ; Nguyen, Thuy Thi-Dieu ; Kang, Tae-Gyu ; Kweon, Chang-Hee ; Kang, Seung-Won</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-4b356386c14789500799ae86104857b660a73fa251c2bc3a2609cf97f9b199263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Beef cattle</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cattle</topic><topic>Cattle Diseases - parasitology</topic><topic>Cluster Analysis</topic><topic>DNA</topic><topic>DNA, Helminth - chemistry</topic><topic>DNA, Helminth - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>DNA, Ribosomal Spacer - chemistry</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Fasciola - classification</topic><topic>Fasciola - genetics</topic><topic>Fasciola - isolation & purification</topic><topic>Fasciola gigantica</topic><topic>Fasciola hepatica</topic><topic>Fascioliasis - parasitology</topic><topic>Fascioliasis - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Genetic aspects</topic><topic>Immunology</topic><topic>Invertebrates</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>Original Paper</topic><topic>Phylogeny</topic><topic>Republic of Korea</topic><topic>Sequence Analysis, DNA</topic><topic>Trematoda</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choe, Se-Eun</creatorcontrib><creatorcontrib>Nguyen, Thuy Thi-Dieu</creatorcontrib><creatorcontrib>Kang, Tae-Gyu</creatorcontrib><creatorcontrib>Kweon, Chang-Hee</creatorcontrib><creatorcontrib>Kang, Seung-Won</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choe, Se-Eun</au><au>Nguyen, Thuy Thi-Dieu</au><au>Kang, Tae-Gyu</au><au>Kweon, Chang-Hee</au><au>Kang, Seung-Won</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>109</volume><issue>3</issue><spage>833</spage><epage>839</epage><pages>833-839</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>Nuclear ribosomal DNA sequence of the second internal transcribed spacer (ITS-2) has been used efficiently to identify the liver fluke species collected from different hosts and various geographic regions. ITS-2 sequences of 19
Fasciola
samples collected from Korean native cattle were determined and compared. Sequence comparison including ITS-2 sequences of isolates from this study and reference sequences from
Fasciola hepatica
and
Fasciola gigantica
and intermediate
Fasciola
in Genbank revealed seven identical variable sites of investigated isolates. Among 19 samples, 12 individuals had ITS-2 sequences completely identical to that of pure
F. hepatica
, five possessed the sequences identical to
F. gigantica
type, whereas two shared the sequence of both
F. hepatica
and
F. gigantica
. No variations in length and nucleotide composition of ITS-2 sequence were observed within isolates that belonged to
F. hepatica
or
F. gigantica
. At the position of 218, five
Fasciola
containing a single-base substitution (C>T) formed a distinct branch inside the
F. gigantica-
type group which was similar to those of Asian-origin isolates. The phylogenetic tree of the
Fasciola
spp. based on complete ITS-2 sequences from this study and other representative isolates in different locations clearly showed that pure
F. hepatica
,
F. gigantica
type and intermediate
Fasciola
were observed. The result also provided additional genetic evidence for the existence of three forms of
Fasciola
isolated from native cattle in Korea by genetic approach using ITS-2 sequence.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21494845</pmid><doi>10.1007/s00436-011-2323-6</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2011-09, Vol.109 (3), p.833-839 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_907167164 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Beef cattle Biological and medical sciences Biomedical and Life Sciences Biomedicine Cattle Cattle Diseases - parasitology Cluster Analysis DNA DNA, Helminth - chemistry DNA, Helminth - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics DNA, Ribosomal Spacer - chemistry DNA, Ribosomal Spacer - genetics Fasciola - classification Fasciola - genetics Fasciola - isolation & purification Fasciola gigantica Fasciola hepatica Fascioliasis - parasitology Fascioliasis - veterinary Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Genetic aspects Immunology Invertebrates Medical Microbiology Microbiology Molecular Sequence Data Nemathelminthia. Plathelmintha Original Paper Phylogeny Republic of Korea Sequence Analysis, DNA Trematoda |
| title | Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T08%3A55%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20analysis%20of%20Fasciola%20isolates%20from%20cattle%20in%20Korea%20based%20on%20second%20internal%20transcribed%20spacer%20(ITS-2)%20sequence%20of%20nuclear%20ribosomal%20DNA&rft.jtitle=Parasitology%20research%20(1987)&rft.au=Choe,%20Se-Eun&rft.date=2011-09-01&rft.volume=109&rft.issue=3&rft.spage=833&rft.epage=839&rft.pages=833-839&rft.issn=0932-0113&rft.eissn=1432-1955&rft.coden=PARREZ&rft_id=info:doi/10.1007/s00436-011-2323-6&rft_dat=%3Cgale_proqu%3EA305996212%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=885298926&rft_id=info:pmid/21494845&rft_galeid=A305996212&rfr_iscdi=true |