Dasytricha Dominance in Surti Buffalo Rumen Revealed by 18S rRNA Sequences and Real-Time PCR Assay

The genetic diversity of protozoa in Surti buffalo rumen was studied by amplified ribosomal DNA restriction analysis, 18S rDNA sequence homology and phylogenetic and Real-time PCR analysis methods. Three animals were fed diet comprised green fodder Napier bajra 21 (Pennisetum purpureum), mature past...

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Veröffentlicht in:	Current microbiology 2011-09, Vol.63 (3), p.281-288
Hauptverfasser:	Singh, K. M, Tripathi, A. K, Pandya, P. R, Rank, D. N, Kothari, R. K, Joshi, C. G
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biodiversity Biomedical and Life Sciences Biotechnology Buffalo Buffaloes Ciliophora - classification Ciliophora - genetics Ciliophora - growth & development clones Cluster Analysis crude protein Dasytricha Dasytricha ruminantium Diet Digestive system DNA, Protozoan - chemistry DNA, Protozoan - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Entodinium Fodder forage genes Genes, rRNA Genetic diversity genetic variation grasses Isotricha Life Sciences Metagenome Microbiology Molecular Sequence Data nucleotide sequences nutrients Pasture pastures Pennisetum purpureum Phylogenetics Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Polyplastron Protozoa quantitative polymerase chain reaction ribosomal DNA ribosomal RNA RNA, Protozoan - genetics RNA, Ribosomal, 18S - genetics rumen Rumen - parasitology Sequence Analysis, DNA sequence homology
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