Choline catabolism, σ 54 factor and NtrC are required for the full expression of the Pseudomonas aeruginosa phosphorylcholine phosphatase gene
Choline favors the pathogenesis of Pseudomonas aeruginosa because hemolytic phospholipase C and phosphorylcholine phosphatase (PchP) are synthesized as a consequence of its catabolism. The experiments performed here resulted in the identification of the factors that regulate both the catabolism of c...
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| Veröffentlicht in: | Microbiological research 2011-07, Vol.166 (5), p.380-390 |
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| container_title | Microbiological research |
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| creator | Massimelli, Maria J. Sánchez, Diego G. Buchieri, Maria V. Olvera, Leticia Beassoni, Paola R. Schweizer, Herbert P. Morett, Enrique Lisa, Angela T. |
| description | Choline favors the pathogenesis of
Pseudomonas aeruginosa because hemolytic phospholipase C and phosphorylcholine phosphatase (PchP) are synthesized as a consequence of its catabolism. The experiments performed here resulted in the identification of the factors that regulate both the catabolism of choline and the gene coding for PchP. We have also identified and characterized the promoter of the
pchP gene, its transcriptional organization and the factors that affect its expression. Deletion analyses reveal that the region between −188 and −68 contains all controlling elements necessary for
pchP expression: a hypothetical −12/−24 promoter element, a consensus sequence for the integration host factor (−141/−133), and a palindromic sequence resembling a binding site for a potential enhancer binding protein (−190/−174). Our data also demonstrate that choline catabolism and NtrC (nitrogen regulatory protein) are necessary for the full expression of
pchP and is partially dependent on σ
54 factor. |
| doi_str_mv | 10.1016/j.micres.2010.07.004 |
| format | Article |
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Pseudomonas aeruginosa because hemolytic phospholipase C and phosphorylcholine phosphatase (PchP) are synthesized as a consequence of its catabolism. The experiments performed here resulted in the identification of the factors that regulate both the catabolism of choline and the gene coding for PchP. We have also identified and characterized the promoter of the
pchP gene, its transcriptional organization and the factors that affect its expression. Deletion analyses reveal that the region between −188 and −68 contains all controlling elements necessary for
pchP expression: a hypothetical −12/−24 promoter element, a consensus sequence for the integration host factor (−141/−133), and a palindromic sequence resembling a binding site for a potential enhancer binding protein (−190/−174). Our data also demonstrate that choline catabolism and NtrC (nitrogen regulatory protein) are necessary for the full expression of
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Pseudomonas aeruginosa because hemolytic phospholipase C and phosphorylcholine phosphatase (PchP) are synthesized as a consequence of its catabolism. The experiments performed here resulted in the identification of the factors that regulate both the catabolism of choline and the gene coding for PchP. We have also identified and characterized the promoter of the
pchP gene, its transcriptional organization and the factors that affect its expression. Deletion analyses reveal that the region between −188 and −68 contains all controlling elements necessary for
pchP expression: a hypothetical −12/−24 promoter element, a consensus sequence for the integration host factor (−141/−133), and a palindromic sequence resembling a binding site for a potential enhancer binding protein (−190/−174). Our data also demonstrate that choline catabolism and NtrC (nitrogen regulatory protein) are necessary for the full expression of
pchP and is partially dependent on σ
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Pseudomonas aeruginosa because hemolytic phospholipase C and phosphorylcholine phosphatase (PchP) are synthesized as a consequence of its catabolism. The experiments performed here resulted in the identification of the factors that regulate both the catabolism of choline and the gene coding for PchP. We have also identified and characterized the promoter of the
pchP gene, its transcriptional organization and the factors that affect its expression. Deletion analyses reveal that the region between −188 and −68 contains all controlling elements necessary for
pchP expression: a hypothetical −12/−24 promoter element, a consensus sequence for the integration host factor (−141/−133), and a palindromic sequence resembling a binding site for a potential enhancer binding protein (−190/−174). Our data also demonstrate that choline catabolism and NtrC (nitrogen regulatory protein) are necessary for the full expression of
pchP and is partially dependent on σ
54 factor.</abstract><pub>Elsevier GmbH</pub><doi>10.1016/j.micres.2010.07.004</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Choline Phosphorylcholine phosphatase Pseudomonas aeruginosa Regulation |
| title | Choline catabolism, σ 54 factor and NtrC are required for the full expression of the Pseudomonas aeruginosa phosphorylcholine phosphatase gene |
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