Galleria mellonella as an infection model for Campylobacter jejuni virulence
Larvae of Galleria mellonella (Greater Wax Moth) have been shown to be susceptible to Campylobacter jejuni infection and our study characterizes this infection model. Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was...
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| Veröffentlicht in: | Journal of medical microbiology 2011-05, Vol.60 (5), p.661-669 |
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| container_title | Journal of medical microbiology |
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| creator | SENIOR, Nicola J BAGNALL, Mary C CHAMPION, Olivia L REYNOLDS, Stuart E LA RAGIONE, Roberto M WOODWARD, Martin J SALGUERO, Francisco J TITBALL, Richard W |
| description | Larvae of Galleria mellonella (Greater Wax Moth) have been shown to be susceptible to Campylobacter jejuni infection and our study characterizes this infection model. Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was extensive damage to the gut. Bacteria were found in the extracellular and cell-associated fraction in the haemocoel, and it was shown that C. jejuni can survive in insect cells. Finally, we have used the model to screen a further 67 C. jejuni isolates belonging to different MLST types. Isolates belonging to ST257 were the most virulent in the Galleria model, whereas those belonging to ST21 were the least virulent. |
| doi_str_mv | 10.1099/jmm.0.026658-0 |
| format | Article |
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Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was extensive damage to the gut. Bacteria were found in the extracellular and cell-associated fraction in the haemocoel, and it was shown that C. jejuni can survive in insect cells. Finally, we have used the model to screen a further 67 C. jejuni isolates belonging to different MLST types. Isolates belonging to ST257 were the most virulent in the Galleria model, whereas those belonging to ST21 were the least virulent.</description><identifier>ISSN: 0022-2615</identifier><identifier>EISSN: 1473-5644</identifier><identifier>DOI: 10.1099/jmm.0.026658-0</identifier><identifier>PMID: 21233296</identifier><identifier>CODEN: JMMIAV</identifier><language>eng</language><publisher>Reading: Society for General Microbiology</publisher><subject>Animals ; Bacterial Typing Techniques ; Biological and medical sciences ; Campylobacter Infections - etiology ; Campylobacter jejuni ; Campylobacter jejuni - classification ; Campylobacter jejuni - genetics ; Campylobacter jejuni - isolation & purification ; Campylobacter jejuni - pathogenicity ; Cell Line ; Digestive tract ; Disease Models, Animal ; Fundamental and applied biological sciences. Psychology ; Galleria ; Galleria mellonella ; Hemocytes - microbiology ; Humans ; Infection ; Infectious diseases ; Insect cells ; Larva - microbiology ; Macrophages - microbiology ; Medical sciences ; Mice ; Microbiology ; Moths - microbiology ; Multilocus Sequence Typing ; Spodoptera - microbiology ; Virulence ; Virulence - genetics</subject><ispartof>Journal of medical microbiology, 2011-05, Vol.60 (5), p.661-669</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-57dcd233bc8a9ce56aae284a84e466d41d7ced745797edf921b3a79480ec3e393</citedby><cites>FETCH-LOGICAL-c396t-57dcd233bc8a9ce56aae284a84e466d41d7ced745797edf921b3a79480ec3e393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24148110$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21233296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SENIOR, Nicola J</creatorcontrib><creatorcontrib>BAGNALL, Mary C</creatorcontrib><creatorcontrib>CHAMPION, Olivia L</creatorcontrib><creatorcontrib>REYNOLDS, Stuart E</creatorcontrib><creatorcontrib>LA RAGIONE, Roberto M</creatorcontrib><creatorcontrib>WOODWARD, Martin J</creatorcontrib><creatorcontrib>SALGUERO, Francisco J</creatorcontrib><creatorcontrib>TITBALL, Richard W</creatorcontrib><title>Galleria mellonella as an infection model for Campylobacter jejuni virulence</title><title>Journal of medical microbiology</title><addtitle>J Med Microbiol</addtitle><description>Larvae of Galleria mellonella (Greater Wax Moth) have been shown to be susceptible to Campylobacter jejuni infection and our study characterizes this infection model. Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was extensive damage to the gut. Bacteria were found in the extracellular and cell-associated fraction in the haemocoel, and it was shown that C. jejuni can survive in insect cells. Finally, we have used the model to screen a further 67 C. jejuni isolates belonging to different MLST types. Isolates belonging to ST257 were the most virulent in the Galleria model, whereas those belonging to ST21 were the least virulent.</description><subject>Animals</subject><subject>Bacterial Typing Techniques</subject><subject>Biological and medical sciences</subject><subject>Campylobacter Infections - etiology</subject><subject>Campylobacter jejuni</subject><subject>Campylobacter jejuni - classification</subject><subject>Campylobacter jejuni - genetics</subject><subject>Campylobacter jejuni - isolation & purification</subject><subject>Campylobacter jejuni - pathogenicity</subject><subject>Cell Line</subject><subject>Digestive tract</subject><subject>Disease Models, Animal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galleria</subject><subject>Galleria mellonella</subject><subject>Hemocytes - microbiology</subject><subject>Humans</subject><subject>Infection</subject><subject>Infectious diseases</subject><subject>Insect cells</subject><subject>Larva - microbiology</subject><subject>Macrophages - microbiology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Moths - microbiology</subject><subject>Multilocus Sequence Typing</subject><subject>Spodoptera - microbiology</subject><subject>Virulence</subject><subject>Virulence - genetics</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAUhS0EgvJYGZEXxJRy_Ygdj6jiJVVigdm6dW6kVE5S7AaJf09QC4ws9y7fOTr6GLsUMBfg3O266-YwB2lMWRVwwGZCW1WURutDNgOQspBGlCfsNOc1gLBKuWN2IoVUSjozY8tHjJFSi7yjGId-Osgxc-x52zcUtu3Q826oKfJmSHyB3eYzDisMW0p8Teuxb_lHm8ZIfaBzdtRgzHSx_2fs7eH-dfFULF8enxd3yyIoZ7ZFaetQTwNWoUIXqDSIJCuNlSZtTK1FbQPVVpfWWaobJ8VKoXW6AgqKlFNn7GbXu0nD-0h567s2h-_pPQ1j9g6sMAaE-pesjNJSW7ATOd-RIQ05J2r8JrUdpk8vwH-r9pNqD36n2sMUuNpXj6uO6l_8x-0EXO8BzAFjk7APbf7jtNCVEKC-ADnDhwc</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>SENIOR, Nicola J</creator><creator>BAGNALL, Mary C</creator><creator>CHAMPION, Olivia L</creator><creator>REYNOLDS, Stuart E</creator><creator>LA RAGIONE, Roberto M</creator><creator>WOODWARD, Martin J</creator><creator>SALGUERO, Francisco J</creator><creator>TITBALL, Richard W</creator><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20110501</creationdate><title>Galleria mellonella as an infection model for Campylobacter jejuni virulence</title><author>SENIOR, Nicola J ; BAGNALL, Mary C ; CHAMPION, Olivia L ; REYNOLDS, Stuart E ; LA RAGIONE, Roberto M ; WOODWARD, Martin J ; SALGUERO, Francisco J ; TITBALL, Richard W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-57dcd233bc8a9ce56aae284a84e466d41d7ced745797edf921b3a79480ec3e393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Bacterial Typing Techniques</topic><topic>Biological and medical sciences</topic><topic>Campylobacter Infections - etiology</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - classification</topic><topic>Campylobacter jejuni - genetics</topic><topic>Campylobacter jejuni - isolation & purification</topic><topic>Campylobacter jejuni - pathogenicity</topic><topic>Cell Line</topic><topic>Digestive tract</topic><topic>Disease Models, Animal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galleria</topic><topic>Galleria mellonella</topic><topic>Hemocytes - microbiology</topic><topic>Humans</topic><topic>Infection</topic><topic>Infectious diseases</topic><topic>Insect cells</topic><topic>Larva - microbiology</topic><topic>Macrophages - microbiology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Moths - microbiology</topic><topic>Multilocus Sequence Typing</topic><topic>Spodoptera - microbiology</topic><topic>Virulence</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SENIOR, Nicola J</creatorcontrib><creatorcontrib>BAGNALL, Mary C</creatorcontrib><creatorcontrib>CHAMPION, Olivia L</creatorcontrib><creatorcontrib>REYNOLDS, Stuart E</creatorcontrib><creatorcontrib>LA RAGIONE, Roberto M</creatorcontrib><creatorcontrib>WOODWARD, Martin J</creatorcontrib><creatorcontrib>SALGUERO, Francisco J</creatorcontrib><creatorcontrib>TITBALL, Richard W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SENIOR, Nicola J</au><au>BAGNALL, Mary C</au><au>CHAMPION, Olivia L</au><au>REYNOLDS, Stuart E</au><au>LA RAGIONE, Roberto M</au><au>WOODWARD, Martin J</au><au>SALGUERO, Francisco J</au><au>TITBALL, Richard W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Galleria mellonella as an infection model for Campylobacter jejuni virulence</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>60</volume><issue>5</issue><spage>661</spage><epage>669</epage><pages>661-669</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><coden>JMMIAV</coden><abstract>Larvae of Galleria mellonella (Greater Wax Moth) have been shown to be susceptible to Campylobacter jejuni infection and our study characterizes this infection model. Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was extensive damage to the gut. Bacteria were found in the extracellular and cell-associated fraction in the haemocoel, and it was shown that C. jejuni can survive in insect cells. Finally, we have used the model to screen a further 67 C. jejuni isolates belonging to different MLST types. Isolates belonging to ST257 were the most virulent in the Galleria model, whereas those belonging to ST21 were the least virulent.</abstract><cop>Reading</cop><pub>Society for General Microbiology</pub><pmid>21233296</pmid><doi>10.1099/jmm.0.026658-0</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Bacterial Typing Techniques Biological and medical sciences Campylobacter Infections - etiology Campylobacter jejuni Campylobacter jejuni - classification Campylobacter jejuni - genetics Campylobacter jejuni - isolation & purification Campylobacter jejuni - pathogenicity Cell Line Digestive tract Disease Models, Animal Fundamental and applied biological sciences. Psychology Galleria Galleria mellonella Hemocytes - microbiology Humans Infection Infectious diseases Insect cells Larva - microbiology Macrophages - microbiology Medical sciences Mice Microbiology Moths - microbiology Multilocus Sequence Typing Spodoptera - microbiology Virulence Virulence - genetics |
| title | Galleria mellonella as an infection model for Campylobacter jejuni virulence |
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