Fermentation of mucin by bifidobacteria from rectal samples of humans and rectal and intestinal samples of animals
Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 b...
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| Veröffentlicht in: | Folia microbiologica 2011-03, Vol.56 (2), p.85-89 |
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| description | Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial strains of animal origin. The difference between human and animal isolates in ability to grow on mucin was significant at
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| doi_str_mv | 10.1007/s12223-011-0022-4 |
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p
< 0.001. Nine human strains with the best growth on mucin were identified as
Bifidobacterium bifidum
. These strains produced extracellular, membrane-bound, and intracellular mucinases with activities of 0.11, 0.53, and 0.09 μmol/min of reducing sugars per milligram of protein, respectively. Membrane-bound mucinases were active between pH 5 and 10. The optimum pH of extracellular mucinases was 6–7. Fermentation patterns in cultures grown on mucin and glucose differed. On mucin, the acetate-to-lactate ratio was higher than in cultures grown on glucose (
p =
0.012). We showed that the bifidobacteria belong to the mucin-fermenting bacteria in humans, but their significance in mucin degradation in animals seems to be limited.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/s12223-011-0022-4</identifier><identifier>PMID: 21468760</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adult ; Animals ; Applied Microbiology ; Bacteria ; Bifidobacterium - enzymology ; Bifidobacterium - genetics ; Bifidobacterium - isolation & purification ; Bifidobacterium - metabolism ; Bifidobacterium bifidum ; Biomedical and Life Sciences ; Cattle ; Environmental Engineering/Biotechnology ; Enzyme Stability ; Fermentation ; Glucose - metabolism ; Goats ; Humans ; Hydrogen-Ion Concentration ; Immunology ; Infant ; Infants ; Intestine, Large - microbiology ; Life Sciences ; Microbiology ; Mucins - metabolism ; Mucous membrane ; Polysaccharide-Lyases - chemistry ; Polysaccharide-Lyases - metabolism ; Temperature</subject><ispartof>Folia microbiologica, 2011-03, Vol.56 (2), p.85-89</ispartof><rights>Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2011</rights><rights>Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-a89b2a31a1b3ca69a3fcdfb421dd378e681d7cae861ffff1bd8ee63c54ee27a93</citedby><cites>FETCH-LOGICAL-c402t-a89b2a31a1b3ca69a3fcdfb421dd378e681d7cae861ffff1bd8ee63c54ee27a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12223-011-0022-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12223-011-0022-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21468760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Killer, J.</creatorcontrib><creatorcontrib>Marounek, M.</creatorcontrib><title>Fermentation of mucin by bifidobacteria from rectal samples of humans and rectal and intestinal samples of animals</title><title>Folia microbiologica</title><addtitle>Folia Microbiol</addtitle><addtitle>Folia Microbiol (Praha)</addtitle><description>Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial strains of animal origin. The difference between human and animal isolates in ability to grow on mucin was significant at
p
< 0.001. Nine human strains with the best growth on mucin were identified as
Bifidobacterium bifidum
. These strains produced extracellular, membrane-bound, and intracellular mucinases with activities of 0.11, 0.53, and 0.09 μmol/min of reducing sugars per milligram of protein, respectively. Membrane-bound mucinases were active between pH 5 and 10. The optimum pH of extracellular mucinases was 6–7. Fermentation patterns in cultures grown on mucin and glucose differed. On mucin, the acetate-to-lactate ratio was higher than in cultures grown on glucose (
p =
0.012). We showed that the bifidobacteria belong to the mucin-fermenting bacteria in humans, but their significance in mucin degradation in animals seems to be limited.</description><subject>Adult</subject><subject>Animals</subject><subject>Applied Microbiology</subject><subject>Bacteria</subject><subject>Bifidobacterium - enzymology</subject><subject>Bifidobacterium - genetics</subject><subject>Bifidobacterium - isolation & purification</subject><subject>Bifidobacterium - metabolism</subject><subject>Bifidobacterium bifidum</subject><subject>Biomedical and Life Sciences</subject><subject>Cattle</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Enzyme Stability</subject><subject>Fermentation</subject><subject>Glucose - metabolism</subject><subject>Goats</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunology</subject><subject>Infant</subject><subject>Infants</subject><subject>Intestine, Large - microbiology</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Mucins - metabolism</subject><subject>Mucous membrane</subject><subject>Polysaccharide-Lyases - chemistry</subject><subject>Polysaccharide-Lyases - metabolism</subject><subject>Temperature</subject><issn>0015-5632</issn><issn>1874-9356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkcFO3DAQhq2qqCzQB-CCol56SvHYjp0cKwQtEhIXOFsTZ9IaJc5iJwfeHke7W9RKqL6Mpfn82-OPsXPg34Bzc5lACCFLDlByLkSpPrAN1EaVjaz0R7bhHKqy0lIcs5OUnjjXXEnxiR0LULo2mm9YvKE4Uphx9lMopr4YF-dD0b4Ure99N7XoZooeiz5OYxHJzTgUCcftQGnFfy8jhlRg6A7NdevDTGn24W8Wgx9xSGfsqM-FPu_rKXu8uX64-lne3f-4vfp-VzrFxVxi3bQCJSC00qFuUPau61sloOukqUnX0BmHVGvo84K2q4m0dJUiEgYbecq-7nK3cXpe8nvs6JOjYcBA05Jsww1opTX8l6xN_m5RSZXJL_-QT9MS85wrxJUSVWMyBDvIxSmlSL3dxjx5fLHA7SrO7sTZLM6u4uwafLEPXtqRuj8nDqYyIHZAyq3wi-Lbze-nvgJYZ6S-</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Killer, J.</creator><creator>Marounek, M.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20110301</creationdate><title>Fermentation of mucin by bifidobacteria from rectal samples of humans and rectal and intestinal samples of animals</title><author>Killer, J. ; Marounek, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-a89b2a31a1b3ca69a3fcdfb421dd378e681d7cae861ffff1bd8ee63c54ee27a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Applied Microbiology</topic><topic>Bacteria</topic><topic>Bifidobacterium - enzymology</topic><topic>Bifidobacterium - genetics</topic><topic>Bifidobacterium - isolation & purification</topic><topic>Bifidobacterium - metabolism</topic><topic>Bifidobacterium bifidum</topic><topic>Biomedical and Life Sciences</topic><topic>Cattle</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Enzyme Stability</topic><topic>Fermentation</topic><topic>Glucose - metabolism</topic><topic>Goats</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunology</topic><topic>Infant</topic><topic>Infants</topic><topic>Intestine, Large - microbiology</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Mucins - metabolism</topic><topic>Mucous membrane</topic><topic>Polysaccharide-Lyases - chemistry</topic><topic>Polysaccharide-Lyases - metabolism</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Killer, J.</creatorcontrib><creatorcontrib>Marounek, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Killer, J.</au><au>Marounek, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fermentation of mucin by bifidobacteria from rectal samples of humans and rectal and intestinal samples of animals</atitle><jtitle>Folia microbiologica</jtitle><stitle>Folia Microbiol</stitle><addtitle>Folia Microbiol (Praha)</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>56</volume><issue>2</issue><spage>85</spage><epage>89</epage><pages>85-89</pages><issn>0015-5632</issn><eissn>1874-9356</eissn><abstract>Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial strains of animal origin. The difference between human and animal isolates in ability to grow on mucin was significant at
p
< 0.001. Nine human strains with the best growth on mucin were identified as
Bifidobacterium bifidum
. These strains produced extracellular, membrane-bound, and intracellular mucinases with activities of 0.11, 0.53, and 0.09 μmol/min of reducing sugars per milligram of protein, respectively. Membrane-bound mucinases were active between pH 5 and 10. The optimum pH of extracellular mucinases was 6–7. Fermentation patterns in cultures grown on mucin and glucose differed. On mucin, the acetate-to-lactate ratio was higher than in cultures grown on glucose (
p =
0.012). We showed that the bifidobacteria belong to the mucin-fermenting bacteria in humans, but their significance in mucin degradation in animals seems to be limited.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21468760</pmid><doi>10.1007/s12223-011-0022-4</doi><tpages>5</tpages></addata></record> |
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| ispartof | Folia microbiologica, 2011-03, Vol.56 (2), p.85-89 |
| issn | 0015-5632 1874-9356 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult Animals Applied Microbiology Bacteria Bifidobacterium - enzymology Bifidobacterium - genetics Bifidobacterium - isolation & purification Bifidobacterium - metabolism Bifidobacterium bifidum Biomedical and Life Sciences Cattle Environmental Engineering/Biotechnology Enzyme Stability Fermentation Glucose - metabolism Goats Humans Hydrogen-Ion Concentration Immunology Infant Infants Intestine, Large - microbiology Life Sciences Microbiology Mucins - metabolism Mucous membrane Polysaccharide-Lyases - chemistry Polysaccharide-Lyases - metabolism Temperature |
| title | Fermentation of mucin by bifidobacteria from rectal samples of humans and rectal and intestinal samples of animals |
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