Identification and characterization of myeloma-associated antigens in Trichinella spiralis

[Display omitted] ► Trichinella spiralis has myeloma-associated antigens. ► The myeloma-associated antigen tropomyosin (TM) in T. spiralis was identified. ► TM has the anti-tumor effect in mice. To investigate the presence of myeloma-associated antigens in Trichinella spiralis and their anti-tumor effect, cross-immune responses between antigens of the myeloma cell SP2/0 versus positive sera to T. spiralis, and antigens of T. spiralis versus positive sera to myeloma cell SP2/0 were determined using T. spiralis and myeloma specific enzyme-linked immunosorbent assays (ELISA). The myeloma-associated antigens in T. spiralis were separated by ultrafiltration and 2-D electrophoresis, and the amino acid sequences and molecular weights were determined by spectrometry. An obvious reaction was found between a 33kDa antigen and positive sera, and the major component of the antigen was tropomyosin (TM), which is an surface acidic protein with 284 amino acids. Mice were immunized with TM to determine the anti-tumor effect in vivo. The results showed that CD4+, CD8+ T lymphocyte, and CD19+ B lymphocyte were significantly increased (P