Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia
Molecular typing and resistotyping coupled with gyrA single nucleotide polymorphism (SNP) of 60 Salmonella Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primer...
Gespeichert in:
| Veröffentlicht in: | Folia microbiologica 2011, Vol.56 (1), p.66-71 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 71 |
|---|---|
| container_issue | 1 |
| container_start_page | 66 |
| container_title | Folia microbiologica |
| container_volume | 56 |
| creator | Kozoderović, G. Velhner, M. Jelesić, Z. Stojanov, I. Petrović, T. Stojanović, D. Golić, N. |
| description | Molecular typing and resistotyping coupled with
gyrA
single nucleotide polymorphism (SNP) of 60
Salmonella
Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (
D
) was 0.688. In combination with resistotyping and
gyrA
SNP,
D
increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp
87
→ Asn in six strains, Asp
87
→ Gly in one strain, and Ser
83
→ Phe in one strain. One NAL-resistant strain had no mutations in QRDR, suggesting another mechanism of resistance. |
| doi_str_mv | 10.1007/s12223-011-0003-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_907161286</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2377071461</sourcerecordid><originalsourceid>FETCH-LOGICAL-c402t-816d090cdc495bb4737c4b2cee4153ed6db87802cc2211a0280bb92f890afc983</originalsourceid><addsrcrecordid>eNp1kU2PFCEQhonRuOPqD_BiiBcv21oF3Q19NJv1I1njYfVMaKBXNjSMQB_m3y_jrJqYeCB14KmnKvUS8hLhLQKIdwUZY7wDxA4AeCcekR1K0XcTH8bHZAeAQzeMnJ2RZ6XcAYzQc_aUnDHkU9-LYUcOX1JwZgs603rY-3hLdbTtVb96k9PsdaDZFV-qjsbRtNAbHdYUXQiaXsXqsq_e-kJ9SUFXZ-mS00r3aQs1Hy7okpK9-OX8sa06Ni7SG5eb9zl5suhQ3IuHek6-f7j6dvmpu_768fPl--vO9MBqJ3G0MIGxpp-Gee4FF6afmXGux4E7O9pZCgnMGMYQNTAJ8zyxRU6gFzNJfk7enLz7nH5urlS1-mKO-0eXtqImEDgik2MjX_9D3qUtx7ackoKhYBKxQXiC2nVKyW5R--xXnQ8KQR1TUadUVEtFHVNRovW8ehBv8-rsn47fMTSAnYDSvuKty38n_996D1E9mF4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>872172811</pqid></control><display><type>article</type><title>Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia</title><source>MEDLINE</source><source>Springer Online Journals</source><creator>Kozoderović, G. ; Velhner, M. ; Jelesić, Z. ; Stojanov, I. ; Petrović, T. ; Stojanović, D. ; Golić, N.</creator><creatorcontrib>Kozoderović, G. ; Velhner, M. ; Jelesić, Z. ; Stojanov, I. ; Petrović, T. ; Stojanović, D. ; Golić, N.</creatorcontrib><description>Molecular typing and resistotyping coupled with
gyrA
single nucleotide polymorphism (SNP) of 60
Salmonella
Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (
D
) was 0.688. In combination with resistotyping and
gyrA
SNP,
D
increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp
87
→ Asn in six strains, Asp
87
→ Gly in one strain, and Ser
83
→ Phe in one strain. One NAL-resistant strain had no mutations in QRDR, suggesting another mechanism of resistance.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/s12223-011-0003-7</identifier><identifier>PMID: 21394475</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Applied Microbiology ; Bacterial Proteins - genetics ; Biomedical and Life Sciences ; Chickens ; DNA Gyrase - genetics ; Drug resistance ; Drug Resistance, Bacterial ; Environmental Engineering/Biotechnology ; Fast food ; Food ; Food Microbiology ; Humans ; Immunology ; Life Sciences ; Microbial Sensitivity Tests ; Microbiology ; Molecular biology ; Molecular Typing ; Mutation ; Polymorphism ; Polymorphism, Single Nucleotide ; Poultry ; Poultry Diseases - microbiology ; Salmonella ; Salmonella enteritidis ; Salmonella enteritidis - classification ; Salmonella enteritidis - drug effects ; Salmonella enteritidis - genetics ; Salmonella enteritidis - isolation & purification ; Salmonella Infections - microbiology ; Salmonella Infections, Animal - microbiology ; Serbia</subject><ispartof>Folia microbiologica, 2011, Vol.56 (1), p.66-71</ispartof><rights>Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2011</rights><rights>Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-816d090cdc495bb4737c4b2cee4153ed6db87802cc2211a0280bb92f890afc983</citedby><cites>FETCH-LOGICAL-c402t-816d090cdc495bb4737c4b2cee4153ed6db87802cc2211a0280bb92f890afc983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12223-011-0003-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12223-011-0003-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21394475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kozoderović, G.</creatorcontrib><creatorcontrib>Velhner, M.</creatorcontrib><creatorcontrib>Jelesić, Z.</creatorcontrib><creatorcontrib>Stojanov, I.</creatorcontrib><creatorcontrib>Petrović, T.</creatorcontrib><creatorcontrib>Stojanović, D.</creatorcontrib><creatorcontrib>Golić, N.</creatorcontrib><title>Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia</title><title>Folia microbiologica</title><addtitle>Folia Microbiol</addtitle><addtitle>Folia Microbiol (Praha)</addtitle><description>Molecular typing and resistotyping coupled with
gyrA
single nucleotide polymorphism (SNP) of 60
Salmonella
Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (
D
) was 0.688. In combination with resistotyping and
gyrA
SNP,
D
increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp
87
→ Asn in six strains, Asp
87
→ Gly in one strain, and Ser
83
→ Phe in one strain. One NAL-resistant strain had no mutations in QRDR, suggesting another mechanism of resistance.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Applied Microbiology</subject><subject>Bacterial Proteins - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Chickens</subject><subject>DNA Gyrase - genetics</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Fast food</subject><subject>Food</subject><subject>Food Microbiology</subject><subject>Humans</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Molecular biology</subject><subject>Molecular Typing</subject><subject>Mutation</subject><subject>Polymorphism</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Poultry</subject><subject>Poultry Diseases - microbiology</subject><subject>Salmonella</subject><subject>Salmonella enteritidis</subject><subject>Salmonella enteritidis - classification</subject><subject>Salmonella enteritidis - drug effects</subject><subject>Salmonella enteritidis - genetics</subject><subject>Salmonella enteritidis - isolation & purification</subject><subject>Salmonella Infections - microbiology</subject><subject>Salmonella Infections, Animal - microbiology</subject><subject>Serbia</subject><issn>0015-5632</issn><issn>1874-9356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kU2PFCEQhonRuOPqD_BiiBcv21oF3Q19NJv1I1njYfVMaKBXNjSMQB_m3y_jrJqYeCB14KmnKvUS8hLhLQKIdwUZY7wDxA4AeCcekR1K0XcTH8bHZAeAQzeMnJ2RZ6XcAYzQc_aUnDHkU9-LYUcOX1JwZgs603rY-3hLdbTtVb96k9PsdaDZFV-qjsbRtNAbHdYUXQiaXsXqsq_e-kJ9SUFXZ-mS00r3aQs1Hy7okpK9-OX8sa06Ni7SG5eb9zl5suhQ3IuHek6-f7j6dvmpu_768fPl--vO9MBqJ3G0MIGxpp-Gee4FF6afmXGux4E7O9pZCgnMGMYQNTAJ8zyxRU6gFzNJfk7enLz7nH5urlS1-mKO-0eXtqImEDgik2MjX_9D3qUtx7ackoKhYBKxQXiC2nVKyW5R--xXnQ8KQR1TUadUVEtFHVNRovW8ehBv8-rsn47fMTSAnYDSvuKty38n_996D1E9mF4</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Kozoderović, G.</creator><creator>Velhner, M.</creator><creator>Jelesić, Z.</creator><creator>Stojanov, I.</creator><creator>Petrović, T.</creator><creator>Stojanović, D.</creator><creator>Golić, N.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>2011</creationdate><title>Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia</title><author>Kozoderović, G. ; Velhner, M. ; Jelesić, Z. ; Stojanov, I. ; Petrović, T. ; Stojanović, D. ; Golić, N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-816d090cdc495bb4737c4b2cee4153ed6db87802cc2211a0280bb92f890afc983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Applied Microbiology</topic><topic>Bacterial Proteins - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Chickens</topic><topic>DNA Gyrase - genetics</topic><topic>Drug resistance</topic><topic>Drug Resistance, Bacterial</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Fast food</topic><topic>Food</topic><topic>Food Microbiology</topic><topic>Humans</topic><topic>Immunology</topic><topic>Life Sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Molecular biology</topic><topic>Molecular Typing</topic><topic>Mutation</topic><topic>Polymorphism</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Poultry</topic><topic>Poultry Diseases - microbiology</topic><topic>Salmonella</topic><topic>Salmonella enteritidis</topic><topic>Salmonella enteritidis - classification</topic><topic>Salmonella enteritidis - drug effects</topic><topic>Salmonella enteritidis - genetics</topic><topic>Salmonella enteritidis - isolation & purification</topic><topic>Salmonella Infections - microbiology</topic><topic>Salmonella Infections, Animal - microbiology</topic><topic>Serbia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kozoderović, G.</creatorcontrib><creatorcontrib>Velhner, M.</creatorcontrib><creatorcontrib>Jelesić, Z.</creatorcontrib><creatorcontrib>Stojanov, I.</creatorcontrib><creatorcontrib>Petrović, T.</creatorcontrib><creatorcontrib>Stojanović, D.</creatorcontrib><creatorcontrib>Golić, N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kozoderović, G.</au><au>Velhner, M.</au><au>Jelesić, Z.</au><au>Stojanov, I.</au><au>Petrović, T.</au><au>Stojanović, D.</au><au>Golić, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia</atitle><jtitle>Folia microbiologica</jtitle><stitle>Folia Microbiol</stitle><addtitle>Folia Microbiol (Praha)</addtitle><date>2011</date><risdate>2011</risdate><volume>56</volume><issue>1</issue><spage>66</spage><epage>71</epage><pages>66-71</pages><issn>0015-5632</issn><eissn>1874-9356</eissn><abstract>Molecular typing and resistotyping coupled with
gyrA
single nucleotide polymorphism (SNP) of 60
Salmonella
Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (
D
) was 0.688. In combination with resistotyping and
gyrA
SNP,
D
increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp
87
→ Asn in six strains, Asp
87
→ Gly in one strain, and Ser
83
→ Phe in one strain. One NAL-resistant strain had no mutations in QRDR, suggesting another mechanism of resistance.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21394475</pmid><doi>10.1007/s12223-011-0003-7</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0015-5632 |
| ispartof | Folia microbiologica, 2011, Vol.56 (1), p.66-71 |
| issn | 0015-5632 1874-9356 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_907161286 |
| source | MEDLINE; Springer Online Journals |
| subjects | Animals Anti-Bacterial Agents - pharmacology Antibiotics Applied Microbiology Bacterial Proteins - genetics Biomedical and Life Sciences Chickens DNA Gyrase - genetics Drug resistance Drug Resistance, Bacterial Environmental Engineering/Biotechnology Fast food Food Food Microbiology Humans Immunology Life Sciences Microbial Sensitivity Tests Microbiology Molecular biology Molecular Typing Mutation Polymorphism Polymorphism, Single Nucleotide Poultry Poultry Diseases - microbiology Salmonella Salmonella enteritidis Salmonella enteritidis - classification Salmonella enteritidis - drug effects Salmonella enteritidis - genetics Salmonella enteritidis - isolation & purification Salmonella Infections - microbiology Salmonella Infections, Animal - microbiology Serbia |
| title | Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T16%3A32%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20typing%20and%20antimicrobial%20resistance%20of%20Salmonella%20Enteritidis%20isolated%20from%20poultry,%20food,%20and%20humans%20in%20Serbia&rft.jtitle=Folia%20microbiologica&rft.au=Kozoderovi%C4%87,%20G.&rft.date=2011&rft.volume=56&rft.issue=1&rft.spage=66&rft.epage=71&rft.pages=66-71&rft.issn=0015-5632&rft.eissn=1874-9356&rft_id=info:doi/10.1007/s12223-011-0003-7&rft_dat=%3Cproquest_cross%3E2377071461%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=872172811&rft_id=info:pmid/21394475&rfr_iscdi=true |