Nasal carriage of Staphylococcus aureus in healthy humans with different levels of contact with animals in Tunisia: genetic lineages, methicillin resistance, and virulence factors
Nasal swabs of 423 healthy humans who showed different levels of contact with animals (frequent, 168; sporadic, 94; no contact, 161) were obtained in Tunisia (2008-2009), and 99 of them presented other associated risk factors. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in one of...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2011-04, Vol.30 (4), p.499-508 |
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| creator | Ben Slama, K Gharsa, H Klibi, N Jouini, A Lozano, C Gómez-Sanz, E Zarazaga, M Boudabous, A Torres, C |
| description | Nasal swabs of 423 healthy humans who showed different levels of contact with animals (frequent, 168; sporadic, 94; no contact, 161) were obtained in Tunisia (2008-2009), and 99 of them presented other associated risk factors. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in one of these 423 samples (0.24%), retrieved from a veterinarian. The MRSA isolate was mecA-positive, typed as ST80-t203-SCCmecIVc-agrIII, and contained tet(K), ant(6)-Ia, and aph(3′)-IIIa genes encoding tetracycline, streptomycin, and kanamycin resistance, respectively. This MRSA isolate also contained the lukF/lukS virulence gene encoding Panton-Valentine leukocidin. Fifty-four (12.8%) additional nasal samples contained methicillin-susceptible S. aureus (MSSA) and one isolate/sample was characterized. A high diversity of spa types (n = 43; 4 new) and pulsed-field gel electrophoresis (PFGE) types (n = 37) was detected among the 55 recovered S. aureus strains. The percentages of antimicrobial resistance/detected resistance genes were as follows: tetracycline [22%/tet(K)-tet(L)-tet(M)], erythromycin [5%/msrA], ciprofloxacin [14.5%], trimethoprim-sulfamethoxazole [2%/dfrA], streptomycin [11%/ant(6)-Ia], kanamycin [7%/aph(3′)-IIIa], amikacin [5%], and chloramphenicol [2%]. Four and two isolates carried the lukF/lukS and eta and/or etb genes, respectively, and always in individuals with contact with animals. Eleven isolates carried the tst gene and were recovered from individuals with different levels of contact with animals. |
| doi_str_mv | 10.1007/s10096-010-1109-6 |
| format | Article |
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Methicillin-resistant Staphylococcus aureus (MRSA) was detected in one of these 423 samples (0.24%), retrieved from a veterinarian. The MRSA isolate was mecA-positive, typed as ST80-t203-SCCmecIVc-agrIII, and contained tet(K), ant(6)-Ia, and aph(3′)-IIIa genes encoding tetracycline, streptomycin, and kanamycin resistance, respectively. This MRSA isolate also contained the lukF/lukS virulence gene encoding Panton-Valentine leukocidin. Fifty-four (12.8%) additional nasal samples contained methicillin-susceptible S. aureus (MSSA) and one isolate/sample was characterized. A high diversity of spa types (n = 43; 4 new) and pulsed-field gel electrophoresis (PFGE) types (n = 37) was detected among the 55 recovered S. aureus strains. The percentages of antimicrobial resistance/detected resistance genes were as follows: tetracycline [22%/tet(K)-tet(L)-tet(M)], erythromycin [5%/msrA], ciprofloxacin [14.5%], trimethoprim-sulfamethoxazole [2%/dfrA], streptomycin [11%/ant(6)-Ia], kanamycin [7%/aph(3′)-IIIa], amikacin [5%], and chloramphenicol [2%]. Four and two isolates carried the lukF/lukS and eta and/or etb genes, respectively, and always in individuals with contact with animals. Eleven isolates carried the tst gene and were recovered from individuals with different levels of contact with animals.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-010-1109-6</identifier><identifier>PMID: 21076928</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Amikacin ; Animals ; Antibacterial agents ; Antibiotic resistance ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antimicrobial agents ; Bacterial diseases ; Bacterial Proteins - genetics ; Bacterial Typing Techniques ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Carrier State - epidemiology ; Carrier State - microbiology ; Chloramphenicol ; Ciprofloxacin ; Drug resistance ; Drug Resistance, Multiple, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field ; Erythromycin ; Genes ; Human bacterial diseases ; Humans ; Infectious diseases ; Internal Medicine ; Kanamycin ; leukocidin ; Medical Microbiology ; Medical sciences ; Methicillin ; Methicillin - pharmacology ; Methicillin Resistance - genetics ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; Methicillin-Resistant Staphylococcus aureus - pathogenicity ; Microbial Sensitivity Tests ; Nasal Mucosa - microbiology ; Nosocomial infections ; Pharmacology. Drug treatments ; Pneumonia ; Pulsed-field gel electrophoresis ; Risk Factors ; Staphylococcal enterotoxin F ; Staphylococcal Infections - epidemiology ; Staphylococcal Infections - microbiology ; Staphylococcal infections, streptococcal infections, pneumococcal infections ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification ; Staphylococcus aureus - pathogenicity ; Staphylococcus infections ; Streptomycin ; Tetracyclines ; trimethoprim-sulfamethoxazole ; Tunisia - epidemiology ; Veterinary medicine ; Veterinary surgeons ; Virulence ; virulence factors ; Virulence Factors - genetics</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2011-04, Vol.30 (4), p.499-508</ispartof><rights>Springer-Verlag 2010</rights><rights>2015 INIST-CNRS</rights><rights>Springer-Verlag 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-7a19e0b8b66dfe545627def2a8476ee69ac70c7e9f55abe26db8b67e49f75fc23</citedby><cites>FETCH-LOGICAL-c499t-7a19e0b8b66dfe545627def2a8476ee69ac70c7e9f55abe26db8b67e49f75fc23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10096-010-1109-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10096-010-1109-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23968496$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21076928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ben Slama, K</creatorcontrib><creatorcontrib>Gharsa, H</creatorcontrib><creatorcontrib>Klibi, N</creatorcontrib><creatorcontrib>Jouini, A</creatorcontrib><creatorcontrib>Lozano, C</creatorcontrib><creatorcontrib>Gómez-Sanz, E</creatorcontrib><creatorcontrib>Zarazaga, M</creatorcontrib><creatorcontrib>Boudabous, A</creatorcontrib><creatorcontrib>Torres, C</creatorcontrib><title>Nasal carriage of Staphylococcus aureus in healthy humans with different levels of contact with animals in Tunisia: genetic lineages, methicillin resistance, and virulence factors</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>Nasal swabs of 423 healthy humans who showed different levels of contact with animals (frequent, 168; sporadic, 94; no contact, 161) were obtained in Tunisia (2008-2009), and 99 of them presented other associated risk factors. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in one of these 423 samples (0.24%), retrieved from a veterinarian. The MRSA isolate was mecA-positive, typed as ST80-t203-SCCmecIVc-agrIII, and contained tet(K), ant(6)-Ia, and aph(3′)-IIIa genes encoding tetracycline, streptomycin, and kanamycin resistance, respectively. This MRSA isolate also contained the lukF/lukS virulence gene encoding Panton-Valentine leukocidin. Fifty-four (12.8%) additional nasal samples contained methicillin-susceptible S. aureus (MSSA) and one isolate/sample was characterized. A high diversity of spa types (n = 43; 4 new) and pulsed-field gel electrophoresis (PFGE) types (n = 37) was detected among the 55 recovered S. aureus strains. The percentages of antimicrobial resistance/detected resistance genes were as follows: tetracycline [22%/tet(K)-tet(L)-tet(M)], erythromycin [5%/msrA], ciprofloxacin [14.5%], trimethoprim-sulfamethoxazole [2%/dfrA], streptomycin [11%/ant(6)-Ia], kanamycin [7%/aph(3′)-IIIa], amikacin [5%], and chloramphenicol [2%]. Four and two isolates carried the lukF/lukS and eta and/or etb genes, respectively, and always in individuals with contact with animals. Eleven isolates carried the tst gene and were recovered from individuals with different levels of contact with animals.</description><subject>Amikacin</subject><subject>Animals</subject><subject>Antibacterial agents</subject><subject>Antibiotic resistance</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antimicrobial agents</subject><subject>Bacterial diseases</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Typing Techniques</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Carrier State - epidemiology</subject><subject>Carrier State - microbiology</subject><subject>Chloramphenicol</subject><subject>Ciprofloxacin</subject><subject>Drug resistance</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Erythromycin</subject><subject>Genes</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>Kanamycin</subject><subject>leukocidin</subject><subject>Medical Microbiology</subject><subject>Medical sciences</subject><subject>Methicillin</subject><subject>Methicillin - pharmacology</subject><subject>Methicillin Resistance - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - isolation & purification</subject><subject>Methicillin-Resistant Staphylococcus aureus - pathogenicity</subject><subject>Microbial Sensitivity Tests</subject><subject>Nasal Mucosa - microbiology</subject><subject>Nosocomial infections</subject><subject>Pharmacology. Drug treatments</subject><subject>Pneumonia</subject><subject>Pulsed-field gel electrophoresis</subject><subject>Risk Factors</subject><subject>Staphylococcal enterotoxin F</subject><subject>Staphylococcal Infections - epidemiology</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal infections, streptococcal infections, pneumococcal infections</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Staphylococcus aureus - pathogenicity</subject><subject>Staphylococcus infections</subject><subject>Streptomycin</subject><subject>Tetracyclines</subject><subject>trimethoprim-sulfamethoxazole</subject><subject>Tunisia - epidemiology</subject><subject>Veterinary medicine</subject><subject>Veterinary surgeons</subject><subject>Virulence</subject><subject>virulence factors</subject><subject>Virulence Factors - genetics</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1u1DAUhSMEokPhAdiAhYTYNGAnsR2zQxV_UgWLtuvojnM9ceU4g-0UzXPxgjhkoBIL2PjK9nfOvfYpiqeMvmaUyjcxr0qUlNGSMapKca_YsKbmZVPL-n6xoapuSiWr-qR4FOMNzZpWyofFScWoFKpqN8WPLxDBEQ0hWNghmQy5TLAfDm7Sk9ZzJDAHzMV6MiC4NBzIMI_gI_lu00B6awwG9Ik4vEUXFwM9-QQ6rQB4O4L7pb-avY0W3pIdekxWE2c95qbxjIyYBqutyyckYLQxgdd4ltU9ubVhdpi3xGTXKcTHxQOTLfHJsZ4W1x_eX51_Ki--fvx8_u6i1I1SqZTAFNJtuxWiN8gbLirZo6mgbaRAFAq0pFqiMpzDFivRL6zERhnJja7q0-LV6rsP07cZY-pGGzU6Bx6nOXaKSsaVVOq_ZMuFFJIxmckXf5E30xx8fsYCsTYnyjPEVkiHKcaAptuH_Ivh0DHaLcl3a_IdXfY5-U5kzbOj8bwdsf-j-B11Bl4eAYganAn5h22842ol2kYtRtXKxXzldxjuJvxX9-eryMDUwS5k4-vLirKaMsWbPEH9E_w40wY</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Ben Slama, K</creator><creator>Gharsa, H</creator><creator>Klibi, N</creator><creator>Jouini, A</creator><creator>Lozano, C</creator><creator>Gómez-Sanz, E</creator><creator>Zarazaga, M</creator><creator>Boudabous, A</creator><creator>Torres, C</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20110401</creationdate><title>Nasal carriage of Staphylococcus aureus in healthy humans with different levels of contact with animals in Tunisia: genetic lineages, methicillin resistance, and virulence factors</title><author>Ben Slama, K ; Gharsa, H ; Klibi, N ; Jouini, A ; Lozano, C ; Gómez-Sanz, E ; Zarazaga, M ; Boudabous, A ; Torres, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-7a19e0b8b66dfe545627def2a8476ee69ac70c7e9f55abe26db8b67e49f75fc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amikacin</topic><topic>Animals</topic><topic>Antibacterial agents</topic><topic>Antibiotic resistance</topic><topic>Antibiotics. 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Antiparasitic agents</topic><topic>Antimicrobial agents</topic><topic>Bacterial diseases</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Typing Techniques</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Carrier State - epidemiology</topic><topic>Carrier State - microbiology</topic><topic>Chloramphenicol</topic><topic>Ciprofloxacin</topic><topic>Drug resistance</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Erythromycin</topic><topic>Genes</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Internal Medicine</topic><topic>Kanamycin</topic><topic>leukocidin</topic><topic>Medical Microbiology</topic><topic>Medical sciences</topic><topic>Methicillin</topic><topic>Methicillin - pharmacology</topic><topic>Methicillin Resistance - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - isolation & purification</topic><topic>Methicillin-Resistant Staphylococcus aureus - pathogenicity</topic><topic>Microbial Sensitivity Tests</topic><topic>Nasal Mucosa - microbiology</topic><topic>Nosocomial infections</topic><topic>Pharmacology. 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Methicillin-resistant Staphylococcus aureus (MRSA) was detected in one of these 423 samples (0.24%), retrieved from a veterinarian. The MRSA isolate was mecA-positive, typed as ST80-t203-SCCmecIVc-agrIII, and contained tet(K), ant(6)-Ia, and aph(3′)-IIIa genes encoding tetracycline, streptomycin, and kanamycin resistance, respectively. This MRSA isolate also contained the lukF/lukS virulence gene encoding Panton-Valentine leukocidin. Fifty-four (12.8%) additional nasal samples contained methicillin-susceptible S. aureus (MSSA) and one isolate/sample was characterized. A high diversity of spa types (n = 43; 4 new) and pulsed-field gel electrophoresis (PFGE) types (n = 37) was detected among the 55 recovered S. aureus strains. The percentages of antimicrobial resistance/detected resistance genes were as follows: tetracycline [22%/tet(K)-tet(L)-tet(M)], erythromycin [5%/msrA], ciprofloxacin [14.5%], trimethoprim-sulfamethoxazole [2%/dfrA], streptomycin [11%/ant(6)-Ia], kanamycin [7%/aph(3′)-IIIa], amikacin [5%], and chloramphenicol [2%]. Four and two isolates carried the lukF/lukS and eta and/or etb genes, respectively, and always in individuals with contact with animals. Eleven isolates carried the tst gene and were recovered from individuals with different levels of contact with animals.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>21076928</pmid><doi>10.1007/s10096-010-1109-6</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European journal of clinical microbiology & infectious diseases, 2011-04, Vol.30 (4), p.499-508 |
| issn | 0934-9723 1435-4373 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_907159799 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Amikacin Animals Antibacterial agents Antibiotic resistance Antibiotics. Antiinfectious agents. Antiparasitic agents Antimicrobial agents Bacterial diseases Bacterial Proteins - genetics Bacterial Typing Techniques Biological and medical sciences Biomedical and Life Sciences Biomedicine Carrier State - epidemiology Carrier State - microbiology Chloramphenicol Ciprofloxacin Drug resistance Drug Resistance, Multiple, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Erythromycin Genes Human bacterial diseases Humans Infectious diseases Internal Medicine Kanamycin leukocidin Medical Microbiology Medical sciences Methicillin Methicillin - pharmacology Methicillin Resistance - genetics Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - isolation & purification Methicillin-Resistant Staphylococcus aureus - pathogenicity Microbial Sensitivity Tests Nasal Mucosa - microbiology Nosocomial infections Pharmacology. Drug treatments Pneumonia Pulsed-field gel electrophoresis Risk Factors Staphylococcal enterotoxin F Staphylococcal Infections - epidemiology Staphylococcal Infections - microbiology Staphylococcal infections, streptococcal infections, pneumococcal infections Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus aureus - pathogenicity Staphylococcus infections Streptomycin Tetracyclines trimethoprim-sulfamethoxazole Tunisia - epidemiology Veterinary medicine Veterinary surgeons Virulence virulence factors Virulence Factors - genetics |
| title | Nasal carriage of Staphylococcus aureus in healthy humans with different levels of contact with animals in Tunisia: genetic lineages, methicillin resistance, and virulence factors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T06%3A17%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nasal%20carriage%20of%20Staphylococcus%20aureus%20in%20healthy%20humans%20with%20different%20levels%20of%20contact%20with%20animals%20in%20Tunisia:%20genetic%20lineages,%20methicillin%20resistance,%20and%20virulence%20factors&rft.jtitle=European%20journal%20of%20clinical%20microbiology%20&%20infectious%20diseases&rft.au=Ben%20Slama,%20K&rft.date=2011-04-01&rft.volume=30&rft.issue=4&rft.spage=499&rft.epage=508&rft.pages=499-508&rft.issn=0934-9723&rft.eissn=1435-4373&rft_id=info:doi/10.1007/s10096-010-1109-6&rft_dat=%3Cproquest_cross%3E2287986251%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=856181005&rft_id=info:pmid/21076928&rfr_iscdi=true |